{"data":{"id":"10.5061/dryad.zs7h44jgw","type":"dois","attributes":{"doi":"10.5061/dryad.zs7h44jgw","prefix":"10.5061","suffix":"dryad.zs7h44jgw","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Wang, Wang","nameType":"Personal","givenName":"Wang","familyName":"Wang","affiliation":["University of Washington"],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0000-0001-9093-412X","nameIdentifierScheme":"ORCID"}]}],"titles":[{"title":"Increased Drp1 acetylation by lipid overload induces cardiomyocyte death and heart dysfunction"}],"publisher":"Dryad","container":{},"publicationYear":2024,"subjects":[{"subject":"FOS: Basic medicine","schemeUri":"https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf","subjectScheme":"fos"},{"subject":"FOS: Basic medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cardiovascular research"},{"subject":"Mitochondria","schemeUri":"https://github.com/PLOS/plos-thesaurus","subjectScheme":"PLOS Subject Area Thesaurus"},{"subject":"Metabolism","schemeUri":"https://github.com/PLOS/plos-thesaurus","subjectScheme":"PLOS Subject Area Thesaurus"},{"subject":"Metabolic disease"},{"subject":"high fat diet"},{"subject":"Dynamin related protein 1"}],"contributors":[],"dates":[{"date":"2023-12-28T23:06:10Z","dateType":"Created"},{"date":"2023-12-28T23:06:57Z","dateType":"Submitted"},{"date":"2024-01-16T00:00:00Z","dateType":"Issued"},{"date":"2024-01-16T00:00:00Z","dateType":"Available"}],"language":"en","types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsCitedBy","relatedIdentifier":"10.1161/circresaha.119.315252","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["8995726 bytes"],"formats":[],"version":"5","rightsList":[{"rights":"Creative Commons Zero v1.0 Universal","rightsUri":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc0-1.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Metabolic syndrome is a cluster of abnormalities characterized by obesity\n and insulin resistance, which compromise energy metabolism, damage\n mitochondria, cause cardiomyocyte death, and eventually impair heart\n contraction and relaxation performance. Despite the increasing prevalence\n of heart complications in obese and diabetic patients, our knowledge on\n how obese and diabetes mellitus impair heart function is very limited. In\n this study, we used animal and cell culture models in rodents or monkeys\n and generated lipid overload models to mimic obesity conditions. We found\n that excessive lipid supply decreased nicotinamide adenine dinucleotide\n (oxidized) levels and increased the acetylation of a fission protein Drp1\n at a specific lysine residue (K642). Drp1 acetylation at K642\n activated Drp1 through phosphorylation, mitochondrial translocation, and\n oligomerization. The excessively activated Drp1 had higher GTPase\n activity, bound with VDAC1 on mitochondria, induced mitochondrial fission,\n and caused cardiomyocyte death. These findings provide new information\n regarding how lipid overload regulates redox environment, protein\n acetylation, and the function of mitochondrial fission protein Drp1 in the\n heart.","descriptionType":"Abstract"},{"description":"1. All source data and raw Western Blotting (WB) images\n corresponding to the research paper 'Increased Drp1 Acetylation by\n Lipid Overload Induces Cardiomyocyte Death and Heart Dysfunction’\n published in Circulation Research. Methods are described in detail in the\n online supplemental materials of this paper. 2. The\n Excel files contain source data for the bar graphs of Figures 1–8, Online\n Figures I–VIII and Online Tables 1–2 of this paper. For clarity, each\n spreadsheet is labeled with its location (figure/panel) in the paper. The\n first cell in each spreadsheet contains a brief description of the\n contents. 3. The pdf file named: Raw WB images for Fig\n 1–8 S2 S6 \u0026amp; S7.pdf contains all the raw WB images in this paper,\n including images in Figures 1-8 and Online Figures II, VI, and VII. In\n some images, red rectangles are used to highlight the portion of the image\n used in the paper.","descriptionType":"Methods"},{"description":"This README file was generated on 2023-01-10 by Wang Wang. I. GENERAL\n INFORMATION 1. Title of dataset: Increased Drp1 Acetylation by Lipid\n Overload Induces Cardiomyocyte Death and Heart Dysfunction 2. Access this\n dataset on dryad: 3. Abstract:\n Metabolic syndrome is a cluster of abnormalities characterized by obesity\n and insulin resistance, which compromise energy metabolism, damage\n mitochondria, cause cardiomyocyte death, and eventually impair heart\n contraction and relaxation performance. Despite the increasing prevalence\n of heart complications in obese and diabetic patients, our knowledge on\n how obese and diabetes mellitus impair heart function is very limited. In\n this study, we used animal and cell culture models in rodents or monkeys\n and generated lipid overload models to mimic obesity conditions. We found\n that excessive lipid supply decreased nicotinamide adenine dinucleotide\n (oxidized) levels and increased the acetylation of a fission protein Drp1\n at a specific lysine residue (K642). Drp1 acetylation at K642 activated\n Drp1 through phosphorylation, mitochondrial translocation, and\n oligomerization. The excessively activated Drp1 had higher GTPase\n activity, bound with VDAC1 on mitochondria, induced mitochondrial fission,\n and caused cardiomyocyte death. These findings provide new information\n regarding how lipid overload regulates redox environment, protein\n acetylation, and the function of mitochondrial fission protein Drp1 in the\n heart. 4. Principal investigator contact information: Wang Wang,\n Department of Anesthesiology and Pain Medicine, University of Washington,\n Seattle WA USA. Email: 5. Date of data collection:\n 2018-2020 6. Geographic location of data collection: University of\n Washington, Seattle, WA USA 7. Funding sources that supported the\n collection of the data: (1) National Institutes of Health awards:\n HL114760, HL110349, HL137266, HL093671 and HL142864 (2) American Heart\n Association awards: 18EIA33900041 and 19CDA34660311 (3) National Institute\n on Aging (NIA) Intramural Research Program 8. Dataset contents (1) Figure\n 1 source data.xlsx (2) Figure 2 source data.xlsx (3) Figure 3 source\n data.xlsx (4) Figure 4 source data.xlsx (5) Figure 5 source data.xlsx (6)\n Figure 6 source data.xlsx (7) Figure 7 source data.xlsx (8) Figure 8\n source data.xlsx (9) Online Figure I source data.xlsx (10) Online Figure\n II source data.xlsx (11) Online Figure III source data.xlsx (12) Online\n Figure IV source data.xlsx (13) Online Figure V source data.xlsx (14)\n Online Figure VI source data.xlsx (15) Online Figure VII source data.xlsx\n (16) Online Figure VIII source data.xlsx (17) Online Tables 1-2 source\n data.xlsx (18) Raw WB images for Fig 1-8 S2 S6 \u0026amp; S7.pdf II.\n DESCRIPTION OF THE DATA AND FILE STRUCTURE 1. All source data and raw\n Western Blotting (WB) images corresponding to research paper\n \"Increased Drp1 Acetylation by Lipid Overload Induces Cardiomyocyte\n Death and Heart Dysfunction\" published in Circulation Research.\n Methods are described in detail in the online supplemental materials of\n this paper. 2. The Excel files contain source data for the bar graphs of\n Figures 1-8, Online Figures I-VIII and Online Tables 1-2 of this paper.\n For clarity, each spreadsheet is labeled with its location (figure/panel)\n in the paper. The first cell in each spreadsheet contains a briefly\n description of the contents. 3. The pdf file named: Raw WB images for Fig\n 1-8 S2 S6 \u0026amp; S7.pdf contains all the raw WB images in this paper,\n including images in Figures 1-8 and Online Figures II, VI, and VII. In\n some images, red rectangles are used to highlight the portion of the image\n used in the paper. III. SHARING/ACCESS INFORMATION 1. Links to other\n publicly accessible locations of the data: Hu Q, Zhang H, Gutierrez Cortes\n N, Wu D, Wang P, Zhang J, Mattison JA, Smith E, Bettcher LF, Wang M,\n Lakatta EG, Sheu SS and Wang W. Increased Drp1 Acetylation by Lipid\n Overload Induces Cardiomyocyte Death and Heart Dysfunction. Circulation\n research. 2020;126:456-470.\n 2. Recommended\n citation for this dataset: Wang Wang (2024). Data from: Increased Drp1\n Acetylation by Lipid Overload Induces Cardiomyocyte Death and Heart\n Dysfunction. Dryad Digital Repository.","descriptionType":"TechnicalInfo"}],"geoLocations":[],"fundingReferences":[{"schemeUri":"https://ror.org","funderName":"American Heart Association","awardNumber":"18EIA33900041","funderIdentifier":"https://ror.org/013kjyp64","funderIdentifierType":"ROR"},{"schemeUri":"https://ror.org","funderName":"National Heart, Lung, and Blood Institute","awardNumber":"HL114760","funderIdentifier":"https://ror.org/012pb6c26","funderIdentifierType":"ROR"},{"schemeUri":"https://ror.org","funderName":"American Heart Association","awardNumber":"19CDA34660311","funderIdentifier":"https://ror.org/013kjyp64","funderIdentifierType":"ROR"},{"schemeUri":"https://ror.org","funderName":"National Heart, Lung, and Blood Institute","awardNumber":"HL110349","funderIdentifier":"https://ror.org/012pb6c26","funderIdentifierType":"ROR"},{"schemeUri":"https://ror.org","funderName":"National Heart, Lung, and Blood Institute","awardNumber":"HL137266","funderIdentifier":"https://ror.org/012pb6c26","funderIdentifierType":"ROR"},{"schemeUri":"https://ror.org","funderName":"National Heart, Lung, and Blood Institute","awardNumber":"HL093671","funderIdentifier":"https://ror.org/012pb6c26","funderIdentifierType":"ROR"},{"schemeUri":"https://ror.org","funderName":"National Heart, Lung, and Blood Institute","awardNumber":"HL142864","funderIdentifier":"https://ror.org/012pb6c26","funderIdentifierType":"ROR"}],"xml":"<?xml version="1.0" encoding="UTF-8"?>
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      <creatorName nameType="Personal">Wang, Wang</creatorName>
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    </creator>
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  <titles>
    <title>
      Increased Drp1 acetylation by lipid overload induces cardiomyocyte death
      and heart dysfunction
    </title>
  </titles>
  <publisher publisherIdentifier="https://ror.org/00x6h5n95" publisherIdentifierScheme="ROR" schemeURI="https://ror.org/">Dryad</publisher>
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    <subject subjectScheme="fos" schemeURI="https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf">FOS: Basic medicine</subject>
    <subject>Cardiovascular research</subject>
    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Mitochondria</subject>
    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Metabolism</subject>
    <subject>Metabolic disease</subject>
    <subject>high fat diet</subject>
    <subject>Dynamin related protein 1</subject>
  </subjects>
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      <funderName>American Heart Association</funderName>
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      <funderName>American Heart Association</funderName>
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      <funderName>National Heart, Lung, and Blood Institute</funderName>
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      <awardNumber>HL110349</awardNumber>
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      <funderName>National Heart, Lung, and Blood Institute</funderName>
      <funderIdentifier funderIdentifierType="ROR">https://ror.org/012pb6c26</funderIdentifier>
      <awardNumber>HL142864</awardNumber>
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  <dates>
    <date dateType="Created">2023-12-28T23:06:10Z</date>
    <date dateType="Submitted">2023-12-28T23:06:57Z</date>
    <date dateType="Issued">2024-01-16T00:00:00Z</date>
    <date dateType="Available">2024-01-16T00:00:00Z</date>
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  <descriptions>
    <description descriptionType="Abstract">
      Metabolic syndrome is a cluster of abnormalities characterized by obesity
      and insulin resistance, which compromise energy metabolism, damage
      mitochondria, cause cardiomyocyte death, and eventually impair heart
      contraction and relaxation performance. Despite the increasing prevalence
      of heart complications in obese and diabetic patients, our knowledge on
      how obese and diabetes mellitus impair heart function is very limited. In
      this study, we used animal and cell culture models in rodents or monkeys
      and generated lipid overload models to mimic obesity conditions. We found
      that excessive lipid supply decreased nicotinamide adenine dinucleotide
      (oxidized) levels and increased the acetylation of a fission protein Drp1
      at a specific&amp;nbsp;lysine residue (K642). Drp1 acetylation at K642
      activated Drp1 through phosphorylation, mitochondrial translocation, and
      oligomerization. The excessively activated Drp1 had higher GTPase
      activity, bound with VDAC1 on mitochondria, induced mitochondrial fission,
      and caused cardiomyocyte death. These findings provide new information
      regarding how lipid overload regulates redox environment, protein
      acetylation, and the function of mitochondrial fission protein Drp1 in the
      heart.
    </description>
    <description descriptionType="Methods">
      &lt;p&gt;1. All source data and raw Western Blotting (WB) images
      corresponding to the research paper 'Increased Drp1 Acetylation by
      Lipid Overload Induces Cardiomyocyte Death and Heart Dysfunction’
      published in Circulation Research. Methods are described in detail in the
      online supplemental materials of this paper.&lt;/p&gt; &lt;p&gt;2. The
      Excel files contain source data for the bar graphs of Figures 1–8, Online
      Figures I–VIII and Online Tables 1–2 of this paper. For clarity, each
      spreadsheet is labeled with its location (figure/panel) in the paper. The
      first cell in each spreadsheet contains a brief description of the
      contents.&lt;/p&gt; &lt;p&gt;3. The pdf file named: Raw WB images for Fig
      1–8 S2 S6 &amp;amp; S7.pdf contains all the raw WB images in this paper,
      including images in Figures 1-8 and Online Figures II, VI, and VII. In
      some images, red rectangles are used to highlight the portion of the image
      used in the paper.&lt;/p&gt;
    </description>
    <description descriptionType="TechnicalInfo">
      This README file was generated on 2023-01-10 by Wang Wang. I. GENERAL
      INFORMATION 1. Title of dataset: Increased Drp1 Acetylation by Lipid
      Overload Induces Cardiomyocyte Death and Heart Dysfunction 2. Access this
      dataset on dryad: &lt;http://doi:10.5061/dryad.zs7h44jgw&gt; 3. Abstract:
      Metabolic syndrome is a cluster of abnormalities characterized by obesity
      and insulin resistance, which compromise energy metabolism, damage
      mitochondria, cause cardiomyocyte death, and eventually impair heart
      contraction and relaxation performance. Despite the increasing prevalence
      of heart complications in obese and diabetic patients, our knowledge on
      how obese and diabetes mellitus impair heart function is very limited. In
      this study, we used animal and cell culture models in rodents or monkeys
      and generated lipid overload models to mimic obesity conditions. We found
      that excessive lipid supply decreased nicotinamide adenine dinucleotide
      (oxidized) levels and increased the acetylation of a fission protein Drp1
      at a specific lysine residue (K642). Drp1 acetylation at K642 activated
      Drp1 through phosphorylation, mitochondrial translocation, and
      oligomerization. The excessively activated Drp1 had higher GTPase
      activity, bound with VDAC1 on mitochondria, induced mitochondrial fission,
      and caused cardiomyocyte death. These findings provide new information
      regarding how lipid overload regulates redox environment, protein
      acetylation, and the function of mitochondrial fission protein Drp1 in the
      heart. 4. Principal investigator contact information: Wang Wang,
      Department of Anesthesiology and Pain Medicine, University of Washington,
      Seattle WA USA. Email: &lt;wangwang@uw.edu&gt; 5. Date of data collection:
      2018-2020 6. Geographic location of data collection: University of
      Washington, Seattle, WA USA 7. Funding sources that supported the
      collection of the data: (1) National Institutes of Health awards:
      HL114760, HL110349, HL137266, HL093671 and HL142864 (2) American Heart
      Association awards: 18EIA33900041 and 19CDA34660311 (3) National Institute
      on Aging (NIA) Intramural Research Program 8. Dataset contents (1) Figure
      1 source data.xlsx (2) Figure 2 source data.xlsx (3) Figure 3 source
      data.xlsx (4) Figure 4 source data.xlsx (5) Figure 5 source data.xlsx (6)
      Figure 6 source data.xlsx (7) Figure 7 source data.xlsx (8) Figure 8
      source data.xlsx (9) Online Figure I source data.xlsx (10) Online Figure
      II source data.xlsx (11) Online Figure III source data.xlsx (12) Online
      Figure IV source data.xlsx (13) Online Figure V source data.xlsx (14)
      Online Figure VI source data.xlsx (15) Online Figure VII source data.xlsx
      (16) Online Figure VIII source data.xlsx (17) Online Tables 1-2 source
      data.xlsx (18) Raw WB images for Fig 1-8 S2 S6 &amp; S7.pdf II.
      DESCRIPTION OF THE DATA AND FILE STRUCTURE 1. All source data and raw
      Western Blotting (WB) images corresponding to research paper
      "Increased Drp1 Acetylation by Lipid Overload Induces Cardiomyocyte
      Death and Heart Dysfunction" published in Circulation Research.
      Methods are described in detail in the online supplemental materials of
      this paper. 2. The Excel files contain source data for the bar graphs of
      Figures 1-8, Online Figures I-VIII and Online Tables 1-2 of this paper.
      For clarity, each spreadsheet is labeled with its location (figure/panel)
      in the paper. The first cell in each spreadsheet contains a briefly
      description of the contents. 3. The pdf file named: Raw WB images for Fig
      1-8 S2 S6 &amp; S7.pdf contains all the raw WB images in this paper,
      including images in Figures 1-8 and Online Figures II, VI, and VII. In
      some images, red rectangles are used to highlight the portion of the image
      used in the paper. III. SHARING/ACCESS INFORMATION 1. Links to other
      publicly accessible locations of the data: Hu Q, Zhang H, Gutierrez Cortes
      N, Wu D, Wang P, Zhang J, Mattison JA, Smith E, Bettcher LF, Wang M,
      Lakatta EG, Sheu SS and Wang W. Increased Drp1 Acetylation by Lipid
      Overload Induces Cardiomyocyte Death and Heart Dysfunction. Circulation
      research. 2020;126:456-470.
      &lt;https://doi.org/10.1161/CIRCRESAHA.119.315252&gt; 2. Recommended
      citation for this dataset: Wang Wang (2024). Data from: Increased Drp1
      Acetylation by Lipid Overload Induces Cardiomyocyte Death and Heart
      Dysfunction. Dryad Digital Repository.
      &lt;https://doi:10.5061/dryad.zs7h44jgw&gt;
    </description>
  </descriptions>
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