{"data":{"id":"10.5061/dryad.x3ffbg7qx","type":"dois","attributes":{"doi":"10.5061/dryad.x3ffbg7qx","prefix":"10.5061","suffix":"dryad.x3ffbg7qx","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Yu, Yingpu","nameType":"Personal","givenName":"Yingpu","familyName":"Yu","affiliation":["Rockefeller University"],"nameIdentifiers":[]},{"name":"Kass, Maximilian A.","nameType":"Personal","givenName":"Maximilian A.","familyName":"Kass","affiliation":["Rockefeller University"],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0000-0001-8783-4574","nameIdentifierScheme":"ORCID"}]},{"name":"Zhang, Mengyin","nameType":"Personal","givenName":"Mengyin","familyName":"Zhang","affiliation":["Rockefeller University"],"nameIdentifiers":[]},{"name":"Youssef, Noor","nameType":"Personal","givenName":"Noor","familyName":"Youssef","affiliation":["Harvard Medical School"],"nameIdentifiers":[]},{"name":"Freije, Catherine A.","nameType":"Personal","givenName":"Catherine A.","familyName":"Freije","affiliation":["Rockefeller University"],"nameIdentifiers":[]},{"name":"Brock, Kelly P.","nameType":"Personal","givenName":"Kelly P.","familyName":"Brock","affiliation":["Harvard Medical School"],"nameIdentifiers":[]},{"name":"Aguado, Lauren C.","nameType":"Personal","givenName":"Lauren C.","familyName":"Aguado","affiliation":["Rockefeller University"],"nameIdentifiers":[]},{"name":"Seifert, Leon L.","nameType":"Personal","givenName":"Leon L.","familyName":"Seifert","affiliation":["Rockefeller University"],"nameIdentifiers":[]},{"name":"Venkittu, Sanjana","nameType":"Personal","givenName":"Sanjana","familyName":"Venkittu","affiliation":["Rockefeller University"],"nameIdentifiers":[]},{"name":"Hong, Xupeng","nameType":"Personal","givenName":"Xupeng","familyName":"Hong","affiliation":["Rockefeller University"],"nameIdentifiers":[]},{"name":"Shlomai, Amir","nameType":"Personal","givenName":"Amir","familyName":"Shlomai","affiliation":["Rockefeller University"],"nameIdentifiers":[]},{"name":"de Jong, Ype","nameType":"Personal","givenName":"Ype","familyName":"de Jong","affiliation":["Rockefeller University"],"nameIdentifiers":[]},{"name":"Marks, Debbie S.","nameType":"Personal","givenName":"Debbie S.","familyName":"Marks","affiliation":["Harvard Medical School"],"nameIdentifiers":[]},{"name":"Rice, Charles M.","nameType":"Personal","givenName":"Charles M.","familyName":"Rice","affiliation":["Rockefeller University"],"nameIdentifiers":[]},{"name":"Schneider, William M.","nameType":"Personal","givenName":"William M.","familyName":"Schneider","affiliation":["Rockefeller University"],"nameIdentifiers":[]}],"titles":[{"title":"Data from: Deep mutational scanning of HBV reveals a mechanism for cis preferential reverse transcription"}],"publisher":"Dryad","container":{},"publicationYear":2024,"subjects":[{"subject":"DMS"},{"subject":"HBV"},{"subject":"code"},{"subject":"FOS: Basic medicine","schemeUri":"https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf","subjectScheme":"fos"},{"subject":"FOS: Basic medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2023-07-17T19:03:50Z","dateType":"Created"},{"date":"2024-02-09T19:48:20Z","dateType":"Submitted"},{"date":"2024-02-29T00:00:00Z","dateType":"Issued"},{"date":"2024-02-29T00:00:00Z","dateType":"Available"}],"language":"en","types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsDerivedFrom","relatedIdentifier":"https://github.com/HBV-DMS/R1","relatedIdentifierType":"URL"},{"relationType":"IsCitedBy","relatedIdentifier":"10.1016/j.cell.2024.04.008","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["39828968 bytes"],"formats":[],"version":"8","rightsList":[{"rights":"Creative Commons Zero v1.0 Universal","rightsUri":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc0-1.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Hepatitis B virus (HBV) is a small double-stranded DNA virus that\n chronically infects 296 million people. Over half of its compact genome\n encodes protein in two overlapping reading frames, and during evolution,\n multiple selective pressures can act on shared nucleotides. This study\n combines an RNA-based HBV cell culture system with deep mutational\n scanning to uncouple cis- and trans-acting sequence requirements\n in the HBV genome. The results support a leaky ribosome scanning model for\n polymerase translation, provide a fitness map of the HBV polymerase at\n single nucleotide resolution, and identify conserved prolines adjacent to\n the HBV polymerase termination codon that stall ribosomes. Further\n experiments indicated that stalled ribosomes tether the nascent polymerase\n to its template RNA, ensuring cis-preferential RNA packaging and reverse\n transcription of the HBV genome.","descriptionType":"Abstract"},{"description":"# Data from: Deep mutational scanning of HBV reveals a mechanism for cis\n preferential reverse transcription [Access this dataset on\n Dryad](https://doi.org/10.5061/dryad.x3ffbg7qx) The purpose of this\n dataset is to provide the analysis software, the raw pre-processed\n experimental data files, and the processed result files used in the paper\n “Deep mutational scanning of HBV reveals a mechanism for cis preferential\n reverse transcription”. For detailed information on the experimental\n design, please refer to the paper. Briefly, mutants of the hepatitis B\n virus (HBV) were generated (input population) and transfected into cell\n cultures. In cell culture, HBV mutants were either depleted or enriched\n based on the effects of their mutations (output population). Afterwards,\n both input and output populations were sequenced to quantify the\n enrichment or depletion of each HBV mutant. From these sequencing results,\n so called codoncounts files were generated using barcoded-subamplicon\n sequencing software from Jesse Bloom’s dms_tools2 software package. These\n codoncounts files, simply put, state how often a certain mutant was\n present in the input or output population. The codoncounts files represent\n the main raw pre-processed files in this Dryad dataset\n (/data/codoncounts/). Mutants of HBV are split into groups corresponding\n to certain regions of the HBV genome that were mutated separately. For\n example, one group is called “TP” (in e.g. “TP_plasmid1_codoncounts.csv”),\n meaning that mutations were generated in the terminal protein domain (TP)\n of HBV in that experiment. The term “plasmid” in\n “TP_plasmid1_codoncounts.csv” denotes an input population data file, while\n “cell” in “TP_cell1_codoncounts.csv” indicates an output population data\n file. The number 1 refers to replicate one. There are usually three\n replicates, though the number can vary. A Jupyter Notebook, JNote.ipynb,\n is the primary analysis software file. This notebook takes the codoncounts\n files and calculates the enrichment score of each mutant. Using those\n results, JNote.ipynb generates the majority of figures in the paper,\n including the heatmaps in Figures 2B and 3A. JNote.ipynb also generates\n corresponding data tables with the plotted numeric values for each figure\n in CSV file format (/Fig/CSV/). Additionally, a second Jupyter Notebook is\n for a proline sliding window analysis related to Fig S5C in the paper.\n Please refer to the methods section of the paper for additional\n documentation. Furthermore, the file hbv_pol_model_parameter_details.txt\n contains configurations for natural sequence analysis related to figure\n S3B in the paper. ## Description of the data and file structure After\n unzipping, the dataset R1 contains: * **JNote.ipynb** - Main analysis\n software file. Takes codoncounts files (/data/codoncounts) as input to\n generate most figures and data tables (/Fig/CSV) in the paper. *\n /data/codoncounts - directory containing all codoncounts files. The first\n term denotes the region of the HBV genome that was mutated. The terms\n “cell” or “plasmid” denote the input or output populations, respectively.\n The number refers to the replicate numbering. For example,\n “Core_cell1_codoncounts.csv” is one of the three replicate mutant\n libraries of the Core region in the HBV genome after cell culture\n selection. Column “site” corresponds to the amino acid site position;\n column “wildtype” to the wildtype codon; columns \"AAA” to “TTT” to\n the mutation from wildtype, with the sequencing read count given as the\n row value. * /data/other - directory containing additional support files\n necessary for JNote.ipynb * /Fig/CSV – directory containing the\n corresponding data tables of the figures in the paper with the plotted\n numeric values in CSV format. The figure numbering follows the numbering\n in the paper. Please refer to the figure legends in the paper for\n additional information. * **Fig_2B.csv** – Deep mutational scanning of the\n Core region: heatmap X-axis -\u0026gt; column “site” –\u0026gt; amino acid site\n position Y-axis -\u0026gt; column “variable” -\u0026gt; variant mutation codon Color\n scale -\u0026gt; column \"logmfactor” -\u0026gt; log2 enrichment factor *\n **Fig_2C.csv** – Deep mutational scanning of the Core region: group\n analysis I X-axis -\u0026gt; column “group” –\u0026gt; certain variants grouped\n Y-axis -\u0026gt; column \" logmfactor” -\u0026gt; log2 enrichment factor *\n **Fig_2D.csv** – Deep mutational scanning of the Core region: group\n analysis II X-axis -\u0026gt; column “group” –\u0026gt; certain variants grouped\n Y-axis -\u0026gt; column \" logmfactor” -\u0026gt; log2 enrichment factor *\n **Fig_3A.csv** – Deep mutational scanning of Polymerase: heatmap X-axis\n -\u0026gt; column “site” –\u0026gt; amino acid site position Y-axis -\u0026gt; column\n “variable” -\u0026gt; variant mutation codon Color scale -\u0026gt; column\n \"logmfactor” -\u0026gt; log2 enrichment factor * **Fig_3D.csv** – Deep\n mutational scanning of Polymerase: group analysis X-axis -\u0026gt; column\n “group” –\u0026gt; certain variants grouped Y-axis -\u0026gt; column\n \"norm_abs_logmfactor” -\u0026gt; selective pressure * **Fig_S1B.csv** –\n Nucleotide mutation type bias analysis X-axis -\u0026gt; column “type” -\u0026gt;\n single nucleotide mutation type Y-axis -\u0026gt; column “frac” -\u0026gt;\n percentage of mutants * **Fig_S1C.csv** – Deep mutational scanning of the\n Core region: input plasmid counts heatmap X-axis -\u0026gt; column “site” –\u0026gt;\n amino acid site position Y-axis -\u0026gt; column “variable” -\u0026gt; variant\n mutation codon Color scale -\u0026gt; column \"pre_frac” * 1E6 -\u0026gt; log10\n plasmid counts per 1E6 reads * **Fig_S2B.csv** – Deep mutational scanning\n of the Core region: Kozak analysis I X-axis -\u0026gt; column “kozakscore”\n -\u0026gt; Kozak score of C1 Y-axis -\u0026gt; column \" logmfactor” -\u0026gt; log2\n enrichment factor of variants affecting C1 Kozak * **Fig_S2C.csv** – Deep\n mutational scanning of the Core region: Kozak analysis II X-axis -\u0026gt;\n column “kozakscore” -\u0026gt; Kozak score of J ORF start Y-axis -\u0026gt; column\n \" logmfactor” -\u0026gt; log2 enrichment factor of variants affecting J\n ORF start Kozak * **Fig_S2D.csv** –\u0026gt; Deep mutational scanning of the\n Core region: Kozak analysis III X-axis -\u0026gt; column “group” -\u0026gt; Group\n C/T or A/G Y-axis -\u0026gt; column \" logmfactor” -\u0026gt; log2 enrichment\n factor * **Fig_3A.csv** – Deep mutational scanning of Polymerase: input\n plasmid counts heatmap X-axis -\u0026gt; column “site” –\u0026gt; amino acid site\n position Y-axis -\u0026gt; column “variable” -\u0026gt; variant mutation codon Color\n scale -\u0026gt; column \"pre_frac” * 1E6 -\u0026gt; log10 plasmid counts per\n 1E6 reads * **Fig_3A.csv** – Fitness of polymerase variants obtained from\n natural sequences: heatmap X-axis -\u0026gt; column “pos” –\u0026gt; amino acid site\n position Y-axis -\u0026gt; column “mutant” -\u0026gt; variant mutation codon Color\n scale -\u0026gt; column \" prediction_independent” -\u0026gt; log2 factor\n natural enrichment * **PDB_files_Pol.zip** - contains files related to\n three-dimensional models of HBV polymerase (Pol) generated using\n AlphaFold2 related to Figure 3BC in the paper. *\n **hbv_pol_model_parameter_details.txt** - are parameters for the analysis\n of natural sequences in Figure S3B. * **proline_sliding_window.ipynb** -\n second Jupyter Notebook for a proline sliding window analysis for Figure\n S5C in the paper. ## Sharing/Access information Link to other publicly\n accessible locations of the data: *\n [https://github.com/HBV-DMS/R1](https://github.com/HBV-DMS/R1) ##\n Code/Software The main Jupyter Notebook, JNote.ipynb, can be run to\n re-analyze the experimental data starting from codoncounts files and to\n generate the majority of figures in the paper. The simplest way to run\n JNote.ipynb is to open JNote.ipynb through Google Colab by opening\n [https://colab.research.google.com/github/HBV-DMS/R1/blob/main/JNote.ipynb](https://colab.research.google.com/github/HBV-DMS/R1/blob/main/JNote.ipynb) and following the instructions in the notebook.","descriptionType":"TechnicalInfo"}],"geoLocations":[],"fundingReferences":[{"schemeUri":"https://ror.org","funderName":"National Institutes of Health","awardNumber":"R01AI091707","funderIdentifier":"https://ror.org/01cwqze88","funderIdentifierType":"ROR"},{"schemeUri":"https://ror.org","funderName":"National Institutes of Health","awardNumber":"R01AI143295","funderIdentifier":"https://ror.org/01cwqze88","funderIdentifierType":"ROR"},{"schemeUri":"https://ror.org","funderName":"National Institutes of Health","awardNumber":"R01AI150275","funderIdentifier":"https://ror.org/01cwqze88","funderIdentifierType":"ROR"},{"schemeUri":"https://ror.org","funderName":"German Center for Infection Research","funderIdentifier":"https://ror.org/028s4q594","funderIdentifierType":"ROR"},{"schemeUri":"https://ror.org","funderName":"Robertson Foundation","funderIdentifier":"https://ror.org/04h2x1077","funderIdentifierType":"ROR"}],"xml":"<?xml version="1.0" encoding="UTF-8"?>
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      <creatorName nameType="Personal">Yu, Yingpu</creatorName>
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      <creatorName nameType="Personal">Kass, Maximilian A.</creatorName>
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      <affiliation affiliationIdentifier="https://ror.org/0420db125" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Rockefeller University</affiliation>
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    <creator>
      <creatorName nameType="Personal">Zhang, Mengyin</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/0420db125" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Rockefeller University</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Youssef, Noor</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/03wevmz92" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Harvard Medical School</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Freije, Catherine A.</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/0420db125" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Rockefeller University</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Brock, Kelly P.</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/03wevmz92" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Harvard Medical School</affiliation>
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    <creator>
      <creatorName nameType="Personal">Aguado, Lauren C.</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/0420db125" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Rockefeller University</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Seifert, Leon L.</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/0420db125" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Rockefeller University</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Venkittu, Sanjana</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/0420db125" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Rockefeller University</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Hong, Xupeng</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/0420db125" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Rockefeller University</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Shlomai, Amir</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/0420db125" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Rockefeller University</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">de Jong, Ype</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/0420db125" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Rockefeller University</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Marks, Debbie S.</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/03wevmz92" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Harvard Medical School</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Rice, Charles M.</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/0420db125" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Rockefeller University</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Schneider, William M.</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/0420db125" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Rockefeller University</affiliation>
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  <titles>
    <title>
      Data from: Deep mutational scanning of HBV reveals a mechanism for cis
      preferential reverse transcription
    </title>
  </titles>
  <publisher publisherIdentifier="https://ror.org/00x6h5n95" publisherIdentifierScheme="ROR" schemeURI="https://ror.org/">Dryad</publisher>
  <resourceType resourceTypeGeneral="Dataset">dataset</resourceType>
  <publicationYear>2024</publicationYear>
  <subjects>
    <subject>DMS</subject>
    <subject>HBV</subject>
    <subject>code</subject>
    <subject subjectScheme="fos" schemeURI="https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf">FOS: Basic medicine</subject>
  </subjects>
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      <awardNumber>R01AI091707</awardNumber>
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      <funderName>German Center for Infection Research</funderName>
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      <funderName>Robertson Foundation</funderName>
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    </fundingReference>
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  <dates>
    <date dateType="Created">2023-07-17T19:03:50Z</date>
    <date dateType="Submitted">2024-02-09T19:48:20Z</date>
    <date dateType="Issued">2024-02-29T00:00:00Z</date>
    <date dateType="Available">2024-02-29T00:00:00Z</date>
  </dates>
  <language>en</language>
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  <descriptions>
    <description descriptionType="Abstract">
      Hepatitis B virus (HBV) is a small double-stranded DNA virus that
      chronically infects 296 million people. Over half of its compact genome
      encodes protein in two overlapping reading frames, and during evolution,
      multiple selective pressures can act on shared nucleotides. This study
      combines an RNA-based HBV cell culture system with deep mutational
      scanning to uncouple&amp;nbsp;cis- and trans-acting sequence requirements
      in the HBV genome. The results support a leaky ribosome scanning model for
      polymerase translation, provide a fitness map of the HBV polymerase at
      single nucleotide resolution, and identify conserved prolines adjacent to
      the HBV polymerase termination codon that stall ribosomes. Further
      experiments indicated that stalled ribosomes tether the nascent polymerase
      to its template RNA, ensuring cis-preferential RNA packaging and reverse
      transcription of the HBV genome.
    </description>
    <description descriptionType="TechnicalInfo">
      # Data from: Deep mutational scanning of HBV reveals a mechanism for cis
      preferential reverse transcription [Access this dataset on
      Dryad](https://doi.org/10.5061/dryad.x3ffbg7qx) The purpose of this
      dataset is to provide the analysis software, the raw pre-processed
      experimental data files, and the processed result files used in the paper
      “Deep mutational scanning of HBV reveals a mechanism for cis preferential
      reverse transcription”. For detailed information on the experimental
      design, please refer to the paper. Briefly, mutants of the hepatitis B
      virus (HBV) were generated (input population) and transfected into cell
      cultures. In cell culture, HBV mutants were either depleted or enriched
      based on the effects of their mutations (output population). Afterwards,
      both input and output populations were sequenced to quantify the
      enrichment or depletion of each HBV mutant. From these sequencing results,
      so called codoncounts files were generated using barcoded-subamplicon
      sequencing software from Jesse Bloom’s dms_tools2 software package. These
      codoncounts files, simply put, state how often a certain mutant was
      present in the input or output population. The codoncounts files represent
      the main raw pre-processed files in this Dryad dataset
      (/data/codoncounts/). Mutants of HBV are split into groups corresponding
      to certain regions of the HBV genome that were mutated separately. For
      example, one group is called “TP” (in e.g. “TP_plasmid1_codoncounts.csv”),
      meaning that mutations were generated in the terminal protein domain (TP)
      of HBV in that experiment. The term “plasmid” in
      “TP_plasmid1_codoncounts.csv” denotes an input population data file, while
      “cell” in “TP_cell1_codoncounts.csv” indicates an output population data
      file. The number 1 refers to replicate one. There are usually three
      replicates, though the number can vary. A Jupyter Notebook, JNote.ipynb,
      is the primary analysis software file. This notebook takes the codoncounts
      files and calculates the enrichment score of each mutant. Using those
      results, JNote.ipynb generates the majority of figures in the paper,
      including the heatmaps in Figures 2B and 3A. JNote.ipynb also generates
      corresponding data tables with the plotted numeric values for each figure
      in CSV file format (/Fig/CSV/). Additionally, a second Jupyter Notebook is
      for a proline sliding window analysis related to Fig S5C in the paper.
      Please refer to the methods section of the paper for additional
      documentation. Furthermore, the file hbv_pol_model_parameter_details.txt
      contains configurations for natural sequence analysis related to figure
      S3B in the paper. ## Description of the data and file structure After
      unzipping, the dataset R1 contains: * **JNote.ipynb** - Main analysis
      software file. Takes codoncounts files (/data/codoncounts) as input to
      generate most figures and data tables (/Fig/CSV) in the paper. *
      /data/codoncounts - directory containing all codoncounts files. The first
      term denotes the region of the HBV genome that was mutated. The terms
      “cell” or “plasmid” denote the input or output populations, respectively.
      The number refers to the replicate numbering. For example,
      “Core_cell1_codoncounts.csv” is one of the three replicate mutant
      libraries of the Core region in the HBV genome after cell culture
      selection. Column “site” corresponds to the amino acid site position;
      column “wildtype” to the wildtype codon; columns "AAA” to “TTT” to
      the mutation from wildtype, with the sequencing read count given as the
      row value. * /data/other - directory containing additional support files
      necessary for JNote.ipynb * /Fig/CSV – directory containing the
      corresponding data tables of the figures in the paper with the plotted
      numeric values in CSV format. The figure numbering follows the numbering
      in the paper. Please refer to the figure legends in the paper for
      additional information. * **Fig_2B.csv** – Deep mutational scanning of the
      Core region: heatmap X-axis -&gt; column “site” –&gt; amino acid site
      position Y-axis -&gt; column “variable” -&gt; variant mutation codon Color
      scale -&gt; column "logmfactor” -&gt; log2 enrichment factor *
      **Fig_2C.csv** – Deep mutational scanning of the Core region: group
      analysis I X-axis -&gt; column “group” –&gt; certain variants grouped
      Y-axis -&gt; column " logmfactor” -&gt; log2 enrichment factor *
      **Fig_2D.csv** – Deep mutational scanning of the Core region: group
      analysis II X-axis -&gt; column “group” –&gt; certain variants grouped
      Y-axis -&gt; column " logmfactor” -&gt; log2 enrichment factor *
      **Fig_3A.csv** – Deep mutational scanning of Polymerase: heatmap X-axis
      -&gt; column “site” –&gt; amino acid site position Y-axis -&gt; column
      “variable” -&gt; variant mutation codon Color scale -&gt; column
      "logmfactor” -&gt; log2 enrichment factor * **Fig_3D.csv** – Deep
      mutational scanning of Polymerase: group analysis X-axis -&gt; column
      “group” –&gt; certain variants grouped Y-axis -&gt; column
      "norm_abs_logmfactor” -&gt; selective pressure * **Fig_S1B.csv** –
      Nucleotide mutation type bias analysis X-axis -&gt; column “type” -&gt;
      single nucleotide mutation type Y-axis -&gt; column “frac” -&gt;
      percentage of mutants * **Fig_S1C.csv** – Deep mutational scanning of the
      Core region: input plasmid counts heatmap X-axis -&gt; column “site” –&gt;
      amino acid site position Y-axis -&gt; column “variable” -&gt; variant
      mutation codon Color scale -&gt; column "pre_frac” * 1E6 -&gt; log10
      plasmid counts per 1E6 reads * **Fig_S2B.csv** – Deep mutational scanning
      of the Core region: Kozak analysis I X-axis -&gt; column “kozakscore”
      -&gt; Kozak score of C1 Y-axis -&gt; column " logmfactor” -&gt; log2
      enrichment factor of variants affecting C1 Kozak * **Fig_S2C.csv** – Deep
      mutational scanning of the Core region: Kozak analysis II X-axis -&gt;
      column “kozakscore” -&gt; Kozak score of J ORF start Y-axis -&gt; column
      " logmfactor” -&gt; log2 enrichment factor of variants affecting J
      ORF start Kozak * **Fig_S2D.csv** –&gt; Deep mutational scanning of the
      Core region: Kozak analysis III X-axis -&gt; column “group” -&gt; Group
      C/T or A/G Y-axis -&gt; column " logmfactor” -&gt; log2 enrichment
      factor * **Fig_3A.csv** – Deep mutational scanning of Polymerase: input
      plasmid counts heatmap X-axis -&gt; column “site” –&gt; amino acid site
      position Y-axis -&gt; column “variable” -&gt; variant mutation codon Color
      scale -&gt; column "pre_frac” * 1E6 -&gt; log10 plasmid counts per
      1E6 reads * **Fig_3A.csv** – Fitness of polymerase variants obtained from
      natural sequences: heatmap X-axis -&gt; column “pos” –&gt; amino acid site
      position Y-axis -&gt; column “mutant” -&gt; variant mutation codon Color
      scale -&gt; column " prediction_independent” -&gt; log2 factor
      natural enrichment * **PDB_files_Pol.zip** - contains files related to
      three-dimensional models of HBV polymerase (Pol) generated using
      AlphaFold2 related to Figure 3BC in the paper. *
      **hbv_pol_model_parameter_details.txt** - are parameters for the analysis
      of natural sequences in Figure S3B. * **proline_sliding_window.ipynb** -
      second Jupyter Notebook for a proline sliding window analysis for Figure
      S5C in the paper. ## Sharing/Access information Link to other publicly
      accessible locations of the data: *
      [https://github.com/HBV-DMS/R1](https://github.com/HBV-DMS/R1) ##
      Code/Software The main Jupyter Notebook, JNote.ipynb, can be run to
      re-analyze the experimental data starting from codoncounts files and to
      generate the majority of figures in the paper. The simplest way to run
      JNote.ipynb is to open JNote.ipynb through Google Colab by opening
      [https://colab.research.google.com/github/HBV-DMS/R1/blob/main/JNote.ipynb](https://colab.research.google.com/github/HBV-DMS/R1/blob/main/JNote.ipynb) and following the instructions in the notebook.
    </description>
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