{"data":{"id":"10.5061/dryad.pzgmsbcxn","type":"dois","attributes":{"doi":"10.5061/dryad.pzgmsbcxn","prefix":"10.5061","suffix":"dryad.pzgmsbcxn","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Chen, Ying","nameType":"Personal","givenName":"Ying","familyName":"Chen","affiliation":["First Hospital of China Medical University"],"nameIdentifiers":[]},{"name":"Li, Yanqiu","nameType":"Personal","givenName":"Yanqiu","familyName":"Li","affiliation":["First Hospital of China Medical University"],"nameIdentifiers":[]},{"name":"Zhang, Deyu","nameType":"Personal","givenName":"Deyu","familyName":"Zhang","affiliation":["Shengjing Hospital of China Medical University"],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0000-0003-0952-1980","nameIdentifierScheme":"ORCID"}]}],"titles":[{"title":"Data from: TNFAIP9 protects against the development of the early stage of chronic kidney disease: Focus on inflammation and fibrosis"}],"publisher":"Dryad","container":{},"publicationYear":2024,"subjects":[{"subject":"FOS: Basic medicine","schemeUri":"https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf","subjectScheme":"fos"},{"subject":"FOS: Basic medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Chronic kidney disease","schemeUri":"https://github.com/PLOS/plos-thesaurus","subjectScheme":"PLOS Subject Area Thesaurus"},{"subject":"Gene therapy","schemeUri":"https://github.com/PLOS/plos-thesaurus","subjectScheme":"PLOS Subject Area Thesaurus"},{"subject":"Inflammation","schemeUri":"https://github.com/PLOS/plos-thesaurus","subjectScheme":"PLOS Subject Area Thesaurus"},{"subject":"Fibrosis","schemeUri":"https://github.com/PLOS/plos-thesaurus","subjectScheme":"PLOS Subject Area Thesaurus"}],"contributors":[],"dates":[{"date":"2024-11-25T07:40:13Z","dateType":"Created"},{"date":"2024-11-25T07:40:27Z","dateType":"Submitted"},{"date":"2024-12-31T00:00:00Z","dateType":"Issued"},{"date":"2024-12-31T00:00:00Z","dateType":"Available"}],"language":"en","types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsCitedBy","relatedIdentifier":"10.1371/journal.pone.0325334","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["197210 bytes"],"formats":[],"version":"6","rightsList":[{"rights":"Creative Commons Zero v1.0 Universal","rightsUri":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc0-1.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Tumor necrosis factor alpha-induced protein 9 (TNFAIP9) is a crucial\n effector molecule that protects cells from inflammatory and metabolic\n damage. This study focuses on investigating the role and regulatory\n mechanisms of TNFAIP9 in the progression of chronic kidney disease (CKD).\n By analyzing CKD-related datasets from the GEO database, we discovered\n that TNFAIP9 was upregulated in CKD patients and CKD mice compared to\n their normal controls. To elucidate the functional role of TNFAIP9, we\n established a mouse model of CKD through a two-step 5/6 nephrectomy (Nx).\n The experimental mice were transduced with an adenoviral vector to express\n TNFAIP9. The results showed that mice undergoing 5/6-Nx developed evident\n renal impairment, inflammation, and fibrosis. Overexpression of TNFAIP9\n resulted in the remission of renal impairment, a decreased inflammatory\n response, and a reduced expression of fibrotic markers. In vitro, human\n renal tubular epithelial human kidney-2 (HK-2) cells were exposed to tumor\n necrosis factor-alpha (TNF-α) or transforming growth factor-beta (TGF-β)\n to simulate inflammatory and fibrotic conditions, respectively. Then, the\n overexpression plasmid or small interfering RNA (siRNA) targeting TNFAIP9\n was transfected into HK-2 cells to either overexpress or knock down the\n target protein. Overexpression of TNFAIP9 reduced the TNF-α-induced\n inflammatory response, while its knockdown amplified it. Likewise,\n overexpression of TNFAIP9 decreased the TGF-β-induced fibrosis, whereas\n its knockdown heightened it. In summary, it is suggested that TNFAIP9\n plays a protective role against the early stage of CKD by suppressing\n renal inflammation and fibrosis. Therefore, targeting TNFAIP9 could be a\n promising therapeutic approach for CKD.","descriptionType":"Abstract"},{"description":"The current dataset provides raw data that can be used to form\n the histogram, including quantitative results of\n immunofluorescence/immunohistochemistry/pathology scores, kit results, and\n normalized quantitative results of PCR and Western blot.","descriptionType":"Methods"},{"description":"# Data from: TNFAIP9 protects against the development of the early stage\n of chronic kidney disease: focus on inflammation and fibrosis\n **Introduction** This dataset includes raw data that can be used to form\n the histogram, including quantitative results of\n immunofluorescence/immunohistochemistry/pathology scores, kit results, and\n normalized quantitative results of PCR and Western blot to support the\n study “TNFAIP9 protects against the development of the early stage of\n chronic kidney disease: focus on inflammation and fibrosis”, which is\n currently in press. **File list** Original Data, in pptx format. **Author\n Information** Name: Ying Chen Institution: First Hospital of China Medical\n University, Shenyang, China Co-investigator 1 Name: Yanqiu Li Institution:\n First Hospital of China Medical University, Shenyang, China Corresponding\n author Name: Dr. Deyu Zhang Institution: Shengjing Hospital of China\n Medical University, Shenyang, China **Description of the data and file\n structure** The data presented in this dataset underpins the quantitative\n statistics for each graph featured in the publication, thereby ensuring\n that the dataset is derived from the figures reported in the publication.\n The included data encompasses the following quantitative results: Kit\n tests yielded serum levels of creatinine and urea nitrogen; Masson’s\n trichrome staining quantified the percentage of fibrotic areas relative to\n the total area. ELISA assays determined concentrations of TNF-α, IL-6, and\n MCP-1 within kidney tissue or cell supernatants. Western blot analysis\n provided relative quantification values based on grayscale measurements\n for α-SMA, Fibronectin, TNFAIP9, iNOS, COX-2, p-p65ser536, and Collagen\n III. The percentage of positively stained areas for TNFAIP9, Collagen III,\n and NGAL was assessed through immunohistochemistry. Real-time PCR\n facilitated measurement of relative mRNA content for TNFAIP9. Biochemical\n assays recorded arterial pressure as well as 24-hour urinary protein\n excretion from the animals involved in this study. Hematoxylin-eosin (HE)\n staining offered a quantitative score reflecting tissue injury severity.\n Additionally, immunofluorescence techniques were employed to quantify the\n percentage of positively stained cells. **Acknowledgments** This study was\n funded by the Postdoctoral Science Foundation of China (2021M703608). The\n funders had no role in study design, data collection and analysis,\n decision to publish, or preparation of the manuscript.","descriptionType":"TechnicalInfo"}],"geoLocations":[],"fundingReferences":[{"schemeUri":"https://ror.org","funderName":"National Natural Science Foundation of China","awardNumber":"2021M703608","funderIdentifier":"https://ror.org/01h0zpd94","funderIdentifierType":"ROR"}],"xml":"<?xml version="1.0" encoding="UTF-8"?>
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      <creatorName nameType="Personal">Chen, Ying</creatorName>
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    </creator>
    <creator>
      <creatorName nameType="Personal">Li, Yanqiu</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/04wjghj95" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">First Hospital of China Medical University</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Zhang, Deyu</creatorName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0003-0952-1980</nameIdentifier>
      <affiliation>Shengjing Hospital of China Medical University</affiliation>
    </creator>
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  <titles>
    <title>
      Data from: TNFAIP9 protects against the development of the early stage of
      chronic kidney disease: Focus on inflammation and fibrosis
    </title>
  </titles>
  <publisher publisherIdentifier="https://ror.org/00x6h5n95" publisherIdentifierScheme="ROR" schemeURI="https://ror.org/">Dryad</publisher>
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    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Chronic kidney disease</subject>
    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Gene therapy</subject>
    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Inflammation</subject>
    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Fibrosis</subject>
  </subjects>
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    <fundingReference>
      <funderName>National Natural Science Foundation of China</funderName>
      <funderIdentifier funderIdentifierType="ROR">https://ror.org/01h0zpd94</funderIdentifier>
      <awardNumber>2021M703608</awardNumber>
    </fundingReference>
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    <date dateType="Created">2024-11-25T07:40:13Z</date>
    <date dateType="Submitted">2024-11-25T07:40:27Z</date>
    <date dateType="Issued">2024-12-31T00:00:00Z</date>
    <date dateType="Available">2024-12-31T00:00:00Z</date>
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  <descriptions>
    <description descriptionType="Abstract">
      Tumor necrosis factor alpha-induced protein 9 (TNFAIP9) is a crucial
      effector molecule that protects cells from inflammatory and metabolic
      damage. This study focuses on investigating the role and regulatory
      mechanisms of TNFAIP9 in the progression of chronic kidney disease (CKD).
      By analyzing CKD-related datasets from the GEO database, we discovered
      that TNFAIP9 was upregulated in CKD patients and CKD mice compared to
      their normal controls. To elucidate the functional role of TNFAIP9, we
      established a mouse model of CKD through a two-step 5/6 nephrectomy (Nx).
      The experimental mice were transduced with an adenoviral vector to express
      TNFAIP9. The results showed that mice undergoing 5/6-Nx developed evident
      renal impairment, inflammation, and fibrosis. Overexpression of TNFAIP9
      resulted in the remission of renal impairment, a decreased inflammatory
      response, and a reduced expression of fibrotic markers. In vitro, human
      renal tubular epithelial human kidney-2 (HK-2) cells were exposed to tumor
      necrosis factor-alpha (TNF-α) or transforming growth factor-beta (TGF-β)
      to simulate inflammatory and fibrotic conditions, respectively. Then, the
      overexpression plasmid or small interfering RNA (siRNA) targeting TNFAIP9
      was transfected into HK-2 cells to either overexpress or knock down the
      target protein. Overexpression of TNFAIP9 reduced the TNF-α-induced
      inflammatory response, while its knockdown amplified it. Likewise,
      overexpression of TNFAIP9 decreased the TGF-β-induced fibrosis, whereas
      its knockdown heightened it. In summary, it is suggested that TNFAIP9
      plays a protective role against the early stage of CKD by suppressing
      renal inflammation and fibrosis. Therefore, targeting TNFAIP9 could be a
      promising therapeutic approach for CKD.
    </description>
    <description descriptionType="Methods">
      &lt;p&gt;The current dataset provides raw data that can be used to form
      the histogram, including quantitative results of
      immunofluorescence/immunohistochemistry/pathology scores, kit results, and
      normalized quantitative results of PCR and Western blot.&lt;/p&gt;
    </description>
    <description descriptionType="TechnicalInfo">
      # Data from: TNFAIP9 protects against the development of the early stage
      of chronic kidney disease: focus on inflammation and fibrosis
      **Introduction** This dataset includes raw data that can be used to form
      the histogram, including quantitative results of
      immunofluorescence/immunohistochemistry/pathology scores, kit results, and
      normalized quantitative results of PCR and Western blot to support the
      study “TNFAIP9 protects against the development of the early stage of
      chronic kidney disease: focus on inflammation and fibrosis”, which is
      currently in press. **File list** Original Data, in pptx format. **Author
      Information** Name: Ying Chen Institution: First Hospital of China Medical
      University, Shenyang, China Co-investigator 1 Name: Yanqiu Li Institution:
      First Hospital of China Medical University, Shenyang, China Corresponding
      author Name: Dr. Deyu Zhang Institution: Shengjing Hospital of China
      Medical University, Shenyang, China **Description of the data and file
      structure** The data presented in this dataset underpins the quantitative
      statistics for each graph featured in the publication, thereby ensuring
      that the dataset is derived from the figures reported in the publication.
      The included data encompasses the following quantitative results: Kit
      tests yielded serum levels of creatinine and urea nitrogen; Masson’s
      trichrome staining quantified the percentage of fibrotic areas relative to
      the total area. ELISA assays determined concentrations of TNF-α, IL-6, and
      MCP-1 within kidney tissue or cell supernatants. Western blot analysis
      provided relative quantification values based on grayscale measurements
      for α-SMA, Fibronectin, TNFAIP9, iNOS, COX-2, p-p65ser536, and Collagen
      III. The percentage of positively stained areas for TNFAIP9, Collagen III,
      and NGAL was assessed through immunohistochemistry. Real-time PCR
      facilitated measurement of relative mRNA content for TNFAIP9. Biochemical
      assays recorded arterial pressure as well as 24-hour urinary protein
      excretion from the animals involved in this study. Hematoxylin-eosin (HE)
      staining offered a quantitative score reflecting tissue injury severity.
      Additionally, immunofluorescence techniques were employed to quantify the
      percentage of positively stained cells. **Acknowledgments** This study was
      funded by the Postdoctoral Science Foundation of China (2021M703608). The
      funders had no role in study design, data collection and analysis,
      decision to publish, or preparation of the manuscript.
    </description>
  </descriptions>
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