{"data":{"id":"10.5061/dryad.z34tmpgq9","type":"dois","attributes":{"doi":"10.5061/dryad.z34tmpgq9","prefix":"10.5061","suffix":"dryad.z34tmpgq9","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Yang, Ying","nameType":"Personal","givenName":"Ying","familyName":"Yang","affiliation":["UNSW Sydney"],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0000-0001-6009-4748","nameIdentifierScheme":"ORCID"}]},{"name":"Ma, Yuanqing","nameType":"Personal","givenName":"Yuanqing","familyName":"Ma","affiliation":["UNSW Sydney"],"nameIdentifiers":[]},{"name":"Gooding, Justin","nameType":"Personal","givenName":"Justin","familyName":"Gooding","affiliation":["UNSW Sydney"],"nameIdentifiers":[]}],"titles":[{"title":"Electrochemically controlled switching of dyes for enhanced super-resolution optical fluctuation imaging (EC-SOFI)"}],"publisher":"Dryad","container":{},"publicationYear":2025,"subjects":[{"subject":"FOS: Nano-technology","schemeUri":"https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf","subjectScheme":"fos"},{"subject":"Optical microscopy","schemeUri":"https://github.com/PLOS/plos-thesaurus","subjectScheme":"PLOS Subject Area Thesaurus"},{"subject":"super-resolution optical fluctuation imaging"},{"subject":"Electrochemical switching of dyes"}],"contributors":[{"name":"UNSW Sydney","affiliation":[],"contributorType":"Sponsor","nameIdentifiers":[]}],"dates":[{"date":"2025-06-25T12:21:38Z","dateType":"Created"},{"date":"2025-06-25T12:25:52Z","dateType":"Submitted"},{"date":"2025-07-18T00:00:00Z","dateType":"Issued"},{"date":"2025-07-18T00:00:00Z","dateType":"Available"}],"language":"en","types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsCitedBy","relatedIdentifier":"10.1073/pnas.2425390122","relatedIdentifierType":"DOI"},{"relationType":"IsCitedBy","relatedIdentifier":"10.1101/2024.06.02.597055","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["5742285759 bytes"],"formats":[],"version":"5","rightsList":[{"rights":"Creative Commons Zero v1.0 Universal","rightsUri":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc0-1.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"In super-resolution optical fluctuation imaging (SOFI), molecules spaced\n closer than the diffraction limit can be identified through spatial and\n temporal correlation analysis of the fluorescence intensity fluctuations.\n The resolution and speed of SOFI imaging greatly depend on the\n characteristics of these fluorescence fluctuations. Fluorophores with\n stochastic and rapid fluorescence fluctuations are favourable for\n improving SOFI imaging resolution and speed, especially in high order\n cumulant analysis. Stochastic blinking dyes in specific buffers are\n well-suited for SOFI due to their high brightness and strong fluorescence\n fluctuations. However, their overall blinking rate can sometimes be too\n slow, presenting practical limitations for high-resolution and fast SOFI\n imaging. Furthermore, dye blinking can be non-uniform, creating\n nonlinearity in SOFI image. To address these challenges, we introduce\n electrochemically controlled dye switching for SOFI (EC-SOFI). By applying\n an oscillating electrochemical potential to a transparent electrode\n surface, we increase the overall dye switching rate and uniformity. Using\n Alexa 647 dye as an example, EC-SOFI reduces the average ON time by over\n 3-fold and switching variance by more than 2-fold compared to conventional\n photochemical switching, achieving ~60 nm spatial resolution in 6th order\n EC-SOFI image. We further demonstrate that EC-SOFI achieves ~130 nm and\n ~80 nm resolution with 100 and 300 frames, respectively, enabling fast,\n large-area tile-scan super-resolution imaging. This advancement in EC-SOFI\n significantly enhances the practical potential of SOFI technique.","descriptionType":"Abstract"},{"description":"\u003cstrong\u003eElectrochemistry:\u003c/strong\u003e\u003cbr\u003eElectrochemical measurements, including chronoamperometry and large-amplitude sinusoidal voltammetry, were performed using an SP-200 potentiostat (Bio-Logic, France) in a custom EC imaging chamber (Chamlide EC 22, Live Cell Instrument, Korea). The setup featured a three-electrode system: an ITO-coated coverslip (SPI Supplies, USA) as the working electrode, an Ag|AgCl|3M KCl reference electrode (LF-1-45, Innovative Instruments, USA), and a Pt mesh counter electrode. The chamber was mounted on the microscope stage for simultaneous EC control and fluorescence imaging. \u003cstrong\u003eImage acquisition:\u003c/strong\u003e\u003cbr\u003eSOFI and STORM images were acquired on a Zeiss Elyra 7 Lattice SIM TIRF microscope with a 642 nm laser and sCMOS camera (edge 4.2, PCO). Illumination (EPI or TIRF) was controlled by shifting focus laterally in the objective's back focal plane. A 1.6× OptoVar lens reduced pixel size to 100 nm. Fluorescence was collected by the same 100× 1.46 NA oil objective and detected by the cooled sCMOS camera.","descriptionType":"Methods"},{"description":"# Data for: Electrochemically controlled switching of dyes for enhanced\n super-resolution optical fluctuation imaging (EC-SOFI) **Dataset DOI:**\n 10.5061/dryad.z34tmpgq9\\ **Corresponding Author:** To whom correspondence\n may be addressed. Email:\n [yuanqing.ma@unsw.edu.au](mailto:yuanqing.ma@unsw.edu.au) or\n [justin.gooding@unsw.edu.au](mailto:justin.gooding@unsw.edu.au). **1.\n Included Files** This dataset contains the raw imaging data used to\n generate Figures 1-4 in the associated publication. All image data are\n provided wither as .csv or`.tif` files inside `.zip` archives, directly\n exported from Zeiss Zen Blue.\n **Fig_1_single_molecule_photophysics_using_origami.zip**\\ This folder\n includes the sinple molecule localization data in .csv format used for\n generating Figure 1, which shows single-molecule fluorescence trajectories\n using DNA origami labelled with Alexa 647. Four distinct regions of\n interest (ROI 1–4) are included for two experimental conditions: o   \n **Electrochemical (EC)**: 0.5 kW cm−2 642 nm laser were on, large\n amplitude square wave (LASV) potential was sinusoidally oscillated between\n −0.8 V to 0.1 V with a frequency of 10 Hz o    **UV Illumination**: 0.5 W\n cm−2 of UV laser and 0.5 kW cm−2 642 nm laser were on. These files of\n single-molecule localization results were directly exported from Zeiss Zen\n Blue, with each row corresponds to a detected fluorescent molecule. Below\n are the definitions of each column: * **Index**: Unique identifier for\n each localization event. * **First Frame**: Frame number when the molecule\n first appeared. * **Number Frames**: Total number of frames the molecule\n was detected. * **Frames Missing**: Number of skipped frames during a\n blinking event. * **X Position (nm)** / **Y Position (nm)**: Lateral\n coordinates of the molecule in nanometers. * **Precision (nm)**: Estimated\n localization uncertainty in X/Y, based on fitting. * **Photons**: Total\n photon count collected from the molecule. Reflects signal brightness. *\n **Background Variance**: Variability of background noise in the fitting\n region. * **Chi Square**: Goodness-of-fit indicator for the Gaussian\n model. * **PSF width (nm)**: Width of the fitted point spread function in\n nanometers. * **Channel**: Color or channel label used during imaging. *\n **Z-Slice**: Z-stack slice index (for 3D acquisitions). * **Z Position\n (nm)**: Axial (depth) position of the molecule in nanometers. * **Z\n Precision (nm)**: Estimated localization uncertainty in Z direction.\n **Fig_2_UV_vs_EC_SOFI.zip**\\ Contains raw SOFI image sequences used to\n generate Figure 2, comparing UV- and EC-SOFI in fixed COS-7 cells. The\n data highlight differences in switching uniformity and kinetics.\n **Fig_3_High_order_SOFI.zip**\\ Includes raw data used in high-order SOFI\n reconstruction shown in Figure 3. These sequences were used to calculate\n up to 6th-order SOFI images, demonstrating resolution enhancement using\n electrochemically modulated dye switching. **Fig_4_Tile_Scan.zip**\\\n Contains one example for raw tiled image stacks used in Figure 4, more\n similar tiles were collected to achieve wide-area EC-SOFI imaging. ## 2.\n Recommended Software The .tif files provided in this dataset can be opened\n using freely available software: **Fiji (ImageJ distribution)** –\n Recommended for most users; supports stack viewing, analysis, and basic\n SOFI processing.\\ Website:\n [https://imagej.net/software/fiji](https://imagej.net/software/fiji)\n **ImageJ** – A lightweight alternative to Fiji, with broad compatibility\n for viewing and simple operations.\\ Website:\n [https://imagej.nih.gov/ij/](https://imagej.nih.gov/ij/) No proprietary\n software or plugins are needed to access the data. ## 3. Imaging settings:\n ·       **Excitation wavelength**: For most experiments, a 640 nm laser\n was used; UV conditions refer to 400 nm laser illumination. ·      \n **Applied potential**: In EC conditions, potentials were alternating\n between –0.8 V and +0.15 V versus Ag/AgCl/3M KCl reference. Additional\n experimental parameters such as sample preparation, buffer composition,\n and detailed EC and imaging settings are available in the\n manuscript's Methods section. ## 4. Citation If you use this dataset,\n please cite: Y. Yang,Y. Ma,R.D. Tilley,\u0026amp; J.J. Gooding, \n Electrochemically controlled switching of dyes for enhanced\n superresolution optical fluctuation imaging (EC-SOFI), Proc. Natl. Acad.\n Sci. U.S.A. 122 (28) e2425390122,\n [https://doi.org/10.1073/pnas.2425390122](https://doi.org/10.1073/pnas.2425390122) (2025).","descriptionType":"TechnicalInfo"}],"geoLocations":[],"fundingReferences":[{"schemeUri":"https://ror.org","funderName":"Australian Research Council","funderIdentifier":"https://ror.org/05mmh0f86","funderIdentifierType":"ROR"},{"schemeUri":"https://ror.org","funderName":"National Health and Medical Research Council","funderIdentifier":"https://ror.org/011kf5r70","funderIdentifierType":"ROR"}],"xml":"<?xml version="1.0" encoding="UTF-8"?>
<resource xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns="http://datacite.org/schema/kernel-4" xsi:schemaLocation="http://datacite.org/schema/kernel-4 http://schema.datacite.org/meta/kernel-4/metadata.xsd">
  <identifier identifierType="DOI">10.5061/DRYAD.Z34TMPGQ9</identifier>
  <creators>
    <creator>
      <creatorName nameType="Personal">Yang, Ying</creatorName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0001-6009-4748</nameIdentifier>
      <affiliation affiliationIdentifier="https://ror.org/03r8z3t63" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">UNSW Sydney</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Ma, Yuanqing</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/03r8z3t63" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">UNSW Sydney</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Gooding, Justin</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/03r8z3t63" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">UNSW Sydney</affiliation>
    </creator>
  </creators>
  <titles>
    <title>
      Electrochemically controlled switching of dyes for enhanced
      super-resolution optical fluctuation imaging (EC-SOFI)
    </title>
  </titles>
  <publisher publisherIdentifier="https://ror.org/00x6h5n95" publisherIdentifierScheme="ROR" schemeURI="https://ror.org/">Dryad</publisher>
  <resourceType resourceTypeGeneral="Dataset">dataset</resourceType>
  <publicationYear>2025</publicationYear>
  <subjects>
    <subject subjectScheme="fos" schemeURI="https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf">FOS: Nano-technology</subject>
    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Optical microscopy</subject>
    <subject>super-resolution optical fluctuation imaging</subject>
    <subject>Electrochemical switching of dyes</subject>
  </subjects>
  <fundingReferences>
    <fundingReference>
      <funderName>Australian Research Council</funderName>
      <funderIdentifier funderIdentifierType="ROR">https://ror.org/05mmh0f86</funderIdentifier>
    </fundingReference>
    <fundingReference>
      <funderName>National Health and Medical Research Council</funderName>
      <funderIdentifier funderIdentifierType="ROR">https://ror.org/011kf5r70</funderIdentifier>
    </fundingReference>
  </fundingReferences>
  <contributors>
    <contributor contributorType="Sponsor">
      <contributorName>UNSW Sydney</contributorName>
    </contributor>
  </contributors>
  <dates>
    <date dateType="Created">2025-06-25T12:21:38Z</date>
    <date dateType="Submitted">2025-06-25T12:25:52Z</date>
    <date dateType="Issued">2025-07-18T00:00:00Z</date>
    <date dateType="Available">2025-07-18T00:00:00Z</date>
  </dates>
  <language>en</language>
  <relatedIdentifiers>
    <relatedIdentifier relationType="IsCitedBy" relatedIdentifierType="DOI">https://doi.org/10.1073/pnas.2425390122</relatedIdentifier>
    <relatedIdentifier relationType="IsCitedBy" relatedIdentifierType="DOI">https://doi.org/10.1101/2024.06.02.597055</relatedIdentifier>
  </relatedIdentifiers>
  <sizes>
    <size>5742285759 bytes</size>
  </sizes>
  <version>5</version>
  <rightsList>
    <rights rightsURI="https://spdx.org/licenses/CC0-1.0.html">Creative Commons Zero v1.0 Universal</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">
      In super-resolution optical fluctuation imaging (SOFI), molecules spaced
      closer than the diffraction limit can be identified through spatial and
      temporal correlation analysis of the fluorescence intensity fluctuations.
      The resolution and speed of SOFI imaging greatly depend on the
      characteristics of these fluorescence fluctuations. Fluorophores with
      stochastic and rapid fluorescence fluctuations are favourable for
      improving SOFI imaging resolution and speed, especially in high order
      cumulant analysis. Stochastic blinking dyes in specific buffers are
      well-suited for SOFI due to their high brightness and strong fluorescence
      fluctuations. However, their overall blinking rate can sometimes be too
      slow, presenting practical limitations for high-resolution and fast SOFI
      imaging. Furthermore, dye blinking can be non-uniform, creating
      nonlinearity in SOFI image. To address these challenges, we introduce
      electrochemically controlled dye switching for SOFI (EC-SOFI). By applying
      an oscillating electrochemical potential to a transparent electrode
      surface, we increase the overall dye switching rate and uniformity. Using
      Alexa 647 dye as an example, EC-SOFI reduces the average ON time by over
      3-fold and switching variance by more than 2-fold compared to conventional
      photochemical switching, achieving ~60 nm spatial resolution in 6th order
      EC-SOFI image. We further demonstrate that EC-SOFI achieves ~130 nm and
      ~80 nm resolution with 100 and 300 frames, respectively, enabling fast,
      large-area tile-scan super-resolution imaging. This advancement in EC-SOFI
      significantly enhances the practical potential of SOFI technique.
    </description>
    <description descriptionType="Methods">
      &lt;p&gt;&lt;strong&gt;Electrochemistry:&lt;/strong&gt;&lt;br&gt;Electrochemical measurements, including chronoamperometry and large-amplitude sinusoidal voltammetry, were performed using an SP-200 potentiostat (Bio-Logic, France) in a custom EC imaging chamber (Chamlide EC 22, Live Cell Instrument, Korea). The setup featured a three-electrode system: an ITO-coated coverslip (SPI Supplies, USA) as the working electrode, an Ag|AgCl|3M KCl reference electrode (LF-1-45, Innovative Instruments, USA), and a Pt mesh counter electrode. The chamber was mounted on the microscope stage for simultaneous EC control and fluorescence imaging.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Image acquisition:&lt;/strong&gt;&lt;br&gt;SOFI and STORM images were acquired on a Zeiss Elyra 7 Lattice SIM TIRF microscope with a 642 nm laser and sCMOS camera (edge 4.2, PCO). Illumination (EPI or TIRF) was controlled by shifting focus laterally in the objective's back focal plane. A 1.6× OptoVar lens reduced pixel size to 100 nm. Fluorescence was collected by the same 100× 1.46 NA oil objective and detected by the cooled sCMOS camera.&lt;/p&gt;
    </description>
    <description descriptionType="TechnicalInfo">
      # Data for: Electrochemically controlled switching of dyes for enhanced
      super-resolution optical fluctuation imaging (EC-SOFI) **Dataset DOI:**
      10.5061/dryad.z34tmpgq9\ **Corresponding Author:** To whom correspondence
      may be addressed. Email:
      [yuanqing.ma@unsw.edu.au](mailto:yuanqing.ma@unsw.edu.au) or
      [justin.gooding@unsw.edu.au](mailto:justin.gooding@unsw.edu.au). **1.
      Included Files** This dataset contains the raw imaging data used to
      generate Figures 1-4 in the associated publication. All image data are
      provided wither as .csv or`.tif` files inside `.zip` archives, directly
      exported from Zeiss Zen Blue.
      **Fig_1_single_molecule_photophysics_using_origami.zip**\ This folder
      includes the sinple molecule localization data in .csv format used for
      generating Figure 1, which shows single-molecule fluorescence trajectories
      using DNA origami labelled with Alexa 647. Four distinct regions of
      interest (ROI 1–4) are included for two experimental conditions: o   
      **Electrochemical (EC)**: 0.5 kW cm−2 642 nm laser were on, large
      amplitude square wave (LASV) potential was sinusoidally oscillated between
      −0.8 V to 0.1 V with a frequency of 10 Hz o    **UV Illumination**: 0.5 W
      cm−2 of UV laser and 0.5 kW cm−2 642 nm laser were on. These files of
      single-molecule localization results were directly exported from Zeiss Zen
      Blue, with each row corresponds to a detected fluorescent molecule. Below
      are the definitions of each column: * **Index**: Unique identifier for
      each localization event. * **First Frame**: Frame number when the molecule
      first appeared. * **Number Frames**: Total number of frames the molecule
      was detected. * **Frames Missing**: Number of skipped frames during a
      blinking event. * **X Position (nm)** / **Y Position (nm)**: Lateral
      coordinates of the molecule in nanometers. * **Precision (nm)**: Estimated
      localization uncertainty in X/Y, based on fitting. * **Photons**: Total
      photon count collected from the molecule. Reflects signal brightness. *
      **Background Variance**: Variability of background noise in the fitting
      region. * **Chi Square**: Goodness-of-fit indicator for the Gaussian
      model. * **PSF width (nm)**: Width of the fitted point spread function in
      nanometers. * **Channel**: Color or channel label used during imaging. *
      **Z-Slice**: Z-stack slice index (for 3D acquisitions). * **Z Position
      (nm)**: Axial (depth) position of the molecule in nanometers. * **Z
      Precision (nm)**: Estimated localization uncertainty in Z direction.
      **Fig_2_UV_vs_EC_SOFI.zip**\ Contains raw SOFI image sequences used to
      generate Figure 2, comparing UV- and EC-SOFI in fixed COS-7 cells. The
      data highlight differences in switching uniformity and kinetics.
      **Fig_3_High_order_SOFI.zip**\ Includes raw data used in high-order SOFI
      reconstruction shown in Figure 3. These sequences were used to calculate
      up to 6th-order SOFI images, demonstrating resolution enhancement using
      electrochemically modulated dye switching. **Fig_4_Tile_Scan.zip**\
      Contains one example for raw tiled image stacks used in Figure 4, more
      similar tiles were collected to achieve wide-area EC-SOFI imaging. ## 2.
      Recommended Software The .tif files provided in this dataset can be opened
      using freely available software: **Fiji (ImageJ distribution)** –
      Recommended for most users; supports stack viewing, analysis, and basic
      SOFI processing.\ Website:
      [https://imagej.net/software/fiji](https://imagej.net/software/fiji)
      **ImageJ** – A lightweight alternative to Fiji, with broad compatibility
      for viewing and simple operations.\ Website:
      [https://imagej.nih.gov/ij/](https://imagej.nih.gov/ij/) No proprietary
      software or plugins are needed to access the data. ## 3. Imaging settings:
      ·       **Excitation wavelength**: For most experiments, a 640 nm laser
      was used; UV conditions refer to 400 nm laser illumination. ·      
      **Applied potential**: In EC conditions, potentials were alternating
      between –0.8 V and +0.15 V versus Ag/AgCl/3M KCl reference. Additional
      experimental parameters such as sample preparation, buffer composition,
      and detailed EC and imaging settings are available in the
      manuscript's Methods section. ## 4. Citation If you use this dataset,
      please cite: Y. Yang,Y. Ma,R.D. Tilley,&amp; J.J. Gooding, 
      Electrochemically controlled switching of dyes for enhanced
      superresolution optical fluctuation imaging (EC-SOFI), Proc. Natl. Acad.
      Sci. U.S.A. 122 (28) e2425390122,
      [https://doi.org/10.1073/pnas.2425390122](https://doi.org/10.1073/pnas.2425390122) (2025).
    </description>
  </descriptions>
</resource>","url":"https://datadryad.org/dataset/doi:10.5061/dryad.z34tmpgq9","contentUrl":null,"metadataVersion":3,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":32,"viewsOverTime":[{"yearMonth":"2025-07","total":11},{"yearMonth":"2025-08","total":2},{"yearMonth":"2025-09","total":1},{"yearMonth":"2025-10","total":3},{"yearMonth":"2025-11","total":4},{"yearMonth":"2025-12","total":2},{"yearMonth":"2026-01","total":1},{"yearMonth":"2026-02","total":2},{"yearMonth":"2026-03","total":3},{"yearMonth":"2026-05","total":3}],"downloadCount":8,"downloadsOverTime":[{"yearMonth":"2025-07","total":1},{"yearMonth":"2025-08","total":2},{"yearMonth":"2025-09","total":0},{"yearMonth":"2025-10","total":4},{"yearMonth":"2025-11","total":0},{"yearMonth":"2025-12","total":0},{"yearMonth":"2026-01","total":0},{"yearMonth":"2026-02","total":0},{"yearMonth":"2026-03","total":1},{"yearMonth":"2026-05","total":0}],"referenceCount":0,"citationCount":2,"citationsOverTime":[{"year":"2026","total":2}],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2025-07-18T18:03:52.000Z","registered":"2025-07-18T18:03:53.000Z","published":"2025","updated":"2026-03-12T18:16:00.000Z"},"relationships":{"client":{"data":{"id":"dryad.dryad","type":"clients"}},"provider":{"data":{"id":"dryad","type":"providers"}},"media":{"data":{"id":"10.5061/dryad.z34tmpgq9","type":"media"}},"references":{"data":[]},"citations":{"data":[{"id":"10.1101/2024.06.02.597055","type":"dois"},{"id":"10.1073/pnas.2425390122","type":"dois"}]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}