{"data":{"id":"10.5061/dryad.9ghx3ffrh","type":"dois","attributes":{"doi":"10.5061/dryad.9ghx3ffrh","prefix":"10.5061","suffix":"dryad.9ghx3ffrh","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Jeoh, Tina","nameType":"Personal","givenName":"Tina","familyName":"Jeoh","affiliation":["University of California, Davis"],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0000-0002-0727-4237","nameIdentifierScheme":"ORCID"}]},{"name":"Blossom, Benedikt","nameType":"Personal","givenName":"Benedikt","familyName":"Blossom","affiliation":[","],"nameIdentifiers":[]},{"name":"Goodwin, Peter","nameType":"Personal","givenName":"Peter","familyName":"Goodwin","affiliation":["Los Alamos National Laboratory"],"nameIdentifiers":[]}],"titles":[{"title":"Single molecule image sequences of Thermothielavioides terrestris AA9E (TtAA9E) on cellulose in oxygen scavenging systems"}],"publisher":"Dryad","container":{},"publicationYear":2025,"subjects":[{"subject":"FOS: Industrial biotechnology","schemeUri":"https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf","subjectScheme":"fos"},{"subject":"FOS: Industrial biotechnology","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Nano-technology","schemeUri":"https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf","subjectScheme":"fos"},{"subject":"FOS: Engineering and technology","schemeUri":"https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf","subjectScheme":"fos"},{"subject":"FOS: Engineering and technology","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"total internal reflection fluorescence microscopy"},{"subject":"TIRFM"},{"subject":"Lytic polysaccharide Monooxygenase"},{"subject":"LPMO"},{"subject":"Cellulose","schemeUri":"https://github.com/PLOS/plos-thesaurus","subjectScheme":"PLOS Subject Area Thesaurus"},{"subject":"Single molecule imaging"}],"contributors":[{"name":"Center for Integrated Nanotechnologies, Los Alamos National Laboratory","nameType":"Personal","givenName":"Los Alamos National Laboratory","familyName":"Center for Integrated Nanotechnologies","affiliation":[],"contributorType":"Sponsor","nameIdentifiers":[]}],"dates":[{"date":"2025-07-14T21:03:46Z","dateType":"Created"},{"date":"2025-09-30T22:54:34Z","dateType":"Submitted"},{"date":"2025-10-20T00:00:00Z","dateType":"Issued"},{"date":"2025-10-20T00:00:00Z","dateType":"Available"}],"language":"en","types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsCitedBy","relatedIdentifier":"10.1002/bit.70080","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["8268891368 bytes"],"formats":[],"version":"5","rightsList":[{"rights":"Creative Commons Zero v1.0 Universal","rightsUri":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc0-1.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Purified and Cy5-labeled lytic polysaccharide monooxygenase (LPMO)\n Thermothielavioides terrestris AA9E (TtAA9E) interacting at the surface of\n crystalline cellulose fibrils were imaged by Total Internal Reflectance\n Fluorescence Microscopy (TIRFM) in two different oxygen scavenging\n systems: Glucose Oxidase-Catalase (GODCAT) and Protocatechuic\n Acid/Protocatechuate-3,4-dioxygenase (PCA/PCD). The datasets (movies) show\n how oxygen and hydrogen peroxide availability, and reductant\n type/availability impact specific binding of the LPMO to highly ordered\n cellulose fibrils.","descriptionType":"Abstract"},{"description":"# Single Molecule Image Sequences of *Thermothielavioides terrestris* AA9E\n (*Tt*AA9E) on Cellulose in Oxygen Scavenging System Dataset DOI:\n [10.5061/dryad.9ghx3ffrh](10.5061/dryad.9ghx3ffrh) Single-molecule\n fluorescence images of individual Cy5-labeled enzyme molecules binding and\n unbinding from cellulose fibrils were imaged using 488 nm and 637 nm\n excitation laser reflected by a multiline dichroic mirror (FF500/646-Di01,\n Semrock) and focused at the back aperture of a 1.49 NA 60X oil-immersion\n objective (Olympus) to provide total-internal-reflection (TIR) excitation\n at the cover glass / water interface across a ~50 µm diameter area in the\n object plane. Sample emission was collected and imaged by the same\n objective onto the 512x512-pixel sensor of an electron multiplying CCD\n (EMCCD) camera (Photonmax, Princeton Instruments). A 37 nm wide bandpass\n filter centered at 676 nm was used to isolate Cy5 fluorescence excited at\n 637 nm. For the binding time measurements, the overall magnification of\n the imaging system (73X) mapped each EMCCD pixel to a 220x220 nm area in\n the object plane.  Relatively low power (~0.2 mW @ 637 nm) excitation was\n used to reduce the effect of Cy5 photobleaching on the binding time\n measurements. Under similar imaging conditions, fluorescence lifetimes of\n most (90%) of Cy5 fluorophores exhibited an extended characteristic decay\n time of 1101 s in the GODCAT oxygen scavenging buffer system (Supplemental\n Information 1). Image stacks were collected at EMCCD camera integration\n times of 1 second. The table below indicate enzyme and reaction conditions\n associated with each datafile. ## Description of the data and file\n structure Descriptions of the data and file structure are provided in the\n attached Datafile_structure.csv ## Code/software Both .spe and .tif files\n contain image stacks (multiple images collected in sequence over a period\n of time) that can be directly opened in ImageJ (File-\u0026gt;Open and browse\n to select file, or drag and drop). The image stacks can be played to watch\n movies of fluorescently-labeled cellulose binding and unbinding on the\n surface with cellulose fibrils. It is helpful to match brightness and\n contrast of the images (Image-\u0026gt;Adjust-\u0026gt;Brightness/Contrast) when\n comparing multiple data sets.","descriptionType":"TechnicalInfo"}],"geoLocations":[],"fundingReferences":[{"schemeUri":"https://ror.org","funderName":"United States Department of Energy","funderIdentifier":"https://ror.org/01bj3aw27","funderIdentifierType":"ROR"},{"schemeUri":"https://ror.org","funderName":"Novo Nordisk Foundation","funderIdentifier":"https://ror.org/04txyc737","funderIdentifierType":"ROR"}],"xml":"<?xml version="1.0" encoding="UTF-8"?>
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      <creatorName nameType="Personal">Jeoh, Tina</creatorName>
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      <creatorName nameType="Personal">Goodwin, Peter</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/01e41cf67" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Los Alamos National Laboratory</affiliation>
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  <titles>
    <title>
      Single molecule image sequences of Thermothielavioides terrestris AA9E
      (TtAA9E) on cellulose in oxygen scavenging systems
    </title>
  </titles>
  <publisher publisherIdentifier="https://ror.org/00x6h5n95" publisherIdentifierScheme="ROR" schemeURI="https://ror.org/">Dryad</publisher>
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    <subject subjectScheme="fos" schemeURI="https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf">FOS: Industrial biotechnology</subject>
    <subject subjectScheme="fos" schemeURI="https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf">FOS: Nano-technology</subject>
    <subject subjectScheme="fos" schemeURI="https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf">FOS: Engineering and technology</subject>
    <subject>total internal reflection fluorescence microscopy</subject>
    <subject>TIRFM</subject>
    <subject>Lytic polysaccharide Monooxygenase</subject>
    <subject>LPMO</subject>
    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Cellulose</subject>
    <subject>Single molecule imaging</subject>
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      <funderName>United States Department of Energy</funderName>
      <funderIdentifier funderIdentifierType="ROR">https://ror.org/01bj3aw27</funderIdentifier>
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  <dates>
    <date dateType="Created">2025-07-14T21:03:46Z</date>
    <date dateType="Submitted">2025-09-30T22:54:34Z</date>
    <date dateType="Issued">2025-10-20T00:00:00Z</date>
    <date dateType="Available">2025-10-20T00:00:00Z</date>
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  <descriptions>
    <description descriptionType="Abstract">
      Purified and Cy5-labeled lytic polysaccharide monooxygenase (LPMO)
      Thermothielavioides terrestris AA9E (TtAA9E) interacting at the surface of
      crystalline cellulose fibrils were imaged by Total Internal Reflectance
      Fluorescence Microscopy (TIRFM) in two different oxygen scavenging
      systems: Glucose Oxidase-Catalase (GODCAT) and Protocatechuic
      Acid/Protocatechuate-3,4-dioxygenase (PCA/PCD). The datasets (movies) show
      how oxygen and hydrogen peroxide availability, and reductant
      type/availability impact specific binding of the LPMO to highly ordered
      cellulose fibrils.
    </description>
    <description descriptionType="TechnicalInfo">
      # Single Molecule Image Sequences of *Thermothielavioides terrestris* AA9E
      (*Tt*AA9E) on Cellulose in Oxygen Scavenging System Dataset DOI:
      [10.5061/dryad.9ghx3ffrh](10.5061/dryad.9ghx3ffrh) Single-molecule
      fluorescence images of individual Cy5-labeled enzyme molecules binding and
      unbinding from cellulose fibrils were imaged using 488 nm and 637 nm
      excitation laser reflected by a multiline dichroic mirror (FF500/646-Di01,
      Semrock) and focused at the back aperture of a 1.49 NA 60X oil-immersion
      objective (Olympus) to provide total-internal-reflection (TIR) excitation
      at the cover glass / water interface across a ~50 µm diameter area in the
      object plane. Sample emission was collected and imaged by the same
      objective onto the 512x512-pixel sensor of an electron multiplying CCD
      (EMCCD) camera (Photonmax, Princeton Instruments). A 37 nm wide bandpass
      filter centered at 676 nm was used to isolate Cy5 fluorescence excited at
      637 nm. For the binding time measurements, the overall magnification of
      the imaging system (73X) mapped each EMCCD pixel to a 220x220 nm area in
      the object plane.  Relatively low power (~0.2 mW @ 637 nm) excitation was
      used to reduce the effect of Cy5 photobleaching on the binding time
      measurements. Under similar imaging conditions, fluorescence lifetimes of
      most (90%) of Cy5 fluorophores exhibited an extended characteristic decay
      time of 1101 s in the GODCAT oxygen scavenging buffer system (Supplemental
      Information 1). Image stacks were collected at EMCCD camera integration
      times of 1 second. The table below indicate enzyme and reaction conditions
      associated with each datafile. ## Description of the data and file
      structure Descriptions of the data and file structure are provided in the
      attached Datafile_structure.csv ## Code/software Both .spe and .tif files
      contain image stacks (multiple images collected in sequence over a period
      of time) that can be directly opened in ImageJ (File-&gt;Open and browse
      to select file, or drag and drop). The image stacks can be played to watch
      movies of fluorescently-labeled cellulose binding and unbinding on the
      surface with cellulose fibrils. It is helpful to match brightness and
      contrast of the images (Image-&gt;Adjust-&gt;Brightness/Contrast) when
      comparing multiple data sets.
    </description>
  </descriptions>
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