{"data":{"id":"10.5061/dryad.7d7wm387m","type":"dois","attributes":{"doi":"10.5061/dryad.7d7wm387m","prefix":"10.5061","suffix":"dryad.7d7wm387m","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Chaderjian, Aria S.","nameType":"Personal","givenName":"Aria S.","familyName":"Chaderjian","affiliation":["University of California, Santa Barbara"],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0009-0004-8789-0700","nameIdentifierScheme":"ORCID"}]},{"name":"Saleh, Omar A.","nameType":"Personal","givenName":"Omar A.","familyName":"Saleh","affiliation":["University of California, Santa Barbara"],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0000-0002-9197-4024","nameIdentifierScheme":"ORCID"}]}],"titles":[{"title":"Data from: Diverse, distinct, and densely packed DNA nanostar droplets"}],"publisher":"Dryad","container":{},"publicationYear":2026,"subjects":[{"subject":"FOS: Physical sciences","schemeUri":"https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf","subjectScheme":"fos"},{"subject":"FOS: Physical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Materials engineering","schemeUri":"https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf","subjectScheme":"fos"},{"subject":"FOS: Materials engineering","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Chemical engineering","schemeUri":"https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf","subjectScheme":"fos"},{"subject":"FOS: Chemical engineering","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Nano-technology","schemeUri":"https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf","subjectScheme":"fos"},{"subject":"DNA self-assembly"},{"subject":"biomolecular liquid"},{"subject":"condensate"},{"subject":"jammed system"},{"subject":"glassy dynamics"},{"subject":"Liquid-liquid phase separation"}],"contributors":[{"name":"University of California, Santa Barbara","nameType":"Personal","givenName":"Santa Barbara","familyName":"University of California","affiliation":[],"contributorType":"Sponsor","nameIdentifiers":[]}],"dates":[{"date":"2025-08-22T16:12:27Z","dateType":"Created"},{"date":"2026-01-30T16:15:14Z","dateType":"Submitted"},{"date":"2026-02-03T00:00:00Z","dateType":"Issued"},{"date":"2026-02-03T00:00:00Z","dateType":"Available"}],"language":"en","types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsCitedBy","relatedIdentifier":"10.48550/arxiv.2508.18574","relatedIdentifierType":"DOI"},{"relationType":"IsDerivedFrom","relatedIdentifier":"10.5281/zenodo.16928877","relatedIdentifierType":"DOI"},{"relationType":"IsCitedBy","relatedIdentifier":"10.1073/pnas.2523462123","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["5613800891 bytes"],"formats":[],"version":"5","rightsList":[{"rights":"Creative Commons Zero v1.0 Universal","rightsUri":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc0-1.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"The liquid-liquid phase separation of biomolecules is an important process\n for intracellular organization. Biomolecular sequence combinatorics leads\n to a large variety of proteins and nucleic acids which can interact to\n form a diversity of dense liquid ('condensate') phases. The\n relationship between sequence design and the diversity of the resultant\n phases is therefore of interest. Here, we explore this question using the\n DNA nanostar system, which permits creation of multi-phase condensate\n droplets through sequence engineering of the sticky end bonds that drive\n particle-particle attraction. We explore the theoretical limits of\n nanostar phase diversity, then experimentally demonstrate the ability to\n create 9 distinct, non-adhering nanostar phases that do not share\n components. We further study how thermal processing affects the morphology\n and dynamics of such a highly diverse condensate system. We particularly\n show that a rapid temperature quench leads to the formation of a densely\n packed 2-D layer of droplets that is transiently stabilized by caging\n effects enabled by the phase diversity, leading to glassy dynamics, such\n as slow coarsening and dynamic heterogeneity. Generally, our work provides\n experimental insight into the thermodynamics of phase separation of\n complex mixtures, and demonstrates the rational engineering of complex,\n long-range, multi-phase droplet structures.","descriptionType":"Abstract"},{"description":"# Data from: Diverse, distinct, and densely packed DNA nanostar droplets\n Dataset DOI: [10.5061/dryad.7d7wm387m](10.5061/dryad.7d7wm387m) ##\n Description of the data and file structure This document describes the\n data repository associated with the paper Diverse, Distinct, and Densely\n Packed DNA Nanostar Droplets by Aria S. Chaderjian, Sam Wilken, and Omar\n A. Saleh. ### Files and variables This repository consists of a .zip\n archive containing 51 data files, as well as a Mathematica notebook and\n its corresponding PDF. The .zip (\n 2025-01-29-DiverseNanostars-DataArchive.zip) contains the following files:\n -This ReadMe file -Three files containing all 4-, 6-, and 8-base DNA\n palindromes with Delta G values calculated from the 2004 SantaLucia\n parameters (see the Mathematica notebook\n GraphStructureOfOrthogonalPalindromes-ForRelease.nb). These files underlie\n Fig. 1D of the main text.      Filenames: *\n AllFourBasePalindromesWithDG.csv * AllSixBasePalindromesWithDG.csv *\n AllEightBasePalindromesWithDG.csv -Three files containing all cliques of\n the maximum size of 3-base orthogonal sticky ends with Delta G within 0.4,\n 0.8, and 1.2 kcal/mol of each other. The data is a list of lists of lists,\n namely a list containing all cliques, with each clique containing lists of\n sticky end sequence and corresponding Delta G value. Cliques contain 8, 9,\n and 10 sticky ends for deltaG spread of 0.4, 0.8, and 1.2 kcal/mol\n respectively. These files were generated from the Mathematica notebook\n GraphStructureOfOrthogonalPalindromes-ForRelease.nb.      Filenames: *\n Cliques04 * Cliques08 * Cliques12 -One file\n 'DimerConcentrations.csv' containing the concentration of each\n complex that resulted from inputting all sticky end sequences with the\n unpaired T as listed in Fig. 1E of the main text, referred to as strands\n 1-9. Each sticky end was input at 12 uM each. This file underlies Fig. 1F\n of the main text. -One file 'All9.ome.tif' which corresponds to\n Fig. 3B-D of the main text. 1 pixel = 0.4887 um. 200 ms exposure time was\n used with 100% LED power. The images are in the order Cy3, Cy5, Atto488.\n No background corrections have been performed (though they were for the\n analysis). -Nine folders 'SingleDark_x.tif' which correspond to\n the single-dark confocal experiments where one NS phase was left unlabeled\n (only visible in brightfield). x in the naming scheme corresponds to the\n NS left unlabeled. Each folder contains 4 files, c0.tif, c1.tif, c2.tif,\n c3.tif, which correspond to Cy3, Atto488, Cy5, and brightfield\n respectively. 1 pixel = 0.22 um. 800 ms exposure time was used for Cy3 and\n Cy5 and 4 s exposure time for Atto488, all with 100% laser power. No\n background corrections have been performed (though they were for the\n analysis). -One video 'PostRamp.ome.tif' which corresponds to\n Fig. 4A of the main text. 1 pixel = 0.733 um. 200 ms exposure time was\n used with 10% LED power. The video begins 40 minutes after the system\n reached 25C with 10 minute intervals. The images are in order (t1, c1),\n (t1, c2), (t1, c3), (t2, c1), (t2, c2), ... where t1 corresponds to the\n first timepoint, c1 corresponds to the Cy3 image, c2 corresponds to the\n Cy5 image, and c3 corresponds to the Atto488 image. No background\n corrections have been performed (though they were for the analysis). -Six\n videos titled 'PostQuench_x.ome.tif' where x corresponds to the\n order in which the video was acquired. All 6 videos are of the same region\n and correspond to Fig. 4B and Fig. 5 of the main text. This was the same\n sample as used in PostRamp.ome.tif, and the same imaging conditions apply\n except that an additional 1.5x magnification setting was turned on, so 1\n pixel = 0.4887 um. No background corrections have been performed. Start\n times (time after reaching 25C) and intervals between frames in the videos\n are as follows, all listed in minutes: ``` x=1    1    5 x=2    260    10\n x=3    1190    10 x=4    2530    10 x=5    3960    20 x=6    7450    10\n ``` ## Code/software A Mathematica notebook titled\n 'GraphStructureOfOrthogonalPalindromes-ForRelease.nb' is\n included, saved also as a PDF. This notebook accomplishes the\n graph-theoretic analysis of the set of all 4-, 6-, and 8-base palindromes,\n outputting lists of the largest groups of mutually orthogonal palindromes\n that accomplish a given spread in binding energy, Delta G. More details\n are included in the comments of the notebook.","descriptionType":"TechnicalInfo"}],"geoLocations":[],"fundingReferences":[{"schemeUri":"https://ror.org","funderName":"W. M. Keck Foundation","funderIdentifier":"https://ror.org/000dswa46","funderIdentifierType":"ROR"},{"schemeUri":"https://ror.org","funderName":"Materials Research Science and Engineering Centers","awardNumber":"DMR 2308708","funderIdentifier":"https://ror.org/01qa5ep47","funderIdentifierType":"ROR"}],"xml":"<?xml version="1.0" encoding="UTF-8"?>
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  <identifier identifierType="DOI">10.5061/DRYAD.7D7WM387M</identifier>
  <creators>
    <creator>
      <creatorName nameType="Personal">Chaderjian, Aria S.</creatorName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0009-0004-8789-0700</nameIdentifier>
      <affiliation affiliationIdentifier="https://ror.org/02t274463" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of California, Santa Barbara</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Saleh, Omar A.</creatorName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0002-9197-4024</nameIdentifier>
      <affiliation affiliationIdentifier="https://ror.org/02t274463" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of California, Santa Barbara</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Data from: Diverse, distinct, and densely packed DNA nanostar droplets</title>
  </titles>
  <publisher publisherIdentifier="https://ror.org/00x6h5n95" publisherIdentifierScheme="ROR" schemeURI="https://ror.org/">Dryad</publisher>
  <resourceType resourceTypeGeneral="Dataset">dataset</resourceType>
  <publicationYear>2026</publicationYear>
  <subjects>
    <subject subjectScheme="fos" schemeURI="https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf">FOS: Physical sciences</subject>
    <subject subjectScheme="fos" schemeURI="https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf">FOS: Materials engineering</subject>
    <subject subjectScheme="fos" schemeURI="https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf">FOS: Chemical engineering</subject>
    <subject subjectScheme="fos" schemeURI="https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf">FOS: Nano-technology</subject>
    <subject>DNA self-assembly</subject>
    <subject>biomolecular liquid</subject>
    <subject>condensate</subject>
    <subject>jammed system</subject>
    <subject>glassy dynamics</subject>
    <subject>Liquid-liquid phase separation</subject>
  </subjects>
  <fundingReferences>
    <fundingReference>
      <funderName>W. M. Keck Foundation</funderName>
      <funderIdentifier funderIdentifierType="ROR">https://ror.org/000dswa46</funderIdentifier>
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  <contributors>
    <contributor contributorType="Sponsor">
      <contributorName>University of California, Santa Barbara</contributorName>
    </contributor>
  </contributors>
  <dates>
    <date dateType="Created">2025-08-22T16:12:27Z</date>
    <date dateType="Submitted">2026-01-30T16:15:14Z</date>
    <date dateType="Issued">2026-02-03T00:00:00Z</date>
    <date dateType="Available">2026-02-03T00:00:00Z</date>
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  <language>en</language>
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  <descriptions>
    <description descriptionType="Abstract">
      The liquid-liquid phase separation of biomolecules is an important process
      for intracellular organization. Biomolecular sequence combinatorics leads
      to a large variety of proteins and nucleic acids which can interact to
      form a diversity of dense liquid ('condensate') phases. The
      relationship between sequence design and the diversity of the resultant
      phases is therefore of interest. Here, we explore this question using the
      DNA nanostar system, which permits creation of multi-phase condensate
      droplets through sequence engineering of the sticky end bonds that drive
      particle-particle attraction. We explore the theoretical limits of
      nanostar phase diversity, then experimentally demonstrate the ability to
      create 9 distinct, non-adhering nanostar phases that do not share
      components. We further study how thermal processing affects the morphology
      and dynamics of such a highly diverse condensate system. We particularly
      show that a rapid temperature quench leads to the formation of a densely
      packed 2-D layer of droplets that is transiently stabilized by caging
      effects enabled by the phase diversity, leading to glassy dynamics, such
      as slow coarsening and dynamic heterogeneity. Generally, our work provides
      experimental insight into the thermodynamics of phase separation of
      complex mixtures, and demonstrates the rational engineering of complex,
      long-range, multi-phase droplet structures.
    </description>
    <description descriptionType="TechnicalInfo">
      # Data from: Diverse, distinct, and densely packed DNA nanostar droplets
      Dataset DOI: [10.5061/dryad.7d7wm387m](10.5061/dryad.7d7wm387m) ##
      Description of the data and file structure This document describes the
      data repository associated with the paper Diverse, Distinct, and Densely
      Packed DNA Nanostar Droplets by Aria S. Chaderjian, Sam Wilken, and Omar
      A. Saleh. ### Files and variables This repository consists of a .zip
      archive containing 51 data files, as well as a Mathematica notebook and
      its corresponding PDF. The .zip (
      2025-01-29-DiverseNanostars-DataArchive.zip) contains the following files:
      -This ReadMe file -Three files containing all 4-, 6-, and 8-base DNA
      palindromes with Delta G values calculated from the 2004 SantaLucia
      parameters (see the Mathematica notebook
      GraphStructureOfOrthogonalPalindromes-ForRelease.nb). These files underlie
      Fig. 1D of the main text.      Filenames: *
      AllFourBasePalindromesWithDG.csv * AllSixBasePalindromesWithDG.csv *
      AllEightBasePalindromesWithDG.csv -Three files containing all cliques of
      the maximum size of 3-base orthogonal sticky ends with Delta G within 0.4,
      0.8, and 1.2 kcal/mol of each other. The data is a list of lists of lists,
      namely a list containing all cliques, with each clique containing lists of
      sticky end sequence and corresponding Delta G value. Cliques contain 8, 9,
      and 10 sticky ends for deltaG spread of 0.4, 0.8, and 1.2 kcal/mol
      respectively. These files were generated from the Mathematica notebook
      GraphStructureOfOrthogonalPalindromes-ForRelease.nb.      Filenames: *
      Cliques04 * Cliques08 * Cliques12 -One file
      'DimerConcentrations.csv' containing the concentration of each
      complex that resulted from inputting all sticky end sequences with the
      unpaired T as listed in Fig. 1E of the main text, referred to as strands
      1-9. Each sticky end was input at 12 uM each. This file underlies Fig. 1F
      of the main text. -One file 'All9.ome.tif' which corresponds to
      Fig. 3B-D of the main text. 1 pixel = 0.4887 um. 200 ms exposure time was
      used with 100% LED power. The images are in the order Cy3, Cy5, Atto488.
      No background corrections have been performed (though they were for the
      analysis). -Nine folders 'SingleDark_x.tif' which correspond to
      the single-dark confocal experiments where one NS phase was left unlabeled
      (only visible in brightfield). x in the naming scheme corresponds to the
      NS left unlabeled. Each folder contains 4 files, c0.tif, c1.tif, c2.tif,
      c3.tif, which correspond to Cy3, Atto488, Cy5, and brightfield
      respectively. 1 pixel = 0.22 um. 800 ms exposure time was used for Cy3 and
      Cy5 and 4 s exposure time for Atto488, all with 100% laser power. No
      background corrections have been performed (though they were for the
      analysis). -One video 'PostRamp.ome.tif' which corresponds to
      Fig. 4A of the main text. 1 pixel = 0.733 um. 200 ms exposure time was
      used with 10% LED power. The video begins 40 minutes after the system
      reached 25C with 10 minute intervals. The images are in order (t1, c1),
      (t1, c2), (t1, c3), (t2, c1), (t2, c2), ... where t1 corresponds to the
      first timepoint, c1 corresponds to the Cy3 image, c2 corresponds to the
      Cy5 image, and c3 corresponds to the Atto488 image. No background
      corrections have been performed (though they were for the analysis). -Six
      videos titled 'PostQuench_x.ome.tif' where x corresponds to the
      order in which the video was acquired. All 6 videos are of the same region
      and correspond to Fig. 4B and Fig. 5 of the main text. This was the same
      sample as used in PostRamp.ome.tif, and the same imaging conditions apply
      except that an additional 1.5x magnification setting was turned on, so 1
      pixel = 0.4887 um. No background corrections have been performed. Start
      times (time after reaching 25C) and intervals between frames in the videos
      are as follows, all listed in minutes: ``` x=1    1    5 x=2    260    10
      x=3    1190    10 x=4    2530    10 x=5    3960    20 x=6    7450    10
      ``` ## Code/software A Mathematica notebook titled
      'GraphStructureOfOrthogonalPalindromes-ForRelease.nb' is
      included, saved also as a PDF. This notebook accomplishes the
      graph-theoretic analysis of the set of all 4-, 6-, and 8-base palindromes,
      outputting lists of the largest groups of mutually orthogonal palindromes
      that accomplish a given spread in binding energy, Delta G. More details
      are included in the comments of the notebook.
    </description>
  </descriptions>
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