{"data":{"id":"10.5061/dryad.dr7sqvbb3","type":"dois","attributes":{"doi":"10.5061/dryad.dr7sqvbb3","prefix":"10.5061","suffix":"dryad.dr7sqvbb3","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Xu, Guilian","nameType":"Personal","givenName":"Guilian","familyName":"Xu","affiliation":["University of Florida"],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0000-0001-9630-1534","nameIdentifierScheme":"ORCID"}]},{"name":"Lopez, Amanda","nameType":"Personal","givenName":"Amanda","familyName":"Lopez","affiliation":["University of Florida"],"nameIdentifiers":[]},{"name":"Brkic, Selma","nameType":"Personal","givenName":"Selma","familyName":"Brkic","affiliation":["University of Florida"],"nameIdentifiers":[]},{"name":"Fromholt, Susan","nameType":"Personal","givenName":"Susan","familyName":"Fromholt","affiliation":["University of Florida"],"nameIdentifiers":[]},{"name":"Chakrabarty, Paramita","nameType":"Personal","givenName":"Paramita","familyName":"Chakrabarty","affiliation":["University of Florida"],"nameIdentifiers":[]},{"name":"Borchelt, David","nameType":"Personal","givenName":"David","familyName":"Borchelt","affiliation":["University of Florida"],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0000-0002-0813-6979","nameIdentifierScheme":"ORCID"}]}],"titles":[{"title":"Data from: Efficient induction of motor neuron disease in transgenic G93A SOD1 mice by prion-like seeding"}],"publisher":"Dryad","container":{},"publicationYear":2026,"subjects":[{"subject":"FOS: Biological sciences","schemeUri":"https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf","subjectScheme":"fos"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Basic medicine","schemeUri":"https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf","subjectScheme":"fos"},{"subject":"FOS: Basic medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Amyotrophic lateral sclerosis","schemeUri":"https://github.com/PLOS/plos-thesaurus","subjectScheme":"PLOS Subject Area Thesaurus"},{"subject":"Superoxide dismutase-1"},{"subject":"Prions","schemeUri":"https://github.com/PLOS/plos-thesaurus","subjectScheme":"PLOS Subject Area Thesaurus"},{"subject":"strains"}],"contributors":[{"name":"University of Florida","affiliation":[],"contributorType":"Sponsor","nameIdentifiers":[]}],"dates":[{"date":"2026-02-23T21:21:08Z","dateType":"Created"},{"date":"2026-02-23T21:37:45Z","dateType":"Submitted"},{"date":"2026-04-15T00:00:00Z","dateType":"Issued"},{"date":"2026-04-15T00:00:00Z","dateType":"Available"}],"language":"en","types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsCitedBy","relatedIdentifier":"10.1080/19336896.2026.2630484","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["84440160086 bytes"],"formats":[],"version":"5","rightsList":[{"rights":"Creative Commons Zero v1.0 Universal","rightsUri":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc0-1.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Mutations in superoxide dismutase 1 (SOD1) cause paralysis in familial\n amyotrophic lateral sclerosis and promote its misfolding into neurotoxic\n aggregates. Previous studies have shown that mice expressing the\n ALS-causing G85R variant of SOD1 develop paralysis much faster after\n intraspinal injection of spinal homogenates from paralyzed G85R SOD1 mice.\n These findings, and other studies in cell models, established the prionoid\n templating properties of misfolded mutant SOD1. Previously, however, we\n noted that the widely used Gur1-G93A SOD1 mice, which express at high\n levels and develop paralysis by 6 months of age, were resistant to seeding\n by homogenates from paralyzed G93A mice. A line of G93A mice that express\n at very low levels (VLE-G93A) were responsive to seeding but at low\n efficiency. The poor susceptibility of G93A-SOD1 mice to seeding was not\n what we expected if prion-like propagation is essential to SOD1 ALS\n pathogenesis. In our prior, studies seeding homogenates from paralyzed\n G93A-SOD1 mice were injected into the spine of newborn mice, leading us to\n question whether older G93A SOD1 mice might be more susceptible to\n seeding. Here, we establish that adult VLE G93A SOD1 mice (up to 12 months\n of age) injected intrathecally with seeding homogenates containing\n misfolded G93A or G85R SOD1 developed accelerated motor neuron disease\n efficiently. Thus, we demonstrate that both the route and age of\n inoculation can influence the efficiency of SOD1 seeding to induce motor\n neuron disease in VLE G93A-SOD1 mice. These data, together with our\n earlier reports, suggest that prion-like templating contributes to disease\n progression in SOD1-ALS.","descriptionType":"Abstract"},{"description":"\u003cem\u003e\u003cstrong\u003eStudy\n Design\u003c/strong\u003e\u003c/em\u003e The primary goal of\n the study was to determine whether older VLE G93A-SOD1 mice may be more\n susceptible to prion-like seeding by intrathecal administration of seeding\n homogenates. We injected VLE G93A mice with homogenates from paralyzed\n G93A SOD1 mice or pooled homogenates from paralyzed mice expressing\n G85R-SOD1 fused to yellow fluorescent protein (YFP) that had been made\n paralyzed by injection of homogenates from paralyzed G93A SOD1 mice\n (designated P2-G93A seeding pool). Initially, we challenged 12-month-old\n mice with both seeding homogenates and then subsequently determined\n whether younger VLE G93A-SOD1 mice were also susceptible, using the\n P2-G93A pool. Controls for these experiments included uninjected VLE\n G93A-SOD1 mice and mice injected with a pooled homogenate from young\n asymptomatic naïve 2-month-old Line 230 mice (Y230 pool). Pathological\n analyses of these animals produced image data for Figures 2 - 4 of the\n accompanying publication. In prelude to the G93A mouse\n study, we first sought to compare the relative seeding efficacy of\n different injection routes using a highly permissive line of homozygous\n mice expressing G85R-SOD1:YFP (Line 230 model). The seeding-homogenates\n for these studies were either the control  Y230-pool or a pool of\n homogenates from seeded paralyzed G85R-SOD1:YFP mice. We compared the\n incubation time to paralysis following lumbar intraspinal (ISP) injection\n in P0 newborn mice to lumbar intrathecal injection in 2 month old Line 230\n mice. Pathological analyses of these animals produced image data for\n Figure 1 of the accompanying publication.            \n For most of these injections, we sought to inject mice from 2 to 3\n litters. The homozygous 230 mice naturally produced larger cohorts. In the\n VLE G93A study, each cohort receiving the G93A or P2-G93A pool was between\n 8 and 10 transgene positive animals. Only one litter of VLE G93A mice was\n injected with the 230 pool and Y230 pool inoculum, producing smaller\n cohorts of transgene positive animals.             The\n primary humane endpoint for all injections was overt paralysis of at least\n one hindlimb. In a subset of mice, other body condition factors such as\n hydration or muscle atrophy were considered in decisions to euthanize and\n harvest tissues from mice that were obviously weak but not yet paralyzed.\n Laboratory staff were not blind to treatment or genotype, but veterinary\n staff that assisted in determining endpoint were blind. Mice that were\n recommended for euthanasia due to fighting wounds or tumor development\n were excluded from the study. No mice that received seeding injections for\n data in Figure 1 were excluded from the study. For the data in Figure 2,\n there were mice that had to be excluded post-injection; Group 1 – 2 mice\n and Group 3 – 1 mouse.               Mice that were\n identified as distressed and recommended for euthanasia but did not\n otherwise exhibit signs of paralysis were excluded from statistical\n analyses of incubation periods. For data in Figure 2B there were the\n following exclusions: Group 1 – 1 animal, Group 2 – 2 animals, Group 3 – 1\n animal, Group 5 – 2 animals, Group 7 – 3 animals.   \n \u003cem\u003e\u003cstrong\u003eNeuropathology\u003c/strong\u003e\u003c/em\u003e Detailed methods for tissue collection and neuropathology are described in Ayers et al \u003csup\u003e23\u003c/sup\u003e. For imaging YFP fluorescence, paraffin-embedded tissues were cut at 5 μm thickness and then deparaffinized and mounted on coverslips with water; images were visualized by epifluorescence Olympus BX60 microscope. After imaging, the coverslips were removed, and the slides were stained by Campbell-Switzer silver stain as previously described \u003csup\u003e23,34\u003c/sup\u003e. Ubiquitin staining was performed as described previously \u003csup\u003e39\u003c/sup\u003e. Paraffin sections were deparaffinized, steamed in citrate buffer (10 mM citrate, pH 6.0, with 0.05% Tween-20) for 20 minutes. After rinsing the slides with tap water, the slides were blocked with 3% normal goat serum in PBS containing Tween 20, and then incubated overnight with ubiquitin antibody (mouse monoclonal MCA-Ubi-1, 1:1000; Encor Biotechnology, Gainesville, FL). After quenching with 0.03% hydrogen peroxide in PBS-T, sections were treated with a biotinylated anti-mouse secondary antibody (1:500; Vector Laboratories, Newark, CA), followed by ABC-HRP and DAB (KPL, Seracare, MD) detection. Slides were counterstained with hematoxylin. Images were acquired at 20× using an Aperio ScanScope XT (Leica Biosystems), processed in ImageScope, and edited in GIMP ((GNU Image Manipulation Program v3.0.4: gimp.org) for cropping and scale bar addition based on pixel calibration.","descriptionType":"Methods"},{"description":"# Data from: Efficient induction of motor neuron disease in transgenic\n G93A SOD1 mice by prion-like seeding This repository contains animal\n metadata and histological image data used in the study.\\ All files are\n organized to ensure **clear traceability between animals, experimental\n conditions referenced in the manuscript, and figure panels**, while\n supporting **efficient data access, sharing, and repository submission**.\n --- ## Animal Metadata The image files are documentation of pathologic\n features in mice that develop paralysis as a result of the injection of\n tissue homogenates from paralyzed mice. All animals used in this study are\n documented an Excel file: * **Xu_et_al_mouse_list.xlsx** This Excel file\n contains **11 tabs**, each corresponding to animals contributing to a\n specific figure in the manuscript. The Tab names refer to the animals used\n in generating each figure in publication and the tissue homogenate used to\n induce early paralysis, listed below. The animals used in Figures 3 and 4\n are the same as those used in Figure 2; tissue sections have been stained\n by different methods. **Figure 1A-D (YFP), Figure 1B’-D’ (silver\n staining): Tab names** ·                     Fig1 P0 ISP Y230\n ·                     Fig1 P0 ISP 230 pool ·                     Fig 1\n Int-230 pool @2mo --- **Figure 2, Figure 3 (silver staining), Figure 4\n (Ubi-1 staining)**: **Tab names** ·                     Fig 2A Uninjected\n ·                     Fig 2B G93A 12mo ·                     Fig 2B\n P2-G93A 12mo ·                     Fig 2B P2-G93A P0 ·                    \n Fig 2B P2-G93A 2mo ·                     Fig 2B P2-G93A 6mo\n ·                     Fig 2B 230 pool 12mo ·                     Fig 2B\n Y230 12mo **Description of column names within each tab.** MouseID - Each\n animal receives a **mouse cage ID** (assigned by the University of Florida\n Animal Care Services) and **tail tattoo number** (1-5 per cage). Together\n these numbers create a mouse identification number (). Sex of the animal\n is indicated. Age indicates the cumulative age at the time of euthanasia.\n Symptoms - notes on clinical evaluation at time of euthanasia Incubation-\n notes the the incubation period (interval between injection of homogenates\n and appearance of paralysis) in months (mo). The relative severity of\n pathologic abnormalities in spinal cord is noted by +, ++, or +++ rating\n for each of the following.  YFP aggregates refers to direct fluorescence\n of pathologic inclusions produced by mutant superoxide dismutase 1 fused\n to yellow fluorescent protein (YFP). Silver staining refers to tissues\n stained by the Campbell-Switzer method of silver staining to secondarily\n confirm pathology.  Ubi-1 refers tissues stained by antibodies to\n ubiquitin to reveal pathology in mice that express mutant superoxide\n dismutase 1 lacking the YFP tag.  n.d. indicates not done Notes -\n indicates which images were used in specific Figures in the accompanying\n publication. \n \n --- ## File Overview --- ## Image Data Packaging Strategy All image data\n related to this study are stored in four main folders mainly according to\n the figure number. Each folder is further subdivided according to the\n experimental groups referenced in the figure panels. The data were\n compressed into four primary ZIP archives. Each ZIP archive corresponds to\n a figure (or group of figures) in the manuscript and contains subfolders\n organized by figure panel, experimental group, and time point. ·    **YFP\n images** were captured using a **fluorescence microscope** and are saved\n in **.tiff** format. ·    **Silver-stained** and **Ubi-1 immunostained**\n slides were scanned using the **Aperio digital slide scanning system**.\n These images are saved in **.svs** format and can be viewed using\n **ScanScope** (free software\n [https://www.leicabiosystems.com/us/digital-pathology/manage/aperio-imagescope/](https://www.leicabiosystems.com/us/digital-pathology/manage/aperio-imagescope/)). --- ## Zipped Image Archives * **Figure_1A-D_(YFP).zip**\\ YFP fluorescence microscopy images corresponding to Figure 1A–D. * **Figure_1B’-D’_(silver_staining).zip**\\ Silver-stained images corresponding to Figure 1B′–D′. * **Figure_2_and_3_(silver_staining).zip**\\ Silver-stained images corresponding to Figures 2 and 3. * **Figure_4_(UBi-1_staining).zip**\\ Ubi-1 immunostained images corresponding to Figure 4. --- ## Directory Structure Inside ZIP Archives Each ZIP archive preserves a consistent internal directory structure.\\ The folders listed below represent **subdirectories contained within the corresponding ZIP files** and map directly to figure panels in the manuscript. ### Figure 1A–D (YFP) * `Fig 1 Int-230 pool @2mo` * `Fig1 P0 ISP 230 pool` * `Fig1 P0 ISP Y230 control` --- ### Figure 1B′–D′ (Silver Staining) * `Fig 1 Int-230 pool @2mo silver` * `Fig 1 P0 ISP 230 pool silver` * `Fig 1 P0 ISP Y230 control silver` --- ### Figures 2 and 3 (Silver Staining) * `Figure (2A, 3A) G93A Uninjected` * `Figure (2B-1, 3D) G93A G93A@12mo` * `Figure (2B-2, 3E) P2-G93A pool@12mo` * `Figure (2B-3, 3G) P2-G93A pool@0mo` * `Figure (2B-4, 3H) P2-G93A pool@2mo` * `Figure (2B-5, 3I) P2-G93A pool@6mo` * `Figure (2B-6, 3C) G93A 230 pool@12mo` * `Figure (2B-7, 3B) G93A Y230 pool@12mo` * `Figure (3F) G93A G93A@0mo` --- ### Figure 4 (Ubi-1 Staining) * `Figure 4A G93A Uninjected (Ubi-1)` * `Figure 4B G93A Y230 pool@12mo (Ubi-1)` * `Figure 4C G93A 230 pool@12mo (Ubi-1)` * `Figure 4D G93A G93A@12mo (Ubi-1)` * `Figure 4E P2-G93A pool@12mo (Ubi-1)` * `Figure 4F G93A G93A@0mo (Ubi-1)` * `Figure 4G P2-G93A pool@0mo (Ubi-1)` * `Figure 4H P2-G93A pool@2mo (Ubi-1)` * `Figure 4I P2-G93A pool@6mo (Ubi-1)`","descriptionType":"TechnicalInfo"}],"geoLocations":[],"fundingReferences":[{"schemeUri":"https://ror.org","awardTitle":"Modeling the progression of SOD1-linked motor neuron disease","funderName":"National Institute of Neurological Disorders and Stroke","awardNumber":"2R01NS092788-06","funderIdentifier":"https://ror.org/01s5ya894","funderIdentifierType":"ROR"}],"xml":"<?xml version="1.0" encoding="UTF-8"?>
<resource xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns="http://datacite.org/schema/kernel-4" xsi:schemaLocation="http://datacite.org/schema/kernel-4 http://schema.datacite.org/meta/kernel-4/metadata.xsd">
  <identifier identifierType="DOI">10.5061/DRYAD.DR7SQVBB3</identifier>
  <creators>
    <creator>
      <creatorName nameType="Personal">Xu, Guilian</creatorName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0001-9630-1534</nameIdentifier>
      <affiliation affiliationIdentifier="https://ror.org/02y3ad647" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of Florida</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Lopez, Amanda</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/02y3ad647" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of Florida</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Brkic, Selma</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/02y3ad647" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of Florida</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Fromholt, Susan</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/02y3ad647" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of Florida</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Chakrabarty, Paramita</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/02y3ad647" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of Florida</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Borchelt, David</creatorName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0002-0813-6979</nameIdentifier>
      <affiliation affiliationIdentifier="https://ror.org/02y3ad647" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of Florida</affiliation>
    </creator>
  </creators>
  <titles>
    <title>
      Data from: Efficient induction of motor neuron disease in transgenic G93A
      SOD1 mice by prion-like seeding
    </title>
  </titles>
  <publisher publisherIdentifier="https://ror.org/00x6h5n95" publisherIdentifierScheme="ROR" schemeURI="https://ror.org/">Dryad</publisher>
  <resourceType resourceTypeGeneral="Dataset">dataset</resourceType>
  <publicationYear>2026</publicationYear>
  <subjects>
    <subject subjectScheme="fos" schemeURI="https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf">FOS: Biological sciences</subject>
    <subject subjectScheme="fos" schemeURI="https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf">FOS: Basic medicine</subject>
    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Amyotrophic lateral sclerosis</subject>
    <subject>Superoxide dismutase-1</subject>
    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Prions</subject>
    <subject>strains</subject>
  </subjects>
  <fundingReferences>
    <fundingReference>
      <funderName>National Institute of Neurological Disorders and Stroke</funderName>
      <funderIdentifier funderIdentifierType="ROR">https://ror.org/01s5ya894</funderIdentifier>
      <awardNumber>2R01NS092788-06</awardNumber>
      <awardTitle>Modeling the progression of SOD1-linked motor neuron disease</awardTitle>
    </fundingReference>
  </fundingReferences>
  <contributors>
    <contributor contributorType="Sponsor">
      <contributorName>University of Florida</contributorName>
    </contributor>
  </contributors>
  <dates>
    <date dateType="Created">2026-02-23T21:21:08Z</date>
    <date dateType="Submitted">2026-02-23T21:37:45Z</date>
    <date dateType="Issued">2026-04-15T00:00:00Z</date>
    <date dateType="Available">2026-04-15T00:00:00Z</date>
  </dates>
  <language>en</language>
  <relatedIdentifiers>
    <relatedIdentifier relationType="IsCitedBy" relatedIdentifierType="DOI">https://doi.org/10.1080/19336896.2026.2630484</relatedIdentifier>
  </relatedIdentifiers>
  <sizes>
    <size>84440160086 bytes</size>
  </sizes>
  <version>5</version>
  <rightsList>
    <rights rightsURI="https://spdx.org/licenses/CC0-1.0.html">Creative Commons Zero v1.0 Universal</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">
      Mutations in superoxide dismutase 1 (SOD1) cause paralysis in familial
      amyotrophic lateral sclerosis and promote its misfolding into neurotoxic
      aggregates. Previous studies have shown that mice expressing the
      ALS-causing G85R variant of SOD1 develop paralysis much faster after
      intraspinal injection of spinal homogenates from paralyzed G85R SOD1 mice.
      These findings, and other studies in cell models, established the prionoid
      templating properties of misfolded mutant SOD1. Previously, however, we
      noted that the widely used Gur1-G93A SOD1 mice, which express at high
      levels and develop paralysis by 6 months of age, were resistant to seeding
      by homogenates from paralyzed G93A mice. A line of G93A mice that express
      at very low levels (VLE-G93A) were responsive to seeding but at low
      efficiency. The poor susceptibility of G93A-SOD1 mice to seeding was not
      what we expected if prion-like propagation is essential to SOD1 ALS
      pathogenesis. In our prior, studies seeding homogenates from paralyzed
      G93A-SOD1 mice were injected into the spine of newborn mice, leading us to
      question whether older G93A SOD1 mice might be more susceptible to
      seeding. Here, we establish that adult VLE G93A SOD1 mice (up to 12 months
      of age) injected intrathecally with seeding homogenates containing
      misfolded G93A or G85R SOD1 developed accelerated motor neuron disease
      efficiently. Thus, we demonstrate that both the route and age of
      inoculation can influence the efficiency of SOD1 seeding to induce motor
      neuron disease in VLE G93A-SOD1 mice. These data, together with our
      earlier reports, suggest that prion-like templating contributes to disease
      progression in SOD1-ALS.
    </description>
    <description descriptionType="Methods">
      &lt;p&gt;&lt;em&gt;&lt;strong&gt;Study
      Design&lt;/strong&gt;&lt;/em&gt;&lt;/p&gt; &lt;p&gt;The primary goal of
      the study was to determine whether older VLE G93A-SOD1 mice may be more
      susceptible to prion-like seeding by intrathecal administration of seeding
      homogenates. We injected VLE G93A mice with homogenates from paralyzed
      G93A SOD1 mice or pooled homogenates from paralyzed mice expressing
      G85R-SOD1 fused to yellow fluorescent protein (YFP) that had been made
      paralyzed by injection of homogenates from paralyzed G93A SOD1 mice
      (designated P2-G93A seeding pool). Initially, we challenged 12-month-old
      mice with both seeding homogenates and then subsequently determined
      whether younger VLE G93A-SOD1 mice were also susceptible, using the
      P2-G93A pool. Controls for these experiments included uninjected VLE
      G93A-SOD1 mice and mice injected with a pooled homogenate from young
      asymptomatic naïve 2-month-old Line 230 mice (Y230 pool). Pathological
      analyses of these animals produced image data for Figures 2 - 4 of the
      accompanying publication.&lt;/p&gt; &lt;p&gt;In prelude to the G93A mouse
      study, we first sought to compare the relative seeding efficacy of
      different injection routes using a highly permissive line of homozygous
      mice expressing G85R-SOD1:YFP (Line 230 model). The seeding-homogenates
      for these studies were either the control  Y230-pool or a pool of
      homogenates from seeded paralyzed G85R-SOD1:YFP mice. We compared the
      incubation time to paralysis following lumbar intraspinal (ISP) injection
      in P0 newborn mice to lumbar intrathecal injection in 2 month old Line 230
      mice. Pathological analyses of these animals produced image data for
      Figure 1 of the accompanying publication.&lt;/p&gt; &lt;p&gt;           
      For most of these injections, we sought to inject mice from 2 to 3
      litters. The homozygous 230 mice naturally produced larger cohorts. In the
      VLE G93A study, each cohort receiving the G93A or P2-G93A pool was between
      8 and 10 transgene positive animals. Only one litter of VLE G93A mice was
      injected with the 230 pool and Y230 pool inoculum, producing smaller
      cohorts of transgene positive animals.&lt;/p&gt; &lt;p&gt;            The
      primary humane endpoint for all injections was overt paralysis of at least
      one hindlimb. In a subset of mice, other body condition factors such as
      hydration or muscle atrophy were considered in decisions to euthanize and
      harvest tissues from mice that were obviously weak but not yet paralyzed.
      Laboratory staff were not blind to treatment or genotype, but veterinary
      staff that assisted in determining endpoint were blind. Mice that were
      recommended for euthanasia due to fighting wounds or tumor development
      were excluded from the study. No mice that received seeding injections for
      data in Figure 1 were excluded from the study. For the data in Figure 2,
      there were mice that had to be excluded post-injection; Group 1 – 2 mice
      and Group 3 – 1 mouse.  &lt;/p&gt; &lt;p&gt;            Mice that were
      identified as distressed and recommended for euthanasia but did not
      otherwise exhibit signs of paralysis were excluded from statistical
      analyses of incubation periods. For data in Figure 2B there were the
      following exclusions: Group 1 – 1 animal, Group 2 – 2 animals, Group 3 – 1
      animal, Group 5 – 2 animals, Group 7 – 3 animals.   &lt;/p&gt;
      &lt;p&gt;&lt;em&gt;&lt;strong&gt;Neuropathology&lt;/strong&gt;&lt;/em&gt;&lt;/p&gt; &lt;p&gt;Detailed methods for tissue collection and neuropathology are described in Ayers et al &lt;sup&gt;23&lt;/sup&gt;. For imaging YFP fluorescence, paraffin-embedded tissues were cut at 5 μm thickness and then deparaffinized and mounted on coverslips with water; images were visualized by epifluorescence Olympus BX60 microscope. After imaging, the coverslips were removed, and the slides were stained by Campbell-Switzer silver stain as previously described &lt;sup&gt;23,34&lt;/sup&gt;. Ubiquitin staining was performed as described previously &lt;sup&gt;39&lt;/sup&gt;. Paraffin sections were deparaffinized, steamed in citrate buffer (10 mM citrate, pH 6.0, with 0.05% Tween-20) for 20 minutes. After rinsing the slides with tap water, the slides were blocked with 3% normal goat serum in PBS containing Tween 20, and then incubated overnight with ubiquitin antibody (mouse monoclonal MCA-Ubi-1, 1:1000; Encor Biotechnology, Gainesville, FL). After quenching with 0.03% hydrogen peroxide in PBS-T, sections were treated with a biotinylated anti-mouse secondary antibody (1:500; Vector Laboratories, Newark, CA), followed by ABC-HRP and DAB (KPL, Seracare, MD) detection. Slides were counterstained with hematoxylin. Images were acquired at 20× using an Aperio ScanScope XT (Leica Biosystems), processed in ImageScope, and edited in GIMP ((GNU Image Manipulation Program v3.0.4: gimp.org) for cropping and scale bar addition based on pixel calibration.&lt;/p&gt;
    </description>
    <description descriptionType="TechnicalInfo">
      # Data from: Efficient induction of motor neuron disease in transgenic
      G93A SOD1 mice by prion-like seeding This repository contains animal
      metadata and histological image data used in the study.\ All files are
      organized to ensure **clear traceability between animals, experimental
      conditions referenced in the manuscript, and figure panels**, while
      supporting **efficient data access, sharing, and repository submission**.
      --- ## Animal Metadata The image files are documentation of pathologic
      features in mice that develop paralysis as a result of the injection of
      tissue homogenates from paralyzed mice. All animals used in this study are
      documented an Excel file: * **Xu_et_al_mouse_list.xlsx** This Excel file
      contains **11 tabs**, each corresponding to animals contributing to a
      specific figure in the manuscript. The Tab names refer to the animals used
      in generating each figure in publication and the tissue homogenate used to
      induce early paralysis, listed below. The animals used in Figures 3 and 4
      are the same as those used in Figure 2; tissue sections have been stained
      by different methods. **Figure 1A-D (YFP), Figure 1B’-D’ (silver
      staining): Tab names** ·                     Fig1 P0 ISP Y230
      ·                     Fig1 P0 ISP 230 pool ·                     Fig 1
      Int-230 pool @2mo --- **Figure 2, Figure 3 (silver staining), Figure 4
      (Ubi-1 staining)**: **Tab names** ·                     Fig 2A Uninjected
      ·                     Fig 2B G93A 12mo ·                     Fig 2B
      P2-G93A 12mo ·                     Fig 2B P2-G93A P0 ·                    
      Fig 2B P2-G93A 2mo ·                     Fig 2B P2-G93A 6mo
      ·                     Fig 2B 230 pool 12mo ·                     Fig 2B
      Y230 12mo **Description of column names within each tab.** MouseID - Each
      animal receives a **mouse cage ID** (assigned by the University of Florida
      Animal Care Services) and **tail tattoo number** (1-5 per cage). Together
      these numbers create a mouse identification number (). Sex of the animal
      is indicated. Age indicates the cumulative age at the time of euthanasia.
      Symptoms - notes on clinical evaluation at time of euthanasia Incubation-
      notes the the incubation period (interval between injection of homogenates
      and appearance of paralysis) in months (mo). The relative severity of
      pathologic abnormalities in spinal cord is noted by +, ++, or +++ rating
      for each of the following.  YFP aggregates refers to direct fluorescence
      of pathologic inclusions produced by mutant superoxide dismutase 1 fused
      to yellow fluorescent protein (YFP). Silver staining refers to tissues
      stained by the Campbell-Switzer method of silver staining to secondarily
      confirm pathology.  Ubi-1 refers tissues stained by antibodies to
      ubiquitin to reveal pathology in mice that express mutant superoxide
      dismutase 1 lacking the YFP tag.  n.d. indicates not done Notes -
      indicates which images were used in specific Figures in the accompanying
      publication. 
      <br/>
       --- ## File Overview --- ## Image Data Packaging Strategy All image data
      related to this study are stored in four main folders mainly according to
      the figure number. Each folder is further subdivided according to the
      experimental groups referenced in the figure panels. The data were
      compressed into four primary ZIP archives. Each ZIP archive corresponds to
      a figure (or group of figures) in the manuscript and contains subfolders
      organized by figure panel, experimental group, and time point. ·    **YFP
      images** were captured using a **fluorescence microscope** and are saved
      in **.tiff** format. ·    **Silver-stained** and **Ubi-1 immunostained**
      slides were scanned using the **Aperio digital slide scanning system**.
      These images are saved in **.svs** format and can be viewed using
      **ScanScope** (free software
      [https://www.leicabiosystems.com/us/digital-pathology/manage/aperio-imagescope/](https://www.leicabiosystems.com/us/digital-pathology/manage/aperio-imagescope/)). --- ## Zipped Image Archives * **Figure_1A-D_(YFP).zip**\ YFP fluorescence microscopy images corresponding to Figure 1A–D. * **Figure_1B’-D’_(silver_staining).zip**\ Silver-stained images corresponding to Figure 1B′–D′. * **Figure_2_and_3_(silver_staining).zip**\ Silver-stained images corresponding to Figures 2 and 3. * **Figure_4_(UBi-1_staining).zip**\ Ubi-1 immunostained images corresponding to Figure 4. --- ## Directory Structure Inside ZIP Archives Each ZIP archive preserves a consistent internal directory structure.\ The folders listed below represent **subdirectories contained within the corresponding ZIP files** and map directly to figure panels in the manuscript. ### Figure 1A–D (YFP) * `Fig 1 Int-230 pool @2mo` * `Fig1 P0 ISP 230 pool` * `Fig1 P0 ISP Y230 control` --- ### Figure 1B′–D′ (Silver Staining) * `Fig 1 Int-230 pool @2mo silver` * `Fig 1 P0 ISP 230 pool silver` * `Fig 1 P0 ISP Y230 control silver` --- ### Figures 2 and 3 (Silver Staining) * `Figure (2A, 3A) G93A Uninjected` * `Figure (2B-1, 3D) G93A G93A@12mo` * `Figure (2B-2, 3E) P2-G93A pool@12mo` * `Figure (2B-3, 3G) P2-G93A pool@0mo` * `Figure (2B-4, 3H) P2-G93A pool@2mo` * `Figure (2B-5, 3I) P2-G93A pool@6mo` * `Figure (2B-6, 3C) G93A 230 pool@12mo` * `Figure (2B-7, 3B) G93A Y230 pool@12mo` * `Figure (3F) G93A G93A@0mo` --- ### Figure 4 (Ubi-1 Staining) * `Figure 4A G93A Uninjected (Ubi-1)` * `Figure 4B G93A Y230 pool@12mo (Ubi-1)` * `Figure 4C G93A 230 pool@12mo (Ubi-1)` * `Figure 4D G93A G93A@12mo (Ubi-1)` * `Figure 4E P2-G93A pool@12mo (Ubi-1)` * `Figure 4F G93A G93A@0mo (Ubi-1)` * `Figure 4G P2-G93A pool@0mo (Ubi-1)` * `Figure 4H P2-G93A pool@2mo (Ubi-1)` * `Figure 4I P2-G93A pool@6mo (Ubi-1)`
    </description>
  </descriptions>
</resource>","url":"https://datadryad.org/dataset/doi:10.5061/dryad.dr7sqvbb3","contentUrl":null,"metadataVersion":0,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":18,"viewsOverTime":[{"yearMonth":"2026-03","total":0},{"yearMonth":"2026-04","total":4},{"yearMonth":"2026-05","total":6},{"yearMonth":"2026-08","total":4},{"yearMonth":"2026-09","total":4}],"downloadCount":8,"downloadsOverTime":[{"yearMonth":"2026-03","total":6},{"yearMonth":"2026-05","total":1},{"yearMonth":"2026-08","total":0},{"yearMonth":"2026-09","total":1}],"referenceCount":0,"citationCount":1,"citationsOverTime":[{"year":"2026","total":1}],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2026-04-15T12:31:12.000Z","registered":"2026-04-15T12:33:32.000Z","published":"2026","updated":"2026-04-15T12:33:32.000Z"},"relationships":{"client":{"data":{"id":"dryad.dryad","type":"clients"}},"provider":{"data":{"id":"dryad","type":"providers"}},"media":{"data":{"id":"10.5061/dryad.dr7sqvbb3","type":"media"}},"references":{"data":[]},"citations":{"data":[{"id":"10.1080/19336896.2026.2630484","type":"dois"}]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}