{"data":{"id":"10.5061/dryad.bvq83bkpz","type":"dois","attributes":{"doi":"10.5061/dryad.bvq83bkpz","prefix":"10.5061","suffix":"dryad.bvq83bkpz","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Zhang, Yu","nameType":"Personal","givenName":"Yu","familyName":"Zhang","affiliation":["Nanjing University of Chinese Medicine"],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0009-0004-1595-9035","nameIdentifierScheme":"ORCID"}]},{"name":"Xu, Qilong","nameType":"Personal","givenName":"Qilong","familyName":"Xu","affiliation":["Nanjing University of Chinese Medicine"],"nameIdentifiers":[]},{"name":"Zheng, Chengbo","nameType":"Personal","givenName":"Chengbo","familyName":"Zheng","affiliation":["Nanjing University of Chinese Medicine"],"nameIdentifiers":[]},{"name":"Li, Cunyu","nameType":"Personal","givenName":"Cunyu","familyName":"Li","affiliation":["Nanjing University of Chinese Medicine"],"nameIdentifiers":[]},{"name":"Zheng, Yunfeng","nameType":"Personal","givenName":"Yunfeng","familyName":"Zheng","affiliation":["Nanjing University of Chinese Medicine"],"nameIdentifiers":[]},{"name":"Peng, Guoping","nameType":"Personal","givenName":"Guoping","familyName":"Peng","affiliation":["Nanjing University of Chinese Medicine"],"nameIdentifiers":[]}],"titles":[{"title":"Data from: The additive effect of IgE-mediated and pseudoallergic hypersensitivity in RBL-2H3 cells and guinea pigs"}],"publisher":"Dryad","container":{},"publicationYear":2026,"subjects":[{"subject":"FOS: Basic medicine","schemeUri":"https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf","subjectScheme":"fos"},{"subject":"FOS: Basic medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Drug hypersensitivity reaction"},{"subject":"gE-mediated hypersensitivity"},{"subject":"Pseudoallergic hypersensitivity"}],"contributors":[],"dates":[{"date":"2026-01-23T19:37:03Z","dateType":"Created"},{"date":"2026-01-23T19:37:04Z","dateType":"Submitted"},{"date":"2026-02-13T00:00:00Z","dateType":"Issued"},{"date":"2026-02-13T00:00:00Z","dateType":"Available"}],"language":"en","types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsCitedBy","relatedIdentifier":"10.1371/journal.pone.0340855","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["53269 bytes"],"formats":[],"version":"4","rightsList":[{"rights":"Creative Commons Zero v1.0 Universal","rightsUri":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc0-1.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Drug hypersensitivity reactions (DHR) are commonly observed with various\n medications. However, some drugs, like Qingkailing (QKL), iodixanol, and\n vancomycin, previously classified as pseudoallergenic, do not align with\n clinical observations, creating uncertainty in understanding DHR\n mechanisms and implementing prevention. In response, our research team\n hypothesized that IgE-mediated allergy and pseudoallergic hypersensitivity\n may synergistically lead to more severe reactions. We established an\n IgE-mediated hypersensitivity model using an anti-dinitrophenyl IgE\n monoclonal antibody in RBL-2H3 cells and employed ovalbumen (OVA) for\n guinea pigs. Compound 48/80 served as a positive control for\n non-immunologic hypersensitivity. We measured β-hexosaminidase and\n histamine released in cells and guinea pigs to focus on hypersensitivity\n responses from both IgE-mediated and pseudoallergic pathways. Results\n indicated that combining pseudoallergenic drugs and IgE significantly\n increased the hypersensitivity response compared to IgE or separate\n compound 48/80 groups. This effect was mirrored in the OVA-induced model.\n Notably, the three pseudoallergic drugs exhibited a similar increase in\n IgE-mediated hypersensitivity. These findings highlight the additive\n effects of IgE-mediated and pseudoallergic hypersensitivity, suggesting a\n more potent DHR response. The study also measured several cytokines and\n developed a method to differentiate between allergic, pseudoallergic DHRs,\n and additive reactions, aiding in understanding and controlling serious\n DHR risks in clinical practice.","descriptionType":"Abstract"},{"description":"# Data from: The additive effect of IgE-mediated and pseudoallergic\n hypersensitivity in RBL-2H3 cells and guinea pigs Dataset DOI:\n [10.5061/dryad.bvq83bkpz](https://doi.org/10.5061/dryad.bvq83bkpz) ##\n Description of the data and file structure This dataset supports the\n research article titled \"The additive effect of IgE-mediated and\n pseudoallergic hypersensitivity in RBL-2H3 cells and guinea pigs\"\n (PLOS ONE Manuscript ID: PONE-D-25-28449R2), authored by Yu Zhang, Chengbo\n Zheng, Qilong Xu, Cunyu Li, Yunfeng Zheng, and Guoping Peng. ### Files and\n variables #### File: DATA_PONED2528449R2_YuZhang_20260124.xlsx\n **Description:** In vitro experiments were conducted using RBL-2H3 cells,\n a widely used model for studying allergic responses. The dataset includes\n key measurements such as cell degranulation rates (assessed by\n β-hexosaminidase release assay), levels of inflammatory cytokines (e.g.,\n IL-4, TNF-α) detected via ELISA, and cell viability data. These data\n reflect the response of RBL-2H3 cells to stimuli related to IgE-mediated\n and pseudoallergic pathways, as well as their combined effects. In vitro\n experiments were conducted using RBL-2H3 cells, a widely used model for\n studying allergic responses. The dataset includes key measurements such as\n cell degranulation rates (assessed by β-hexosaminidase release assay),\n levels of inflammatory cytokines (e.g., IL-4, TNF-α) detected via ELISA,\n and cell viability data. These data reflect the response of RBL-2H3 cells\n to stimuli related to IgE-mediated and pseudoallergic pathways, as well as\n their combined effects. In vivo experiments were performed on guinea pigs,\n which were divided into appropriate groups based on experimental design.\n The dataset contains physiological and biochemical data from guinea pigs,\n including changes in body temperature, skin reaction scores (assessing\n allergic edema and erythema), serum levels of histamine and specific IgE,\n and pathological observations of relevant tissues. These data provide\n direct evidence for the additive effect of the two hypersensitivity types\n in a mammalian model. All data have been de-identified and processed in\n compliance with relevant ethical and scientific standards. Detailed\n experimental protocols, including cell culture conditions, stimulus\n concentrations, animal handling procedures, and detection methods, are\n described in the accompanying research article. This dataset is essential\n for replicating the study's findings and can serve as a valuable\n resource for other researchers investigating allergic and pseudoallergic\n mechanisms, as well as their interactions. This dataset contains the\n source data for the figures included in the main article, which were\n generated from both in vitro and in vivo experiments designed to\n investigate the additive effects of IgE-mediated and pseudoallergic\n hypersensitivity. ##### Variables * Control/Untreated cells/animals\n (negative control) * IgE, Cells treated with IgE + DNP-BSA (positive\n control for allergic activation) * C48/80, Cells treated with compound\n 48/80 (positive control for pseudoallergic activation) * OVA, Guinea pigs\n sensitized with ovalbumin (OVA) * QKL/Iodi/Van-treatedCells/animals\n treated with test compounds (Qingkailing Injection, \\ Iodixanol,\n vancomycin) * β-Hex, β-HexosaminidaseLysosomal enzyme,relative rate% *\n IgE, Immunoglobulin E,, (ng/ml) * His, Histamine, relative rate% *\n IL-4,Interleukin4,(pg/ml) * IL-13,Interleukin13, (pg/ml) * IL-6,\n Interleukin6, (ng/ml) * C3a, Complement Component 3, (ng/ml) *\n C5a, Complement Component 5, (ng/ml) * SC5b-9, Terminal Complement\n Complex, TCC, (ng/ml) ## Code/software The data is stored in Microsoft\n Excel format (.xlsx) containing 14 worksheets corresponding to different\n figures (Fig1-Fig10, S1-S6 Fig). **Free/open software for viewing:** -\n LibreOffice Calc (version 7.0 or higher) - Apache OpenOffice Calc (version\n 4.1 or higher) - Google Sheets (latest online version) **For advanced\n analysis:** - Python 3.8+ with packages: pandas (1.0+), openpyxl (3.0+),\n matplotlib/seaborn - Workflow: Read specific sheets using\n pandas.read_excel(), perform data cleaning/analysis, and reproduce figures\n with visualization libraries. No additional code or scripts are required\n for basic data viewing. ## Access information Other publicly accessible\n locations of the data: * This dataset is only publicly accessible via\n Dryad, with no additional copies hosted on other public databases. Data\n was derived from the following sources: * This dataset is original raw\n data generated directly by the authors through laboratory experiments. No\n part of this data was derived from pre-existing public datasets or other\n external sources.","descriptionType":"TechnicalInfo"}],"geoLocations":[],"fundingReferences":[{"schemeUri":"https://ror.org","funderName":"China Center of Advanced Science and Technology","awardNumber":"82074006","funderIdentifier":"https://ror.org/02egfyg20","funderIdentifierType":"ROR"}],"xml":"<?xml version="1.0" encoding="UTF-8"?>
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  <identifier identifierType="DOI">10.5061/DRYAD.BVQ83BKPZ</identifier>
  <creators>
    <creator>
      <creatorName nameType="Personal">Zhang, Yu</creatorName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0009-0004-1595-9035</nameIdentifier>
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    </creator>
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      <creatorName nameType="Personal">Xu, Qilong</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/04523zj19" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Nanjing University of Chinese Medicine</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Zheng, Chengbo</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/04523zj19" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Nanjing University of Chinese Medicine</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Li, Cunyu</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/04523zj19" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Nanjing University of Chinese Medicine</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Zheng, Yunfeng</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/04523zj19" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Nanjing University of Chinese Medicine</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Peng, Guoping</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/04523zj19" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Nanjing University of Chinese Medicine</affiliation>
    </creator>
  </creators>
  <titles>
    <title>
      Data from: The additive effect of IgE-mediated and pseudoallergic
      hypersensitivity in RBL-2H3 cells and guinea pigs
    </title>
  </titles>
  <publisher publisherIdentifier="https://ror.org/00x6h5n95" publisherIdentifierScheme="ROR" schemeURI="https://ror.org/">Dryad</publisher>
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    <subject subjectScheme="fos" schemeURI="https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf">FOS: Basic medicine</subject>
    <subject>Drug hypersensitivity reaction</subject>
    <subject>gE-mediated hypersensitivity</subject>
    <subject>Pseudoallergic hypersensitivity</subject>
  </subjects>
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      <funderName>China Center of Advanced Science and Technology</funderName>
      <funderIdentifier funderIdentifierType="ROR">https://ror.org/02egfyg20</funderIdentifier>
      <awardNumber>82074006</awardNumber>
      <awardTitle/>
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    <date dateType="Created">2026-01-23T19:37:03Z</date>
    <date dateType="Submitted">2026-01-23T19:37:04Z</date>
    <date dateType="Issued">2026-02-13T00:00:00Z</date>
    <date dateType="Available">2026-02-13T00:00:00Z</date>
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  <descriptions>
    <description descriptionType="Abstract">
      Drug hypersensitivity reactions (DHR) are commonly observed with various
      medications. However, some drugs, like Qingkailing (QKL), iodixanol, and
      vancomycin, previously classified as pseudoallergenic, do not align with
      clinical observations, creating uncertainty in understanding DHR
      mechanisms and implementing prevention. In response, our research team
      hypothesized that IgE-mediated allergy and pseudoallergic hypersensitivity
      may synergistically lead to more severe reactions. We established an
      IgE-mediated hypersensitivity model using an anti-dinitrophenyl IgE
      monoclonal antibody in RBL-2H3 cells and employed ovalbumen (OVA) for
      guinea pigs. Compound 48/80 served as a positive control for
      non-immunologic hypersensitivity. We measured β-hexosaminidase and
      histamine released in cells and guinea pigs to focus on hypersensitivity
      responses from both IgE-mediated and pseudoallergic pathways. Results
      indicated that combining pseudoallergenic drugs and IgE significantly
      increased the hypersensitivity response compared to IgE or separate
      compound 48/80 groups. This effect was mirrored in the OVA-induced model.
      Notably, the three pseudoallergic drugs exhibited a similar increase in
      IgE-mediated hypersensitivity. These findings highlight the additive
      effects of IgE-mediated and pseudoallergic hypersensitivity, suggesting a
      more potent DHR response. The study also measured several cytokines and
      developed a method to differentiate between allergic, pseudoallergic DHRs,
      and additive reactions, aiding in understanding and controlling serious
      DHR risks in clinical practice.
    </description>
    <description descriptionType="TechnicalInfo"><br/>
       # Data from: The additive effect of IgE-mediated and pseudoallergic
      hypersensitivity in RBL-2H3 cells and guinea pigs Dataset DOI:
      [10.5061/dryad.bvq83bkpz](https://doi.org/10.5061/dryad.bvq83bkpz) ##
      Description of the data and file structure This dataset supports the
      research article titled "The additive effect of IgE-mediated and
      pseudoallergic hypersensitivity in RBL-2H3 cells and guinea pigs"
      (PLOS ONE Manuscript ID: PONE-D-25-28449R2), authored by Yu Zhang, Chengbo
      Zheng, Qilong Xu, Cunyu Li, Yunfeng Zheng, and Guoping Peng. ### Files and
      variables #### File: DATA_PONED2528449R2_YuZhang_20260124.xlsx
      **Description:** In vitro experiments were conducted using RBL-2H3 cells,
      a widely used model for studying allergic responses. The dataset includes
      key measurements such as cell degranulation rates (assessed by
      β-hexosaminidase release assay), levels of inflammatory cytokines (e.g.,
      IL-4, TNF-α) detected via ELISA, and cell viability data. These data
      reflect the response of RBL-2H3 cells to stimuli related to IgE-mediated
      and pseudoallergic pathways, as well as their combined effects. In vitro
      experiments were conducted using RBL-2H3 cells, a widely used model for
      studying allergic responses. The dataset includes key measurements such as
      cell degranulation rates (assessed by β-hexosaminidase release assay),
      levels of inflammatory cytokines (e.g., IL-4, TNF-α) detected via ELISA,
      and cell viability data. These data reflect the response of RBL-2H3 cells
      to stimuli related to IgE-mediated and pseudoallergic pathways, as well as
      their combined effects. In vivo experiments were performed on guinea pigs,
      which were divided into appropriate groups based on experimental design.
      The dataset contains physiological and biochemical data from guinea pigs,
      including changes in body temperature, skin reaction scores (assessing
      allergic edema and erythema), serum levels of histamine and specific IgE,
      and pathological observations of relevant tissues. These data provide
      direct evidence for the additive effect of the two hypersensitivity types
      in a mammalian model. All data have been de-identified and processed in
      compliance with relevant ethical and scientific standards. Detailed
      experimental protocols, including cell culture conditions, stimulus
      concentrations, animal handling procedures, and detection methods, are
      described in the accompanying research article. This dataset is essential
      for replicating the study's findings and can serve as a valuable
      resource for other researchers investigating allergic and pseudoallergic
      mechanisms, as well as their interactions. This dataset contains the
      source data for the figures included in the main article, which were
      generated from both in vitro and in vivo experiments designed to
      investigate the additive effects of IgE-mediated and pseudoallergic
      hypersensitivity. ##### Variables * Control/Untreated cells/animals
      (negative control) * IgE, Cells treated with IgE + DNP-BSA (positive
      control for allergic activation) * C48/80, Cells treated with compound
      48/80 (positive control for pseudoallergic activation) * OVA, Guinea pigs
      sensitized with ovalbumin (OVA) * QKL/Iodi/Van-treatedCells/animals
      treated with test compounds (Qingkailing Injection, \ Iodixanol,
      vancomycin) * β-Hex, β-HexosaminidaseLysosomal enzyme,relative rate% *
      IgE, Immunoglobulin E,, (ng/ml) * His, Histamine, relative rate% *
      IL-4,Interleukin4,(pg/ml) * IL-13,Interleukin13, (pg/ml) * IL-6,
      Interleukin6, (ng/ml) * C3a, Complement Component 3, (ng/ml) *
      C5a, Complement Component 5, (ng/ml) * SC5b-9, Terminal Complement
      Complex, TCC, (ng/ml) ## Code/software The data is stored in Microsoft
      Excel format (.xlsx) containing 14 worksheets corresponding to different
      figures (Fig1-Fig10, S1-S6 Fig). **Free/open software for viewing:** -
      LibreOffice Calc (version 7.0 or higher) - Apache OpenOffice Calc (version
      4.1 or higher) - Google Sheets (latest online version) **For advanced
      analysis:** - Python 3.8+ with packages: pandas (1.0+), openpyxl (3.0+),
      matplotlib/seaborn - Workflow: Read specific sheets using
      pandas.read_excel(), perform data cleaning/analysis, and reproduce figures
      with visualization libraries. No additional code or scripts are required
      for basic data viewing. ## Access information Other publicly accessible
      locations of the data: * This dataset is only publicly accessible via
      Dryad, with no additional copies hosted on other public databases. Data
      was derived from the following sources: * This dataset is original raw
      data generated directly by the authors through laboratory experiments. No
      part of this data was derived from pre-existing public datasets or other
      external sources.
    </description>
  </descriptions>
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