{"data":{"id":"10.6084/m9.figshare.1245030.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1245030.v1","prefix":"10.6084","suffix":"m9.figshare.1245030.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Müller, Henrik","nameType":"Personal","givenName":"Henrik","familyName":"Müller","affiliation":[],"nameIdentifiers":[]},{"name":"Brener, Oleksandr","nameType":"Personal","givenName":"Oleksandr","familyName":"Brener","affiliation":[],"nameIdentifiers":[]},{"name":"Andreoletti, Olivier","nameType":"Personal","givenName":"Olivier","familyName":"Andreoletti","affiliation":[],"nameIdentifiers":[]},{"name":"Piechatzek, Timo","nameType":"Personal","givenName":"Timo","familyName":"Piechatzek","affiliation":[],"nameIdentifiers":[]},{"name":"Willbold, Dieter","nameType":"Personal","givenName":"Dieter","familyName":"Willbold","affiliation":[],"nameIdentifiers":[]},{"name":"Legname, Giuseppe","nameType":"Personal","givenName":"Giuseppe","familyName":"Legname","affiliation":[],"nameIdentifiers":[]},{"name":"Heise, Henrike","nameType":"Personal","givenName":"Henrike","familyName":"Heise","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Progress towards structural understanding of infectious sheep PrP-amyloid"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2014,"subjects":[{"subject":"Computational Biology"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biological Sciences"},{"subject":"Chemistry"},{"subject":"Neuroscience"},{"subject":"Biochemistry"},{"subject":"Biophysics"}],"contributors":[],"dates":[{"date":"2014-09-03","dateType":"Created"},{"date":"2016-01-19","dateType":"Updated"},{"date":"2014","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.4161/19336896.2014.983754","relatedIdentifierType":"DOI"},{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.1245030","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["6510612 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"ABSTRACTThe still elusive structural difference of non-infectious and infectious amyloid of the mammalian prion protein (PrP) is a major pending milestone in understanding protein-mediated infectivity in neurodegenerative diseases. Preparations of PrP-amyloid proven to be infectious have never been investigated with a high-resolution technique. All available models to date have been based on low-resolution data. Here, we establish protocols for the preparation of infectious samples of full-length recombinant (rec) PrP\u003csup\u003e−\u003c/sup\u003eamyloid in NMR-sufficient amounts by spontaneous fibrillation and seeded fibril growth from brain extract. We link biological and structural data of infectious recPrP-amyloid, derived from bioassays, atomic force microscopy, and solid-state NMR spectroscopy. Our data indicate a semi-mobile N-terminus, some residues with secondary chemical shifts typical of α-helical secondary structure in the middle part between ∼115 to ∼155, and a distinct β-sheet core C-terminal of residue ∼155. These findings are not in agreement with all current models for PrP-amyloid. We also provide evidence that samples seeded from brain extract may not differ in the overall arrangement of secondary structure elements, but rather in the flexibility of protein segments outside the β-core region. Taken together, our protocols provide an essential basis for the high-resolution characterisation of non-infectious and infectious PrP-amyloid in the near future.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Progress_towards_structural_understanding_of_infectious_sheep_PrP_amyloid/1245030/1","contentUrl":null,"metadataVersion":3,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2016-01-19T11:00:56.000Z","registered":"2016-01-19T11:00:57.000Z","published":"2014","updated":"2020-09-04T20:26:47.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.1245030.v1","type":"media"}},"references":{"data":[]},"citations":{"data":[]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}