{"data":{"id":"10.6084/m9.figshare.1246868.v3","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1246868.v3","prefix":"10.6084","suffix":"m9.figshare.1246868.v3","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Higashino, Ayako","nameType":"Personal","givenName":"Ayako","familyName":"Higashino","affiliation":[],"nameIdentifiers":[]},{"name":"Yuh Shiwa","affiliation":[],"nameIdentifiers":[]},{"name":"Yoshikawa, Hirofumi","nameType":"Personal","givenName":"Hirofumi","familyName":"Yoshikawa","affiliation":[],"nameIdentifiers":[]},{"name":"Kokubo, Tetsuro","nameType":"Personal","givenName":"Tetsuro","familyName":"Kokubo","affiliation":[],"nameIdentifiers":[]},{"name":"Kasahara, Koji","nameType":"Personal","givenName":"Koji","familyName":"Kasahara","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Both HMG boxes in Hmo1 are essential for DNA binding \u003ci\u003ein vitro\u003c/i\u003e and \u003ci\u003ein vivo\u003c/i\u003e"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Cancer"},{"subject":"Developmental Biology"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Meta Science"},{"subject":"Chemistry"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Medicine"},{"subject":"Engineering"},{"subject":"Biochemistry"},{"subject":"Biophysics"}],"contributors":[],"dates":[{"date":"2015-03-09","dateType":"Created"},{"date":"2016-01-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/09168451.2014.978258","relatedIdentifierType":"DOI"},{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.1246868","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["387840 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Hmo1, a member of the high mobility group B family proteins in \u003ci\u003eSaccharomyces cerevisiae\u003c/i\u003e, associates with the promoters of ribosomal protein genes (RPGs) to direct accurate transcriptional initiation. Here, to identify factors involved in the binding of Hmo1 to its targets and the mechanism of Hmo1-dependent transcriptional initiation, we developed a novel reporter system using the promoter of the RPG \u003ci\u003eRPS5\u003c/i\u003e. A genetic screen did not identify any factors that influence Hmo1 binding, but did identify a number of mutations in Hmo1 that impair its DNA binding activity \u003ci\u003ein vivo\u003c/i\u003e and \u003ci\u003ein vitro\u003c/i\u003e. These results suggest that Hmo1 binds to its target promoters autonomously without any aid of additional factors. Furthermore, characterization of Hmo1 mutants showed that the box A domain plays a pivotal role in DNA binding and may be required for the recognition of structural properties of target promoters that occur in native chromatin.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Both_HMG_boxes_in_Hmo1_are_essential_for_DNA_binding_i_in_vitro_i_and_i_in_vivo_i_/1246868/3","contentUrl":null,"metadataVersion":2,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"c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