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The goal of this study was to explore the role of NHE6 in\n bone homeostasis. To this end, we studied the bone phenotype of NHE6\n knock-out mice by microcomputed tomography, quantitative histomorphometry\n and complementary ex vivo and in vitro studies. We detected NHE6\n transcript and protein in both differentiated osteoclasts and mineralizing\n osteoblasts. In vitro studies with osteoclasts and osteoblasts derived\n from NHE6 knock-out mice demonstrated normal osteoclast differentiation\n and osteoblast proliferation without an impairment in mineralization\n capacity. Microcomputed tomography and bone histomorphometry studies\n showed a significantly reduced bone volume and trabecular number as well\n as an increased trabecular space at lumbar vertebrae of 6 months old NHE6\n knock-out mice. The bone degradation marker c-terminal telopeptides of\n type I collagen was unaltered in NHE6 knock-out mice. However, we observed\n a reduction of the bone formation marker procollagen type 1 N-terminal\n propeptide, and increased circulating sclerostin levels in NHE6 knock-out\n mice. Subsequent studies revealed a significant upregulation of sclerostin\n transcript expression in both primary calvarial cultures and femora\n derived from NHE6 knock-out mice. Thus, loss of NHE6 in mice causes an\n increase of sclerostin expression associated with reduced bone formation\n and low bone volume.","descriptionType":"Abstract"},{"description":"See detailed information in published manuscript\n (https://pubmed.ncbi.nlm.nih.gov/34508879/) and attached README\n file.","descriptionType":"Methods"},{"description":"See detailed information in attached README file.","descriptionType":"Other"}],"geoLocations":[],"fundingReferences":[{"schemeUri":"https://ror.org","funderName":"Swiss National Science Foundation","awardNumber":"31003A_152829","funderIdentifier":"https://ror.org/00yjd3n13","funderIdentifierType":"ROR"},{"schemeUri":"https://ror.org","funderName":"Swiss National Science Foundation","awardNumber":"31003A_152829","funderIdentifier":"https://ror.org/00yjd3n13","funderIdentifierType":"ROR"},{"schemeUri":"https://ror.org","funderName":"Swiss National Science Foundation","awardNumber":"National Center for Competence in Research (NCCR) TransCure","funderIdentifier":"https://ror.org/00yjd3n13","funderIdentifierType":"ROR"}],"xml":"<?xml version="1.0" encoding="UTF-8"?>
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    <title>
      Deletion of the sodium/hydrogen exchanger 6 causes low bone volume in
      adult mice
    </title>
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    <description descriptionType="Abstract">
      The sodium/hydrogen exchanger 6 (NHE6) localizes to recycling endosomes,
      where it mediates endosomal alkalinization through K+/H+ exchange.
      Mutations in the SLC9A6 gene encoding NHE6 cause severe X-linked mental
      retardation, epilepsy, autism and corticobasal degeneration in humans.
      Patients with SLC9A6 mutations exhibit skeletal malformations, and a
      previous study suggested a key role of NHE6 in osteoblast-mediated
      mineralization. The goal of this study was to explore the role of NHE6 in
      bone homeostasis. To this end, we studied the bone phenotype of NHE6
      knock-out mice by microcomputed tomography, quantitative histomorphometry
      and complementary ex vivo and in vitro studies. We detected NHE6
      transcript and protein in both differentiated osteoclasts and mineralizing
      osteoblasts. In vitro studies with osteoclasts and osteoblasts derived
      from NHE6 knock-out mice demonstrated normal osteoclast differentiation
      and osteoblast proliferation without an impairment in mineralization
      capacity. Microcomputed tomography and bone histomorphometry studies
      showed a significantly reduced bone volume and trabecular number as well
      as an increased trabecular space at lumbar vertebrae of 6 months old NHE6
      knock-out mice. The bone degradation marker c-terminal telopeptides of
      type I collagen was unaltered in NHE6 knock-out mice. However, we observed
      a reduction of the bone formation marker procollagen type 1 N-terminal
      propeptide, and increased circulating sclerostin levels in NHE6 knock-out
      mice. Subsequent studies revealed a significant upregulation of sclerostin
      transcript expression in both primary calvarial cultures and femora
      derived from NHE6 knock-out mice. Thus, loss of NHE6 in mice causes an
      increase of sclerostin expression associated with reduced bone formation
      and low bone volume.
    </description>
    <description descriptionType="Methods">
      &lt;p&gt;See detailed information in published manuscript
      (https://pubmed.ncbi.nlm.nih.gov/34508879/) and attached README
      file.&lt;/p&gt;
    </description>
    <description descriptionType="Other">&lt;p&gt;See detailed information in attached README file.&lt;/p&gt;</description>
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