{"data":{"id":"10.5061/dryad.j0zpc86ht","type":"dois","attributes":{"doi":"10.5061/dryad.j0zpc86ht","prefix":"10.5061","suffix":"dryad.j0zpc86ht","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Qi, Houbao","nameType":"Personal","givenName":"Houbao","familyName":"Qi","affiliation":["Binzhou Medical University"],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0000-0002-5451-1370","nameIdentifierScheme":"ORCID"}]},{"name":"Li, Yuntao","nameType":"Personal","givenName":"Yuntao","familyName":"Li","affiliation":["Binzhou Medical University"],"nameIdentifiers":[]},{"name":"Liu, Xianbing","nameType":"Personal","givenName":"Xianbing","familyName":"Liu","affiliation":["Binzhou Medical University"],"nameIdentifiers":[]},{"name":"Hu, Xuemei","nameType":"Personal","givenName":"Xuemei","familyName":"Hu","affiliation":["Binzhou Medical University"],"nameIdentifiers":[]}],"titles":[{"title":"Tim-3 regulates the immunosuppressive function of decidual MDSCs via the Fyn-STAT3-C/EBPβ pathway during Toxoplasma gondii infection"}],"publisher":"Dryad","container":{},"publicationYear":2023,"subjects":[{"subject":"FOS: Basic medicine","schemeUri":"https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf","subjectScheme":"fos"},{"subject":"FOS: Basic medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2023-05-26T12:33:45Z","dateType":"Submitted"},{"date":"2023-06-08T00:00:00Z","dateType":"Issued"},{"date":"2023-06-08T00:00:00Z","dateType":"Available"}],"language":"en","types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsCitedBy","relatedIdentifier":"10.1371/journal.ppat.1011329","relatedIdentifierType":"DOI"},{"relationType":"IsSourceOf","relatedIdentifier":"10.5281/zenodo.7535958","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["2216655331 bytes"],"formats":[],"version":"5","rightsList":[{"rights":"Creative Commons Zero v1.0 Universal","rightsUri":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc0-1.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Myeloid-derived suppressor cells (MDSCs) play a key role in maintaining\n maternal-fetal tolerance for a successful pregnancy, but the role of MDSCs\n in abnormal pregnancy caused by Toxoplasma gondii infection is\n unknown. Herein, we revealed a distinct mechanism by which T-cell\n immunoglobulin domain- and mucin domain-containing protein-3 (Tim-3), an\n immune checkpoint receptor that balances maternal-fetal tolerance during\n pregnancy, contributes to the immunosuppressive function of MDSCs during\n T. gondii infection. The expression of Tim-3 in decidual MDSCs was\n significantly downregulated following T. gondii infection. The proportion\n of monocytic MDSC population, the inhibitory effect of MDSCs on T-cell\n proliferation, the levels of STAT3 phosphorylation, and the expression of\n functional molecules (Arg-1 and IL-10) in MDSCs were all decreased in T.\n gondii-infected pregnant Tim-3 gene knockout (Tim-3KO) mice compared with\n infected pregnant WT mice. After treatment with Tim-3-neutralizing Ab in\n vitro, the expression levels of Arg-1, IL-10, C/EBPβ, and p-STAT3 were\n decreased, the interaction between Fyn and Tim-3 or between Fyn and STAT3\n was weakened, and the binding ability of C/EBPβ to the promoters of ARG1\n and IL10 was decreased in human decidual MDSCs with T. gondii infection,\n while opposite results were observed following treatment with galectin-9\n (a ligand for Tim-3). Inhibitors of Fyn and STAT3 also downregulated the\n expression of Arg-1 and IL-10 in decidual MDSCs and exacerbated adverse\n pregnancy outcomes caused by T. gondii infection in mice. Therefore, our\n studies discovered that the decrease of Tim-3 after T. gondii infection\n could downregulate the functional molecules of Arg-1 and IL-10 expression\n in decidual MDSCs through the Fyn-STAT3-C/EBPβ signaling pathway and\n weaken their immunosuppressive function, which eventually contributes to\n the development of adverse pregnancy outcomes.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[{"schemeUri":"https://ror.org","funderName":"National Natural Science Foundation of China","funderIdentifier":"https://ror.org/01h0zpd94","funderIdentifierType":"ROR"}],"xml":"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