{"data":{"id":"10.5061/dryad.kprr4xhc4","type":"dois","attributes":{"doi":"10.5061/dryad.kprr4xhc4","prefix":"10.5061","suffix":"dryad.kprr4xhc4","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Liu, Xiujie","nameType":"Personal","givenName":"Xiujie","familyName":"Liu","affiliation":["University of Nottingham Ningbo China"],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0000-0003-2230-3865","nameIdentifierScheme":"ORCID"}]}],"titles":[{"title":"Gut microbiota-derived gamma-aminobutyric acid from metformin treatment reduces hepatic ischemia/reperfusion injury by inhibiting ferroptosis"}],"publisher":"Dryad","container":{},"publicationYear":2024,"subjects":[{"subject":"FOS: Basic medicine","schemeUri":"https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf","subjectScheme":"fos"},{"subject":"FOS: Basic medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"metformin"},{"subject":"gut microbiota"},{"subject":"ischemia reperfusion injury"}],"contributors":[],"dates":[{"date":"2024-03-11T04:22:06Z","dateType":"Created"},{"date":"2024-03-12T13:20:39Z","dateType":"Submitted"},{"date":"2024-03-29T00:00:00Z","dateType":"Issued"},{"date":"2024-03-29T00:00:00Z","dateType":"Available"}],"language":"en","types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsCitedBy","relatedIdentifier":"10.7554/elife.89045","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["7083955056 bytes"],"formats":[],"version":"6","rightsList":[{"rights":"Creative Commons Zero v1.0 Universal","rightsUri":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc0-1.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Hepatic ischemia/reperfusion injury (HIRI) is a common and inevitable\n factor leading to poor prognosis in various liver diseases, making the\n outcomes of current treatments in the clinic unsatisfactory. Metformin has\n been demonstrated to be beneficial in alleviating HIRI in recent studies,\n however, the underpinning mechanism remains unclear. In this study, we\n found metformin mitigates HIRI-induced ferroptosis through reshaped gut\n microbiota in mice, which was confirmed by the results of fecal microbiota\n transplantation treatment but showed the elimination of the beneficial\n effects when gut bacteria were depleted using antibiotics. Detailedly,\n through 16S rRNA and metagenomic sequencing, we identified that the\n metformin-reshaped microbiota was characterized by the increase of\n gamma-aminobutyric acid (GABA) producing bacteria. This increase was\n further confirmed by the elevation of GABA synthesis key enzymes, glutamic\n acid decarboxylase, and putrescine aminotransferase, in gut microbes of\n metformin-treated mice and healthy volunteers. Furthermore, the benefit of\n GABA against HIRI-induced ferroptosis was demonstrated in GABA-treated\n mice. Collectively, our data indicate that metformin can mitigate\n HIRI-induced ferroptosis by reshaping gut microbiota, with GABA identified\n as a key metabolite.","descriptionType":"Abstract"},{"description":"# Gut microbiota-derived gamma-aminobutyric acid from metformin treatment\n reduces hepatic ischemia/reperfusion injury by inhibiting ferroptosis ##\n I. Dataset Overview This dataset contains the original data used in the\n eLife article, primarily supporting research on hepatic\n ischemia-reperfusion injury. The dataset comprises various types of data\n files, including but not limited to text files, image files, and analysis\n code. # II. File Structure and Naming Convention Data files are organized\n into separate folders based on their location in the manuscript, such as\n \"figure 1 data\", \"figure 2 data\", etc. ## III. Data\n Content Text Data: Contains experimental measurements, statistical\n results, etc. All the data are put in Excel documents named such as\n “original data (Figure 1 data)” in the “Figure 1 data” file, etc. Image\n Data: Includes experimental images, photomicrographs, etc., stored in TIFF\n or JPG format. Acronyms Meaning: ALT and AST refer to glutamic pyruvic\n transaminase and glutamic oxaloacetic transaminase, MDA represents\n Malondialdehyde, and GSH stands for Glutathione. All the information could\n be found in the published paper through the DOI supplied below. IV.\n Contact Information If you encounter any questions or require further\n information while using this dataset, please contact the corresponding\n author via the main text published in eLife (DOI:\n [https://doi.org/10.7554/eLife.89045).](https://doi.org/10.7554/eLife.89045\\).)","descriptionType":"TechnicalInfo"}],"geoLocations":[],"fundingReferences":[],"xml":"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