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Retrogenic mice were immunized with\n OVA mRNA-LNP or OVA protein + poly I/C, and SIINFEKL/Kb tetramer-positive\n CD8 T cells were analyzed on day 14. TCR sequencing analysis showed that\n the number of TCR clonotypes was markedly reduced in SIINFEKL/Kb\n tetramer-positive CD8 T cells compared to total CD8 T cells before\n immunization. Principal component analysis revealed a marked similarity in\n the composition of OVA peptide-reactive TCRs between mice immunized with\n OVA mRNA-LNP and those receiving OVA protein + poly I/C. These results\n showed similar TCR repertoires induced by OVA mRNA-LNP and OVA + poly I/C,\n suggesting that the robust expansion of CD8 T cells by mRNA-LNP is\n primarily due to differences that are quantitative rather than qualitative\n compared to antigen protein and conventional adjuvants. The sequencing\n data have been deposited in the DDBJ Sequence Read Archive under\n BioProject accession number PRJDB40453.","descriptionType":"Abstract"},{"description":"# Code from: A distinct antigen presentation pathway drive potent T-cell\n immunity in lipid nanoparticle-based mRNA vaccines Dataset DOI:\n [10.5061/dryad.h44j0zq25](https://doi.org/10.5061/dryad.h44j0zq25) ##\n Description of the data and file structure The source code for the\n principal component analysis of TCR frequency, shown in Figure S5B (Muro\n et al, Science Advances 2026). ### Files and variables #### File: R_script\n **Description:** The source code for the principal component analysis of\n TCR frequency, shown in Figure S5B ## Code/software This is an R script\n for performing principal component analysis on the frequencies of\n TCR-alpha clones obtained from bulk TCR sequence analysis. ## Access\n information Other publicly accessible locations of the data: * GitHub\n ([https://github.com/nittatakeshi](https://github.com/nittatakeshi)) Data\n was derived from the following sources: * The raw TCR sequencing data have\n been deposited in the DDBJ Sequence Read Archive under BioProject\n accession number PRJDB40453.","descriptionType":"TechnicalInfo"}],"geoLocations":[],"fundingReferences":[{"funderName":"Japan Agency for Medical Research and Development","funderIdentifier":"https://ror.org/004rtk039","funderIdentifierType":"ROR"},{"funderName":"Japan Society for the Promotion of Science","funderIdentifier":"https://ror.org/00hhkn466","funderIdentifierType":"ROR"},{"funderName":"Secom (Japan)","funderIdentifier":"https://ror.org/05ggzej07","funderIdentifierType":"ROR"},{"funderName":"Takeda Science Foundation","funderIdentifier":"https://ror.org/02y123g31","funderIdentifierType":"ROR"},{"funderName":"Astellas Pharma (Japan)","funderIdentifier":"https://ror.org/01cjash87","funderIdentifierType":"ROR"},{"funderName":"Uehara Memorial Foundation","funderIdentifier":"https://ror.org/00gc20a07","funderIdentifierType":"ROR"},{"funderName":"Nakatomi Foundation","funderIdentifier":"https://ror.org/0196vdc40","funderIdentifierType":"ROR"},{"funderName":"Tokyo University of Science","funderIdentifier":"https://ror.org/05sj3n476","funderIdentifierType":"ROR"},{"funderName":"Kanazawa University","funderIdentifier":"https://ror.org/02hwp6a56","funderIdentifierType":"ROR"},{"funderName":"Novartis (Japan)","funderIdentifier":"https://ror.org/01k1ftz35","funderIdentifierType":"ROR"},{"funderName":"Hamaguchi Foundation for the Advancement of Biochemistry","funderIdentifier":"https://ror.org/01t80ed43","funderIdentifierType":"ROR"},{"funderName":"Kowa Life Science Foundation","funderIdentifier":"https://ror.org/04jpf9990","funderIdentifierType":"ROR"}],"xml":"<?xml version="1.0" encoding="UTF-8"?>
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      Code from: A distinct antigen presentation pathway drive potent T-cell
      immunity in lipid nanoparticle-based mRNA vaccines
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      In the paper by Muro et al (Science Advances 2026), to investigate whether
      mRNA-LNP affects the TCR repertoire of the induced CD8 T cells, we
      performed bulk TCR sequencing on the TCRα chains in retrogenic mice
      expressing a rearranged TCRβ chain. Retrogenic mice were immunized with
      OVA mRNA-LNP or OVA protein + poly I/C, and SIINFEKL/Kb tetramer-positive
      CD8 T cells were analyzed on day 14. TCR sequencing analysis showed that
      the number of TCR clonotypes was markedly reduced in SIINFEKL/Kb
      tetramer-positive CD8 T cells compared to total CD8 T cells before
      immunization. Principal component analysis revealed a marked similarity in
      the composition of OVA peptide-reactive TCRs between mice immunized with
      OVA mRNA-LNP and those receiving OVA protein + poly I/C. These results
      showed similar TCR repertoires induced by OVA mRNA-LNP and OVA + poly I/C,
      suggesting that the robust expansion of CD8 T cells by mRNA-LNP is
      primarily due to differences that are quantitative rather than qualitative
      compared to antigen protein and conventional adjuvants. The sequencing
      data have been deposited in the DDBJ Sequence Read Archive under
      BioProject accession number PRJDB40453.
    </description>
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      # Code from: A distinct antigen presentation pathway drive potent T-cell
      immunity in lipid nanoparticle-based mRNA vaccines Dataset DOI:
      [10.5061/dryad.h44j0zq25](https://doi.org/10.5061/dryad.h44j0zq25) ##
      Description of the data and file structure The source code for the
      principal component analysis of TCR frequency, shown in Figure S5B (Muro
      et al, Science Advances 2026). ### Files and variables #### File: R_script
      **Description:** The source code for the principal component analysis of
      TCR frequency, shown in Figure S5B ## Code/software This is an R script
      for performing principal component analysis on the frequencies of
      TCR-alpha clones obtained from bulk TCR sequence analysis. ## Access
      information Other publicly accessible locations of the data: * GitHub
      ([https://github.com/nittatakeshi](https://github.com/nittatakeshi)) Data
      was derived from the following sources: * The raw TCR sequencing data have
      been deposited in the DDBJ Sequence Read Archive under BioProject
      accession number PRJDB40453.
    </description>
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