{"data":{"id":"10.6084/m9.figshare.1252229.v3","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1252229.v3","prefix":"10.6084","suffix":"m9.figshare.1252229.v3","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Rovetta, Ana I","nameType":"Personal","givenName":"Ana I","familyName":"Rovetta","affiliation":[],"nameIdentifiers":[]},{"name":"Peña, Delfina","nameType":"Personal","givenName":"Delfina","familyName":"Peña","affiliation":[],"nameIdentifiers":[]},{"name":"Pino, Rodrigo E Hernández Del","nameType":"Personal","givenName":"Rodrigo E Hernández Del","familyName":"Pino","affiliation":[],"nameIdentifiers":[]},{"name":"Recalde, Gabriela M","nameType":"Personal","givenName":"Gabriela M","familyName":"Recalde","affiliation":[],"nameIdentifiers":[]},{"name":"Pellegrini, Joaquín","nameType":"Personal","givenName":"Joaquín","familyName":"Pellegrini","affiliation":[],"nameIdentifiers":[]},{"name":"Bigi, Fabiana","nameType":"Personal","givenName":"Fabiana","familyName":"Bigi","affiliation":[],"nameIdentifiers":[]},{"name":"Musella, Rosa M","nameType":"Personal","givenName":"Rosa M","familyName":"Musella","affiliation":[],"nameIdentifiers":[]},{"name":"Palmero, Domingo J","nameType":"Personal","givenName":"Domingo J","familyName":"Palmero","affiliation":[],"nameIdentifiers":[]},{"name":"Gutierrez, Marisa","nameType":"Personal","givenName":"Marisa","familyName":"Gutierrez","affiliation":[],"nameIdentifiers":[]},{"name":"Colombo, María I","nameType":"Personal","givenName":"María I","familyName":"Colombo","affiliation":[],"nameIdentifiers":[]},{"name":"García, Verónica E","nameType":"Personal","givenName":"Verónica E","familyName":"García","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"IFNG-mediated immune responses enhance autophagy against \u003ci\u003eMycobacterium tuberculosis\u003c/i\u003e antigens in patients with active tuberculosis"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Cancer"},{"subject":"Developmental Biology"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Physiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Microbiology"}],"contributors":[],"dates":[{"date":"2015-06-09","dateType":"Created"},{"date":"2016-01-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.4161/15548627.2014.981791","relatedIdentifierType":"DOI"},{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.1252229","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["842561 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Protective immunity against \u003ci\u003eMycobacterium tuberculosis\u003c/i\u003e (\u003ci\u003eMtb\u003c/i\u003e) requires IFNG. Besides, IFNG-mediated induction of autophagy suppresses survival of virulent \u003ci\u003eMtb\u003c/i\u003e in macrophage cell lines. We investigated the contribution of autophagy to the defense against \u003ci\u003eMtb\u003c/i\u003e antigen (\u003ci\u003eMtb\u003c/i\u003e-Ag) in cells from tuberculosis patients and healthy donors (HD). Patients were classified as high responders (HR) if their T cells produced significant IFNG against \u003ci\u003eMtb\u003c/i\u003e-Ag; and low responders (LR) when patients showed weak or no T cell responses to \u003ci\u003eMtb\u003c/i\u003e-Ag. The highest autophagy levels were detected in HD cells whereas the lowest quantities were observed in LR patients. Interestingly, upon \u003ci\u003eMtb-\u003c/i\u003eAg stimulation, we detected a positive correlation between IFNG and MAP1LC3B-II/LC3-II levels. Actually, blockage of \u003ci\u003eMtb\u003c/i\u003e-Ag-induced IFNG markedly reduced autophagy in HR patients whereas addition of limited amounts of IFNG significantly increased autophagy in LR patients. Therefore, autophagy collaborates with human immune responses against \u003ci\u003eMtb\u003c/i\u003e in close association with specific IFNG secreted against the pathogen.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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