{"data":{"id":"10.6084/m9.figshare.1019902","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1019902","prefix":"10.6084","suffix":"m9.figshare.1019902","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Gensberger, Eva","nameType":"Personal","givenName":"Eva","familyName":"Gensberger","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Effect of different heterotrophic plate count methods on the composition of culturable microbial community"}],"publisher":"figshare","container":{},"publicationYear":2014,"subjects":[{"subject":"Health Care"},{"subject":"Microbiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2014-05-08","dateType":"Created"},{"date":"2014-05-08","dateType":"Updated"},{"date":"2014","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[],"relatedItems":[],"sizes":["21315 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Heterotrophic plate counts (HPC) are routinely determined within the scope of water quality\u003cbr\u003eassessment. However, variable HPC methods with different cultivation conditions are applied.\u003cbr\u003eThese variabilities can have a significant effect on the outcome of the analysis, particularly with\u003cbr\u003eregard to the composition of culturable microbial community. This was demonstrated by testing\u003cbr\u003ecommonly used low and high incubation temperatures (22 and 37°C) as well as high and low\u003cbr\u003enutrient media (yeast extract agar and R2A). The culturable microbial community composition was\u003cbr\u003einvestigated by 16S rRNA gene sequencing and additional statistical analysis (ANOVA permutation\u003cbr\u003etest on CCA and PERMANOVA). Lower temperature(22°C) showed the abundance of naturally\u003cbr\u003eoccurring Pseudomonadaceae and Aeromonadaceae, whereas at high temperature (37°C)\u003cbr\u003enumerous Enterobacteriaceae and Bacilli were identified. The highest biodiversity was detected at\u003cbr\u003elower temperature, especially on R2A medium. The most significant effect of tested cultivation\u003cbr\u003econditions was assigned to temperature (p \u0026lt; 0.01), although media also affected the abundance of\u003cbr\u003ecertain detected bacteria (as seen in the number of operational taxonomic units). These results\u003cbr\u003eendorse the supposition that different temperatures (low and high) should be included into HPC\u003cbr\u003emeasurement, whereas the selection of media should take into account the monitored water\u003cbr\u003esource itself. Accordingly it can be inferred that the HPC method is more suitable for continuous\u003cbr\u003emonitoring of water quality than for single assessments.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://figshare.com/articles/dataset/Effect_of_different_heterotrophic_plate_count_methods_on_the_composition_of_culturable_microbial_community/1019902","contentUrl":null,"metadataVersion":1,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2014-05-08T17:03:14.000Z","registered":"2014-05-08T17:03:15.000Z","published":"2014","updated":"2020-09-04T23:14:52.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.1019902","type":"media"}},"references":{"data":[]},"citations":{"data":[]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[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