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Our previous work showed that surface\n expression of this protein complex could be induced by\n alloantibody-mediated complement activation through increased IL-1β\n synthesis, secretion, and autocrine/paracrine IL-1-mediated activation of\n NF-κB. In this article, we report that cultured human ECs express eight\n differently spliced IL-15 transcripts. Remarkably, IL-1β does not alter\n the expression level of any IL-15 transcript but induces surface\n expression independently of RNA polymerase II-mediated transcription while\n requiring new protein translation. Mechanistically, IL-1β causes an\n NF-κB-mediated reduction in the level of microRNA Let-7c-3p, thereby\n relieving a block of translation of IL-15 surface protein. Let7c-3p\n anti-miR can induce EC surface expression of IL-15/IL-15Rα in the absence\n of complement activation or of IL-1, enabling IL-15 transpresentation to\n boost CD8 T cell activation. Because of the complexity we have uncovered\n in IL-15 regulation, we recommend caution in interpreting increased total\n IL-15 mRNA or protein levels as a surrogate for\n transpresentation. ","descriptionType":"Abstract"},{"description":"# IL1b induction of endothelial surface IL15 by let7c3p downregulation\n Dataset DOI:\n [10.5061/dryad.sbcc2frgj](https://doi.org/10.5061/dryad.sbcc2frgj) ##\n Description of the data and file structure qRT-PCR performed per\n manuscript methods description under cytokine treatments as described. IF\n performed as described; captured on EVOS FL2 auto microscope. Quantified\n in ImageJ, reported in .xls file. Western Blotting was performed as\n described in the manuscript methods. ### Files and variables #### File:\n DICER_knockdown_WB_-_B-actin.png **Description:** DICER siRNA treatment WB\n beta-actin loading control #### File: DICER_knockdown_WB_-_DICER.png\n **Description:**  DICER siRNA WB probe for DICER\u0026gt; #### File:\n MFI_Data_values.xlsx **Description:**  MFI values for quantified\n immunofluorescence. Please reference the associated manuscript for\n treatment conditions. Values tabulated on per cell measured fluorescent\n intensity for each relevant fluorophore. Units: Fluorescent units\n Abbreviations: IL: Interleukin. DAPI: 4',6-diamidino-2-phenylindole,\n SELE: E-selectin. IFN: Interferon. DMSO: dimethyl sulfoxide. B11:\n Bay-117086. DRB: 5,6-dichloro-1-β-D-ribofuranosyl benzimidazole. CHX:\n Cycloheximide. si(TARGET): siRNA against Target. let7c Inh: Let-7c\n anti-miR #### File: Raw_qRT_PCR_Ct.xlsx **Description:**  Cycle times for\n qRT-PCR experiments. Units: Cycle Abbreviations: IL: Interleukin. GAPDH:\n Glyceraldehyde-3-phosphate dehydrogenase. DAPI:\n 4',6-diamidino-2-phenylindole, SELE: E-selectin. IFN: Interferon.\n DMSO: dimethyl sulfoxide. B11: Bay-117086. DRB:\n 5,6-dichloro-1-β-D-ribofuranosyl benzimidazole. CHX: Cycloheximide.\n si(TARGET): siRNA against Target. let7c Inh: Let-7c anti-miR. IP:\n Immunoprecipitation. #### File: p65_Knockdown_WB_-_B-actin.tif\n **Description:**  p65 knockdown beta actin loading control #### File:\n p65_Knockdown_WB_-_p65.tif **Description:** p65 knockdown p65 probe. ####\n File: FACS_Experiments.zip **Description:** pSTAT5A flow cytometry\n experiments containing FCS files. APC: CD8. PE: Phospho-STAT5A. #### File:\n IF_Images.zip **Description:** RAW images of immunofluorescence\n experiments. ## Code/software ImageJ, flow cytometry analysis software. No\n specialty software required.","descriptionType":"TechnicalInfo"}],"geoLocations":[],"fundingReferences":[{"schemeUri":"https://ror.org","funderName":"National Institute of Allergy and Infectious Diseases","awardNumber":"5R21AI180187","funderIdentifier":"https://ror.org/043z4tv69","funderIdentifierType":"ROR"}],"xml":"<?xml version="1.0" encoding="UTF-8"?>
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      <creatorName nameType="Personal">Pober, Jordan</creatorName>
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      <affiliation affiliationIdentifier="https://ror.org/03v76x132" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Yale University</affiliation>
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  <titles>
    <title>IL1b induction of endothelial surface IL15 by let7c3p downregulation</title>
  </titles>
  <publisher publisherIdentifier="https://ror.org/00x6h5n95" publisherIdentifierScheme="ROR" schemeURI="https://ror.org/">Dryad</publisher>
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    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Immunofluorescence</subject>
    <subject>real time PCR</subject>
    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Western blot</subject>
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      <funderName>National Institute of Allergy and Infectious Diseases</funderName>
      <funderIdentifier funderIdentifierType="ROR">https://ror.org/043z4tv69</funderIdentifier>
      <awardNumber>5R21AI180187</awardNumber>
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    <date dateType="Created">2024-10-28T21:37:31Z</date>
    <date dateType="Submitted">2024-10-28T21:38:16Z</date>
    <date dateType="Issued">2025-10-21T00:00:00Z</date>
    <date dateType="Available">2025-10-21T00:00:00Z</date>
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  <descriptions>
    <description descriptionType="Abstract">
      Expression of IL-15 on the surface of human graft endothelial cells (ECs)
      bound to the IL-15Rα subunit can increase the activation of CTLs,
      potentiating allograft rejection. Our previous work showed that surface
      expression of this protein complex could be induced by
      alloantibody-mediated complement activation through increased IL-1β
      synthesis, secretion, and autocrine/paracrine IL-1-mediated activation of
      NF-κB. In this article, we report that cultured human ECs express eight
      differently spliced IL-15 transcripts. Remarkably, IL-1β does not alter
      the expression level of any IL-15 transcript but induces surface
      expression independently of RNA polymerase II-mediated transcription while
      requiring new protein translation. Mechanistically, IL-1β causes an
      NF-κB-mediated reduction in the level of microRNA Let-7c-3p, thereby
      relieving a block of translation of IL-15 surface protein. Let7c-3p
      anti-miR can induce EC surface expression of IL-15/IL-15Rα in the absence
      of complement activation or of IL-1, enabling IL-15 transpresentation to
      boost CD8 T cell activation. Because of the complexity we have uncovered
      in IL-15 regulation, we recommend caution in interpreting increased total
      IL-15 mRNA or protein levels as a surrogate for
      transpresentation.&amp;nbsp;
    </description>
    <description descriptionType="TechnicalInfo">
      # IL1b induction of endothelial surface IL15 by let7c3p downregulation
      Dataset DOI:
      [10.5061/dryad.sbcc2frgj](https://doi.org/10.5061/dryad.sbcc2frgj) ##
      Description of the data and file structure qRT-PCR performed per
      manuscript methods description under cytokine treatments as described. IF
      performed as described; captured on EVOS FL2 auto microscope. Quantified
      in ImageJ, reported in .xls file. Western Blotting was performed as
      described in the manuscript methods. ### Files and variables #### File:
      DICER_knockdown_WB_-_B-actin.png **Description:** DICER siRNA treatment WB
      beta-actin loading control #### File: DICER_knockdown_WB_-_DICER.png
      **Description:**  DICER siRNA WB probe for DICER&gt; #### File:
      MFI_Data_values.xlsx **Description:**  MFI values for quantified
      immunofluorescence. Please reference the associated manuscript for
      treatment conditions. Values tabulated on per cell measured fluorescent
      intensity for each relevant fluorophore. Units: Fluorescent units
      Abbreviations: IL: Interleukin. DAPI: 4',6-diamidino-2-phenylindole,
      SELE: E-selectin. IFN: Interferon. DMSO: dimethyl sulfoxide. B11:
      Bay-117086. DRB: 5,6-dichloro-1-β-D-ribofuranosyl benzimidazole. CHX:
      Cycloheximide. si(TARGET): siRNA against Target. let7c Inh: Let-7c
      anti-miR #### File: Raw_qRT_PCR_Ct.xlsx **Description:**  Cycle times for
      qRT-PCR experiments. Units: Cycle Abbreviations: IL: Interleukin. GAPDH:
      Glyceraldehyde-3-phosphate dehydrogenase. DAPI:
      4',6-diamidino-2-phenylindole, SELE: E-selectin. IFN: Interferon.
      DMSO: dimethyl sulfoxide. B11: Bay-117086. DRB:
      5,6-dichloro-1-β-D-ribofuranosyl benzimidazole. CHX: Cycloheximide.
      si(TARGET): siRNA against Target. let7c Inh: Let-7c anti-miR. IP:
      Immunoprecipitation. #### File: p65_Knockdown_WB_-_B-actin.tif
      **Description:**  p65 knockdown beta actin loading control #### File:
      p65_Knockdown_WB_-_p65.tif **Description:** p65 knockdown p65 probe. ####
      File: FACS_Experiments.zip **Description:** pSTAT5A flow cytometry
      experiments containing FCS files. APC: CD8. PE: Phospho-STAT5A. #### File:
      IF_Images.zip **Description:** RAW images of immunofluorescence
      experiments. ## Code/software ImageJ, flow cytometry analysis software. No
      specialty software required.
    </description>
  </descriptions>
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