{"data":{"id":"10.5061/dryad.fqz612jvx","type":"dois","attributes":{"doi":"10.5061/dryad.fqz612jvx","prefix":"10.5061","suffix":"dryad.fqz612jvx","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Yang, Xinwang","nameType":"Personal","givenName":"Xinwang","familyName":"Yang","affiliation":["Kunming Medical University"],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0000-0003-3210-8908","nameIdentifierScheme":"ORCID"}]}],"titles":[{"title":"Data from: A short peptide exerts neuroprotective effects on cerebral ischemia-reperfusion injury by reducing inflammation via the miR-6328/IKKβ/NF-κB axis"}],"publisher":"Dryad","container":{},"publicationYear":2022,"subjects":[{"subject":"FOS: Basic medicine","schemeUri":"https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf","subjectScheme":"fos"},{"subject":"FOS: Basic medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2022-08-17T15:54:05Z","dateType":"Submitted"},{"date":"2022-08-28T00:00:00Z","dateType":"Issued"},{"date":"2022-08-28T00:00:00Z","dateType":"Available"}],"language":"en","types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsCitedBy","relatedIdentifier":"10.1186/s12974-023-02739-4","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["8881373 bytes"],"formats":[],"version":"4","rightsList":[{"rights":"Creative Commons Zero v1.0 Universal","rightsUri":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc0-1.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Background Despite considerable efforts, ischemic stroke (IS) remains a\n challenging clinical problem. Therefore, the discovery of effective\n therapeutic and targeted drugs based on the underlying molecular mechanism\n is crucial for effective IS treatment. Methods A cDNA-encoding peptide was\n extracted and identified from RNA extracted from Rana limnocharis skin,\n and its neuroprotective properties were evaluated using a middle cerebral\n artery occlusion/reperfusion (MCAO/R) model in rats and an oxygen-glucose\n deprivation/reperfusion (OGD/R) model in neuron-like PC12 cells. The\n underlying molecular mechanisms were explored using microRNA (miRNA)\n sequencing, quantitative real-time polymerase chain reaction,\n dual-luciferase reporter gene assay, and western blotting. Results A new\n peptide (NP1) with an amino acid sequence of ‘FLPAAICLVIKTC’ was\n identified. NP1 showed no obvious toxicities in vivo and in vitro.\n Intraperitoneal administration of NP1 (10 nmol/kg) effectively reduced the\n volume of cerebral infarction and relieved neurological dysfunction in\n MCAO/R model rats. Moreover, NP1 significantly alleviated the decrease in\n viability of PC12 cells induced by OGD/R. NP1 effectively suppressed\n inflammation by reducing interleukin-1β (IL-1β) and tumor necrosis\n factor-α (TNF-α) levels in vitro and in vivo. Furthermore, NP1\n up-regulated the expression of miRNA-6328, which targeted down-regulation\n of the inflammation-related factor inhibitor kappa B kinase β (IKKβ),\n thereby reducing nuclear factor-kappa B p65 (NF-κB p65) and inhibitor of\n NF-κB (I-κB) phosphorylation and inhibiting the NF-κB pathway. Conclusions\n NP1 exerted neuroprotective effects by reducing inflammation via the\n miRNA-6328/IKKβ/NF-κB axis. This study highlights the importance of\n peptides in the development of new drugs, elucidation of pathological\n mechanisms, and discovery of new drug targets.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[{"schemeUri":"https://ror.org","funderName":"National Natural Science Foundation of China","funderIdentifier":"https://ror.org/01h0zpd94","funderIdentifierType":"ROR"}],"xml":"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