{"data":{"id":"10.6084/m9.figshare.1254811.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1254811.v1","prefix":"10.6084","suffix":"m9.figshare.1254811.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Kondo, Akihiko","nameType":"Personal","givenName":"Akihiko","familyName":"Kondo","affiliation":[],"nameIdentifiers":[]},{"name":"Matsuda, Mami","nameType":"Personal","givenName":"Mami","familyName":"Matsuda","affiliation":[],"nameIdentifiers":[]},{"name":"Ginga Shimakawa","affiliation":[],"nameIdentifiers":[]},{"name":"Hasunuma, Tomohisa","nameType":"Personal","givenName":"Tomohisa","familyName":"Hasunuma","affiliation":[],"nameIdentifiers":[]},{"name":"Amane Makino","affiliation":[],"nameIdentifiers":[]},{"name":"Chikahiro Miyake","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Respiration accumulates Calvin cycle intermediates for the rapid start of photosynthesis in \u003ci\u003eSynechocystis\u003c/i\u003e sp. PCC 6803"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2014,"subjects":[{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Science Policy"},{"subject":"Marine Biology"},{"subject":"Biological Sciences"},{"subject":"Ecology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Earth and Environmental Sciences"},{"subject":"Genetics"},{"subject":"Cell Biology"},{"subject":"Medicine"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2014-12-01","dateType":"Created"},{"date":"2016-01-19","dateType":"Updated"},{"date":"2014","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/09168451.2014.943648","relatedIdentifierType":"DOI"},{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.1254811","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["71668 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"We tested the hypothesis that inducing photosynthesis in cyanobacteria requires respiration. A mutant deficient in glycogen phosphorylase (∆GlgP) was prepared in \u003ci\u003eSynechocystis\u003c/i\u003e sp. PCC 6803 to suppress respiration. The accumulated glycogen in ΔGlgP was 250–450% of that accumulated in wild type (WT). The rate of dark respiration in ΔGlgP was 25% of that in WT. In the dark, P700\u003csup\u003e+\u003c/sup\u003e reduction was suppressed in ΔGlgP, and the rate corresponded to that in (2,5-dibromo-3-methyl-6-isopropyl-\u003ci\u003ep\u003c/i\u003e-benzoquinone)-treated WT, supporting a lower respiration rate in ∆GlgP. Photosynthetic O\u003csub\u003e2\u003c/sub\u003e-evolution rate reached a steady-state value much slower in ∆GlgP than in WT. This retardation was solved by addition of d-glucose. Furthermore, we found that the contents of Calvin cycle intermediates in ∆GlgP were lower than those in WT under dark conditions. These observations indicated that respiration provided the carbon source for regeneration of ribulose 1,5-bisphosphate in order to drive the rapid start of photosynthesis.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Respiration_accumulates_Calvin_cycle_intermediates_for_the_rapid_start_of_photosynthesis_in_i_Synechocystis_i_sp_PCC_6803/1254811/1","c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