{"data":{"id":"10.6084/m9.figshare.1263587.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1263587.v1","prefix":"10.6084","suffix":"m9.figshare.1263587.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Md Almamun","affiliation":[],"nameIdentifiers":[]},{"name":"Levinson, Benjamin T","nameType":"Personal","givenName":"Benjamin T","familyName":"Levinson","affiliation":[],"nameIdentifiers":[]},{"name":"Gater, Susan T","nameType":"Personal","givenName":"Susan T","familyName":"Gater","affiliation":[],"nameIdentifiers":[]},{"name":"Schnabel, Robert D","nameType":"Personal","givenName":"Robert D","familyName":"Schnabel","affiliation":[],"nameIdentifiers":[]},{"name":"Arthur, Gerald L","nameType":"Personal","givenName":"Gerald L","familyName":"Arthur","affiliation":[],"nameIdentifiers":[]},{"name":"J Wade Davis","affiliation":[],"nameIdentifiers":[]},{"name":"Taylor, Kristen H","nameType":"Personal","givenName":"Kristen H","familyName":"Taylor","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Genome-wide DNA methylation analysis in precursor B-cells"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2014,"subjects":[{"subject":"Plant Biology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Hematology"},{"subject":"Developmental Biology"},{"subject":"Biological Sciences"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"Cell Biology"},{"subject":"Biochemistry"},{"subject":"Biophysics"}],"contributors":[],"dates":[{"date":"2014-12-02","dateType":"Created"},{"date":"2016-01-19","dateType":"Updated"},{"date":"2014","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.4161/15592294.2014.983379","relatedIdentifierType":"DOI"},{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.1263587","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["1934712 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"DNA methylation is responsible for regulating gene expression and cellular differentiation and for maintaining genomic stability during normal human development. Furthermore, it plays a significant role in the regulation of hematopoiesis. In order to elucidate the influence of DNA methylation during B-cell development, genome-wide DNA methylation status of pro-B, pre-BI, pre-BII, and naïve-B-cells isolated from human umbilical cord blood was determined using the methylated CpG island recovery assay followed by next generation sequencing. On average, 182 – 200 million sequences were generated for each precursor B-cell subset in 10 biological replicates. An overall decrease in methylation was observed during the transition from pro-B to pre-BI, whereas no differential methylation was observed in the pre-BI to pre-BII transition or in the pre-BII to naïve B-cell transition. Most of the methylated regions were located within intergenic and intronic regions not present in a CpG island context. Putative novel enhancers were identified in these regions that were differentially methylated between pro-B and pre-BI cells. The genome-wide methylation profiles are publically available and may be used to gain a better understanding of the involvement of atypical DNA methylation in the pathogenesis of malignancies associated with precursor B-cells.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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