{"data":{"id":"10.5061/dryad.hqbzkh1mt","type":"dois","attributes":{"doi":"10.5061/dryad.hqbzkh1mt","prefix":"10.5061","suffix":"dryad.hqbzkh1mt","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Efford, Meaghan","nameType":"Personal","givenName":"Meaghan","familyName":"Efford","affiliation":["University of British Columbia"],"nameIdentifiers":[]},{"name":"Taft, Spencer","nameType":"Personal","givenName":"Spencer","familyName":"Taft","affiliation":["Tsleil-Waututh Nation"],"nameIdentifiers":[]},{"name":"George, Micheal","nameType":"Personal","givenName":"Micheal","familyName":"George","affiliation":["Tsleil-Waututh Nation"],"nameIdentifiers":[]},{"name":"George, Michelle","nameType":"Personal","givenName":"Michelle","familyName":"George","affiliation":["Tsleil-Waututh Nation"],"nameIdentifiers":[]},{"name":"Morin, Jesse","nameType":"Personal","givenName":"Jesse","familyName":"Morin","affiliation":["University of British Columbia"],"nameIdentifiers":[]},{"name":"Cavers, Hannah","nameType":"Personal","givenName":"Hannah","familyName":"Cavers","affiliation":["University of British Columbia"],"nameIdentifiers":[]},{"name":"Hilsden, Jay","nameType":"Personal","givenName":"Jay","familyName":"Hilsden","affiliation":["University of British Columbia"],"nameIdentifiers":[]},{"name":"Paskulin, Lindsey","nameType":"Personal","givenName":"Lindsey","familyName":"Paskulin","affiliation":["University of British Columbia"],"nameIdentifiers":[]},{"name":"Loewen, Doris","nameType":"Personal","givenName":"Doris","familyName":"Loewen","affiliation":["University of British Columbia"],"nameIdentifiers":[]},{"name":"Zhu, Jennifer","nameType":"Personal","givenName":"Jennifer","familyName":"Zhu","affiliation":["University of British Columbia"],"nameIdentifiers":[]},{"name":"Christensen, Villy","nameType":"Personal","givenName":"Villy","familyName":"Christensen","affiliation":["University of British Columbia"],"nameIdentifiers":[]},{"name":"Speller, Camilla","nameType":"Personal","givenName":"Camilla","familyName":"Speller","affiliation":["University of British Columbia"],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0000-0001-7128-9903","nameIdentifierScheme":"ORCID"}]}],"titles":[{"title":"Archaeology demonstrates sustainable Ancestral Coast Salish salmon stewardship over thousands of years pre-contact"}],"publisher":"Dryad","container":{},"publicationYear":2023,"subjects":[{"subject":"ZooMS"},{"subject":"Pacific Salmon"},{"subject":"Archaeology","schemeUri":"https://github.com/PLOS/plos-thesaurus","subjectScheme":"PLOS Subject Area Thesaurus"},{"subject":"Tsleil-Waututh First Nation"},{"subject":"Coast Salish"},{"subject":"Ecosystem Management"},{"subject":"FOS: History and archaeology","schemeUri":"https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf","subjectScheme":"fos"},{"subject":"FOS: History and archaeology","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[{"name":"DNA, Ancient","nameType":"Personal","givenName":"Ancient","familyName":"DNA","affiliation":[],"contributorType":"Sponsor","nameIdentifiers":[]}],"dates":[{"date":"2023-09-13T00:04:21Z","dateType":"Created"},{"date":"2023-09-07T10:24:53Z","dateType":"Submitted"},{"date":"2023-09-12T00:00:00Z","dateType":"Issued"},{"date":"2023-09-12T00:00:00Z","dateType":"Available"}],"language":"en","types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsCitedBy","relatedIdentifier":"10.1371/journal.pone.0289797","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["1146246169 bytes"],"formats":[],"version":"3","rightsList":[{"rights":"Creative Commons Zero v1.0 Universal","rightsUri":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc0-1.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Salmon are an essential component of the ecosystem in Tsleil-Waututh\n Nation’s traditional, ancestral, and unceded territory, centred on\n present-day Burrard Inlet, BC, Canada, where Tsleil-Waututh people have\n been harvesting salmon, along with a wide variety of other fishes, for\n millennia. Tsleil-Waututh Nation is an ancestral Coast Salish community\n that has called the Inlet home since time immemorial. This research\n assesses the continuity and sustainability of the salmon fishery at\n təmtəmíxʷtən, an ancestral Tsleil-Waututh settlement in the Inlet, over\n thousands of years before European contact (1792 CE). We apply\n Zooarchaeology by Mass Spectrometry (ZooMS) analysis to 245 archaeological\n salmon vertebrae to identify the species that were harvested by the\n Tsleil-Waututh community that lived at təmtəmíxʷtən. The results\n demonstrate that Tsleil-Waututh communities consistently and\n preferentially fished for chum salmon (Oncorhynchus keta) over the period\n of almost 3,000 years. The consistent abundance indicates a sustainable\n chum salmon fishery over that time and a strong salmon-to-people\n relationship through generations. This research supports Tsleil-Waututh\n Nation’s stewardship obligations under their ancestral legal principles to\n maintain conditions that uphold the Nation’s way of life.","descriptionType":"Abstract"},{"description":"All samples were analysed\n within the Ancient DNA and Proteins (ADαPT) Facility in the Department of\n Anthropology at the University of British Columbia. A total of 245 fish\n vertebrae were analysed using the method published in Buckley et al. 2009,\n modified as described in Richter et al. Briefly, ca. 10–30 mg of bone was\n subsampled and demineralized 0.6 M HCl at 4°C. Samples were rinsed in 200\n μL of 0.1 M NaOH to remove humic compound, then rinsed three times in the\n same volume of 50 mM ammonium bicarbonate solution (NH4HCO3) pH 8.0\n (AmBic). Samples were gelatinized through incubation in 100 μL of AmBic at\n 65 °C for 1 h at 65, before being enzymatically digested overnight at 37\n °C in 0.4 μg of trypsin. Digested samples were acidified to 0.1%\n trifluoroacetic acid (TFA) and purified using Pierce™ 100 μL C18 tips\n (ThermoFisher). One microliter of α-cyano-hydroxycinnamic acid (matrix)\n was added to 1 μL of collagen extract and spotted in triplicate with onto\n a 384 spot MALDI target plate alongside calibration standards. MALDI-TOF\n was conducted on a Bruker Ultraflex III mass spectrometer with a Nd:YAG\n smart beam laser, with a SNAP averaging algorithm used to obtain\n monoisotopic masses (C 4.9384, N 1.3577, O 1.4773, S 0.0417, H 7.7583).\n Triplicate spectra were averaged and visually inspected using mMass\n software to identify diagnostic markers published in Richter et al. The\n raw MALDI spectra are uploaded here. ","descriptionType":"Methods"},{"description":"# Archaeology demonstrates sustainable Ancestral Coast Salish salmon\n stewardship over thousands of years pre-contact: MALDI-TOF data --- Salmon\n are an essential component of the ecosystem in Tsleil-Waututh Nation’s\n traditional, ancestral, and unceded territory, centred on present-day\n Burrard Inlet, BC, Canada, where Tsleil-Waututh people have been\n harvesting salmon, along with a wide variety of other fishes, for\n millennia. Tsleil-Waututh Nation is an ancestral Coast Salish community\n that has called the Inlet home since time immemorial. This research\n assesses the continuity and sustainability of the salmon fishery at\n təmtəmíxʷtən, an ancestral Tsleil-Waututh settlement in the Inlet, over\n thousands of years before European contact (1792 CE). We apply\n Zooarchaeology by Mass Spectrometry (ZooMS) analysis to 245 archaeological\n salmon vertebrae to identify the species that were harvested by the\n Tsleil-Waututh community that lived at təmtəmíxʷtən. The results\n demonstrate that Tsleil-Waututh communities consistently and\n preferentially fished for chum salmon (*Oncorhynchus keta*) over the\n period of almost 3,000 years. The consistent abundance indicates a\n sustainable chum salmon fishery over that time and a strong\n salmon-to-people relationship through generations. This research supports\n Tsleil-Waututh Nation’s stewardship obligations under their ancestral\n legal principles to maintain conditions that uphold the Nation’s way of\n life. ## Description of the Data and file structure This zip file contains\n raw MALDI-TOF spectra for each of the 245 salmonid vertebrae sampled for\n ZooMS. Each sample was spotted in triplicate, along with calibration\n standards, onto a 384 spot Bruker MALDI ground steel target plate using 1\n µL of sample and 1 µL of α-cyano-hydroxycinnamic acid matrix. The samples\n were run on a Bruker ultraflex III MALDI TOF/TOF mass spectrometer with a\n Nd:YAG smart beam laser University of York in York, UK, and a SNAP\n averaging algorithm was used to obtain monoisotopic masses (C 4.9384, N\n 1.3577, O 1.4773, S 0.0417, H 7.7583). Raw spectral data has been uploaded\n here for each sample in triplicate. Each sample is within an individual\n folder with the sample name (e.g., A713). Each raw spectrum is labelled\n with the sample name and the spot location on the MALDI Plate (e.g.,\n _A1,_A4, _A9). ## Sharing/access Information Links to other publicly\n accessible locations of the data: None.","descriptionType":"TechnicalInfo"}],"geoLocations":[],"fundingReferences":[{"schemeUri":"https://ror.org","funderName":"Natural Sciences and Engineering Research Council","awardNumber":"CREATE Program","funderIdentifier":"https://ror.org/01h531d29","funderIdentifierType":"ROR"},{"schemeUri":"https://ror.org","funderName":"Natural Sciences and Engineering Research Council","awardNumber":"DGECR-2019-00426","funderIdentifier":"https://ror.org/01h531d29","funderIdentifierType":"ROR"},{"schemeUri":"https://ror.org","funderName":"Mitacs","awardNumber":"Accelerate Program","funderIdentifier":"https://ror.org/00cjrc276","funderIdentifierType":"ROR"}],"xml":"<?xml version="1.0" encoding="UTF-8"?>
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    <creator>
      <creatorName nameType="Personal">Efford, Meaghan</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/03rmrcq20" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of British Columbia</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Taft, Spencer</creatorName>
      <affiliation>Tsleil-Waututh Nation</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">George, Micheal</creatorName>
      <affiliation>Tsleil-Waututh Nation</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">George, Michelle</creatorName>
      <affiliation>Tsleil-Waututh Nation</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Morin, Jesse</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/03rmrcq20" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of British Columbia</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Cavers, Hannah</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/03rmrcq20" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of British Columbia</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Hilsden, Jay</creatorName>
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    <creator>
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    <creator>
      <creatorName nameType="Personal">Loewen, Doris</creatorName>
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    </creator>
    <creator>
      <creatorName nameType="Personal">Zhu, Jennifer</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/03rmrcq20" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of British Columbia</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Christensen, Villy</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/03rmrcq20" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of British Columbia</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Speller, Camilla</creatorName>
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      <affiliation affiliationIdentifier="https://ror.org/03rmrcq20" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of British Columbia</affiliation>
    </creator>
  </creators>
  <titles>
    <title>
      Archaeology demonstrates sustainable Ancestral Coast Salish salmon
      stewardship over thousands of years pre-contact
    </title>
  </titles>
  <publisher publisherIdentifier="https://ror.org/00x6h5n95" publisherIdentifierScheme="ROR" schemeURI="https://ror.org/">Dryad</publisher>
  <resourceType resourceTypeGeneral="Dataset">dataset</resourceType>
  <publicationYear>2023</publicationYear>
  <subjects>
    <subject>ZooMS</subject>
    <subject>Pacific Salmon</subject>
    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Archaeology</subject>
    <subject>Tsleil-Waututh First Nation</subject>
    <subject>Coast Salish</subject>
    <subject>Ecosystem Management</subject>
    <subject subjectScheme="fos" schemeURI="https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf">FOS: History and archaeology</subject>
  </subjects>
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      <funderIdentifier funderIdentifierType="ROR">https://ror.org/01h531d29</funderIdentifier>
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      <awardNumber>DGECR-2019-00426</awardNumber>
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      <funderName>Mitacs</funderName>
      <funderIdentifier funderIdentifierType="ROR">https://ror.org/00cjrc276</funderIdentifier>
      <awardNumber>Accelerate Program</awardNumber>
    </fundingReference>
  </fundingReferences>
  <contributors>
    <contributor contributorType="Sponsor">
      <contributorName>Ancient DNA and Proteins Facility, UBC*</contributorName>
    </contributor>
  </contributors>
  <dates>
    <date dateType="Created">2023-09-13T00:04:21Z</date>
    <date dateType="Submitted">2023-09-07T10:24:53Z</date>
    <date dateType="Issued">2023-09-12T00:00:00Z</date>
    <date dateType="Available">2023-09-12T00:00:00Z</date>
  </dates>
  <language>en</language>
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  <descriptions>
    <description descriptionType="Abstract">
      Salmon are an essential component of the ecosystem in Tsleil-Waututh
      Nation’s traditional, ancestral, and unceded territory, centred on
      present-day Burrard Inlet, BC, Canada, where Tsleil-Waututh people have
      been harvesting salmon, along with a wide variety of other fishes, for
      millennia. Tsleil-Waututh Nation is an ancestral Coast Salish community
      that has called the Inlet home since time immemorial. This research
      assesses the continuity and sustainability of the salmon fishery at
      təmtəmíxʷtən, an ancestral Tsleil-Waututh settlement in the Inlet, over
      thousands of years before European contact (1792 CE). We apply
      Zooarchaeology by Mass Spectrometry (ZooMS) analysis to 245 archaeological
      salmon vertebrae to identify the species that were harvested by the
      Tsleil-Waututh community that lived at təmtəmíxʷtən. The results
      demonstrate that Tsleil-Waututh communities consistently and
      preferentially fished for chum salmon (Oncorhynchus keta) over the period
      of almost 3,000 years. The consistent abundance indicates a sustainable
      chum salmon fishery over that time and a strong salmon-to-people
      relationship through generations. This research supports Tsleil-Waututh
      Nation’s stewardship obligations under their ancestral legal principles to
      maintain conditions that uphold the Nation’s way of life.
    </description>
    <description descriptionType="Methods">
      &lt;p&gt;&lt;span lang="EN-CA"&gt;All samples were analysed
      within the Ancient DNA and Proteins (ADαPT) Facility in the Department of
      Anthropology at the University of British Columbia. A total of 245 fish
      vertebrae were analysed using the method published in Buckley et al. 2009,
      modified as described in Richter et al. Briefly, ca. 10–30 mg of bone was
      subsampled and demineralized 0.6 M HCl at 4°C. Samples were rinsed in 200
      μL of 0.1 M NaOH to remove humic compound, then rinsed three times in the
      same volume of 50 mM ammonium bicarbonate solution (NH4HCO3) pH 8.0
      (AmBic). Samples were gelatinized through incubation in 100 μL of AmBic at
      65 °C for 1 h at 65, before being enzymatically digested overnight at 37
      °C in 0.4 μg of trypsin. Digested samples were acidified to 0.1%
      trifluoroacetic acid (TFA) and purified using Pierce™ 100 μL C18 tips
      (ThermoFisher). One microliter of α-cyano-hydroxycinnamic acid (matrix)
      was added to 1 μL of collagen extract and spotted in triplicate with onto
      a 384 spot MALDI target plate alongside calibration standards. MALDI-TOF
      was conducted on a Bruker Ultraflex III mass spectrometer with a Nd:YAG
      smart beam laser, with a SNAP averaging algorithm used to obtain
      monoisotopic masses (C 4.9384, N 1.3577, O 1.4773, S 0.0417, H 7.7583).
      Triplicate spectra were averaged and visually inspected using mMass
      software to identify diagnostic markers published in Richter et al. The
      raw MALDI spectra are uploaded here. &lt;/span&gt;&lt;/p&gt;
    </description>
    <description descriptionType="TechnicalInfo">
      # Archaeology demonstrates sustainable Ancestral Coast Salish salmon
      stewardship over thousands of years pre-contact: MALDI-TOF data --- Salmon
      are an essential component of the ecosystem in Tsleil-Waututh Nation’s
      traditional, ancestral, and unceded territory, centred on present-day
      Burrard Inlet, BC, Canada, where Tsleil-Waututh people have been
      harvesting salmon, along with a wide variety of other fishes, for
      millennia. Tsleil-Waututh Nation is an ancestral Coast Salish community
      that has called the Inlet home since time immemorial. This research
      assesses the continuity and sustainability of the salmon fishery at
      təmtəmíxʷtən, an ancestral Tsleil-Waututh settlement in the Inlet, over
      thousands of years before European contact (1792 CE). We apply
      Zooarchaeology by Mass Spectrometry (ZooMS) analysis to 245 archaeological
      salmon vertebrae to identify the species that were harvested by the
      Tsleil-Waututh community that lived at təmtəmíxʷtən. The results
      demonstrate that Tsleil-Waututh communities consistently and
      preferentially fished for chum salmon (*Oncorhynchus keta*) over the
      period of almost 3,000 years. The consistent abundance indicates a
      sustainable chum salmon fishery over that time and a strong
      salmon-to-people relationship through generations. This research supports
      Tsleil-Waututh Nation’s stewardship obligations under their ancestral
      legal principles to maintain conditions that uphold the Nation’s way of
      life. ## Description of the Data and file structure This zip file contains
      raw MALDI-TOF spectra for each of the 245 salmonid vertebrae sampled for
      ZooMS. Each sample was spotted in triplicate, along with calibration
      standards, onto a 384 spot Bruker MALDI ground steel target plate using 1
      µL of sample and 1 µL of α-cyano-hydroxycinnamic acid matrix. The samples
      were run on a Bruker ultraflex III MALDI TOF/TOF mass spectrometer with a
      Nd:YAG smart beam laser University of York in York, UK, and a SNAP
      averaging algorithm was used to obtain monoisotopic masses (C 4.9384, N
      1.3577, O 1.4773, S 0.0417, H 7.7583). Raw spectral data has been uploaded
      here for each sample in triplicate. Each sample is within an individual
      folder with the sample name (e.g., A713). Each raw spectrum is labelled
      with the sample name and the spot location on the MALDI Plate (e.g.,
      _A1,_A4, _A9). ## Sharing/access Information Links to other publicly
      accessible locations of the data: None.
    </description>
  </descriptions>
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