{"data":{"id":"10.6084/m9.figshare.1248827.v2","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1248827.v2","prefix":"10.6084","suffix":"m9.figshare.1248827.v2","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Yano, Kenichi","nameType":"Personal","givenName":"Kenichi","familyName":"Yano","affiliation":[],"nameIdentifiers":[]},{"name":"Wachi, Masaaki","nameType":"Personal","givenName":"Masaaki","familyName":"Wachi","affiliation":[],"nameIdentifiers":[]},{"name":"Tsuchida, Sakiko","nameType":"Personal","givenName":"Sakiko","familyName":"Tsuchida","affiliation":[],"nameIdentifiers":[]},{"name":"Kitazume, Tomoya","nameType":"Personal","givenName":"Tomoya","familyName":"Kitazume","affiliation":[],"nameIdentifiers":[]},{"name":"Iwai, Noritaka","nameType":"Personal","givenName":"Noritaka","familyName":"Iwai","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Degradation of benzotrifluoride via the dioxygenase pathway in \u003ci\u003eRhodococcus\u003c/i\u003e sp. 065240"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Science Policy"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Earth and Environmental Sciences"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Microbiology"}],"contributors":[],"dates":[{"date":"2015-03-09","dateType":"Created"},{"date":"2016-01-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/09168451.2014.982502","relatedIdentifierType":"DOI"},{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.1248827","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["1485024 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"We previously isolated \u003ci\u003eRhodococcus\u003c/i\u003e sp. 065240, which catalyzes the defluorination of benzotrifluoride (BTF). In order to investigate the mechanism of this degradation of BTF, we performed proteomic analysis of cells grown with or without BTF. Three proteins, which resemble dioxygenase pathway enzymes responsible for isopropylbenzene degradation from \u003ci\u003eRhodococcus erythropolis\u003c/i\u003e BD2, were induced by BTF. Genomic PCR and DNA sequence analysis revealed that the \u003ci\u003eRhodococcus\u003c/i\u003e sp. 065240 carries the gene cluster, \u003ci\u003ebtf\u003c/i\u003e, which is highly homologous to the \u003ci\u003eipb\u003c/i\u003e gene cluster from \u003ci\u003eR. erythropolis\u003c/i\u003e BD2. A mutant strain, which could not catalyze BTF defluorination, was isolated from 065240 strain by UV mutagenesis. The mutant strain had one mutation in the \u003ci\u003ebtfT\u003c/i\u003e gene, which encodes a response regulator of the two component system. The defluorinating ability of the mutant strain was recovered by complementation of \u003ci\u003ebtfT\u003c/i\u003e. These results suggest that the \u003ci\u003ebtf\u003c/i\u003e gene cluster is responsible for degradation of BTF.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Degradation_of_benzotrifluoride_via_the_dioxygenase_pathway_in_i_Rhodococcus_i_sp_065240/1248827/2","contentUrl":null,"metadataVersion":3,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable",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