{"data":{"id":"10.5061/dryad.5p0c3","type":"dois","attributes":{"doi":"10.5061/dryad.5p0c3","prefix":"10.5061","suffix":"dryad.5p0c3","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Mikheyev, Alexander S.","nameType":"Personal","givenName":"Alexander S.","familyName":"Mikheyev","affiliation":["Okinawa Institute of Science and Technology Graduate University"],"nameIdentifiers":[]},{"name":"Tin, Mandy M.Y.","nameType":"Personal","givenName":"Mandy M.Y.","familyName":"Tin","affiliation":["Okinawa Institute of Science and Technology Graduate University"],"nameIdentifiers":[]},{"name":"Tin, Mandy M. Y.","nameType":"Personal","givenName":"Mandy M. Y.","familyName":"Tin","affiliation":["Okinawa Institute of Science and Technology Graduate University"],"nameIdentifiers":[]}],"titles":[{"title":"Data from: A first look at the Oxford Nanopore MinION sequencer"}],"publisher":"Dryad","container":{},"publicationYear":2014,"subjects":[{"subject":"Genomics/Proteomics"},{"subject":"Next-generation sequencing","schemeUri":"https://github.com/PLOS/plos-thesaurus","subjectScheme":"PLOS Subject Area Thesaurus"},{"subject":"Bioinformatics","schemeUri":"https://github.com/PLOS/plos-thesaurus","subjectScheme":"PLOS Subject Area Thesaurus"},{"subject":"FOS: Computer and information sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Protobothrops flavoviridis"}],"contributors":[],"dates":[{"date":"2014-07-03T13:25:21Z","dateType":"Submitted"},{"date":"2014-09-04T15:42:31Z","dateType":"Issued"},{"date":"2014-09-04T15:42:31Z","dateType":"Available"}],"language":"en","types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsCitedBy","relatedIdentifier":"10.1111/1755-0998.12324","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["2680345451 bytes"],"formats":[],"version":"1","rightsList":[{"rights":"Creative Commons Zero v1.0 Universal","rightsUri":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc0-1.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Oxford Nanopore's third-generation, single-molecule sequencing\n platform promises to decrease costs for reagents and instrumentation.\n After a two-year hiatus following the initial announcement, the first\n devices have been released as part of an early access program. We explore\n the performance of this platform by re-sequencing the lambda phage genome,\n and amplicons from a snake venom gland transcriptome. Although the\n handheld MinION sequencer can generate more than 150 megabases of raw data\n in one run, at most a quarter of the resulting reads map to the reference,\n with less than average 10% identity. Much of the sequence consists of\n insertion/deletion errors, or is seemingly without similarity to the\n template. Using the lambda phage data as an example, although the reads\n are long, averaging 5kb, at best 890±1,932 bases per mapped read could be\n matched to the reference without soft clipping. In the course of a 36 hour\n run on the MinION it was possible to re-sequence the 48kb lambda phage\n reference at 16x coverage. Currently substantially larger projects would\n not be feasible using the MinION. Without increases in accuracy, which\n would be required for applications such as genome scaffolding and phasing,\n the current utility of the MinION appears limited. Library preparation\n requires access to a molecular lab, and is of similar complexity and cost\n to that of other next-generation sequencing platforms. The MinION is an\n exciting step in a new direction for single-molecule sequencing, though it\n will require dramatic decreases in error rates before it lives up to its\n promise.","descriptionType":"Abstract"},{"description":"Raw\n readsLambda phage reads\n processed with the 1D and 2D workflows.reads.tar.gzReads for snake transcriptome dataReads for venom gland transcriptome of Protobothrops flavoviridis.snake.tar.gzprocessed_data.tar.gzThis file contains all the analysis files except raw Illumina data (available as a separate file) and raw Oxford Nanopore read in FAST5 format. As FAST5 files require Oxford Nanopore's proprietary software to analyze and are large (~30G compressed), they are probably not useful to most readers, but are available from the authors upon request.Illumina forward reads for the Protobothrops flavoviridis transcriptomeFrom: Aird, S.D. et al., 2013. Quantitative high-throughput profiling of snake venom gland transcriptomes and proteomes (Ovophis okinavensis and Protobothrops flavoviridis). BMC Genomics, 14(1), p.790.GGCTAC_pe1.fq.gzIllumina reverse reads for the Protobothrops flavoviridis transcriptomeFrom: Aird, S.D. et al., 2013. Quantitative high-throughput profiling of snake venom gland transcriptomes and proteomes (Ovophis okinavensis and Protobothrops flavoviridis). BMC Genomics, 14(1), p.790.GGCTAC_pe2.fq.gz","descriptionType":"Other"}],"geoLocations":[{"geoLocationPlace":"Japan"},{"geoLocationPlace":"Okinawa"}],"fundingReferences":[],"xml":"<?xml version="1.0" encoding="UTF-8"?>
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      <creatorName nameType="Personal">Mikheyev, Alexander S.</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/02qg15b79" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Okinawa Institute of Science and Technology Graduate University</affiliation>
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      <creatorName nameType="Personal">Tin, Mandy M.Y.</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/02qg15b79" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Okinawa Institute of Science and Technology Graduate University</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Tin, Mandy M. Y.</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/02qg15b79" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Okinawa Institute of Science and Technology Graduate University</affiliation>
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  <titles>
    <title>Data from: A first look at the Oxford Nanopore MinION sequencer</title>
  </titles>
  <publisher publisherIdentifier="https://ror.org/00x6h5n95" publisherIdentifierScheme="ROR" schemeURI="https://ror.org/">Dryad</publisher>
  <resourceType resourceTypeGeneral="Dataset">dataset</resourceType>
  <publicationYear>2014</publicationYear>
  <subjects>
    <subject>Genomics/Proteomics</subject>
    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Next-generation sequencing</subject>
    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Bioinformatics</subject>
    <subject>Protobothrops flavoviridis</subject>
  </subjects>
  <dates>
    <date dateType="Submitted">2014-07-03T13:25:21Z</date>
    <date dateType="Issued">2014-09-04T15:42:31Z</date>
    <date dateType="Available">2014-09-04T15:42:31Z</date>
  </dates>
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    <description descriptionType="Abstract">
      Oxford Nanopore's third-generation, single-molecule sequencing
      platform promises to decrease costs for reagents and instrumentation.
      After a two-year hiatus following the initial announcement, the first
      devices have been released as part of an early access program. We explore
      the performance of this platform by re-sequencing the lambda phage genome,
      and amplicons from a snake venom gland transcriptome. Although the
      handheld MinION sequencer can generate more than 150 megabases of raw data
      in one run, at most a quarter of the resulting reads map to the reference,
      with less than average 10% identity. Much of the sequence consists of
      insertion/deletion errors, or is seemingly without similarity to the
      template. Using the lambda phage data as an example, although the reads
      are long, averaging 5kb, at best 890±1,932 bases per mapped read could be
      matched to the reference without soft clipping. In the course of a 36 hour
      run on the MinION it was possible to re-sequence the 48kb lambda phage
      reference at 16x coverage. Currently substantially larger projects would
      not be feasible using the MinION. Without increases in accuracy, which
      would be required for applications such as genome scaffolding and phasing,
      the current utility of the MinION appears limited. Library preparation
      requires access to a molecular lab, and is of similar complexity and cost
      to that of other next-generation sequencing platforms. The MinION is an
      exciting step in a new direction for single-molecule sequencing, though it
      will require dramatic decreases in error rates before it lives up to its
      promise.
    </description>
    <description descriptionType="Other">
      &lt;div class="o-metadata__file-usage-entry"&gt;&lt;h4
      class="o-heading__level3-file-title"&gt;Raw
      reads&lt;/h4&gt;&lt;div
      class="o-metadata__file-description"&gt;Lambda phage reads
      processed with the 1D and 2D workflows.&lt;/div&gt;&lt;div
      class="o-metadata__file-name"&gt;reads.tar.gz&lt;/br&gt;&lt;/div&gt;&lt;/div&gt;&lt;div class="o-metadata__file-usage-entry"&gt;&lt;h4 class="o-heading__level3-file-title"&gt;Reads for snake transcriptome data&lt;/h4&gt;&lt;div class="o-metadata__file-description"&gt;Reads for venom gland transcriptome of Protobothrops flavoviridis.&lt;/div&gt;&lt;div class="o-metadata__file-name"&gt;snake.tar.gz&lt;/br&gt;&lt;/div&gt;&lt;/div&gt;&lt;div class="o-metadata__file-usage-entry"&gt;&lt;h4 class="o-heading__level3-file-title"&gt;processed_data.tar.gz&lt;/h4&gt;&lt;div class="o-metadata__file-description"&gt;This file contains all the analysis files except raw Illumina data (available as a separate file) and raw Oxford Nanopore read in FAST5 format. As FAST5 files require Oxford Nanopore's proprietary software to analyze and are large (~30G compressed), they are probably not useful to most readers, but are available from the authors upon request.&lt;/div&gt;&lt;div class="o-metadata__file-name"&gt;&lt;/div&gt;&lt;/div&gt;&lt;div class="o-metadata__file-usage-entry"&gt;&lt;h4 class="o-heading__level3-file-title"&gt;Illumina forward reads for the Protobothrops flavoviridis transcriptome&lt;/h4&gt;&lt;div class="o-metadata__file-description"&gt;From: Aird, S.D. et al., 2013. Quantitative high-throughput profiling of snake venom gland transcriptomes and proteomes (Ovophis okinavensis and Protobothrops flavoviridis). BMC Genomics, 14(1), p.790.&lt;/div&gt;&lt;div class="o-metadata__file-name"&gt;GGCTAC_pe1.fq.gz&lt;/br&gt;&lt;/div&gt;&lt;/div&gt;&lt;div class="o-metadata__file-usage-entry"&gt;&lt;h4 class="o-heading__level3-file-title"&gt;Illumina reverse reads for the Protobothrops flavoviridis transcriptome&lt;/h4&gt;&lt;div class="o-metadata__file-description"&gt;From: Aird, S.D. et al., 2013. Quantitative high-throughput profiling of snake venom gland transcriptomes and proteomes (Ovophis okinavensis and Protobothrops flavoviridis). BMC Genomics, 14(1), p.790.&lt;/div&gt;&lt;div class="o-metadata__file-name"&gt;GGCTAC_pe2.fq.gz&lt;/br&gt;&lt;/div&gt;&lt;/div&gt;
    </description>
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