{"data":{"id":"10.6084/m9.figshare.c.3607556_d1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.3607556_d1","prefix":"10.6084","suffix":"m9.figshare.c.3607556_d1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Jungwoo Lee","affiliation":[],"nameIdentifiers":[]},{"name":"Heckl, Dirk","nameType":"Personal","givenName":"Dirk","familyName":"Heckl","affiliation":[],"nameIdentifiers":[]},{"name":"Biju Parekkadan","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Additional file 1: Figure S1. of Multiple genetically engineered humanized microenvironments in a single mouse"}],"publisher":"Figshare","container":{},"publicationYear":2016,"subjects":[{"subject":"Biochemistry"},{"subject":"Space Science"},{"subject":"Cell Biology"},{"subject":"Molecular Biology"},{"subject":"Physiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biotechnology"},{"subject":"39999 Chemical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Chemical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Chemical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Hematology"}],"contributors":[],"dates":[{"date":"2016-12-14","dateType":"Created"},{"date":"2016-12-14","dateType":"Updated"},{"date":"2016","dateType":"Issued"}],"language":null,"types":{"ris":"RPRT","bibtex":"article","citeproc":"article-journal","schemaOrg":"ScholarlyArticle","resourceType":"Paper","resourceTypeGeneral":"Text"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1186/s40824-016-0066-2","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["3033166 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"CC BY + CC0","rightsUri":"https://creativecommons.org/licenses/by/4.0"}],"descriptions":[{"description":"Selection and conformation of lentivial transfected mouse stromal cells. (A) Flow cytometric analysis of GFP mBMSC, (B) Culture-expanded genetically engineered mBMSCs. (Scale bar, 200Îźm). Figure S2. Characterized secretion of human cytokines from genetically engineered stromal cells in 1 and 3 weeks in vitro culture. Figure S3. hSDF1a ELISA in mouse blood serum. Control mice without scaffold implantation showed a background level of SDF1a signal due to cross-reactivity. This level was used as a baseline and was also observed in growth arrested, which was concluded, as undetectable. The other groups showed measurable levels above background and were concluded to be true hSDF-1a detection. Figure S4. SEM images of growth-competent genetically engineered stromal cell-seeded scaffolds. (A) Cross-sectional images of human soluble factor secreting engineered stromal cell-seeded scaffolds after 6 weeks subcutaneous implantation. Except hTNFa, entire pores were completely filled with tissue cells with no hematopoietic components. (B) Closed-up image of growing engineered stromal cell-seeded scaffolds. Figure S5. Examples of semi-quantitative image analysis using ImageJ. (A) Collagen fiber area estimation from a Massonâ  s Trichrome staining image, (B) Vasculature area estimation from an immunohistostaining mCD31 and DAPI image. Figure S6. Long-term maintenance of inflammation-mimicking tissue microenvironment indirectly indicates survival and function of growth-arrested hTNFa secreting engineered stromal cells in the implanted scaffolds. 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