{"data":{"id":"10.5061/dryad.h9w0vt4t2","type":"dois","attributes":{"doi":"10.5061/dryad.h9w0vt4t2","prefix":"10.5061","suffix":"dryad.h9w0vt4t2","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"He, Guangfeng","nameType":"Personal","givenName":"Guangfeng","familyName":"He","affiliation":["Affiliated Hospital of Southwest Medical University"],"nameIdentifiers":[]},{"name":"Jiang, Lai","nameType":"Personal","givenName":"Lai","familyName":"Jiang","affiliation":["Southwest Medical University"],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0009-0005-4254-9338","nameIdentifierScheme":"ORCID"}]},{"name":"Zhou, Xuancheng","nameType":"Personal","givenName":"Xuancheng","familyName":"Zhou","affiliation":["Southwest Medical University"],"nameIdentifiers":[]},{"name":"Gu, Yuheng","nameType":"Personal","givenName":"Yuheng","familyName":"Gu","affiliation":["Southwest Medical University"],"nameIdentifiers":[]},{"name":"Tang, Jingyi","nameType":"Personal","givenName":"Jingyi","familyName":"Tang","affiliation":["Southwest Medical University"],"nameIdentifiers":[]},{"name":"Zhang, Qiang","nameType":"Personal","givenName":"Qiang","familyName":"Zhang","affiliation":["Southwest Medical University"],"nameIdentifiers":[]},{"name":"Hu, Qingwen","nameType":"Personal","givenName":"Qingwen","familyName":"Hu","affiliation":["Southwest Medical University"],"nameIdentifiers":[]},{"name":"Huang, Gang","nameType":"Personal","givenName":"Gang","familyName":"Huang","affiliation":["Southwest Medical University"],"nameIdentifiers":[]},{"name":"Zhuang, Ziye","nameType":"Personal","givenName":"Ziye","familyName":"Zhuang","affiliation":["Guangdong Medical College"],"nameIdentifiers":[]},{"name":"Gao, Xinrui","nameType":"Personal","givenName":"Xinrui","familyName":"Gao","affiliation":["Affiliated Hospital of Southwest Medical University"],"nameIdentifiers":[]},{"name":"Xu, Ke","nameType":"Personal","givenName":"Ke","familyName":"Xu","affiliation":["Chongqing General Hospital"],"nameIdentifiers":[]},{"name":"Xiao, Yewei","nameType":"Personal","givenName":"Yewei","familyName":"Xiao","affiliation":["Southwest Medical University"],"nameIdentifiers":[]}],"titles":[{"title":"RNA-seq of bone marrow samples from acute myeloid leukemia patients and healthy individuals"}],"publisher":"Dryad","container":{},"publicationYear":2024,"subjects":[{"subject":"FOS: Basic medicine","schemeUri":"https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf","subjectScheme":"fos"},{"subject":"FOS: Basic medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Acute myeloid leukemia","schemeUri":"https://github.com/PLOS/plos-thesaurus","subjectScheme":"PLOS Subject Area Thesaurus"},{"subject":"RNA-seq"},{"subject":"bone marrow fluid"}],"contributors":[],"dates":[{"date":"2024-11-23T14:19:44Z","dateType":"Created"},{"date":"2024-11-23T14:19:53Z","dateType":"Submitted"},{"date":"2024-12-10T00:00:00Z","dateType":"Issued"},{"date":"2024-12-10T00:00:00Z","dateType":"Available"}],"language":"en","types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsCitedBy","relatedIdentifier":"10.3389/fimmu.2024.1494106","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["16544361 bytes"],"formats":[],"version":"5","rightsList":[{"rights":"Creative Commons Zero v1.0 Universal","rightsUri":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc0-1.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Acute myeloid leukemia (AML) is the most common type of acute leukemia in\n adults, characterized by the malignant transformation and uncontrolled\n proliferation of abnormal myeloid hematopoietic progenitor cells in the\n bone marrow. This study aims to analyze the pathogenesis and prognosis of\n AML by performing RNA-seq on bone marrow samples from both AML patients\n and healthy individuals. We collected a total of 20 bone marrow samples,\n consisting of 10 samples from AML patients and 10 from healthy controls,\n all of which were stored in liquid nitrogen. The study received ethical\n approval (Ethical Approval Number: KY2024070), and all participants signed\n informed consent forms. This dataset includes the RNA-seq data\n from these samples. By analyzing the gene expression characteristics of\n myeloid progenitor cells, we attempt to construct a prognostic model to\n guide clinical treatment. These data not only aid in further exploring the\n molecular mechanisms of AML but also serve to identify new biomarkers and\n develop prognostic models, which are valuable for understanding the gene\n expression differences between AML and healthy bone marrow samples. The\n sharing of data from this study strictly adheres to ethical guidelines,\n with all sample collection and usage approved by the ethics committee and\n consented to by the participants.","descriptionType":"Abstract"},{"description":"A total of 20 bone marrow samples were collected for this study,\n consisting of 10 bone marrow samples from patients with acute myeloid\n leukemia (AML) (AML group), and 10 bone marrow samples from healthy\n individuals (control group). All AML patients were diagnosed by bone\n marrow smear morphology and cytogenetic testing, and individuals in the\n healthy control group underwent a thorough physical examination to exclude\n any history of blood disorders and tumors. The samples were stored in\n liquid nitrogen for further processing. All subjects signed an informed\n consent form, and the study was approved by the Ethics Committee of the\n Affiliated Hospital of Southwest Medical University (Ethics Approval No.\n KY2024070).\u003cbr\u003e\u003cbr\u003eTotal RNA was extracted from frozen bone\n marrow samples using TRIzol reagent (Thermo Fisher Scientific, USA) for\n RNA extraction according to the manufacturer's instructions. The\n quality of extracted RNA was assessed by Agilent 2100 Bioanalyzer to\n ensure that the RNA Integrity Index (RIN) was greater than 7.0.RNA\n concentration was quantified using Qubit 2.0 (Thermo Fisher Scientific) to\n ensure that sequencing requirements were met.RNA library construction was\n performed using the Illumina TruSeq RNA Library Prep Kit (Illumina, USA),\n and library quality control was performed by Qubit and Bioanalyzer. All\n samples were bipartite sequenced on the Illumina NovaSeq 6000 platform\n with a read length of 150 bp and a target sequencing depth of 50M reads\n per sample.","descriptionType":"Methods"},{"description":"# RNA-seq of bone marrow samples from acute myeloid leukemia patients and\n healthy individuals\n [https://doi.org/10.5061/dryad.h9w0vt4t2](https://doi.org/10.5061/dryad.h9w0vt4t2) ## Description of the data and file structure ### Files and variables #### File: AML.txt **Description:** RNA-seq expression matrix, rows are sample names, columns are gene names. ##### Variables * Gene_Name: gene name * Patient1: Bone marrow sample from patient 1 with acute myeloid leukemia * Patient2: Bone marrow sample from patient 2 with acute myeloid leukemia * Patient3: Bone marrow sample from patient 3 with acute myeloid leukemia * Patient4: Bone marrow sample from patient 4 with acute myeloid leukemia * Patient5: Bone marrow sample from patient 5 with acute myeloid leukemia * Patient6: Bone marrow sample from patient 6 with acute myeloid leukemia * Patient7: Bone marrow sample from patient 7 with acute myeloid leukemia * Patient8: Bone marrow sample from patient 8 with acute myeloid leukemia * Patient9: Bone marrow sample from patient 9 with acute myeloid leukemia * Patient10: Bone marrow sample from patient 10 with acute myeloid leukemia * Control1: Bone marrow sample from healthy person 1 * Control2: Bone marrow sample from healthy person 2 * Control3: Bone marrow sample from healthy person 3 * Control4: Bone marrow sample from healthy person 4 * Control5: Bone marrow sample from healthy person 5 * Control6: Bone marrow sample from healthy person 6 * Control7: Bone marrow sample from healthy person 7 * Control8: Bone marrow sample from healthy person 8 * Control9: Bone marrow sample from healthy person 9 * Control10: Bone marrow sample from healthy person 10 #### File: sample.txt **Description:** Sample Description Form ##### Variables * P: Bone marrow sample from a patient with acute myeloid leukemia * C: Bone marrow sample from a healthy person #### File: Raw\\_data.zip **Description:** In the Raw data section, we provide two TPM-formatted gene expression matrix files: \"gene.TPM.matrix.annot.xls\" includes the annotation of probes to gene names, while \"gene.tpm.matrix.xls\" contains the unannotated raw data. ##### Variables * gene_id: Unique identifiers for genes * gene_name: Name of the gene * description: Description or functional annotation of the gene * length: Length of the gene * Patient1 to Patient10: These columns represent the samples from 10 patients, with each column name followed by the corresponding patient number. These columns contain the expression levels of the gene in each patient sample, measured in TPM (Transcripts Per Million) units. * Control1 to Control10: These columns represent the samples from 10 normal individuals, with each column name followed by the corresponding normal individual number. They also contain the expression levels of the gene in each sample, in TPM units. * Control: The average expression level across all normal samples. * Patient: The average expression level across all patient samples. * nr: Likely refers to the identifier from the Non-Redundant Protein Sequence Database. * go: Gene Ontology terms describing the function, process, and location of the gene. * KO_id: KEGG Orthology ID used for classification in the KEGG database for enzymes and biological pathways. * KO_name: The name corresponding to the KEGG Orthology. * paths: The biological pathways in which the gene is involved. * cog: Likely an abbreviation for Cluster of Orthologous Groups, used for classification and functional annotation. * cog_description: The description of the cog. * pfam: Identifier from the Pfam database, a database of protein families. * uniprot: Protein identifier from the UniProt database, which provides protein sequence and functional information. * entrez: Entrez Gene ID, a gene identifier in the NCBI (National Center for Biotechnology Information) gene database.","descriptionType":"TechnicalInfo"}],"geoLocations":[],"fundingReferences":[],"xml":"<?xml version="1.0" encoding="UTF-8"?>
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    <creator>
      <creatorName nameType="Personal">He, Guangfeng</creatorName>
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      <affiliation affiliationIdentifier="https://ror.org/00g2rqs52" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Southwest Medical University</affiliation>
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      <creatorName nameType="Personal">Zhou, Xuancheng</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/00g2rqs52" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Southwest Medical University</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Gu, Yuheng</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/00g2rqs52" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Southwest Medical University</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Tang, Jingyi</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/00g2rqs52" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Southwest Medical University</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Zhang, Qiang</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/00g2rqs52" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Southwest Medical University</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Hu, Qingwen</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/00g2rqs52" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Southwest Medical University</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Huang, Gang</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/00g2rqs52" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Southwest Medical University</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Zhuang, Ziye</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/04k5rxe29" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Guangdong Medical College</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Gao, Xinrui</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/0014a0n68" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Affiliated Hospital of Southwest Medical University</affiliation>
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    <creator>
      <creatorName nameType="Personal">Xu, Ke</creatorName>
      <affiliation>Chongqing General Hospital</affiliation>
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    <creator>
      <creatorName nameType="Personal">Xiao, Yewei</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/00g2rqs52" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Southwest Medical University</affiliation>
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  <titles>
    <title>
      RNA-seq of bone marrow samples from acute myeloid leukemia patients and
      healthy individuals
    </title>
  </titles>
  <publisher publisherIdentifier="https://ror.org/00x6h5n95" publisherIdentifierScheme="ROR" schemeURI="https://ror.org/">Dryad</publisher>
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    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Acute myeloid leukemia</subject>
    <subject>RNA-seq</subject>
    <subject>bone marrow fluid</subject>
  </subjects>
  <dates>
    <date dateType="Created">2024-11-23T14:19:44Z</date>
    <date dateType="Submitted">2024-11-23T14:19:53Z</date>
    <date dateType="Issued">2024-12-10T00:00:00Z</date>
    <date dateType="Available">2024-12-10T00:00:00Z</date>
  </dates>
  <language>en</language>
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    <description descriptionType="Abstract">
      Acute myeloid leukemia (AML) is the most common type of acute leukemia in
      adults, characterized by the malignant transformation and uncontrolled
      proliferation of abnormal myeloid hematopoietic progenitor cells in the
      bone marrow. This study aims to analyze the pathogenesis and prognosis of
      AML by performing RNA-seq on bone marrow samples from both AML patients
      and healthy individuals. We collected a total of 20 bone marrow samples,
      consisting of 10 samples from AML patients and 10 from healthy controls,
      all of which were stored in liquid nitrogen. The study received ethical
      approval (Ethical Approval Number: KY2024070), and all participants signed
      informed consent forms.&amp;nbsp;This dataset includes the RNA-seq data
      from these samples. By analyzing the gene expression characteristics of
      myeloid progenitor cells, we attempt to construct a prognostic model to
      guide clinical treatment. These data not only aid in further exploring the
      molecular mechanisms of AML but also serve to identify new biomarkers and
      develop prognostic models, which are valuable for understanding the gene
      expression differences between AML and healthy bone marrow samples. The
      sharing of data from this study strictly adheres to ethical guidelines,
      with all sample collection and usage approved by the ethics committee and
      consented to by the participants.
    </description>
    <description descriptionType="Methods">
      &lt;p&gt;A total of 20 bone marrow samples were collected for this study,
      consisting of 10 bone marrow samples from patients with acute myeloid
      leukemia (AML) (AML group), and 10 bone marrow samples from healthy
      individuals (control group). All AML patients were diagnosed by bone
      marrow smear morphology and cytogenetic testing, and individuals in the
      healthy control group underwent a thorough physical examination to exclude
      any history of blood disorders and tumors. The samples were stored in
      liquid nitrogen for further processing. All subjects signed an informed
      consent form, and the study was approved by the Ethics Committee of the
      Affiliated Hospital of Southwest Medical University (Ethics Approval No.
      KY2024070).&lt;br&gt;&lt;br&gt;Total RNA was extracted from frozen bone
      marrow samples using TRIzol reagent (Thermo Fisher Scientific, USA) for
      RNA extraction according to the manufacturer's instructions. The
      quality of extracted RNA was assessed by Agilent 2100 Bioanalyzer to
      ensure that the RNA Integrity Index (RIN) was greater than 7.0.RNA
      concentration was quantified using Qubit 2.0 (Thermo Fisher Scientific) to
      ensure that sequencing requirements were met.RNA library construction was
      performed using the Illumina TruSeq RNA Library Prep Kit (Illumina, USA),
      and library quality control was performed by Qubit and Bioanalyzer. All
      samples were bipartite sequenced on the Illumina NovaSeq 6000 platform
      with a read length of 150 bp and a target sequencing depth of 50M reads
      per sample.&lt;/p&gt;
    </description>
    <description descriptionType="TechnicalInfo">
      # RNA-seq of bone marrow samples from acute myeloid leukemia patients and
      healthy individuals
      [https://doi.org/10.5061/dryad.h9w0vt4t2](https://doi.org/10.5061/dryad.h9w0vt4t2) ## Description of the data and file structure ### Files and variables #### File: AML.txt **Description:** RNA-seq expression matrix, rows are sample names, columns are gene names. ##### Variables * Gene_Name: gene name * Patient1: Bone marrow sample from patient 1 with acute myeloid leukemia * Patient2: Bone marrow sample from patient 2 with acute myeloid leukemia * Patient3: Bone marrow sample from patient 3 with acute myeloid leukemia * Patient4: Bone marrow sample from patient 4 with acute myeloid leukemia * Patient5: Bone marrow sample from patient 5 with acute myeloid leukemia * Patient6: Bone marrow sample from patient 6 with acute myeloid leukemia * Patient7: Bone marrow sample from patient 7 with acute myeloid leukemia * Patient8: Bone marrow sample from patient 8 with acute myeloid leukemia * Patient9: Bone marrow sample from patient 9 with acute myeloid leukemia * Patient10: Bone marrow sample from patient 10 with acute myeloid leukemia * Control1: Bone marrow sample from healthy person 1 * Control2: Bone marrow sample from healthy person 2 * Control3: Bone marrow sample from healthy person 3 * Control4: Bone marrow sample from healthy person 4 * Control5: Bone marrow sample from healthy person 5 * Control6: Bone marrow sample from healthy person 6 * Control7: Bone marrow sample from healthy person 7 * Control8: Bone marrow sample from healthy person 8 * Control9: Bone marrow sample from healthy person 9 * Control10: Bone marrow sample from healthy person 10 #### File: sample.txt **Description:** Sample Description Form ##### Variables * P: Bone marrow sample from a patient with acute myeloid leukemia * C: Bone marrow sample from a healthy person #### File: Raw\_data.zip **Description:** In the Raw data section, we provide two TPM-formatted gene expression matrix files: "gene.TPM.matrix.annot.xls" includes the annotation of probes to gene names, while "gene.tpm.matrix.xls" contains the unannotated raw data. ##### Variables * gene_id: Unique identifiers for genes * gene_name: Name of the gene * description: Description or functional annotation of the gene * length: Length of the gene * Patient1 to Patient10: These columns represent the samples from 10 patients, with each column name followed by the corresponding patient number. These columns contain the expression levels of the gene in each patient sample, measured in TPM (Transcripts Per Million) units. * Control1 to Control10: These columns represent the samples from 10 normal individuals, with each column name followed by the corresponding normal individual number. They also contain the expression levels of the gene in each sample, in TPM units. * Control: The average expression level across all normal samples. * Patient: The average expression level across all patient samples. * nr: Likely refers to the identifier from the Non-Redundant Protein Sequence Database. * go: Gene Ontology terms describing the function, process, and location of the gene. * KO_id: KEGG Orthology ID used for classification in the KEGG database for enzymes and biological pathways. * KO_name: The name corresponding to the KEGG Orthology. * paths: The biological pathways in which the gene is involved. * cog: Likely an abbreviation for Cluster of Orthologous Groups, used for classification and functional annotation. * cog_description: The description of the cog. * pfam: Identifier from the Pfam database, a database of protein families. * uniprot: Protein identifier from the UniProt database, which provides protein sequence and functional information. * entrez: Entrez Gene ID, a gene identifier in the NCBI (National Center for Biotechnology Information) gene database.
    </description>
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