{"data":{"id":"10.6084/m9.figshare.1239067","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1239067","prefix":"10.6084","suffix":"m9.figshare.1239067","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Teck Yew Low","affiliation":[],"nameIdentifiers":[]},{"name":"Magliozzi, Roberto","nameType":"Personal","givenName":"Roberto","familyName":"Magliozzi","affiliation":[],"nameIdentifiers":[]},{"name":"Shabaz Mohammed","affiliation":[],"nameIdentifiers":[]},{"name":"Guardavaccaro, Daniele","nameType":"Personal","givenName":"Daniele","familyName":"Guardavaccaro","affiliation":[],"nameIdentifiers":[]},{"name":"Albert JR Heck","nameType":"Personal","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"The Quest for Substrates for SCF -Trcp2 Ubiquitin Ligase: Crosstalk between Phosphorylation and Ubiquitination"}],"publisher":"figshare","container":{},"publicationYear":2014,"subjects":[{"subject":"60102 Bioinformatics","subjectScheme":"FOR"},{"subject":"FOS: Computer and information sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Computer and information sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biochemistry"},{"subject":"60114 Systems Biology","subjectScheme":"FOR"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biological Techniques"}],"contributors":[],"dates":[{"date":"2014-11-13","dateType":"Created"},{"date":"2014-11-17","dateType":"Updated"},{"date":"2014","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[],"relatedItems":[],"sizes":["6451000 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"\u003cem\u003epresented in: Keystone Symposia 2011, Keystone Colorado\u003c/em\u003e Poly-ubiquitination is a post-translational modi_cation that marks proteins for proteasomal degradation. In this process, substrates are covalently modi_ed by ubiquitin on speci_c lysine residues by three enzymes sequentially. They are: ubiquitin activating enzymes (E1), ubiquitin-conjugating enzyme (E2) and ubiquitin ligases (E3). It is the E3 enzyme that confers substrate speci_city to the ubiquitin proteasome system. The human genome encodes for approximately thirty three E2 ubiquitin-conjugating enzymes, two E1 ubiquitin activating enzymes and over seven-hundred E3s. The sheer size of the E3s family indicates that there is a huge repertoire of substrates for the ubiquitin-proteasome system. SCF ligases are a class of E3 enzymes composed of Skp1, Cullin 1, a RING-_nger protein (Rbx1 or Rbx2) and a variable component known as the F-box protein. The F-box protein family recognizes and captures specific substrates that are subsequently ubiquitinated by E2 ubiquitin-conjugating enzymes. For a number of the well-studied F-box substrates, such as Fbw7, Skp2 and _-Trcp, substrate binding requires the presence of unique phosphorylated motifs (phosphodegrons) on the substrates, indicating crosstalk between phosphorylation and ubiquitination. For instance, β-Trcp is an F-box protein containing WD repeats that bind speci_c substrates haboring the DSpGXX(X)Sp motif.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"PD94bWwgdmVyc2lvbj0iMS4wIiBlbmNvZGluZz0iVVRGLTgiPz4KPHJlc291cmNlIHhtbG5zPSJodHRwOi8vZGF0YWNpdGUub3JnL3NjaGVtYS9rZXJuZWwtNCIgeG1sbnM6eHNpPSJodHRwOi8vd3d3LnczLm9yZy8yMDAxL1hNTFNjaGVtYS1pbnN0YW5jZSIgeHNpOnNjaGVtYUxvY2F0aW9uPSJodHRwOi8vZGF0YWNpdGUub3JnL3NjaGVtYS9rZXJuZWwtNCBodHRwOi8vc2NoZW1hLmRhdGFjaXRlLm9yZy9tZXRhL2tlcm5lbC00LjEvbWV0YWRhdGEueHNkIj4KICA8aWRlbnRpZmllciBpZGVudGlmaWVyVHlwZT0iRE9JIj4xMC42MDg0L005LkZJR1NIQVJFLjEyMzkwNjc8L2lkZW50aWZpZXI+CiAgPGNyZWF0b3JzPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5UZWNrIHlldyBMb3c8L2NyZWF0b3JOYW1lPgogICAgPC9jcmVhdG9yPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5Sb2JlcnRvIE1hZ2xpb3p6aTwvY3JlYXRvck5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPlNoYWJheiBNb2hhbW1lZDwvY3JlYXRvck5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPkRhbmllbGUgR3VhcmRhdmFjY2FybzwvY3JlYXRvck5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPkFsYmVydCBKUiBIZWNrPC9jcmVhdG9yTmFtZT4KICAgIDwvY3JlYXRvcj4KICA8L2NyZWF0b3JzPgogIDx0aXRsZXM+CiAgICA8dGl0bGU+VGhlIFF1ZXN0IGZvciBTdWJzdHJhdGVzIGZvciBTQ0YgLVRyY3AyIFViaXF1aXRpbiBMaWdhc2U6IENyb3NzdGFsayBiZXR3ZWVuIFBob3NwaG9yeWxhdGlvbiBhbmQgVWJpcXVpdGluYXRpb248L3RpdGxlPgogIDwvdGl0bGVzPgogIDxkZXNjcmlwdGlvbnM+CiAgICA8ZGVzY3JpcHRpb24gZGVzY3JpcHRpb25UeXBlPSJBYnN0cmFjdCI+Jmx0O3AmZ3Q7Jmx0O2VtJmd0O3ByZXNlbnRlZCBpbjogS2V5c3RvbmUgU3ltcG9zaWEgMjAxMSwgS2V5c3RvbmUgQ29sb3JhZG8mbHQ7L2VtJmd0OyZsdDsvcCZndDsKJmx0O3AmZ3Q7wqAmbHQ7L3AmZ3Q7CiZsdDtwJmd0O1BvbHktdWJpcXVpdGluYXRpb24gaXMgYSBwb3N0LXRyYW5zbGF0aW9uYWwgbW9kaV9jYXRpb24gdGhhdCBtYXJrcyBwcm90ZWlucyBmb3IgcHJvdGVhc29tYWwgZGVncmFkYXRpb24uIEluIHRoaXMgcHJvY2Vzcywgc3Vic3RyYXRlcyBhcmUgY292YWxlbnRseSBtb2RpX2VkIGJ5IHViaXF1aXRpbiBvbiBzcGVjaV9jIGx5c2luZSByZXNpZHVlcyBieSB0aHJlZSBlbnp5bWVzIHNlcXVlbnRpYWxseS4gVGhleSBhcmU6IHViaXF1aXRpbiBhY3RpdmF0aW5nIGVuenltZXMgKEUxKSwgdWJpcXVpdGluLWNvbmp1Z2F0aW5nIGVuenltZSAoRTIpIGFuZCB1YmlxdWl0aW4gbGlnYXNlcyAoRTMpLiBJdCBpcyB0aGUgRTMgZW56eW1lIHRoYXQgY29uZmVycyBzdWJzdHJhdGUgc3BlY2lfY2l0eSB0byB0aGUgdWJpcXVpdGluIHByb3RlYXNvbWUgc3lzdGVtLiBUaGUgaHVtYW4gZ2Vub21lIGVuY29kZXMgZm9yIGFwcHJveGltYXRlbHkgdGhpcnR5IHRocmVlIEUyIHViaXF1aXRpbi1jb25qdWdhdGluZyBlbnp5bWVzLCB0d28gRTEgdWJpcXVpdGluIGFjdGl2YXRpbmcgZW56eW1lcyBhbmQgb3ZlciBzZXZlbi1odW5kcmVkIEUzcy4gVGhlIHNoZWVyIHNpemUgb2YgdGhlIEUzcyBmYW1pbHkgaW5kaWNhdGVzIHRoYXQgdGhlcmUgaXMgYSBodWdlIHJlcGVydG9pcmUgb2Ygc3Vic3RyYXRlcyBmb3IgdGhlIHViaXF1aXRpbi1wcm90ZWFzb21lIHN5c3RlbS4mbHQ7L3AmZ3Q7CiZsdDtwJmd0O1NDRiBsaWdhc2VzIGFyZSBhIGNsYXNzIG9mIEUzIGVuenltZXMgY29tcG9zZWQgb2YgU2twMSwgQ3VsbGluIDEsIGEgUklORy1fbmdlciBwcm90ZWluIChSYngxIG9yIFJieDIpIGFuZCBhIHZhcmlhYmxlIGNvbXBvbmVudCBrbm93biBhcyB0aGUgRi1ib3ggcHJvdGVpbi4gVGhlIEYtYm94IHByb3RlaW4gZmFtaWx5IHJlY29nbml6ZXMgYW5kIGNhcHR1cmVzIHNwZWNpZmljIHN1YnN0cmF0ZXMgdGhhdCBhcmUgc3Vic2VxdWVudGx5IHViaXF1aXRpbmF0ZWQgYnkgRTIgdWJpcXVpdGluLWNvbmp1Z2F0aW5nIGVuenltZXMuIEZvciBhIG51bWJlciBvZiB0aGUgd2VsbC1zdHVkaWVkIEYtYm94IHN1YnN0cmF0ZXMsIHN1Y2ggYXMgRmJ3NywgU2twMiBhbmQgXy1UcmNwLCBzdWJzdHJhdGUgYmluZGluZyByZXF1aXJlcyB0aGUgcHJlc2VuY2Ugb2YgdW5pcXVlIHBob3NwaG9yeWxhdGVkIG1vdGlmcyAocGhvc3Bob2RlZ3JvbnMpIG9uIHRoZSBzdWJzdHJhdGVzLCBpbmRpY2F0aW5nIGNyb3NzdGFsayBiZXR3ZWVuIHBob3NwaG9yeWxhdGlvbiBhbmQgdWJpcXVpdGluYXRpb24uIEZvciBpbnN0YW5jZSwgzrItVHJjcCBpcyBhbiBGLWJveCBwcm90ZWluIGNvbnRhaW5pbmcgV0QgcmVwZWF0cyB0aGF0IGJpbmQgc3BlY2lfYyBzdWJzdHJhdGVzIGhhYm9yaW5nIHRoZSBEU3BHWFgoWClTcCBtb3RpZi4mbHQ7L3AmZ3Q7PC9kZXNjcmlwdGlvbj4KICA8L2Rlc2NyaXB0aW9ucz4KICA8c3ViamVjdHM+CiAgICA8c3ViamVjdCBzY2hlbWVVUkk9Imh0dHA6Ly93d3cuYWJzLmdvdi5hdS9hdXNzdGF0cy9hYnNALm5zZi8wLzZCQjQyN0FCOTY5NkMyMjVDQTI1NzQxODAwMDQ0NjNFIiBzdWJqZWN0U2NoZW1lPSJGT1IiPjYwMTAyIEJpb2luZm9ybWF0aWNzPC9zdWJqZWN0PgogICAgPHN1YmplY3Q+QmlvY2hlbWlzdHJ5PC9zdWJqZWN0PgogICAgPHN1YmplY3Qgc2NoZW1lVVJJPSJodHRwOi8vd3d3LmFicy5nb3YuYXUvYXVzc3RhdHMvYWJzQC5uc2YvMC82QkI0MjdBQjk2OTZDMjI1Q0EyNTc0MTgwMDA0NDYzRSIgc3ViamVjdFNjaGVtZT0iRk9SIj42MDExNCBTeXN0ZW1zIEJpb2xvZ3k8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5CaW9sb2dpY2FsIFRlY2huaXF1ZXM8L3N1YmplY3Q+CiAgPC9zdWJqZWN0cz4KICA8cHVibGlzaGVyPmZpZ3NoYXJlPC9wdWJsaXNoZXI+CiAgPHB1YmxpY2F0aW9uWWVhcj4yMDE0PC9wdWJsaWNhdGlvblllYXI+CiAgPGRhdGVzPgogICAgPGRhdGUgZGF0ZVR5cGU9IkNyZWF0ZWQiPjIwMTQtMTEtMTM8L2RhdGU+CiAgICA8ZGF0ZSBkYXRlVHlwZT0iVXBkYXRlZCI+MjAxNC0xMS0xNzwvZGF0ZT4KICA8L2RhdGVzPgogIDxyZXNvdXJjZVR5cGUgcmVzb3VyY2VUeXBlR2VuZXJhbD0iRGF0YXNldCI+RGF0YXNldDwvcmVzb3VyY2VUeXBlPgogIDxzaXplcz4KICAgIDxzaXplPjY0NTEwMDAgQnl0ZXM8L3NpemU+CiAgPC9zaXplcz4KICA8cmlnaHRzTGlzdD4KICAgIDxyaWdodHMgcmlnaHRzVVJJPSJodHRwczovL2NyZWF0aXZlY29tbW9ucy5vcmcvbGljZW5zZXMvYnkvNC4wLyI+Q0MgQlkgNC4wPC9yaWdodHM+CiAgPC9yaWdodHNMaXN0Pgo8L3Jlc291cmNlPg==","url":"https://figshare.com/articles/dataset/The_Quest_for_Substrates_for_SCF_Trcp2_Ubiquitin_Ligase_Crosstalk_between_Phosphorylation_and_Ubiquitination/1239067","contentUrl":null,"metadataVersion":1,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOve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