{"data":{"id":"10.5061/dryad.k0p2ngfm1","type":"dois","attributes":{"doi":"10.5061/dryad.k0p2ngfm1","prefix":"10.5061","suffix":"dryad.k0p2ngfm1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Zhang, Zhao","nameType":"Personal","givenName":"Zhao","familyName":"Zhang","affiliation":["Howard Hughes Medical Institute","University of Wisconsin–Madison"],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0000-0002-1133-4191","nameIdentifierScheme":"ORCID"}]},{"name":"Feng, Zhaomeng","nameType":"Personal","givenName":"Zhaomeng","familyName":"Feng","affiliation":["University of Wisconsin–Madison"],"nameIdentifiers":[]},{"name":"Zhao, Xiaowei","nameType":"Personal","givenName":"Xiaowei","familyName":"Zhao","affiliation":["Howard Hughes Medical Institute"],"nameIdentifiers":[]},{"name":"Yu, Zhiheng","nameType":"Personal","givenName":"Zhiheng","familyName":"Yu","affiliation":["Howard Hughes Medical Institute"],"nameIdentifiers":[]},{"name":"Chapman, Edwin","nameType":"Personal","givenName":"Edwin","familyName":"Chapman","affiliation":["Howard Hughes Medical Institute","University of Wisconsin–Madison"],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0000-0001-9787-8140","nameIdentifierScheme":"ORCID"}]}],"titles":[{"title":"Programmable liposome organization via DNA origami templates"}],"publisher":"Dryad","container":{},"publicationYear":2025,"subjects":[{"subject":"FOS: Nano-technology","schemeUri":"https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf","subjectScheme":"fos"},{"subject":"FOS: Chemical sciences","schemeUri":"https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf","subjectScheme":"fos"},{"subject":"FOS: Chemical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","schemeUri":"https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf","subjectScheme":"fos"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"dna origami"},{"subject":"Liposomes","schemeUri":"https://github.com/PLOS/plos-thesaurus","subjectScheme":"PLOS Subject Area Thesaurus"},{"subject":"DNA nanotechnology","schemeUri":"https://github.com/PLOS/plos-thesaurus","subjectScheme":"PLOS Subject Area Thesaurus"},{"subject":"Nano-enabled synthetic biology","schemeUri":"https://github.com/PLOS/plos-thesaurus","subjectScheme":"PLOS Subject Area Thesaurus"}],"contributors":[],"dates":[{"date":"2025-08-08T22:05:48Z","dateType":"Created"},{"date":"2025-08-08T22:08:11Z","dateType":"Submitted"},{"date":"2025-09-05T00:00:00Z","dateType":"Issued"},{"date":"2025-09-05T00:00:00Z","dateType":"Available"}],"language":"en","types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsCitedBy","relatedIdentifier":"10.1021/jacs.5c05196","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["2194929152 bytes"],"formats":[],"version":"2","rightsList":[{"rights":"Creative Commons Zero v1.0 Universal","rightsUri":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc0-1.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Liposomes are essential vehicles for membrane protein reconstitution and\n drug delivery, making them vital tools in both in vivo and in vitro\n studies. However, the lack of robust techniques for the precise\n arrangement of these synthetic vesicles limits their potential\n applications. Here, we present a modular polymerization platform based on\n square DNA origami to template the formation and organization of\n liposomes. By programming the sequence, number, position, chirality, and\n flexibility of sticky ends on each square, we assemble uniformly sized\n liposomes into diverse two-dimensional (2D) arrays, as well as finite\n lattices and rings. Additionally, we demonstrate stepwise assembly and\n targeted disassembly, enabling dynamic structural control. These complex\n liposome architectures represent a significant advancement in the fields\n of biotechnology, nanotechnology, and bottom-up biology.","descriptionType":"Abstract"},{"description":"# Programmable liposome organization via DNA origami templates Dataset\n DOI: [10.5061/dryad.k0p2ngfm1](10.5061/dryad.k0p2ngfm1) ## Description of\n the data and file structure Square DNA origami (SDO) was designed using\n **cadnano**, assembled with an M13-derived scaffold, and purified via\n glycerol gradient centrifugation. **SDO-templated liposomes (STLs)** were\n formed by hybridizing cholesterol-labeled oligos to SDO, followed by lipid\n rehydration, mixing, dialysis, and iodixanol gradient purification.\n Polymerization was controlled by thermal annealing, and dynamic behavior\n was modulated using strand displacement. Samples were analyzed using\n **negative-stain TEM**, **cryo-EM**, and **gel electrophoresis** to assess\n structure, assembly, and polymer formation. Cryo-EM data were acquired on\n a Titan Krios and processed to correct for beam-induced motion. ### Files\n and variables #### File: TEM_images.zip **Description:** This dataset\n contains a curated set of representative negative-stain transmission\n electron microscopy (TEM) images of square DNA origami (SDO) structures\n and SDO-templated liposomes (STLs). Each image provides visual\n documentation of the nanostructures' morphology and assembly quality.\n The image files are named systematically using the following format:\n Structure type: either SDO or STL Description: a brief label describing\n the configuration or experimental condition (e.g., “zigzag array v2”)\n Serial number: a two-digit identifier for indexing purposes (e.g., “03”)\n ## Access information The corresponding figure numbers in the main text\n and Supporting Information are indexed below. \\* 1a: SDO_Monomer \\* 1b:\n STL_Monomer_fraction5 \\* 1c \u0026amp; S4: STL_2D_array; STL_2D_array_TMSD \\*\n 1d \u0026amp; S6: STL_1D_array; SDO_1D_array \\* 1e \u0026amp; S7: STL_3layer_array;\n SDO_3layer_array \\* 1f \u0026amp; S8: STL_Zigzag_array_v1; SDO_Zigzag_array_v1\n \\* 1g \u0026amp; S9: STL_Zigzag_array_v2; SDO_Zigzag_array_v2 \\* 1h \u0026amp; S10:\n STL_4mer_v1; SDO_4mer_v1 \\* 1i \u0026amp; S11: STL_8mer_v1; SDO_8mer_v1 \\* 2a\n \u0026amp; S14 \u0026amp; S18: STL_5mer_v1; SDO_5mer_v1; STL_9mer_v1; SDO_9mer_v1 \\*\n 2b \u0026amp; S15 \u0026amp; S19: STL_4mer_v2; SDO_4mer_v2; STL_7mer_v1; SDO_7mer_v1\n \\* 2c \u0026amp; S16 \u0026amp; S20: STL_3mer_v1; SDO_3mer_v1; STL_5mer_v2;\n SDO_5mer_v2 \\* 2d \u0026amp; S17 \u0026amp; S21: STL_3mer_v2; SDO_3mer_v2;\n STL_5mer_v3; SDO_5mer_v3 \\* 2e \u0026amp; S22: STL_4mer_v3; SDO_4mer_v3 \\* 2f\n \u0026amp; S23: STL_4mer_v4; SDO_4mer_v4 \\* 2g \u0026amp; S24: STL_5mer_v4;\n SDO_5mer_v4 \\* 2h \u0026amp; S25: STL_6mer_v1; SDO_6mer_v1 \\* 2i \u0026amp; S26:\n STL_6mer_v2; SDO_6mer_v2 \\* 2j \u0026amp; S27: STL_8mer_v2 \\* 2k \u0026amp; S28:\n STL_9mer_v2; SDO_9mer_v2 \\* 2l \u0026amp; S29: STL_7mer_v2; SDO_7mer_v2 \\* 2m\n \u0026amp; S30: STL_5mer_v5; SDO_5mer_v5 \\* 2n \u0026amp; S31: STL_5mer_v6;\n SDO_5mer_v6 \\* 2o \u0026amp; S32: STL_5mer_v7; SDO_5mer_v7 \\* 2p \u0026amp; S33:\n STL_5mer_v8; SDO_5mer_v8 \\* 2q \u0026amp; S34: STL_7mer_v3 \\* 2r \u0026amp; S35:\n STL_6mer_v3; SDO_6mer_v3 \\* 2s \u0026amp; S36: STL_6mer_v4; SDO_6mer_v4 \\* 2t\n \u0026amp; S34: STL_8mer_v3 \\* 2u \u0026amp; S37: STL_7mer_v4; SDO_7mer_v4 \\* 2v\n \u0026amp; S38: STL_9mer_v3; SDO_9mer_v3 \\* 2w \u0026amp; S39: STL_6mer_v5;\n SDO_6mer_v5 \\* 2x \u0026amp; S40-S43: STL_hetero_3mer; STL_hetero_4mer;\n STL_hetero_5mer; STL_hetero_6mer; SDO_hetero_3mer; SDO_hetero_4mer;\n SDO_hetero_5mer; SDO_hetero_6mer \\* 3a \u0026amp; S44:\n STL_stepwise_assembly_2mer; STL_stepwise_assembly_3mer;\n STL_stepwise_assembly_4mer \\* 3b \u0026amp; S45: STL_dynamic_disassembly;\n SDO_dynamic_disassembly \\* S2: SDO_2D_array \\* S3: STL_Monomer_fraction3;\n STL_Monomer_fraction4; STL_Monomer_fraction5; STL_Monomer_fraction6 \\*\n S12: STL_4mer_v1_RT3h; STL_4mer_v1_4C15h \\* S13: SDO_2mer; STL_2mer","descriptionType":"TechnicalInfo"}],"geoLocations":[],"fundingReferences":[{"schemeUri":"https://ror.org","funderName":"Howard Hughes Medical Institute","funderIdentifier":"https://ror.org/006w34k90","funderIdentifierType":"ROR"},{"schemeUri":"https://ror.org","funderName":"National Institute of Mental Health","awardNumber":"MH061876","funderIdentifier":"https://ror.org/04xeg9z08","funderIdentifierType":"ROR"},{"schemeUri":"https://ror.org","funderName":"National Institute of Neurological Disorders and Stroke","awardNumber":"NS136306","funderIdentifier":"https://ror.org/01s5ya894","funderIdentifierType":"ROR"}],"xml":"<?xml version="1.0" encoding="UTF-8"?>
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  <identifier identifierType="DOI">10.5061/DRYAD.K0P2NGFM1</identifier>
  <creators>
    <creator>
      <creatorName nameType="Personal">Zhang, Zhao</creatorName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0002-1133-4191</nameIdentifier>
      <affiliation affiliationIdentifier="https://ror.org/006w34k90" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Howard Hughes Medical Institute</affiliation>
      <affiliation affiliationIdentifier="https://ror.org/01y2jtd41" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of Wisconsin–Madison</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Feng, Zhaomeng</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/01y2jtd41" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of Wisconsin–Madison</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Zhao, Xiaowei</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/006w34k90" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Howard Hughes Medical Institute</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Yu, Zhiheng</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/006w34k90" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Howard Hughes Medical Institute</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Chapman, Edwin</creatorName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0001-9787-8140</nameIdentifier>
      <affiliation affiliationIdentifier="https://ror.org/006w34k90" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Howard Hughes Medical Institute</affiliation>
      <affiliation affiliationIdentifier="https://ror.org/01y2jtd41" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of Wisconsin–Madison</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Programmable liposome organization via DNA origami templates</title>
  </titles>
  <publisher publisherIdentifier="https://ror.org/00x6h5n95" publisherIdentifierScheme="ROR" schemeURI="https://ror.org/">Dryad</publisher>
  <resourceType resourceTypeGeneral="Dataset">dataset</resourceType>
  <publicationYear>2025</publicationYear>
  <subjects>
    <subject subjectScheme="fos" schemeURI="https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf">FOS: Nano-technology</subject>
    <subject subjectScheme="fos" schemeURI="https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf">FOS: Chemical sciences</subject>
    <subject subjectScheme="fos" schemeURI="https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf">FOS: Biological sciences</subject>
    <subject>dna origami</subject>
    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Liposomes</subject>
    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">DNA nanotechnology</subject>
    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Nano-enabled synthetic biology</subject>
  </subjects>
  <fundingReferences>
    <fundingReference>
      <funderName>Howard Hughes Medical Institute</funderName>
      <funderIdentifier funderIdentifierType="ROR">https://ror.org/006w34k90</funderIdentifier>
    </fundingReference>
    <fundingReference>
      <funderName>National Institute of Mental Health</funderName>
      <funderIdentifier funderIdentifierType="ROR">https://ror.org/04xeg9z08</funderIdentifier>
      <awardNumber>MH061876</awardNumber>
    </fundingReference>
    <fundingReference>
      <funderName>National Institute of Neurological Disorders and Stroke</funderName>
      <funderIdentifier funderIdentifierType="ROR">https://ror.org/01s5ya894</funderIdentifier>
      <awardNumber>NS136306</awardNumber>
    </fundingReference>
  </fundingReferences>
  <dates>
    <date dateType="Created">2025-08-08T22:05:48Z</date>
    <date dateType="Submitted">2025-08-08T22:08:11Z</date>
    <date dateType="Issued">2025-09-05T00:00:00Z</date>
    <date dateType="Available">2025-09-05T00:00:00Z</date>
  </dates>
  <language>en</language>
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    <rights rightsURI="https://spdx.org/licenses/CC0-1.0.html">Creative Commons Zero v1.0 Universal</rights>
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  <descriptions>
    <description descriptionType="Abstract">
      Liposomes are essential vehicles for membrane protein reconstitution and
      drug delivery, making them vital tools in both in vivo and in vitro
      studies. However, the lack of robust techniques for the precise
      arrangement of these synthetic vesicles limits their potential
      applications. Here, we present a modular polymerization platform based on
      square DNA origami to template the formation and organization of
      liposomes. By programming the sequence, number, position, chirality, and
      flexibility of sticky ends on each square, we assemble uniformly sized
      liposomes into diverse two-dimensional (2D) arrays, as well as finite
      lattices and rings. Additionally, we demonstrate stepwise assembly and
      targeted disassembly, enabling dynamic structural control. These complex
      liposome architectures represent a significant advancement in the fields
      of biotechnology, nanotechnology, and bottom-up biology.
    </description>
    <description descriptionType="TechnicalInfo">
      # Programmable liposome organization via DNA origami templates Dataset
      DOI: [10.5061/dryad.k0p2ngfm1](10.5061/dryad.k0p2ngfm1) ## Description of
      the data and file structure Square DNA origami (SDO) was designed using
      **cadnano**, assembled with an M13-derived scaffold, and purified via
      glycerol gradient centrifugation. **SDO-templated liposomes (STLs)** were
      formed by hybridizing cholesterol-labeled oligos to SDO, followed by lipid
      rehydration, mixing, dialysis, and iodixanol gradient purification.
      Polymerization was controlled by thermal annealing, and dynamic behavior
      was modulated using strand displacement. Samples were analyzed using
      **negative-stain TEM**, **cryo-EM**, and **gel electrophoresis** to assess
      structure, assembly, and polymer formation. Cryo-EM data were acquired on
      a Titan Krios and processed to correct for beam-induced motion. ### Files
      and variables #### File: TEM_images.zip **Description:** This dataset
      contains a curated set of representative negative-stain transmission
      electron microscopy (TEM) images of square DNA origami (SDO) structures
      and SDO-templated liposomes (STLs). Each image provides visual
      documentation of the nanostructures' morphology and assembly quality.
      The image files are named systematically using the following format:
      Structure type: either SDO or STL Description: a brief label describing
      the configuration or experimental condition (e.g., “zigzag array v2”)
      Serial number: a two-digit identifier for indexing purposes (e.g., “03”)
      ## Access information The corresponding figure numbers in the main text
      and Supporting Information are indexed below. \* 1a: SDO_Monomer \* 1b:
      STL_Monomer_fraction5 \* 1c &amp; S4: STL_2D_array; STL_2D_array_TMSD \*
      1d &amp; S6: STL_1D_array; SDO_1D_array \* 1e &amp; S7: STL_3layer_array;
      SDO_3layer_array \* 1f &amp; S8: STL_Zigzag_array_v1; SDO_Zigzag_array_v1
      \* 1g &amp; S9: STL_Zigzag_array_v2; SDO_Zigzag_array_v2 \* 1h &amp; S10:
      STL_4mer_v1; SDO_4mer_v1 \* 1i &amp; S11: STL_8mer_v1; SDO_8mer_v1 \* 2a
      &amp; S14 &amp; S18: STL_5mer_v1; SDO_5mer_v1; STL_9mer_v1; SDO_9mer_v1 \*
      2b &amp; S15 &amp; S19: STL_4mer_v2; SDO_4mer_v2; STL_7mer_v1; SDO_7mer_v1
      \* 2c &amp; S16 &amp; S20: STL_3mer_v1; SDO_3mer_v1; STL_5mer_v2;
      SDO_5mer_v2 \* 2d &amp; S17 &amp; S21: STL_3mer_v2; SDO_3mer_v2;
      STL_5mer_v3; SDO_5mer_v3 \* 2e &amp; S22: STL_4mer_v3; SDO_4mer_v3 \* 2f
      &amp; S23: STL_4mer_v4; SDO_4mer_v4 \* 2g &amp; S24: STL_5mer_v4;
      SDO_5mer_v4 \* 2h &amp; S25: STL_6mer_v1; SDO_6mer_v1 \* 2i &amp; S26:
      STL_6mer_v2; SDO_6mer_v2 \* 2j &amp; S27: STL_8mer_v2 \* 2k &amp; S28:
      STL_9mer_v2; SDO_9mer_v2 \* 2l &amp; S29: STL_7mer_v2; SDO_7mer_v2 \* 2m
      &amp; S30: STL_5mer_v5; SDO_5mer_v5 \* 2n &amp; S31: STL_5mer_v6;
      SDO_5mer_v6 \* 2o &amp; S32: STL_5mer_v7; SDO_5mer_v7 \* 2p &amp; S33:
      STL_5mer_v8; SDO_5mer_v8 \* 2q &amp; S34: STL_7mer_v3 \* 2r &amp; S35:
      STL_6mer_v3; SDO_6mer_v3 \* 2s &amp; S36: STL_6mer_v4; SDO_6mer_v4 \* 2t
      &amp; S34: STL_8mer_v3 \* 2u &amp; S37: STL_7mer_v4; SDO_7mer_v4 \* 2v
      &amp; S38: STL_9mer_v3; SDO_9mer_v3 \* 2w &amp; S39: STL_6mer_v5;
      SDO_6mer_v5 \* 2x &amp; S40-S43: STL_hetero_3mer; STL_hetero_4mer;
      STL_hetero_5mer; STL_hetero_6mer; SDO_hetero_3mer; SDO_hetero_4mer;
      SDO_hetero_5mer; SDO_hetero_6mer \* 3a &amp; S44:
      STL_stepwise_assembly_2mer; STL_stepwise_assembly_3mer;
      STL_stepwise_assembly_4mer \* 3b &amp; S45: STL_dynamic_disassembly;
      SDO_dynamic_disassembly \* S2: SDO_2D_array \* S3: STL_Monomer_fraction3;
      STL_Monomer_fraction4; STL_Monomer_fraction5; STL_Monomer_fraction6 \*
      S12: STL_4mer_v1_RT3h; STL_4mer_v1_4C15h \* S13: SDO_2mer; STL_2mer
    </description>
  </descriptions>
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