{"data":{"id":"10.6084/m9.figshare.1160485","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1160485","prefix":"10.6084","suffix":"m9.figshare.1160485","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Molis, Markus","nameType":"Personal","givenName":"Markus","familyName":"Molis","affiliation":[],"nameIdentifiers":[]},{"name":"Weinberger, Florian","nameType":"Personal","givenName":"Florian","familyName":"Weinberger","affiliation":[],"nameIdentifiers":[]},{"name":"John, Uwe","nameType":"Personal","givenName":"Uwe","familyName":"John","affiliation":[],"nameIdentifiers":[]},{"name":"Flöthe, Carla R.","nameType":"Personal","givenName":"Carla R.","familyName":"Flöthe","affiliation":[],"nameIdentifiers":[]},{"name":"Kruse, Inken","nameType":"Personal","givenName":"Inken","familyName":"Kruse","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Herbivore-induced defence response in the brown seaweed \u003ci\u003eFucus vesiculosus\u003c/i\u003e (Phaeophyceae): temporal pattern and gene expression"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2014,"subjects":[{"subject":"Plant Biology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cancer"},{"subject":"Marine Biology"},{"subject":"Biological Sciences"},{"subject":"Ecology"},{"subject":"Earth and Environmental Sciences"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"Cell Biology"},{"subject":"Microbiology"}],"contributors":[],"dates":[{"date":"2014-09-04","dateType":"Created"},{"date":"2014-09-04","dateType":"Updated"},{"date":"2014","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/09670262.2014.946452","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["3738616 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"This study aimed to reveal the dynamics of inducible anti-herbivory traits in the brown seaweed \u003ci\u003eFucus vesiculosus\u003c/i\u003e, in response to grazing by the isopod \u003ci\u003eIdotea baltica\u003c/i\u003e. As the molecular basis of defence induction in seaweeds is poorly understood, a transcriptomic approach was used to gain insight into cellular processes underlying defence induction and thus promote the mechanistic understanding of anti-herbivory responses in seaweeds. In a 27 day feeding-assayed induction experiment, temporal patterns of the induced anti-herbivory resistance of \u003ci\u003eF. vesiculosus\u003c/i\u003e were assessed under laboratory conditions. Feeding assays were performed at three day intervals, using fresh and reconstituted food. Microarray hybridizations investigating the expression of genes 3 days before, as well as at the same time as, feeding assays revealed a decreased palatability of previously grazed \u003ci\u003eF. vesiculosus\u003c/i\u003e pieces compared with non-grazed control pieces. Despite permanent exposure to grazers, \u003ci\u003eF. vesiculosus\u003c/i\u003e palatability varied over time. Non-grazed \u003ci\u003eF. vesiculosus\u003c/i\u003e pieces were significantly preferred to grazed pieces after 18 and again after 27 days of previous grazing, while their relative palatability for isopods was comparable at all other times during the experiment. Relative to controls, 562/402 genes were ≥ 1.5-fold up-/down-regulated in seaweed pieces that were grazed for 18 days, i.e. when induction of defences was detected in feeding assays. Massive reprogramming of the regulatory expression orchestra (translation, transcription) as well as up-regulation of genes involved in lipid and carbohydrate metabolism, intracellular trafficking, defence and stress response was found. At the same time, down-regulation of photosynthesis was observed in grazed seaweed pieces. This study suggests a high level of temporal variability in induced anti-herbivory traits of \u003ci\u003eF. vesiculosus\u003c/i\u003e and reveals increased expression of genes with putative defensive functions in conjunction with the reallocation of resources from primary to secondary metabolism.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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