{"data":{"id":"10.6084/m9.figshare.1117899","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1117899","prefix":"10.6084","suffix":"m9.figshare.1117899","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Krebs, Jordan","nameType":"Personal","givenName":"Jordan","familyName":"Krebs","affiliation":[],"nameIdentifiers":[]},{"name":"Kieffer-Kwon, Philippe","nameType":"Personal","givenName":"Philippe","familyName":"Kieffer-Kwon","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"CRISPR Design Tool and KO Protocol"}],"publisher":"figshare","container":{},"publicationYear":2014,"subjects":[{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Computational Biology"}],"contributors":[],"dates":[{"date":"2014-07-25","dateType":"Created"},{"date":"2016-04-18","dateType":"Updated"},{"date":"2014","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[],"relatedItems":[],"sizes":["353061451 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Excel tool with documentation for designing sgRNAs from the Casellas Lab, NIH. In addition, we have included the full protocol for making KOs in CH12 and mouse ES cells. Philippe found an error in previously updated tool which falsely called some TRUE off-targets as not off-targets. New tool has been fixed - sorry for the inconvenience.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://figshare.com/articles/dataset/CRISPR_Design_Tool/1117899","contentUrl":null,"metadataVersion":10,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2014-07-25T14:27:30.000Z","registered":"2014-07-25T14:27:31.000Z","published":"2014","updated":"2020-09-04T22:09:24.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.1117899","type":"media"}},"references":{"data":[]},"citations":{"data":[]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}