{"data":{"id":"10.5061/dryad.zkh1893qf","type":"dois","attributes":{"doi":"10.5061/dryad.zkh1893qf","prefix":"10.5061","suffix":"dryad.zkh1893qf","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Straehla, Joelle","nameType":"Personal","givenName":"Joelle","familyName":"Straehla","affiliation":["Seattle Children's Hospital","University of Washington School of Medicine","Koch Institute for Integrative Cancer Research At MIT"],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0000-0002-9446-1718","nameIdentifierScheme":"ORCID"}]},{"name":"Nelson, Elizabeth","nameType":"Personal","givenName":"Elizabeth","familyName":"Nelson","affiliation":["Koch Institute for Integrative Cancer Research At MIT"],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0000-0002-2463-576X","nameIdentifierScheme":"ORCID"}]}],"titles":[{"title":"Image repository from : Subcellular nanoparticle trafficking investigated with label-free, live cell imaging"}],"publisher":"Dryad","container":{},"publicationYear":2026,"subjects":[{"subject":"FOS: Nano-technology","schemeUri":"https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf","subjectScheme":"fos"},{"subject":"Holographic microscopy","schemeUri":"https://github.com/PLOS/plos-thesaurus","subjectScheme":"PLOS Subject Area Thesaurus"},{"subject":"Fluorescence microscopy","schemeUri":"https://github.com/PLOS/plos-thesaurus","subjectScheme":"PLOS Subject Area Thesaurus"},{"subject":"Nanoparticles","schemeUri":"https://github.com/PLOS/plos-thesaurus","subjectScheme":"PLOS Subject Area Thesaurus"}],"contributors":[],"dates":[{"date":"2025-11-11T19:44:53Z","dateType":"Created"},{"date":"2026-02-27T05:01:53Z","dateType":"Submitted"},{"date":"2026-04-14T00:00:00Z","dateType":"Issued"},{"date":"2026-04-14T00:00:00Z","dateType":"Available"}],"language":"en","types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsCitedBy","relatedIdentifier":"10.1039/d5nh00749f","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["7835370897 bytes"],"formats":[],"version":"4","rightsList":[{"rights":"Creative Commons Zero v1.0 Universal","rightsUri":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc0-1.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Nanoparticle drug delivery systems have significant potential to transform\n precision medicine due to their ability to encapsulate a wide range of\n cargos that can be packaged in various types of carriers. Delivery\n kinetics can also be modified by altering the surface chemistry of the\n nanoparticle carrier. However, a gap remains in our understanding of the\n cellular mechanisms underlying nanoparticle uptake and subcellular\n trafficking kinetics. A comprehensive understanding of nanoparticle-cell\n interactions considering both the nanocarrier and the drug cargo has been\n challenging, partly due to technological limitations. Here, we present a\n robust long-term imaging workflow as a novel tool to study live-cell\n dynamics and nanoparticle delivery. We show how the integration of\n holography, tomography, and fluorescence microscopy enhances the capacity\n to study living, adherent cells in vitro label-free with further\n applications in assessing nanoparticle uptake and delivery kinetics over\n time. We also introduce a method of characterizing kinetic data from a\n library of lipid- and polymer-based nanoformulations without the need for\n cellular labels. This application of dynamic fluorescent holotomography as\n a new method to investigate nanoparticle uptake and cargo delivery\n highlights the expanding utility of multimodal, label-free, live imaging\n techniques.","descriptionType":"Abstract"},{"description":"# Image repository from : Subcellular nanoparticle trafficking\n investigated with label-free, live cell imaging Dataset DOI:\n [10.5061/dryad.zkh1893qf](https://doi.org/10.5061/dryad.zkh1893qf) ##\n Description of the data and file structure The contents of this Dryad\n dataset include all the acquisition files, edited montages generated using\n ImageJ, auto-adjusted processed acquisitions with added scale bars, and\n the metadata cell sheet, which describes the acquisition parameters set\n for the corresponding data file. \n \n The files are all TIFF files. These data are titled to indicate as much\n detail as necessary to identify the file. This includes: the format of the\n image (movie, still, Combined Stack, Merged, or montage), the cell line\n type (SKNBE2, T47D, or LOXIMVI as “LOX”), the treatment type (bare\n liposomes, LNPs, UT=Untreated, Doxil, or free dox[orubicin]), the\n durations (in hours) and/or what the scale bar is in microns (“um”).\n Further information in the titles may include the trial number (i.e. “001”\n or “002”) or the cell line delineation, which for LOXIMVI are Parental,\n Control LUC OE, or SLC OE; for T47D are Control or SLC KO; and for SKNBE2\n are Parental or PRRX1. For acquisitions performed in a 96-well plate,\n there are specific grid numbers included in the data title that\n arbitrarily indicate the grid number of interest for that image.\n Similarly, the slide number is identified in some data. Lastly, some\n titles include the observation of interest, such as “mitosis,” “death,” or\n “macro,” for macropinocytosis. Data is not available for reuse without\n permission from the corresponding author. \n \n The raw data is processed immediately after acquisition using the\n Nanolive SA proprietary feature, STEVE. In this code, the 96-stacks are\n combined per slide (between 50-350 slides per acquisition). This file is\n then used to process the maximum-intensity projection in ImageJ. All\n post-processing is automatically saved in stages to be able to view any\n slide or stack of choice throughout the acquisition. These data are not\n available in Dryad due to the large file sizes. All images were processed\n using ImageJ (aka FIJI). \n \n MICROSCOPY: Cells were imaged in a humidified, temperature-controlled\n chamber incubator (Tokai Hit) maintained at 37C with 5% CO~2~ supply.\n Temperature stabilization for 10-30 minutes is necessary to avoid fog\n interference before acquiring images. All images and movies were acquired\n on the 3D Cell Explorer 96focus microscope (Nanolive SA, Switzerland).\n Acquisitions using fluorescence were captured with filters for FITC or\n Cy5. Detailed acquisition parameters and experimental conditions can be\n found in the table below. \n \n ### Files and variables #### File: 2.5um-LOX-20um-every40slidse.tif\n **Description:** #### File: 250um-LOX-20um-every40slidse.tif\n **Description:** #### File: 250um-LOX-20um-every60slides.tif\n **Description:** #### File: 2.5um-LOX-20um-every60slides.tif\n **Description:** #### File: cannibalism-T47D-SLCKO-20um.tif\n **Description:** #### File: HA-LbL-Parental-mixed-dead-20um-scale.tif\n **Description:** #### File: HA-LbL-Parental-dead-20um.tif **Description:**\n #### File: LOX-freeDox-001-death-6xMontage-20um-bendta.tif\n **Description:** #### File:\n Group_1_wellA1_RI_MIP_FITC_Cy5_1_52_8bit_stitched-1.tif **Description:**\n #### File: HA-LbL-Parental-very-dead-20um.tif **Description:** #### File:\n LOX-freeDox-grid9-death-6xMontage-20um-timestamp.tif **Description:** ####\n File: LOX-freeDox-grid8-death-5xMontage-20um-over20hrs.tif\n **Description:** #### File: FreeDox-LOX-cells-moving-20um-bendta.tif\n **Description:** #### File: LOX-Parental-FreeDoxo-20um-deadcells.tif\n **Description:** #### File:\n LOX-LUC-OE-LNP001-photobleach-20um-RI-Cy5-only.tif **Description:** ####\n File: LUC_OE_3D_cy5_t_24_002.tif **Description:** #### File:\n LUC_OE_mitosis_10um.tif **Description:** #### File:\n LOX-SLC-OE-LNP002-Crop-20um-8fps.tif **Description:** #### File:\n LUC_OE_wellD3_RI_20um_edit.tif **Description:** #### File:\n LOX-LUC-OE-LNP001-photobleach-20um.tif **Description:** #### File:\n HA-LbL-Doxil-RI-25hours-20um-crop.tif **Description:** #### File:\n HA_LbLDoxil_RImovie_hours_25um.tif **Description:** #### File:\n macropino-Montage-1.tif **Description:** #### File:\n LUC-OE_UT_mitosis_and_death_cropped_10um.tif **Description:** #### File:\n macropino-Montage-2.tif **Description:** #### File:\n LUC-OE_UT_mitosis_and_death_cropped.tif **Description:** #### File:\n HA-LbL-Doxil-RI-hours-20um-movie.tif **Description:** #### File:\n merge_A1_LOX_PS_bare_20um.tif **Description:** #### File:\n Mitosis-LUC-OE-10um-Montage.tif **Description:** #### File:\n LOX-Parental-Doxil-002-grid3-20um-dyingcells-timestamp.tif\n **Description:** #### File:\n LOX-Parental-Doxil-002-grid3-20um-dyingcells.tif **Description:** ####\n File: Montage-6x-DOX002-FreeDox.tif **Description:** #### File:\n Montage-6x-DOX002-Doxil.tif **Description:** #### File:\n LOX-Parental-Doxil-002-grid9-20um-dyingcells-timestamp.tif\n **Description:** #### File: Montage4-T47D-LNP-20um.tif **Description:**\n #### File: Montage-6x-DOX002-Untreated.tif **Description:** #### File:\n Montage2-T47D-LNP-20um.tif **Description:** #### File:\n Montage3-T47D-LNP-20um.tif **Description:** #### File:\n Montage6-T47D-SLCKO-LNP-20um.tif **Description:** #### File:\n Montage5-T47D-SLCKO-LNP-20um.tif **Description:** #### File:\n Montage8-T47D-SLCKO-LNP-20um.tif **Description:** #### File:\n Montage-6x-DOX002-HA.tif **Description:** #### File:\n PS-bare-merge-Group_1_wellA1_RI_MIP_FITC_Cy5_1_52_8bit_stitched-1-1.tif\n **Description:** #### File: SKNBE2-PRRX1-cell-10um.tif **Description:**\n #### File: Montage1-T47D-LNP-20um.tif **Description:** #### File:\n Montage7-T47D-SLCKO-LNP-20um.tif **Description:** #### File: new_stack.tif\n **Description:** #### File: old-bare-lipo-control-24hr-merge.tif\n **Description:** #### File:\n LOX-Parental-Doxil-002-grid8-20um-dyingcells.tif **Description:** ####\n File: SLC_KO_F5_wellG8_RI_20um_edit.tif **Description:** #### File:\n old-bare-lipo-control-24hr.tif **Description:** #### File:\n SLC_OE_wellB8_RI_20um.tif **Description:** #### File:\n SLC_OE_wellB8_RI_20um_edit.tif **Description:** #### File:\n Montage-6x-DOX002-PLE.tif **Description:** #### File:\n LOX-Parental-Doxil-002-FreeDoxo-20um.tif **Description:** #### File:\n VG-Dend-PolyIC-NP-macs-10um.tif **Description:** #### File:\n WT_wellF3_RI_20um.tif **Description:** #### File:\n WT_wellF3_RI_20um_edit.tif **Description:** #### File:\n T47D-SLCKO-F5-macropino-magenta-20um.tif **Description:** #### File:\n SLC-OE-LNP-002-after-5hr.tif **Description:** #### File:\n SKNBE2-PRRX1-mitosis-8fps-10um.tif **Description:** #### File:\n vyxeos-250um-slide16.tif **Description:** #### File: UT-slide16.tif\n **Description:** #### File: SLC-OE-UT-cells-20um.tif **Description:** ####\n File: UT-slide199.tif **Description:** #### File:\n vyxeos-250um-16hr-CombinedStacks.tif **Description:** #### File:\n vyxeos-250um-slide199.tif **Description:** #### File:\n vyxeos-16hr-UTvs250um-Combined_Stacks.tif **Description:** #### File:\n vyxeos-UT-16hr-CombinedStacks.tif **Description:** #### File:\n T47D-SLCKO-LNP002-merge-macro-8fps-20um-11hours.tif **Description:** ####\n File: T47D-SLCKO-LNP002-merge-macro-8fps-20um.tif **Description:** ####\n File: T47D-SLCKO-Merged-macropino-20um.tif **Description:** \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | | :------ | :----------------------------------------- | :----------- |\n :------------------ | :-------- | :------------- | :--------------- |\n :----- | :------------ | :----- | :----- | :------------ | :----- |\n :------------------------------------ | | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | CY5 | FITC | \n \n | \n \n | \n \n | \n \n | \n \n | | Date | Experiment NP | Cell type(s) | Total duration (hr) | Grid scan\n | RI every X min | Fluo every X min | Power | Exposure (ms) | Gain | Power\n | Exposure (ms) | Gain | EVE analysis performed | | 4/30/24 | Cy5 LNPs\n containing GFP mRNA | LOX IMVI | 20 | 4x4 | 8 | 8 | 12% | 80 | 50 | 8% |\n 75 | 30 | Cytotoxicity assay | | \n \n | \n \n | T47D | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | | 5/9/24 | Carboxylated PS NPs and Cy5 bare liposomes | LOX IMVI | 16 |\n 1x1 | 12 | 12 | 10% | 80 | 50 | 8% | 50 | 15 | Cytotoxicity assay | | \n \n | \n \n | T47D | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | | 5/22/24 | UT and Cy5 LNPs containing GFP mRNA | LOX IMVI | 24 | 4x4 |\n 7 | 7 | 10% | 100 | 30 | 12% | 150 | 35 | Cytotoxicity and Lipid droplet\n assays | | 6/25/24 | Doxorubicin-loaded NPs | LOX IMVI | 35 | 3x3 | 5 | -\n | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | | 6/27/24 | CPX-351 (VYXEOS) | LOX IMVI | 30 | 3x3 | 5 | - | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | \n \n | | 5/15/24 | Free PIC (Cy5) and PIC NPs (FITC) | DCs | 5 | 3x3 | 10 | 10\n | 15% | 100 | 40 | 10% | 100 | 40 | \n \n | | 6/5/24 | first 3 hours | DCs | 3 | 4x4 | 6 | 6 | 15% | 100 | 30 | 5%\n | 75 | 25 | \n \n | | 6/5/24 | 20 hours | DCs | 18 | 4x4 | 8 | 8 | 15% | 120 | 30 | 7% | 75\n | 25 | \n \n | ## Code/software IMAGE ANALYSIS AND FIJI PROCESSING: Grid scan\n stitching was performed automatically using Nanolive SA proprietary\n analysis software (EVE). Automated analysis programs (LIVE Cytotoxicity\n Assay, LIVE Cell Death Assay, or the SMART Lipid Droplet Assay ^LIVE^ (all\n Nanolive SA)) were applied to the raw imaging data for automated\n segmentation and quantification of cells and lipid droplets, and for\n automated classification of cells into living, apoptotic, or necrotic\n states. All movies with fluorescent channels were processed in FIJI using\n Combine Stacks. For visualization after analysis, fluorescence and\n brightfield refractive index images were auto-adjusted for brightness and\n contrast. The raw data is processed immediately after acquisition using\n the Nanolive SA proprietary feature, STEVE. In this code, the 96-stacks\n are combined per slide (between 50-350 slides per acquisition). This file\n is then used to process the maximum-intensity projection in ImageJ. All\n post-processing is automatically saved in stages to be able to view any\n slide or stack of choice throughout the acquisition. These data are not\n available in Dryad due to the large file sizes. All images were processed\n using ImageJ (aka FIJI).  ## Access information Other publicly accessible\n locations of the data: * NA Data was derived from the following sources: *\n NA","descriptionType":"TechnicalInfo"}],"geoLocations":[],"fundingReferences":[],"xml":"<?xml version="1.0" encoding="UTF-8"?>
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  <identifier identifierType="DOI">10.5061/DRYAD.ZKH1893QF</identifier>
  <creators>
    <creator>
      <creatorName nameType="Personal">Straehla, Joelle</creatorName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0002-9446-1718</nameIdentifier>
      <affiliation affiliationIdentifier="https://ror.org/01njes783" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Seattle Children's Hospital</affiliation>
      <affiliation affiliationIdentifier="https://ror.org/03ymk3103" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of Washington School of Medicine</affiliation>
      <affiliation affiliationIdentifier="https://ror.org/01xd6q208" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Koch Institute for Integrative Cancer Research At MIT</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Nelson, Elizabeth</creatorName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0002-2463-576X</nameIdentifier>
      <affiliation affiliationIdentifier="https://ror.org/01xd6q208" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Koch Institute for Integrative Cancer Research At MIT</affiliation>
    </creator>
  </creators>
  <titles>
    <title>
      Image repository from : Subcellular nanoparticle trafficking investigated
      with label-free, live cell imaging
    </title>
  </titles>
  <publisher publisherIdentifier="https://ror.org/00x6h5n95" publisherIdentifierScheme="ROR" schemeURI="https://ror.org/">Dryad</publisher>
  <resourceType resourceTypeGeneral="Dataset">dataset</resourceType>
  <publicationYear>2026</publicationYear>
  <subjects>
    <subject subjectScheme="fos" schemeURI="https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf">FOS: Nano-technology</subject>
    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Holographic microscopy</subject>
    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Fluorescence microscopy</subject>
    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Nanoparticles</subject>
  </subjects>
  <dates>
    <date dateType="Created">2025-11-11T19:44:53Z</date>
    <date dateType="Submitted">2026-02-27T05:01:53Z</date>
    <date dateType="Issued">2026-04-14T00:00:00Z</date>
    <date dateType="Available">2026-04-14T00:00:00Z</date>
  </dates>
  <language>en</language>
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  <rightsList>
    <rights rightsURI="https://spdx.org/licenses/CC0-1.0.html">Creative Commons Zero v1.0 Universal</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">
      Nanoparticle drug delivery systems have significant potential to transform
      precision medicine due to their ability to encapsulate a wide range of
      cargos that can be packaged in various types of carriers. Delivery
      kinetics can also be modified by altering the surface chemistry of the
      nanoparticle carrier. However, a gap remains in our understanding of the
      cellular mechanisms underlying nanoparticle uptake and subcellular
      trafficking kinetics. A comprehensive understanding of nanoparticle-cell
      interactions considering both the nanocarrier and the drug cargo has been
      challenging, partly due to technological limitations. Here, we present a
      robust long-term imaging workflow as a novel tool to study live-cell
      dynamics and nanoparticle delivery. We show how the integration of
      holography, tomography, and fluorescence microscopy enhances the capacity
      to study living, adherent cells in vitro label-free with further
      applications in assessing nanoparticle uptake and delivery kinetics over
      time. We also introduce a method of characterizing kinetic data from a
      library of lipid- and polymer-based nanoformulations without the need for
      cellular labels. This application of dynamic fluorescent holotomography as
      a new method to investigate nanoparticle uptake and cargo delivery
      highlights the expanding utility of multimodal, label-free, live imaging
      techniques.
    </description>
    <description descriptionType="TechnicalInfo">
      # Image repository from : Subcellular nanoparticle trafficking
      investigated with label-free, live cell imaging Dataset DOI:
      [10.5061/dryad.zkh1893qf](https://doi.org/10.5061/dryad.zkh1893qf) ##
      Description of the data and file structure The contents of this Dryad
      dataset include all the acquisition files, edited montages generated using
      ImageJ, auto-adjusted processed acquisitions with added scale bars, and
      the metadata cell sheet, which describes the acquisition parameters set
      for the corresponding data file. 
      <br/>
       The files are all TIFF files. These data are titled to indicate as much
      detail as necessary to identify the file. This includes: the format of the
      image (movie, still, Combined Stack, Merged, or montage), the cell line
      type (SKNBE2, T47D, or LOXIMVI as “LOX”), the treatment type (bare
      liposomes, LNPs, UT=Untreated, Doxil, or free dox[orubicin]), the
      durations (in hours) and/or what the scale bar is in microns (“um”).
      Further information in the titles may include the trial number (i.e. “001”
      or “002”) or the cell line delineation, which for LOXIMVI are Parental,
      Control LUC OE, or SLC OE; for T47D are Control or SLC KO; and for SKNBE2
      are Parental or PRRX1. For acquisitions performed in a 96-well plate,
      there are specific grid numbers included in the data title that
      arbitrarily indicate the grid number of interest for that image.
      Similarly, the slide number is identified in some data. Lastly, some
      titles include the observation of interest, such as “mitosis,” “death,” or
      “macro,” for macropinocytosis. Data is not available for reuse without
      permission from the corresponding author. 
      <br/>
       The raw data is processed immediately after acquisition using the
      Nanolive SA proprietary feature, STEVE. In this code, the 96-stacks are
      combined per slide (between 50-350 slides per acquisition). This file is
      then used to process the maximum-intensity projection in ImageJ. All
      post-processing is automatically saved in stages to be able to view any
      slide or stack of choice throughout the acquisition. These data are not
      available in Dryad due to the large file sizes. All images were processed
      using ImageJ (aka FIJI). 
      <br/>
       MICROSCOPY: Cells were imaged in a humidified, temperature-controlled
      chamber incubator (Tokai Hit) maintained at 37C with 5% CO~2~ supply.
      Temperature stabilization for 10-30 minutes is necessary to avoid fog
      interference before acquiring images. All images and movies were acquired
      on the 3D Cell Explorer 96focus microscope (Nanolive SA, Switzerland).
      Acquisitions using fluorescence were captured with filters for FITC or
      Cy5. Detailed acquisition parameters and experimental conditions can be
      found in the table below. 
      <br/>
       ### Files and variables #### File: 2.5um-LOX-20um-every40slidse.tif
      **Description:** #### File: 250um-LOX-20um-every40slidse.tif
      **Description:** #### File: 250um-LOX-20um-every60slides.tif
      **Description:** #### File: 2.5um-LOX-20um-every60slides.tif
      **Description:** #### File: cannibalism-T47D-SLCKO-20um.tif
      **Description:** #### File: HA-LbL-Parental-mixed-dead-20um-scale.tif
      **Description:** #### File: HA-LbL-Parental-dead-20um.tif **Description:**
      #### File: LOX-freeDox-001-death-6xMontage-20um-bendta.tif
      **Description:** #### File:
      Group_1_wellA1_RI_MIP_FITC_Cy5_1_52_8bit_stitched-1.tif **Description:**
      #### File: HA-LbL-Parental-very-dead-20um.tif **Description:** #### File:
      LOX-freeDox-grid9-death-6xMontage-20um-timestamp.tif **Description:** ####
      File: LOX-freeDox-grid8-death-5xMontage-20um-over20hrs.tif
      **Description:** #### File: FreeDox-LOX-cells-moving-20um-bendta.tif
      **Description:** #### File: LOX-Parental-FreeDoxo-20um-deadcells.tif
      **Description:** #### File:
      LOX-LUC-OE-LNP001-photobleach-20um-RI-Cy5-only.tif **Description:** ####
      File: LUC_OE_3D_cy5_t_24_002.tif **Description:** #### File:
      LUC_OE_mitosis_10um.tif **Description:** #### File:
      LOX-SLC-OE-LNP002-Crop-20um-8fps.tif **Description:** #### File:
      LUC_OE_wellD3_RI_20um_edit.tif **Description:** #### File:
      LOX-LUC-OE-LNP001-photobleach-20um.tif **Description:** #### File:
      HA-LbL-Doxil-RI-25hours-20um-crop.tif **Description:** #### File:
      HA_LbLDoxil_RImovie_hours_25um.tif **Description:** #### File:
      macropino-Montage-1.tif **Description:** #### File:
      LUC-OE_UT_mitosis_and_death_cropped_10um.tif **Description:** #### File:
      macropino-Montage-2.tif **Description:** #### File:
      LUC-OE_UT_mitosis_and_death_cropped.tif **Description:** #### File:
      HA-LbL-Doxil-RI-hours-20um-movie.tif **Description:** #### File:
      merge_A1_LOX_PS_bare_20um.tif **Description:** #### File:
      Mitosis-LUC-OE-10um-Montage.tif **Description:** #### File:
      LOX-Parental-Doxil-002-grid3-20um-dyingcells-timestamp.tif
      **Description:** #### File:
      LOX-Parental-Doxil-002-grid3-20um-dyingcells.tif **Description:** ####
      File: Montage-6x-DOX002-FreeDox.tif **Description:** #### File:
      Montage-6x-DOX002-Doxil.tif **Description:** #### File:
      LOX-Parental-Doxil-002-grid9-20um-dyingcells-timestamp.tif
      **Description:** #### File: Montage4-T47D-LNP-20um.tif **Description:**
      #### File: Montage-6x-DOX002-Untreated.tif **Description:** #### File:
      Montage2-T47D-LNP-20um.tif **Description:** #### File:
      Montage3-T47D-LNP-20um.tif **Description:** #### File:
      Montage6-T47D-SLCKO-LNP-20um.tif **Description:** #### File:
      Montage5-T47D-SLCKO-LNP-20um.tif **Description:** #### File:
      Montage8-T47D-SLCKO-LNP-20um.tif **Description:** #### File:
      Montage-6x-DOX002-HA.tif **Description:** #### File:
      PS-bare-merge-Group_1_wellA1_RI_MIP_FITC_Cy5_1_52_8bit_stitched-1-1.tif
      **Description:** #### File: SKNBE2-PRRX1-cell-10um.tif **Description:**
      #### File: Montage1-T47D-LNP-20um.tif **Description:** #### File:
      Montage7-T47D-SLCKO-LNP-20um.tif **Description:** #### File: new_stack.tif
      **Description:** #### File: old-bare-lipo-control-24hr-merge.tif
      **Description:** #### File:
      LOX-Parental-Doxil-002-grid8-20um-dyingcells.tif **Description:** ####
      File: SLC_KO_F5_wellG8_RI_20um_edit.tif **Description:** #### File:
      old-bare-lipo-control-24hr.tif **Description:** #### File:
      SLC_OE_wellB8_RI_20um.tif **Description:** #### File:
      SLC_OE_wellB8_RI_20um_edit.tif **Description:** #### File:
      Montage-6x-DOX002-PLE.tif **Description:** #### File:
      LOX-Parental-Doxil-002-FreeDoxo-20um.tif **Description:** #### File:
      VG-Dend-PolyIC-NP-macs-10um.tif **Description:** #### File:
      WT_wellF3_RI_20um.tif **Description:** #### File:
      WT_wellF3_RI_20um_edit.tif **Description:** #### File:
      T47D-SLCKO-F5-macropino-magenta-20um.tif **Description:** #### File:
      SLC-OE-LNP-002-after-5hr.tif **Description:** #### File:
      SKNBE2-PRRX1-mitosis-8fps-10um.tif **Description:** #### File:
      vyxeos-250um-slide16.tif **Description:** #### File: UT-slide16.tif
      **Description:** #### File: SLC-OE-UT-cells-20um.tif **Description:** ####
      File: UT-slide199.tif **Description:** #### File:
      vyxeos-250um-16hr-CombinedStacks.tif **Description:** #### File:
      vyxeos-250um-slide199.tif **Description:** #### File:
      vyxeos-16hr-UTvs250um-Combined_Stacks.tif **Description:** #### File:
      vyxeos-UT-16hr-CombinedStacks.tif **Description:** #### File:
      T47D-SLCKO-LNP002-merge-macro-8fps-20um-11hours.tif **Description:** ####
      File: T47D-SLCKO-LNP002-merge-macro-8fps-20um.tif **Description:** ####
      File: T47D-SLCKO-Merged-macropino-20um.tif **Description:** 
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       | | :------ | :----------------------------------------- | :----------- |
      :------------------ | :-------- | :------------- | :--------------- |
      :----- | :------------ | :----- | :----- | :------------ | :----- |
      :------------------------------------ | | 
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       | CY5 | FITC | 
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       | | Date | Experiment NP | Cell type(s) | Total duration (hr) | Grid scan
      | RI every X min | Fluo every X min | Power | Exposure (ms) | Gain | Power
      | Exposure (ms) | Gain | EVE analysis performed | | 4/30/24 | Cy5 LNPs
      containing GFP mRNA | LOX IMVI | 20 | 4x4 | 8 | 8 | 12% | 80 | 50 | 8% |
      75 | 30 | Cytotoxicity assay | | 
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       | T47D | 
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       | | 5/9/24 | Carboxylated PS NPs and Cy5 bare liposomes | LOX IMVI | 16 |
      1x1 | 12 | 12 | 10% | 80 | 50 | 8% | 50 | 15 | Cytotoxicity assay | | 
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       | | 5/22/24 | UT and Cy5 LNPs containing GFP mRNA | LOX IMVI | 24 | 4x4 |
      7 | 7 | 10% | 100 | 30 | 12% | 150 | 35 | Cytotoxicity and Lipid droplet
      assays | | 6/25/24 | Doxorubicin-loaded NPs | LOX IMVI | 35 | 3x3 | 5 | -
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       | | 6/27/24 | CPX-351 (VYXEOS) | LOX IMVI | 30 | 3x3 | 5 | - | 
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       | | 5/15/24 | Free PIC (Cy5) and PIC NPs (FITC) | DCs | 5 | 3x3 | 10 | 10
      | 15% | 100 | 40 | 10% | 100 | 40 | 
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       | | 6/5/24 | first 3 hours | DCs | 3 | 4x4 | 6 | 6 | 15% | 100 | 30 | 5%
      | 75 | 25 | 
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       | | 6/5/24 | 20 hours | DCs | 18 | 4x4 | 8 | 8 | 15% | 120 | 30 | 7% | 75
      | 25 | 
      <br/>
       | ## Code/software IMAGE ANALYSIS AND FIJI PROCESSING: Grid scan
      stitching was performed automatically using Nanolive SA proprietary
      analysis software (EVE). Automated analysis programs (LIVE Cytotoxicity
      Assay, LIVE Cell Death Assay, or the SMART Lipid Droplet Assay ^LIVE^ (all
      Nanolive SA)) were applied to the raw imaging data for automated
      segmentation and quantification of cells and lipid droplets, and for
      automated classification of cells into living, apoptotic, or necrotic
      states. All movies with fluorescent channels were processed in FIJI using
      Combine Stacks. For visualization after analysis, fluorescence and
      brightfield refractive index images were auto-adjusted for brightness and
      contrast. The raw data is processed immediately after acquisition using
      the Nanolive SA proprietary feature, STEVE. In this code, the 96-stacks
      are combined per slide (between 50-350 slides per acquisition). This file
      is then used to process the maximum-intensity projection in ImageJ. All
      post-processing is automatically saved in stages to be able to view any
      slide or stack of choice throughout the acquisition. These data are not
      available in Dryad due to the large file sizes. All images were processed
      using ImageJ (aka FIJI).  ## Access information Other publicly accessible
      locations of the data: * NA Data was derived from the following sources: *
      NA
    </description>
  </descriptions>
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