{"data":{"id":"10.5061/dryad.51c59zwb7","type":"dois","attributes":{"doi":"10.5061/dryad.51c59zwb7","prefix":"10.5061","suffix":"dryad.51c59zwb7","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Jiang, Fan","nameType":"Personal","givenName":"Fan","familyName":"Jiang","affiliation":["Shandong University"],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0000-0001-9466-2192","nameIdentifierScheme":"ORCID"}]}],"titles":[{"title":"Genome wide mRNA sequencing data in macrophages without and with CX-5461 treatment"}],"publisher":"Dryad","container":{},"publicationYear":2022,"subjects":[{"subject":"FOS: Basic medicine","schemeUri":"https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf","subjectScheme":"fos"},{"subject":"FOS: Basic medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2022-05-17T07:53:05Z","dateType":"Submitted"},{"date":"2022-05-23T00:00:00Z","dateType":"Issued"},{"date":"2022-05-23T00:00:00Z","dateType":"Available"}],"language":"en","types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsCitedBy","relatedIdentifier":"10.3389/fphar.2022.926317","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["16426876 bytes"],"formats":[],"version":"2","rightsList":[{"rights":"Creative Commons Zero v1.0 Universal","rightsUri":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc0-1.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"CX-5461, a novel selective RNA polymerase I inhibitor, shows potential\n anti-inflammatory and immunosuppressive activities. However, the molecular\n mechanisms underlying the inhibitory effects of CX-5461 on\n macrophage-mediated inflammation remain to be clarified. In the present\n study, we attempted to identify the systemic biological processes which\n were modulated by CX-5461 in inflammatory macrophages. Primary peritoneal\n macrophages were isolated from normal Sprague Dawley rats, and primed with\n lipopolysaccharide or interferon-gamma. Genome-wide RNA sequencing was\n performed. The study suggests that limiting cell proliferation\n predominates in the inhibitory effects of CX-5461 on macrophage-mediated\n inflammation. ","descriptionType":"Abstract"},{"description":"The RNA processing and\n sequencing services were provided by LC-Bio Technology (Hangzhou, Zhejiang\n Province, China). Total RNA was extracted using Trizol reagent (Thermo\n Fisher) following the manufacturer's instructions. The RNA quantity\n and purity were analyzed using Bioanalyzer 2100 and RNA 6000 Nano LabChip\n Kit (from Agilent, Santa Clara, CA, USA). RNA samples with RIN number\n \u0026gt; 7.0 were used for library construction. Purified mRNA was\n obtained from 5 mg of total RNA using Dynabeads Oligo (dT)\n (Thermo Fisher) with two rounds of purification; the mRNA was then\n fragmented using divalent cations (Magnesium RNA Fragmentation Module from\n New England Biolabs, Ipswich, MA, USA) under 94oC for 5 min. The cleaved\n mRNAs were reverse-transcribed to cDNAs using SuperScript II Reverse\n Transcriptase (Thermo Fisher), which were used as templates to synthesize\n U-labeled second-strand DNAs with\u003cem\u003e E. coli\u003c/em\u003e DNA\n polymerase I, RNase H (all from New England Biolabs) and dUTP (Thermo\n Fisher). After ligation with Dual-index adapters, the DNA library was\n treated with heat-labile UDG enzyme (New England Biolabs) to remove the\n U-labeled second strands, and amplified by PCR. The average insert size of\n the final cDNA library was 300 ± 50 bp. Paired-end sequencing (2 × 150 bp)\n was performed using a NovaSeq 6000 Sequencing System (Illumina, San Diego,\n CA, USA). High quality clean reads were obtained by filtering with\n Cutadapt (version 1.9) (https://cutadapt.readthedocs.io/en/stable/) and\n verified with FastQC\n (http://www.bioinformatics.babraham.ac.uk/projects/fastqc). Read alignment\n to the Rattus norvegicus reference genome was performed using HISAT2\n package (version 2.0.4) (https://daehwankimlab.github.io/hisat2). Read\n assembly was performed using StringTie (version 1.3.4d)\n (http://ccb.jhu.edu/software/stringtie). StringTie and Ballgown\n (http://www.bioconductor.org/packages/release/bioc/html/ballgown.html)\n were used to estimate the relative expression levels (expressed as\n fragment per kilobase per million reads/FPKM values).","descriptionType":"Methods"},{"description":"In each experimental group, 3\n independent samples were included. \n Sample identity:\n control1-control3: Control\n group IFN1-IFN3: Interferon-gamma-treated group\n IFN_CX1- IFN_CX3: Interferon-gamma + CX-5461\n co-treated group LPS1-LPS3: Lipopolysaccharide-treated\n group LPS_CX1- LPS_CX3: Lipopolysaccharide + CX-5461\n co-treated group Concentrations used:\n CX-5461 (1 uM) IFN-gamma (150 U/mL)\n LPS (1 ug/mL)","descriptionType":"Other"}],"geoLocations":[],"fundingReferences":[{"schemeUri":"https://ror.org","funderName":"National Natural Science Foundation of China","awardNumber":"82070265","funderIdentifier":"https://ror.org/01h0zpd94","funderIdentifierType":"ROR"}],"xml":"<?xml version="1.0" encoding="UTF-8"?>
<resource xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns="http://datacite.org/schema/kernel-4" xsi:schemaLocation="http://datacite.org/schema/kernel-4 http://schema.datacite.org/meta/kernel-4/metadata.xsd">
  <identifier identifierType="DOI">10.5061/DRYAD.51C59ZWB7</identifier>
  <creators>
    <creator>
      <creatorName nameType="Personal">Jiang, Fan</creatorName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0001-9466-2192</nameIdentifier>
      <affiliation affiliationIdentifier="https://ror.org/0207yh398" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Shandong University</affiliation>
    </creator>
  </creators>
  <titles>
    <title>
      Genome wide mRNA sequencing data in macrophages without and with CX-5461
      treatment
    </title>
  </titles>
  <publisher publisherIdentifier="https://ror.org/00x6h5n95" publisherIdentifierScheme="ROR" schemeURI="https://ror.org/">Dryad</publisher>
  <resourceType resourceTypeGeneral="Dataset">dataset</resourceType>
  <publicationYear>2022</publicationYear>
  <subjects>
    <subject subjectScheme="fos" schemeURI="https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf">FOS: Basic medicine</subject>
  </subjects>
  <fundingReferences>
    <fundingReference>
      <funderName>National Natural Science Foundation of China</funderName>
      <funderIdentifier funderIdentifierType="ROR">https://ror.org/01h0zpd94</funderIdentifier>
      <awardNumber>82070265</awardNumber>
    </fundingReference>
  </fundingReferences>
  <dates>
    <date dateType="Submitted">2022-05-17T07:53:05Z</date>
    <date dateType="Issued">2022-05-23T00:00:00Z</date>
    <date dateType="Available">2022-05-23T00:00:00Z</date>
  </dates>
  <language>en</language>
  <relatedIdentifiers>
    <relatedIdentifier relationType="IsCitedBy" relatedIdentifierType="DOI">https://doi.org/10.3389/fphar.2022.926317</relatedIdentifier>
  </relatedIdentifiers>
  <sizes>
    <size>16426876 bytes</size>
  </sizes>
  <version>2</version>
  <rightsList>
    <rights rightsURI="https://spdx.org/licenses/CC0-1.0.html">Creative Commons Zero v1.0 Universal</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">
      CX-5461, a novel selective RNA polymerase I inhibitor, shows potential
      anti-inflammatory and immunosuppressive activities. However, the molecular
      mechanisms underlying the inhibitory effects of CX-5461 on
      macrophage-mediated inflammation remain to be clarified. In the present
      study, we attempted to identify the systemic biological processes which
      were modulated by CX-5461 in inflammatory macrophages. Primary peritoneal
      macrophages were isolated from normal Sprague Dawley rats, and primed with
      lipopolysaccharide or interferon-gamma. Genome-wide RNA sequencing was
      performed. The study suggests that limiting cell proliferation
      predominates in the inhibitory effects of CX-5461 on macrophage-mediated
      inflammation.&amp;nbsp;
    </description>
    <description descriptionType="Methods">
      &lt;p&gt;&lt;span lang="EN-US"&gt;The RNA processing and
      sequencing services were provided by LC-Bio Technology (Hangzhou, Zhejiang
      Province, China). Total RNA was extracted using Trizol reagent (Thermo
      Fisher) following the manufacturer's instructions. The RNA quantity
      and purity were analyzed using Bioanalyzer 2100 and RNA 6000 Nano LabChip
      Kit (from Agilent, Santa Clara, CA, USA). RNA samples with RIN number
      &amp;gt; 7.0 were used for library construction. Purified mRNA was
      obtained from 5 &lt;/span&gt;&lt;span
      lang="EN-US"&gt;m&lt;/span&gt;&lt;span
      lang="EN-US"&gt;g of total RNA using Dynabeads Oligo (dT)
      (Thermo Fisher) with two rounds of purification; the mRNA was then
      fragmented using divalent cations (Magnesium RNA Fragmentation Module from
      New England Biolabs, Ipswich, MA, USA) under 94oC for 5 min. The cleaved
      mRNAs were reverse-transcribed to cDNAs using SuperScript II Reverse
      Transcriptase (Thermo Fisher), which were used as templates to synthesize
      U-labeled second-strand DNAs with&lt;em&gt; E. coli&lt;/em&gt; DNA
      polymerase I, RNase H (all from New England Biolabs) and dUTP (Thermo
      Fisher). After ligation with Dual-index adapters, the DNA library was
      treated with heat-labile UDG enzyme (New England Biolabs) to remove the
      U-labeled second strands, and amplified by PCR. The average insert size of
      the final cDNA library was 300 ± 50 bp. Paired-end sequencing (2 × 150 bp)
      was performed using a NovaSeq 6000 Sequencing System (Illumina, San Diego,
      CA, USA). High quality clean reads were obtained by filtering with
      Cutadapt (version 1.9) (https://cutadapt.readthedocs.io/en/stable/) and
      verified with FastQC
      (http://www.bioinformatics.babraham.ac.uk/projects/fastqc). Read alignment
      to the Rattus norvegicus reference genome was performed using HISAT2
      package (version 2.0.4) (https://daehwankimlab.github.io/hisat2). Read
      assembly was performed using StringTie (version 1.3.4d)
      (http://ccb.jhu.edu/software/stringtie). StringTie and Ballgown
      (http://www.bioconductor.org/packages/release/bioc/html/ballgown.html)
      were used to estimate the relative expression levels (expressed as
      fragment per kilobase per million reads/FPKM values).
      &lt;/span&gt;&lt;/p&gt;
    </description>
    <description descriptionType="Other">
      &lt;p&gt;&lt;span lang="EN-US"&gt;In each experimental group, 3
      independent samples were included. &lt;/span&gt;&lt;/p&gt;
      &lt;p&gt;&lt;span lang="EN-US"&gt;Sample identity:
      &lt;/span&gt;&lt;/p&gt; &lt;p class="MsoNormal"&gt;&lt;span
      lang="EN-US"&gt;control1-control3: Control
      group&lt;/span&gt;&lt;/p&gt; &lt;p class="MsoNormal"&gt;&lt;span
      lang="EN-US"&gt;IFN1-IFN3: Interferon-gamma-treated group
      &lt;/span&gt;&lt;/p&gt; &lt;p class="MsoNormal"&gt;&lt;span
      lang="EN-US"&gt;IFN_CX1- IFN_CX3: Interferon-gamma + CX-5461
      co-treated group&lt;/span&gt;&lt;/p&gt; &lt;p
      class="MsoNormal"&gt;&lt;span
      lang="EN-US"&gt;LPS1-LPS3: Lipopolysaccharide-treated
      group&lt;/span&gt;&lt;/p&gt; &lt;p class="MsoNormal"&gt;&lt;span
      lang="EN-US"&gt;LPS_CX1- LPS_CX3: Lipopolysaccharide + CX-5461
      co-treated group&lt;/span&gt;&lt;/p&gt; &lt;p
      class="MsoNormal"&gt;&lt;span
      lang="EN-US"&gt;Concentrations used:&lt;/span&gt;&lt;/p&gt;
      &lt;p class="MsoNormal"&gt;&lt;span
      lang="EN-US"&gt;CX-5461 (1 uM)&lt;/span&gt;&lt;/p&gt; &lt;p
      class="MsoNormal"&gt;&lt;span
      lang="EN-US"&gt;IFN-gamma (150 U/mL)&lt;/span&gt;&lt;/p&gt;
      &lt;p class="MsoNormal"&gt;&lt;span
      lang="EN-US"&gt;LPS (1 ug/mL) &lt;/span&gt;&lt;/p&gt;
    </description>
  </descriptions>
</resource>","url":"https://datadryad.org/dataset/doi:10.5061/dryad.51c59zwb7","contentUrl":null,"metadataVersion":9,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":336,"viewsOverTime":[{"yearMonth":"2022-05","total":3},{"yearMonth":"2022-06","total":3},{"yearMonth":"2022-07","total":1},{"yearMonth":"2022-09","total":3},{"yearMonth":"2022-10","total":12},{"yearMonth":"2022-11","total":7},{"yearMonth":"2022-12","total":7},{"yearMonth":"2023-01","total":4},{"yearMonth":"2023-02","total":7},{"yearMonth":"2023-03","total":6},{"yearMonth":"2023-04","total":7},{"yearMonth":"2023-05","total":10},{"yearMonth":"2023-06","total":10},{"yearMonth":"2023-07","total":6},{"yearMonth":"2023-08","total":26},{"yearMonth":"2023-09","total":29},{"yearMonth":"2023-10","total":7},{"yearMonth":"2023-11","total":11},{"yearMonth":"2023-12","total":3},{"yearMonth":"2024-01","total":4},{"yearMonth":"2024-03","total":1},{"yearMonth":"2024-04","total":15},{"yearMonth":"2024-05","total":30},{"yearMonth":"2024-06","total":18},{"yearMonth":"2024-07","total":20},{"yearMonth":"2024-08","total":23},{"yearMonth":"2024-09","total":23},{"yearMonth":"2024-10","total":5},{"yearMonth":"2024-11","total":3},{"yearMonth":"2024-12","total":4},{"yearMonth":"2025-01","total":2},{"yearMonth":"2025-02","total":1},{"yearMonth":"2025-07","total":2},{"yearMonth":"2025-09","total":2},{"yearMonth":"2025-10","total":2},{"yearMonth":"2025-11","total":1},{"yearMonth":"2026-02","total":2},{"yearMonth":"2026-03","total":2},{"yearMonth":"2026-04","total":1},{"yearMonth":"2026-05","total":3},{"yearMonth":"2026-06","total":2},{"yearMonth":"2026-07","total":3},{"yearMonth":"2026-08","total":1},{"yearMonth":"2026-09","total":4}],"downloadCount":11,"downloadsOverTime":[{"yearMonth":"2022-06","total":1},{"yearMonth":"2022-10","total":2},{"yearMonth":"2022-12","total":1},{"yearMonth":"2023-03","total":1},{"yearMonth":"2023-04","total":1},{"yearMonth":"2023-11","total":1},{"yearMonth":"2023-12","total":1},{"yearMonth":"2024-01","total":0},{"yearMonth":"2024-03","total":0},{"yearMonth":"2024-04","total":0},{"yearMonth":"2024-05","total":0},{"yearMonth":"2024-06","total":0},{"yearMonth":"2024-07","total":0},{"yearMonth":"2024-08","total":0},{"yearMonth":"2024-09","total":1},{"yearMonth":"2024-10","total":0},{"yearMonth":"2024-11","total":0},{"yearMonth":"2024-12","total":1},{"yearMonth":"2025-01","total":0},{"yearMonth":"2025-02","total":1},{"yearMonth":"2025-07","total":0},{"yearMonth":"2025-09","total":0},{"yearMonth":"2025-10","total":0},{"yearMonth":"2025-11","total":0},{"yearMonth":"2026-02","total":0},{"yearMonth":"2026-03","total":0},{"yearMonth":"2026-04","total":0},{"yearMonth":"2026-05","total":0},{"yearMonth":"2026-06","total":0},{"yearMonth":"2026-07","total":0},{"yearMonth":"2026-08","total":0},{"yearMonth":"2026-09","total":0}],"referenceCount":0,"citationCount":1,"citationsOverTime":[{"year":"2026","total":1}],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2022-05-23T17:52:30.000Z","registered":"2022-05-23T17:52:31.000Z","published":"2022","updated":"2026-05-07T17:22:22.000Z"},"relationships":{"client":{"data":{"id":"dryad.dryad","type":"clients"}},"provider":{"data":{"id":"dryad","type":"providers"}},"media":{"data":{"id":"10.5061/dryad.51c59zwb7","type":"media"}},"references":{"data":[]},"citations":{"data":[{"id":"10.3389/fphar.2022.926317","type":"dois"}]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}