{"data":{"id":"10.6084/m9.figshare.1246726.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1246726.v1","prefix":"10.6084","suffix":"m9.figshare.1246726.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"I. Dobrowolska","affiliation":[],"nameIdentifiers":[]},{"name":"J. Kwasniewska","affiliation":[],"nameIdentifiers":[]},{"name":"P. W. Barlow","affiliation":[],"nameIdentifiers":[]},{"name":"E. U. Kurczynska","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"The fate of surface cell layers of \u003ci\u003eDaucus carota\u003c/i\u003e (L.) embryos raised in suspension culture"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Hematology"},{"subject":"Cancer"},{"subject":"Developmental Biology"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biotechnology"},{"subject":"Cell Biology"},{"subject":"Microbiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-01-09","dateType":"Created"},{"date":"2016-01-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/11263504.2014.989288","relatedIdentifierType":"DOI"},{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.1246726","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["10157182 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"The ultrastructure and fate of surface cells covering mature somatic embryos of \u003ci\u003eDaucus carota\u003c/i\u003e grown in suspension culture were analyzed and new information obtained concerning somatic embryogenesis in these conditions. Our studies showed that, during some developmental stages, these embryos were covered irregularly and discontinuously by cells with a typical protodermal phenotype characterized by a cuticle on the outer cell wall. We observed that cells with cuticle were peeled off from the surface of mature embryos. Before peeling off, these cells underwent programmed cell death (PCD), which was confirmed by the TUNEL method. Transmission electron microscopy revealed advanced processes of autophagy in these cells.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/The_fate_of_surface_cell_layers_of_i_Daucus_carota_i_L_embryos_raised_in_suspension_culture/1246726/1","contentUrl":null,"metadataVersion":5,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2016-01-19T11:03:37.000Z","registered":"2016-01-19T11:03:38.000Z","published":"2015","updated":"2020-09-04T20:25:47.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.1246726.v1","type":"media"}},"references":{"data":[]},"citations":{"data":[]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}