{"data":{"id":"10.6084/m9.figshare.1232088.v4","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1232088.v4","prefix":"10.6084","suffix":"m9.figshare.1232088.v4","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"O’Rourke, Brian","nameType":"Personal","givenName":"Brian","familyName":"O’Rourke","affiliation":[],"nameIdentifiers":[]},{"name":"Chia-Ping Huang Yang","affiliation":[],"nameIdentifiers":[]},{"name":"Sharp, David","nameType":"Personal","givenName":"David","familyName":"Sharp","affiliation":[],"nameIdentifiers":[]},{"name":"Horwitz, Susan Band","nameType":"Personal","givenName":"Susan Band","familyName":"Horwitz","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Eribulin disrupts EB1-microtubule plus-tip complex formation"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Plant Biology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cancer"},{"subject":"Developmental Biology"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Chemistry"},{"subject":"Physiology"},{"subject":"Molecular Biology"},{"subject":"Cell Biology"},{"subject":"Biochemistry"},{"subject":"Biophysics"}],"contributors":[],"dates":[{"date":"2015-11-13","dateType":"Created"},{"date":"2016-01-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.4161/15384101.2014.950143","relatedIdentifierType":"DOI"},{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.1232088","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["51027 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Eribulin mesylate is a synthetic analog of halichondrin B known to bind tubulin and microtubules, specifically at their protein rich plus-ends, thereby dampening microtubule (MT) dynamics, arresting cells in mitosis, and inducing apoptosis. The proteins which bind to the MT plus-end are known as microtubule plus-end tracking proteins (+TIPs) and have been shown to promote MT growth and stabilization. Eribulin's plus-end binding suggests it may compete for binding sites with known +TIP proteins such as End-binding 1 (EB1). To better understand the impact of eribulin plus-end binding in regard to the proteins which normally bind there, cells expressing GFP-EB1 were treated with various concentrations of eribulin. In a concentration dependent manner, GFP-EB1 became dissociated from the MT plus-ends following drug addition. Similar results were found with immuno-stained fixed cells. Cells treated with low concentrations of eribulin also showed decreased ability to migrate, suggesting the decrease in MT dynamics may have a downstream effect. Extended exposure of eribulin to cells leads to total depolymerization of the MT array. Taken together, these data show eribulin effectively disrupts EB1 +TIP complex formation, providing mechanistic insights into the impact of eribulin on MT dynamics.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Eribulin_disrupts_EB1_microtubule_plus_tip_complex_formation/1232088/4","contentUrl":null,"metadataVersion":3,"schema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