{"data":{"id":"10.5061/dryad.7pvmcvdx9","type":"dois","attributes":{"doi":"10.5061/dryad.7pvmcvdx9","prefix":"10.5061","suffix":"dryad.7pvmcvdx9","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Yang, Ying","nameType":"Personal","givenName":"Ying","familyName":"Yang","affiliation":["UNSW Sydney"],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0000-0001-6009-4748","nameIdentifierScheme":"ORCID"}]},{"name":"Ma, Yuanqing","nameType":"Personal","givenName":"Yuanqing","familyName":"Ma","affiliation":["UNSW Sydney"],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0000-0003-0557-4559","nameIdentifierScheme":"ORCID"}]},{"name":"Berengut, Jonathan F.","nameType":"Personal","givenName":"Jonathan F.","familyName":"Berengut","affiliation":["UNSW Sydney"],"nameIdentifiers":[]},{"name":"Lee, Lawrence K.","nameType":"Personal","givenName":"Lawrence K.","familyName":"Lee","affiliation":["UNSW Sydney"],"nameIdentifiers":[]},{"name":"Tilley, Richard","nameType":"Personal","givenName":"Richard","familyName":"Tilley","affiliation":["UNSW Sydney"],"nameIdentifiers":[]},{"name":"Gaus, Katharina","nameType":"Personal","givenName":"Katharina","familyName":"Gaus","affiliation":["UNSW Sydney"],"nameIdentifiers":[]},{"name":"Gooding, J. Justin","nameType":"Personal","givenName":"J. Justin","familyName":"Gooding","affiliation":["UNSW Sydney"],"nameIdentifiers":[]}],"titles":[{"title":"Source data for: Electrochemically controlled blinking of fluorophores for quantitative STORM imaging"}],"publisher":"Dryad","container":{},"publicationYear":2024,"subjects":[{"subject":"TIRF imaging"},{"subject":"Electrochemistry","schemeUri":"https://github.com/PLOS/plos-thesaurus","subjectScheme":"PLOS Subject Area Thesaurus"},{"subject":"STORM imaging"},{"subject":"FOS: Nano-technology","schemeUri":"https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf","subjectScheme":"fos"}],"contributors":[],"dates":[{"date":"2024-02-20T04:51:23Z","dateType":"Created"},{"date":"2024-04-06T04:48:32Z","dateType":"Submitted"},{"date":"2024-04-10T00:00:00Z","dateType":"Issued"},{"date":"2024-04-10T00:00:00Z","dateType":"Available"}],"language":"en","types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsCitedBy","relatedIdentifier":"10.1101/2023.09.13.557504","relatedIdentifierType":"DOI"},{"relationType":"IsCitedBy","relatedIdentifier":"10.1038/s41566-024-01431-0","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["3079204610 bytes"],"formats":[],"version":"6","rightsList":[{"rights":"Creative Commons Zero v1.0 Universal","rightsUri":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc0-1.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Stochastic optical reconstruction microscopy (STORM) allows widefield\n imaging with single-molecule resolution by calculating the coordinates of\n individual fluorophores from the separation of the fluorophore emission in\n both time and space. Such separation is achieved by photoswitching the\n fluorophores between a long-lived OFF state and an emissive ON state.\n While STORM can image single molecules, molecular counting remains\n challenging due to undercounting errors from photobleached or overlapping\n dyes and overcounting artifacts from the repetitive random blinking of the\n dyes. Here, we show that fluorophores can be switched electrochemically\n for STORM imaging (EC-STORM), with excellent control over the switching\n kinetics, duty cycle, and recovery yield. Using EC-STORM, we demonstrate\n molecular counting by using electrochemical potential to control the\n photophysics of dyes. The random blinking of dyes is suppressed by a\n negative potential but the switching ON event can be activated by a short\n pulsed positive potential, such that the frequency of ON events scales\n linearly with the number of underlying dyes. We also demonstrate the\n EC-STORM of tubulins in fixed cells with a spatial resolution as low as\n ~28 nm and counting of single Alexa 647 fluorophores on various DNA\n nanoruler structures. This control over fluorophore switching will enable\n EC-STORM to be broadly applicable in super-resolution imaging and\n molecular counting.","descriptionType":"Abstract"},{"description":"The TIRF images were collected on Zeiss Elyra SP2\n Super-resolution PALM microscope. The collimated and linearly p-polarized\n 642 nm laser was reflected from the 642 nm long pass dichroic mirror and\n focused at the back focal plane of the 100 X 1.46 NA Oil objective. The\n focus is laterally shifted alone in the back focal plane to provide either\n EPI (0o) or TIRF (66.7o) illumination. The TIRF angle was identical\n between the glass and ITO surface. The fluorescence was collected by the\n same objective and guided to a cooled electron-multiplying charge-coupled\n Device EMCCD camera (iXon DU-897). The localization analysis was performed\n in Zen 2.3 software (black version) and exported as PALM.txt\n files. Cyclic voltammetry, chronoamperometry, and\n different pulsed voltammetry were performed by SP-200 Potentiostat\n (Bio-Logic, France). All the raw data were exported directly from the\n software EC-Lab14.2 as txt files.","descriptionType":"Methods"},{"description":"# **Source data for: Electrochemically controlled blinking of fluorophores\n for quantitative STORM imaging** --- DOI: 10.5061/dryad.7pvmcvdx9 The\n provided data consists of the raw imaging data file and Matlab scripts\n utilized in the publication titled \"Electrochemically Controlled\n Blinking of Fluorophores for Quantitative STORM Imaging\" by Yang et\n al. (2024), published in *Nature Photonics*. A description of the data\n collection and analysis methodologies is outlined in the Methods section,\n with additional specifics available in the manuscript's Methods\n section. For further inquiries or information, please feel free to contact\n the corresponding author. ## Description of the data and file structure\n Zipped folders in the dataset: Fig. 1, Fig.2, Fig. 3, Fig. 4, SI Table 1,\n Sup Fig.2, Sup Fig.3, Sup Fig.4, Sup Fig.5, Sup Fig.6, Sup Fig.7, Sup\n Fig.8, and Sup Fig.9. The relationship between the files is structured\n according to their association with the figure numbers in the manuscript.\n Each file contains the raw data employed in generating the corresponding\n graph in the figure, along with the Matlab script utilized for analysis,\n where applicable. Further elaboration on the analysis procedures can be\n found within the Methods section of the manuscript, as well as in the\n comments section of the Matlab script.","descriptionType":"TechnicalInfo"}],"geoLocations":[],"fundingReferences":[{"schemeUri":"https://ror.org","funderName":"Australian Research Council","funderIdentifier":"https://ror.org/05mmh0f86","funderIdentifierType":"ROR"},{"schemeUri":"https://ror.org","funderName":"National Health and Medical Research Council","funderIdentifier":"https://ror.org/011kf5r70","funderIdentifierType":"ROR"}],"xml":"<?xml version="1.0" encoding="UTF-8"?>
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      <affiliation affiliationIdentifier="https://ror.org/03r8z3t63" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">UNSW Sydney</affiliation>
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  <titles>
    <title>
      Source data for: Electrochemically controlled blinking of fluorophores for
      quantitative STORM imaging
    </title>
  </titles>
  <publisher publisherIdentifier="https://ror.org/00x6h5n95" publisherIdentifierScheme="ROR" schemeURI="https://ror.org/">Dryad</publisher>
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  <publicationYear>2024</publicationYear>
  <subjects>
    <subject>TIRF imaging</subject>
    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Electrochemistry</subject>
    <subject>STORM imaging</subject>
    <subject subjectScheme="fos" schemeURI="https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf">FOS: Nano-technology</subject>
  </subjects>
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      <funderName>Australian Research Council</funderName>
      <funderIdentifier funderIdentifierType="ROR">https://ror.org/05mmh0f86</funderIdentifier>
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    <fundingReference>
      <funderName>National Health and Medical Research Council</funderName>
      <funderIdentifier funderIdentifierType="ROR">https://ror.org/011kf5r70</funderIdentifier>
    </fundingReference>
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  <dates>
    <date dateType="Created">2024-02-20T04:51:23Z</date>
    <date dateType="Submitted">2024-04-06T04:48:32Z</date>
    <date dateType="Issued">2024-04-10T00:00:00Z</date>
    <date dateType="Available">2024-04-10T00:00:00Z</date>
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  <language>en</language>
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  <descriptions>
    <description descriptionType="Abstract">
      Stochastic optical reconstruction microscopy (STORM) allows widefield
      imaging with single-molecule resolution by calculating the coordinates of
      individual fluorophores from the separation of the fluorophore emission in
      both time and space. Such separation is achieved by photoswitching the
      fluorophores between a long-lived OFF state and an emissive ON state.
      While STORM can image single molecules, molecular counting remains
      challenging due to undercounting errors from photobleached or overlapping
      dyes and overcounting artifacts from the repetitive random blinking of the
      dyes. Here, we show that fluorophores can be switched electrochemically
      for STORM imaging (EC-STORM), with excellent control over the switching
      kinetics, duty cycle, and recovery yield. Using EC-STORM, we demonstrate
      molecular counting by using electrochemical potential to control the
      photophysics of dyes. The random blinking of dyes is suppressed by a
      negative potential but the switching ON event can be activated by a short
      pulsed positive potential, such that the frequency of ON events scales
      linearly with the number of underlying dyes. We also demonstrate the
      EC-STORM of tubulins in fixed cells with a spatial resolution as low as
      ~28 nm and counting of single Alexa 647 fluorophores on various DNA
      nanoruler structures. This control over fluorophore switching will enable
      EC-STORM to be broadly applicable in super-resolution imaging and
      molecular counting.
    </description>
    <description descriptionType="Methods">
      &lt;p&gt;The TIRF images were collected on Zeiss Elyra SP2
      Super-resolution PALM microscope. The collimated and linearly p-polarized
      642 nm laser was reflected from the 642 nm long pass dichroic mirror and
      focused at the back focal plane of the 100 X 1.46 NA Oil objective. The
      focus is laterally shifted alone in the back focal plane to provide either
      EPI (0o) or TIRF (66.7o) illumination. The TIRF angle was identical
      between the glass and ITO surface. The fluorescence was collected by the
      same objective and guided to a cooled electron-multiplying charge-coupled
      Device EMCCD camera (iXon DU-897). The localization analysis was performed
      in Zen 2.3 software (black version) and exported as PALM.txt
      files.&lt;/p&gt; &lt;p&gt;Cyclic voltammetry, chronoamperometry, and
      different pulsed voltammetry were performed by SP-200 Potentiostat
      (Bio-Logic, France). All the raw data were exported directly from the
      software EC-Lab14.2 as txt files.&lt;/p&gt;
    </description>
    <description descriptionType="TechnicalInfo">
      # **Source data for: Electrochemically controlled blinking of fluorophores
      for quantitative STORM imaging** --- DOI: 10.5061/dryad.7pvmcvdx9 The
      provided data consists of the raw imaging data file and Matlab scripts
      utilized in the publication titled "Electrochemically Controlled
      Blinking of Fluorophores for Quantitative STORM Imaging" by Yang et
      al. (2024), published in *Nature Photonics*. A description of the data
      collection and analysis methodologies is outlined in the Methods section,
      with additional specifics available in the manuscript's Methods
      section. For further inquiries or information, please feel free to contact
      the corresponding author. ## Description of the data and file structure
      Zipped folders in the dataset: Fig. 1, Fig.2, Fig. 3, Fig. 4, SI Table 1,
      Sup Fig.2, Sup Fig.3, Sup Fig.4, Sup Fig.5, Sup Fig.6, Sup Fig.7, Sup
      Fig.8, and Sup Fig.9. The relationship between the files is structured
      according to their association with the figure numbers in the manuscript.
      Each file contains the raw data employed in generating the corresponding
      graph in the figure, along with the Matlab script utilized for analysis,
      where applicable. Further elaboration on the analysis procedures can be
      found within the Methods section of the manuscript, as well as in the
      comments section of the Matlab script.
    </description>
  </descriptions>
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