{"data":{"id":"10.5061/dryad.r7sqv9sq4","type":"dois","attributes":{"doi":"10.5061/dryad.r7sqv9sq4","prefix":"10.5061","suffix":"dryad.r7sqv9sq4","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Tian, Zhengming","nameType":"Personal","givenName":"Zhengming","familyName":"Tian","affiliation":["Capital Medical University"],"nameIdentifiers":[]},{"name":"Li, Yuning","nameType":"Personal","givenName":"Yuning","familyName":"Li","affiliation":["Capital Medical University"],"nameIdentifiers":[]},{"name":"Jin, Feiyang","nameType":"Personal","givenName":"Feiyang","familyName":"Jin","affiliation":["Capital Medical University"],"nameIdentifiers":[]},{"name":"Xu, Zirui","nameType":"Personal","givenName":"Zirui","familyName":"Xu","affiliation":["Capital Medical University"],"nameIdentifiers":[]},{"name":"Gu, Yakun","nameType":"Personal","givenName":"Yakun","familyName":"Gu","affiliation":["Capital Medical University"],"nameIdentifiers":[]},{"name":"Guo, Mengyuan","nameType":"Personal","givenName":"Mengyuan","familyName":"Guo","affiliation":["Capital Medical University"],"nameIdentifiers":[]},{"name":"Shao, Qianqian","nameType":"Personal","givenName":"Qianqian","familyName":"Shao","affiliation":["Capital Medical University"],"nameIdentifiers":[]},{"name":"Liu, Yingxia","nameType":"Personal","givenName":"Yingxia","familyName":"Liu","affiliation":["Capital Medical University"],"nameIdentifiers":[]},{"name":"Luo, Hanjiang","nameType":"Personal","givenName":"Hanjiang","familyName":"Luo","affiliation":["Guilin Medical University"],"nameIdentifiers":[]},{"name":"Wang, Yue","nameType":"Personal","givenName":"Yue","familyName":"Wang","affiliation":["BGI Research"],"nameIdentifiers":[]},{"name":"Zhang, Suyu","nameType":"Personal","givenName":"Suyu","familyName":"Zhang","affiliation":["BGI Research"],"nameIdentifiers":[]},{"name":"Yang, Chenlu","nameType":"Personal","givenName":"Chenlu","familyName":"Yang","affiliation":["BGI Research"],"nameIdentifiers":[]},{"name":"Liu, Xin","nameType":"Personal","givenName":"Xin","familyName":"Liu","affiliation":["BGI Research"],"nameIdentifiers":[]},{"name":"Ji, Xunming","nameType":"Personal","givenName":"Xunming","familyName":"Ji","affiliation":["Capital Medical University"],"nameIdentifiers":[]},{"name":"Liu, Jia","nameType":"Personal","givenName":"Jia","familyName":"Liu","affiliation":["Capital Medical University"],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0000-0001-6711-3841","nameIdentifierScheme":"ORCID"}]}],"titles":[{"title":"Brain-derived exosomal hemoglobin transfer contributes to neuronal mitochondrial homeostasis under hypoxia"}],"publisher":"Dryad","container":{},"publicationYear":2025,"subjects":[{"subject":"FOS: Basic medicine","schemeUri":"https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf","subjectScheme":"fos"},{"subject":"FOS: Basic medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Hypoxia","schemeUri":"https://github.com/PLOS/plos-thesaurus","subjectScheme":"PLOS Subject Area Thesaurus"},{"subject":"Neuroscience","schemeUri":"https://github.com/PLOS/plos-thesaurus","subjectScheme":"PLOS Subject Area Thesaurus"},{"subject":"Mitochondria","schemeUri":"https://github.com/PLOS/plos-thesaurus","subjectScheme":"PLOS Subject Area Thesaurus"}],"contributors":[],"dates":[{"date":"2025-06-05T16:16:05Z","dateType":"Created"},{"date":"2025-06-05T16:17:49Z","dateType":"Submitted"},{"date":"2025-06-18T00:00:00Z","dateType":"Issued"},{"date":"2025-06-18T00:00:00Z","dateType":"Available"}],"language":"en","types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsCitedBy","relatedIdentifier":"10.7554/elife.99986.2","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["1967611565 bytes"],"formats":[],"version":"3","rightsList":[{"rights":"Creative Commons Zero v1.0 Universal","rightsUri":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc0-1.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Hypoxia is an important physiological stress that causes nerve injuries\n and several brain diseases. However, the mechanism of brain response to\n hypoxia remains unclear, thus limiting the development of interventional\n strategies. This study conducted combined analyses of single-nucleus\n transcriptome sequencing and extracellular vesicle transcriptome\n sequencing on hypoxic mouse brains, described cell-cell communication in\n the brain under hypoxia from intercellular and extracellular dimensions,\n confirmed that hemoglobin mRNA was transferred from non-neuronal cells to\n neurons, and eventually expressed. Then we further explored the role of\n exosomal hemoglobin transfer in vitro, using human-derived cell lines, and\n clarified that hypoxia promoted the transfer and expression of exosomal\n hemoglobin between endothelial cells and neurons. And we found the vital\n function of exosomal hemoglobin to protect against neurological injury by\n maintaining mitochondrial homeostasis in neurons. In conclusion, this\n study identified a novel mechanism of ‘mutual aid’ in hypoxia responses in\n the brain, involving exosomal hemoglobin transfer, clarified the important\n role of exosomal communication in the process of brain stress response,\n and provided a novel interventional perspective for hypoxia-related brain\n diseases.","descriptionType":"Abstract"},{"description":"# Brain-derived exosomal hemoglobin transfer contributes to neuronal\n mitochondrial homeostasis under hypoxia Dataset DOI:\n [10.5061/dryad.r7sqv9sq4](10.5061/dryad.r7sqv9sq4) ## Description of the\n data and file structure This dataset contains processed single-nucleus RNA\n sequencing (snRNA-seq) data obtained from mouse brain tissue under chronic\n hypoxia. Two experimental groups were included: a control group and a\n group exposed to hypoxia for 14 days. The data were generated to\n investigate transcriptional alterations and cell-type-level responses\n associated with prolonged hypoxic stress in the brain. ### Files and\n variables #### File: snRNA\\_Con\\_H14d\\_seurat.rds **Description:**  *\n snRNA_Con_H14d_seurat.rds: An R object in Seurat format containing the\n processed single-nucleus RNA-seq data for two mouse brain experimental\n groups: * \"Con\": control group * \"H14d\": mice exposed\n to hypoxia for 28 days #### **Variables contained within the .rds Seurat\n object include:** * **Expression matrix (@assays$RNA@data)** * Normalized\n log-transformed gene expression (log-normalized counts) * Rows: genes;\n Columns: single nuclei * Unit: log-normalized counts * Missing values: not\n applicable (genes with zero counts not explicitly removed) * **Metadata\n (@meta.data)** * orig.ident: Sample group identity (Con or H14d) *\n nFeature_RNA: Number of genes detected per nucleus * nCount_RNA: Total RNA\n counts per nucleus * seurat_clusters: Cluster label assigned by Seurat\n clustering * Other standard Seurat metadata fields ## Code/software This\n dataset is provided as an .rds file containing a Seurat object. To load\n and explore the data, the following free and open-source software is\n required: * **R** (version ≥ 4.1.0) * **Seurat** package (version ≥ 4.3.0)\n Additional packages commonly used in the downstream analysis include: *\n **dplyr**, **ggplot2**, **patchwork** – for data manipulation and\n visualization * **CellChat** (version ≥ 1.6.1) – for intercellular\n communication analysis * **SingleR** (optional) – for reference-based cell\n type annotation ## Access information NA","descriptionType":"TechnicalInfo"}],"geoLocations":[],"fundingReferences":[{"schemeUri":"https://ror.org","funderName":"National Natural Science Foundation of China","awardNumber":"32100925","funderIdentifier":"https://ror.org/01h0zpd94","funderIdentifierType":"ROR"}],"xml":"<?xml version="1.0" encoding="UTF-8"?>
<resource xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns="http://datacite.org/schema/kernel-4" xsi:schemaLocation="http://datacite.org/schema/kernel-4 http://schema.datacite.org/meta/kernel-4/metadata.xsd">
  <identifier identifierType="DOI">10.5061/DRYAD.R7SQV9SQ4</identifier>
  <creators>
    <creator>
      <creatorName nameType="Personal">Tian, Zhengming</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/013xs5b60" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Capital Medical University</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Li, Yuning</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/013xs5b60" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Capital Medical University</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Jin, Feiyang</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/013xs5b60" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Capital Medical University</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Xu, Zirui</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/013xs5b60" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Capital Medical University</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Gu, Yakun</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/013xs5b60" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Capital Medical University</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Guo, Mengyuan</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/013xs5b60" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Capital Medical University</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Shao, Qianqian</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/013xs5b60" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Capital Medical University</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Liu, Yingxia</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/013xs5b60" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Capital Medical University</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Luo, Hanjiang</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/000prga03" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Guilin Medical University</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Wang, Yue</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/05gsxrt27" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">BGI Research</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Zhang, Suyu</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/05gsxrt27" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">BGI Research</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Yang, Chenlu</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/05gsxrt27" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">BGI Research</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Liu, Xin</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/05gsxrt27" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">BGI Research</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Ji, Xunming</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/013xs5b60" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Capital Medical University</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Liu, Jia</creatorName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0001-6711-3841</nameIdentifier>
      <affiliation affiliationIdentifier="https://ror.org/013xs5b60" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Capital Medical University</affiliation>
    </creator>
  </creators>
  <titles>
    <title>
      Brain-derived exosomal hemoglobin transfer contributes to neuronal
      mitochondrial homeostasis under hypoxia
    </title>
  </titles>
  <publisher publisherIdentifier="https://ror.org/00x6h5n95" publisherIdentifierScheme="ROR" schemeURI="https://ror.org/">Dryad</publisher>
  <resourceType resourceTypeGeneral="Dataset">dataset</resourceType>
  <publicationYear>2025</publicationYear>
  <subjects>
    <subject subjectScheme="fos" schemeURI="https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf">FOS: Basic medicine</subject>
    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Hypoxia</subject>
    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Neuroscience</subject>
    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Mitochondria</subject>
  </subjects>
  <fundingReferences>
    <fundingReference>
      <funderName>National Natural Science Foundation of China</funderName>
      <funderIdentifier funderIdentifierType="ROR">https://ror.org/01h0zpd94</funderIdentifier>
      <awardNumber>32100925</awardNumber>
    </fundingReference>
  </fundingReferences>
  <dates>
    <date dateType="Created">2025-06-05T16:16:05Z</date>
    <date dateType="Submitted">2025-06-05T16:17:49Z</date>
    <date dateType="Issued">2025-06-18T00:00:00Z</date>
    <date dateType="Available">2025-06-18T00:00:00Z</date>
  </dates>
  <language>en</language>
  <relatedIdentifiers>
    <relatedIdentifier relationType="IsCitedBy" relatedIdentifierType="DOI">https://doi.org/10.7554/eLife.99986.2</relatedIdentifier>
  </relatedIdentifiers>
  <sizes>
    <size>1967611565 bytes</size>
  </sizes>
  <version>3</version>
  <rightsList>
    <rights rightsURI="https://spdx.org/licenses/CC0-1.0.html">Creative Commons Zero v1.0 Universal</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">
      Hypoxia is an important physiological stress that causes nerve injuries
      and several brain diseases. However, the mechanism of brain response to
      hypoxia remains unclear, thus limiting the development of interventional
      strategies. This study conducted combined analyses of single-nucleus
      transcriptome sequencing and extracellular vesicle transcriptome
      sequencing on hypoxic mouse brains, described cell-cell communication in
      the brain under hypoxia from intercellular and extracellular dimensions,
      confirmed that hemoglobin mRNA was transferred from non-neuronal cells to
      neurons, and eventually expressed. Then we further explored the role of
      exosomal hemoglobin transfer in vitro, using human-derived cell lines, and
      clarified that hypoxia promoted the transfer and expression of exosomal
      hemoglobin between endothelial cells and neurons. And we found the vital
      function of exosomal hemoglobin to protect against neurological injury by
      maintaining mitochondrial homeostasis in neurons. In conclusion, this
      study identified a novel mechanism of ‘mutual aid’ in hypoxia responses in
      the brain, involving exosomal hemoglobin transfer, clarified the important
      role of exosomal communication in the process of brain stress response,
      and provided a novel interventional perspective for hypoxia-related brain
      diseases.
    </description>
    <description descriptionType="TechnicalInfo">
      # Brain-derived exosomal hemoglobin transfer contributes to neuronal
      mitochondrial homeostasis under hypoxia Dataset DOI:
      [10.5061/dryad.r7sqv9sq4](10.5061/dryad.r7sqv9sq4) ## Description of the
      data and file structure This dataset contains processed single-nucleus RNA
      sequencing (snRNA-seq) data obtained from mouse brain tissue under chronic
      hypoxia. Two experimental groups were included: a control group and a
      group exposed to hypoxia for 14 days. The data were generated to
      investigate transcriptional alterations and cell-type-level responses
      associated with prolonged hypoxic stress in the brain. ### Files and
      variables #### File: snRNA\_Con\_H14d\_seurat.rds **Description:**  *
      snRNA_Con_H14d_seurat.rds: An R object in Seurat format containing the
      processed single-nucleus RNA-seq data for two mouse brain experimental
      groups: * "Con": control group * "H14d": mice exposed
      to hypoxia for 28 days #### **Variables contained within the .rds Seurat
      object include:** * **Expression matrix (@assays$RNA@data)** * Normalized
      log-transformed gene expression (log-normalized counts) * Rows: genes;
      Columns: single nuclei * Unit: log-normalized counts * Missing values: not
      applicable (genes with zero counts not explicitly removed) * **Metadata
      (@meta.data)** * orig.ident: Sample group identity (Con or H14d) *
      nFeature_RNA: Number of genes detected per nucleus * nCount_RNA: Total RNA
      counts per nucleus * seurat_clusters: Cluster label assigned by Seurat
      clustering * Other standard Seurat metadata fields ## Code/software This
      dataset is provided as an .rds file containing a Seurat object. To load
      and explore the data, the following free and open-source software is
      required: * **R** (version ≥ 4.1.0) * **Seurat** package (version ≥ 4.3.0)
      Additional packages commonly used in the downstream analysis include: *
      **dplyr**, **ggplot2**, **patchwork** – for data manipulation and
      visualization * **CellChat** (version ≥ 1.6.1) – for intercellular
      communication analysis * **SingleR** (optional) – for reference-based cell
      type annotation ## Access information NA
    </description>
  </descriptions>
</resource>","url":"https://datadryad.org/dataset/doi:10.5061/dryad.r7sqv9sq4","contentUrl":null,"metadataVersion":2,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":32,"viewsOverTime":[{"yearMonth":"2025-06","total":8},{"yearMonth":"2025-07","total":6},{"yearMonth":"2025-09","total":2},{"yearMonth":"2025-10","total":2},{"yearMonth":"2025-11","total":2},{"yearMonth":"2025-12","total":2},{"yearMonth":"2026-01","total":2},{"yearMonth":"2026-05","total":3},{"yearMonth":"2026-09","total":5}],"downloadCount":4,"downloadsOverTime":[{"yearMonth":"2025-06","total":2},{"yearMonth":"2025-07","total":1},{"yearMonth":"2025-09","total":0},{"yearMonth":"2025-10","total":0},{"yearMonth":"2025-11","total":0},{"yearMonth":"2025-12","total":0},{"yearMonth":"2026-01","total":1},{"yearMonth":"2026-05","total":0},{"yearMonth":"2026-09","total":0}],"referenceCount":0,"citationCount":3,"citationsOverTime":[{"year":"2025","total":1},{"year":"2026","total":2}],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2025-06-18T19:31:58.000Z","registered":"2025-06-18T19:31:58.000Z","published":"2025","updated":"2026-01-29T00:02:56.000Z"},"relationships":{"client":{"data":{"id":"dryad.dryad","type":"clients"}},"provider":{"data":{"id":"dryad","type":"providers"}},"media":{"data":{"id":"10.5061/dryad.r7sqv9sq4","type":"media"}},"references":{"data":[]},"citations":{"data":[{"id":"10.7554/elife.99986.3","type":"dois"},{"id":"10.7554/elife.99986","type":"dois"},{"id":"10.7554/elife.99986.2","type":"dois"}]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}