{"data":{"id":"10.6084/m9.figshare.1131991","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1131991","prefix":"10.6084","suffix":"m9.figshare.1131991","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Verma, Priyanka","nameType":"Personal","givenName":"Priyanka","familyName":"Verma","affiliation":[],"nameIdentifiers":[]},{"name":"Abhishek Sharma","affiliation":[],"nameIdentifiers":[]},{"name":"Shamshad Ahmad Khan","affiliation":[],"nameIdentifiers":[]},{"name":"Mathur, Ajay Kumar","nameType":"Personal","givenName":"Ajay Kumar","familyName":"Mathur","affiliation":[],"nameIdentifiers":[]},{"name":"Shanker, Karuna","nameType":"Personal","givenName":"Karuna","familyName":"Shanker","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Morphogenetic and chemical stability of long-term maintained \u003ci\u003eAgrobacterium\u003c/i\u003e-mediated transgenic \u003ci\u003eCatharanthus roseus\u003c/i\u003e plants"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2014,"subjects":[{"subject":"60506 Virology","subjectScheme":"FOR"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Plant Biology"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Ecology"},{"subject":"Chemistry"},{"subject":"Biotechnology"},{"subject":"Molecular Biology"},{"subject":"Cell Biology"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2014-08-08","dateType":"Created"},{"date":"2015-12-02","dateType":"Updated"},{"date":"2014","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/14786419.2014.940348","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["2885874 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Transgenic \u003ci\u003eCatharanthus roseus\u003c/i\u003e plants (transgenic Dhawal [DT] and transgenic Nirmal [NT]) obtained from the \u003ci\u003eAgrobacterium tumefaciens\u003c/i\u003e and \u003ci\u003eAgrobacterium\u003c/i\u003e\u003ci\u003erhizognenes\u003c/i\u003e-mediated transformations, respectively, have been maintained \u003ci\u003ein vitro\u003c/i\u003e for 5 years. Plants were studied at regular intervals for various parameters such as plant height, leaf size, multiplication rate, alkaloid profile and presence of marker genes. DT plant gradually lost the \u003ci\u003eGUS\u003c/i\u003e gene expression and it was not detected in the fifth year while NT plant demonstrated the presence of genes \u003ci\u003erol\u003c/i\u003e\u003ci\u003eA\u003c/i\u003e, \u003ci\u003erol\u003c/i\u003e\u003ci\u003eB\u003c/i\u003e and \u003ci\u003erol\u003c/i\u003e\u003ci\u003eC\u003c/i\u003e even in the fifth year, indicating the more stable nature of \u003ci\u003eRi\u003c/i\u003e transgene. Vindoline content in the DT was two times more than in non-transformed control plants. Alkaloid and tryptophan profiles were almost constant during the 5 years. The cluster analysis revealed that the DT plant is more close to the control Nirmal plant followed by NT plant.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Morphogenetic_and_chemical_stability_of_long_term_maintained_i_Agrobacterium_i_mediated_transgenic_i_Catharanthus_roseus_i_plants/1131991","contentUrl":null,"metadataVe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