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We sought to determine whether, like\n tapasin, TAPBPR can also influence MHC I peptide selection by functioning\n as a peptide exchange catalyst. We show that TAPBPR can catalyse the\n dissociation of peptides from peptide-MHC I complexes, enhance the loading\n of peptide-receptive MHC I molecules, and discriminate between peptides\n based on affinity in vitro. In cells, the depletion of TAPBPR increased\n the diversity of peptides presented on MHC I molecules, suggesting that\n TAPBPR is involved in restricting peptide presentation. Our results\n suggest TAPBPR binds to MHC I in a peptide-receptive state and, like\n tapasin, works to enhance peptide optimisation. It is now clear there are\n two MHC class I specific peptide editors, tapasin and TAPBPR, intimately\n involved in controlling peptide presentation to the immune system.","descriptionType":"Abstract"},{"description":"Figure 5C - Peptides\n eluted from MHC class I molecules in Hela+IFNy and Hela-TAPBPR\n KO+IFNyPeptide-MHC class I\n complexes were isolated by affinity chromatography using W6/32 from IFN-γ\n treated HeLa and HeLa-TAPBPR KO cells. Eluted peptides were analysed using\n LC-MS/MS. Peptides were assigned as HLA-A*68:02 and HLA-B*15:03 binders\n based on their peptide motifs using the online programme\n NetMHC.Hela\n IFNy and Hela IFNy TAPBPR\n KO.xlsxFigure 5D - peptides\n eluted from HeLa and HeLa-WT-TAPBPR cellsPeptide-MHC class I\n complexes were isolated by affinity chromatography using W6/32 from HeLa\n and HeLa overexpressing WT-TAPBPR. Eluted peptides were analysed using\n LC-MS/MS. Peptides were assigned as HLA-A*68:02 and HLA-B*15:03 binders\n based on their peptide motifs using the online programme\n NetMHC.HeLa\n and HeLa WT TAPBPR.xlsxFigure 5 - Figure\n supplement 1 - Peptides eluted from HeLa-S+IFNy and\n HeLa-S-shTAPBPR+IFNyPeptide-MHC class I\n complexes were isolated by affinity chromatography using W6/32 from IFN-γ\n treated HeLa-S cells and IFN-γ treated HeLa-S cells depleted of TAPBPR\n using shRNA. Eluted peptides were analysed using LC-MS/MS. Peptides were\n assigned as HLA-A*68:02 and HLA-B*15:03 binders based on their peptide\n motifs using the online programme NetMHC.HeLa IFNy and HeLa INFy\n shTAPBPR.xlsxFigure 6B - Peptide\n eluted from HLA-A2 from WT, TAPBPR depleted, and tapasin deficient KBM-7\n cellsPeptide loaded HLA-A2\n complexes were isolated using affinity chromatography with BB7.2 from\n IFN-γ treated WT, TAPBPR stably depleted (shTAPBPR) and tapasin deficient\n (TPN-) KBM-7 cells. Eluted peptide were analysed using\n LC-MS/MS.KBM7 elution from\n BB72.xlsxFigure 6E - Peptides\n eluted from HLA-B \u0026amp; -C from WT, TAPBPR depleted and tapasin deficient\n KBM-7 cellsHLA-B and -C complexes\n were isolated using affinity chromatography using B1.23.2 from IFN-γ\n treated WT, TAPBPR stably depleted (shTAPBPR) and tapasin deficient (TPN-)\n KBM-7 cells. Eluted peptide were analysed using\n LC-MS/MS.KBM7 elution from\n B1232.xlsx","descriptionType":"Other"}],"geoLocations":[],"fundingReferences":[],"xml":"<?xml version="1.0" encoding="UTF-8"?>
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  <identifier identifierType="DOI">10.5061/DRYAD.487J9</identifier>
  <creators>
    <creator>
      <creatorName nameType="Personal">Hermann, Clemens</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/013meh722" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of Cambridge</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">van Hateren, Andy</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/01ryk1543" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of Southampton</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Trautwein, Nico</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/01ryk1543" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of Southampton</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Neerincx, Andreas</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/013meh722" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of Cambridge</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Duriez, Patrick J.</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/01ryk1543" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of Southampton</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Stevanović, Stefan</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/03a1kwz48" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of Tübingen</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Trowsdale, John</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/013meh722" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of Cambridge</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Deane, Janet E.</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/013meh722" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of Cambridge</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Elliott, Tim</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/01ryk1543" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of Southampton</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Boyle, Louise H.</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/013meh722" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of Cambridge</affiliation>
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  <titles>
    <title>
      Data from: TAPBPR alters MHC class I peptide presentation by functioning
      as a peptide exchange catalyst
    </title>
  </titles>
  <publisher publisherIdentifier="https://ror.org/00x6h5n95" publisherIdentifierScheme="ROR" schemeURI="https://ror.org/">Dryad</publisher>
  <resourceType resourceTypeGeneral="Dataset">dataset</resourceType>
  <publicationYear>2015</publicationYear>
  <subjects>
    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Cell biology</subject>
    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Antigen presentation</subject>
    <subject>MHC</subject>
    <subject>antigen processing</subject>
    <subject>HLA</subject>
    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Immunology</subject>
  </subjects>
  <dates>
    <date dateType="Submitted">2015-10-08T11:58:15Z</date>
    <date dateType="Issued">2015-10-08T17:36:24Z</date>
    <date dateType="Available">2015-10-08T17:36:24Z</date>
  </dates>
  <language>en</language>
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  <descriptions>
    <description descriptionType="Abstract">
      Our understanding of the antigen presentation pathway has recently been
      enhanced with the identification that the tapasin-related protein TAPBPR
      is a second MHC I-specific chaperone. We sought to determine whether, like
      tapasin, TAPBPR can also influence MHC I peptide selection by functioning
      as a peptide exchange catalyst. We show that TAPBPR can catalyse the
      dissociation of peptides from peptide-MHC I complexes, enhance the loading
      of peptide-receptive MHC I molecules, and discriminate between peptides
      based on affinity in vitro. In cells, the depletion of TAPBPR increased
      the diversity of peptides presented on MHC I molecules, suggesting that
      TAPBPR is involved in restricting peptide presentation. Our results
      suggest TAPBPR binds to MHC I in a peptide-receptive state and, like
      tapasin, works to enhance peptide optimisation. It is now clear there are
      two MHC class I specific peptide editors, tapasin and TAPBPR, intimately
      involved in controlling peptide presentation to the immune system.
    </description>
    <description descriptionType="Other">
      &lt;div class="o-metadata__file-usage-entry"&gt;&lt;h4
      class="o-heading__level3-file-title"&gt;Figure 5C - Peptides
      eluted from MHC class I molecules in Hela+IFNy and Hela-TAPBPR
      KO+IFNy&lt;/h4&gt;&lt;div
      class="o-metadata__file-description"&gt;Peptide-MHC class I
      complexes were isolated by affinity chromatography using W6/32 from IFN-γ
      treated HeLa and HeLa-TAPBPR KO cells. Eluted peptides were analysed using
      LC-MS/MS. Peptides were assigned as HLA-A*68:02 and HLA-B*15:03 binders
      based on their peptide motifs using the online programme
      NetMHC.&lt;/div&gt;&lt;div class="o-metadata__file-name"&gt;Hela
      IFNy and Hela IFNy TAPBPR
      KO.xlsx&lt;/br&gt;&lt;/div&gt;&lt;/div&gt;&lt;div
      class="o-metadata__file-usage-entry"&gt;&lt;h4
      class="o-heading__level3-file-title"&gt;Figure 5D - peptides
      eluted from HeLa and HeLa-WT-TAPBPR cells&lt;/h4&gt;&lt;div
      class="o-metadata__file-description"&gt;Peptide-MHC class I
      complexes were isolated by affinity chromatography using W6/32 from HeLa
      and HeLa overexpressing WT-TAPBPR. Eluted peptides were analysed using
      LC-MS/MS. Peptides were assigned as HLA-A*68:02 and HLA-B*15:03 binders
      based on their peptide motifs using the online programme
      NetMHC.&lt;/div&gt;&lt;div class="o-metadata__file-name"&gt;HeLa
      and HeLa WT TAPBPR.xlsx&lt;/br&gt;&lt;/div&gt;&lt;/div&gt;&lt;div
      class="o-metadata__file-usage-entry"&gt;&lt;h4
      class="o-heading__level3-file-title"&gt;Figure 5 - Figure
      supplement 1 - Peptides eluted from HeLa-S+IFNy and
      HeLa-S-shTAPBPR+IFNy&lt;/h4&gt;&lt;div
      class="o-metadata__file-description"&gt;Peptide-MHC class I
      complexes were isolated by affinity chromatography using W6/32 from IFN-γ
      treated HeLa-S cells and IFN-γ treated HeLa-S cells depleted of TAPBPR
      using shRNA. Eluted peptides were analysed using LC-MS/MS. Peptides were
      assigned as HLA-A*68:02 and HLA-B*15:03 binders based on their peptide
      motifs using the online programme NetMHC.&lt;/div&gt;&lt;div
      class="o-metadata__file-name"&gt;HeLa IFNy and HeLa INFy
      shTAPBPR.xlsx&lt;/br&gt;&lt;/div&gt;&lt;/div&gt;&lt;div
      class="o-metadata__file-usage-entry"&gt;&lt;h4
      class="o-heading__level3-file-title"&gt;Figure 6B - Peptide
      eluted from HLA-A2 from WT, TAPBPR depleted, and tapasin deficient KBM-7
      cells&lt;/h4&gt;&lt;div
      class="o-metadata__file-description"&gt;Peptide loaded HLA-A2
      complexes were isolated using affinity chromatography with BB7.2 from
      IFN-γ treated WT, TAPBPR stably depleted (shTAPBPR) and tapasin deficient
      (TPN-) KBM-7 cells. Eluted peptide were analysed using
      LC-MS/MS.&lt;/div&gt;&lt;div
      class="o-metadata__file-name"&gt;KBM7 elution from
      BB72.xlsx&lt;/br&gt;&lt;/div&gt;&lt;/div&gt;&lt;div
      class="o-metadata__file-usage-entry"&gt;&lt;h4
      class="o-heading__level3-file-title"&gt;Figure 6E - Peptides
      eluted from HLA-B &amp; -C from WT, TAPBPR depleted and tapasin deficient
      KBM-7 cells&lt;/h4&gt;&lt;div
      class="o-metadata__file-description"&gt;HLA-B and -C complexes
      were isolated using affinity chromatography using B1.23.2 from IFN-γ
      treated WT, TAPBPR stably depleted (shTAPBPR) and tapasin deficient (TPN-)
      KBM-7 cells. Eluted peptide were analysed using
      LC-MS/MS.&lt;/div&gt;&lt;div
      class="o-metadata__file-name"&gt;KBM7 elution from
      B1232.xlsx&lt;/br&gt;&lt;/div&gt;&lt;/div&gt;
    </description>
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