{"data":{"id":"10.5061/dryad.44j0zpcn7","type":"dois","attributes":{"doi":"10.5061/dryad.44j0zpcn7","prefix":"10.5061","suffix":"dryad.44j0zpcn7","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Qin, Sen","nameType":"Personal","givenName":"Sen","familyName":"Qin","affiliation":["Peking University"],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0000-0002-1356-453X","nameIdentifierScheme":"ORCID"}]}],"titles":[{"title":"Reconstitution of the multiple myeloma microenvironment following lymphodepletion with BCMA CAR-T therapy"}],"publisher":"Dryad","container":{},"publicationYear":2024,"subjects":[{"subject":"FOS: Basic medicine","schemeUri":"https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf","subjectScheme":"fos"},{"subject":"FOS: Basic medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Multiple myeloma","schemeUri":"https://github.com/PLOS/plos-thesaurus","subjectScheme":"PLOS Subject Area Thesaurus"},{"subject":"BCMA CAR-T Therapy"},{"subject":"Myeloma Microenvironment"}],"contributors":[],"dates":[{"date":"2024-03-16T12:19:01Z","dateType":"Created"},{"date":"2024-05-15T01:57:09Z","dateType":"Submitted"},{"date":"2024-05-21T00:00:00Z","dateType":"Issued"},{"date":"2024-05-21T00:00:00Z","dateType":"Available"}],"language":"en","types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementedBy","relatedIdentifier":"10.1158/1078-0432.c.7447774","relatedIdentifierType":"DOI"},{"relationType":"IsSourceOf","relatedIdentifier":"10.1158/1078-0432.27013651.v1","relatedIdentifierType":"DOI"},{"relationType":"IsCitedBy","relatedIdentifier":"10.1158/1078-0432.ccr-24-0352","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["3683200699 bytes"],"formats":[],"version":"4","rightsList":[{"rights":"Creative Commons Zero v1.0 Universal","rightsUri":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc0-1.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"BCMA-targeted CAR-T therapy has shown potent treatment outcomes in\n treating multiple myeloma (MM), a disease characterized by malignant bone\n marrow (BM) plasma cells. However, the remodeling of MM microenvironment\n after CAR-T therapy remains poorly understood. Here, we report the\n reconstitution of MM microenvironment by obtaining single-cell\n transcriptomes for paired BM specimens (n = 14) from 7 MM patients before\n (i.e., baseline, ''day −4'') and after (i.e.,\n ''day 28'') post-lymphodepleted BCMA CAR-T therapy.\n Our analysis revealed heterogeneity in driver gene expression among MM\n cells, even those harboring the same cytogenetic abnormalities. The best\n overall responses of patients over the 15-month follow-up are positively\n correlated with the abundance and targeted cytotoxic activity of CD8+\n effector CAR-T cells on day 28 after CAR-T cell infusion. Additionally,\n favorable responses are associated with attenuated immunosuppression\n mediated by regulatory T cells (Tregs), enhanced CD8+ effector T cell\n cytotoxic activity, and elevated type 1 conventional dendritic cell (cDC1)\n antigen presentation ability. DC re-clustering inferred\n intramedullary-originated cDC3s with extramedullary migration. Cell-cell\n communication network analysis indicated BCMA CAR-T therapy mitigates\n BAFF/GALECTIN/MK pathway-mediated immunosuppression and activates MIF\n pathway-mediated anti-MM immunity. Our study sheds light on MM\n microenvironment dynamics after BCMA CAR-T therapy, offering clues for\n predicting treatment responsivity.","descriptionType":"Abstract"},{"description":"The collected paired BM specimens from MM patients before and\n after BCMA CAR-T therapy were isolated into single cell suspensions, and\n 3'-scRNA-seq (Chromium Single Cell 3′ v3 Libraries) analysis was\n performed on each sample. The analysis included 7 MM\n patients (P1-P7). We collected baseline BM aspirate specimens (P1_B, P2_B,\n P3_B, P4_B, P5_B, P6_B, and P7_B) from each patient before\n (\u003cem\u003ei.e.\u003c/em\u003e, baseline, ''day −4'') BCMA\n CAR-T cell infusion (\u003cem\u003ei.e.\u003c/em\u003e, ''day\n 0''), and the BM aspirate specimens (P1_R, P2_R, P3_R, P4_R,\n P5_R, P6_R, and P7_R) after BCMA CAR-T therapy were collected on day 28.\n These patients had received 2 or 3 previous lines of therapies, including\n two patients (P2 and P6) with extramedullary disease; the other patients\n did not experience extramedullary progression. All patients received\n cyclophosphamide-mediated lymphodepletion on day −3 with the aim of\n potentiating the expansion of CAR-T cells. Efficacy assessments based on\n the International Myeloma Working Group (IMWG) criteria displayed that the\n patients had achieved varying degrees of remission by day 28: P3 achieved\n stringent complete remission (sCR); P1 and P4 achieved very good partial\n remission (VGPR); the other patients achieved partial remission\n (PR). The BM aspirate samples were collected during the\n diagnostic procedure. Bone marrow mononuclear cells (BMMCs) were isolated\n using Ficoll Paque Plus (Cytiva) and washed three times with Hanks\n Balanced Salt Solution (HBSS, Solarbio). Following centrifugation at 1,000\n rpm for 5 min, the supernatant was discarded, and the sediment was\n resuspended in 1 ml phosphate-buffered saline (PBS, Solarbio). To\n eliminate red blood cells, 1-2 ml red blood cell lysis buffer (BD) was\n added at 4°C for 10 min. After centrifugation at 500 g for 5 min, the\n cells were suspended in PBS. The cell viability was assessed using trypan\n blue (Sigma) and examined under a microscope. Utilizing\n the 10x Genomics Chromium Single Cell 3′ v3 Library Kit and Chromium\n instrument, approximately 17,500 cells were partitioned into nanoliter\n droplets to achieve single-cell resolution for a maximum of 10,000\n individual cells per sample. The resulting cDNA was tagged with a common\n 16 nt cell barcode and 10 nt Unique Molecular Identifier during the RT\n reaction. Full-length cDNA from poly-A mRNA transcripts was enzymatically\n fragmented and size selected to optimize the cDNA amplicon size\n (approximately 400 bp) for library construction (10x Genomics). The\n concentration of the 10x single-cell library was accurately determined\n through qPCR (Kapa Biosystems) to produce cluster counts appropriate for\n the HiSeq4000 or NovaSeq6000 platform (Illumina). In all, 26 × 98 bp (3′\n v3 libraries) sequence data were generated targeting between 25 and 50K\n read pairs/cell, which provided digital gene expression profiles for each\n individual cell. For single-cell RNA-seq analysis, we\n used CellRanger (10x Genomics, v.6.1.2) to pre-process the single-cell\n RNA-seq data after obtaining the paired-end raw reads. Cell barcodes and\n unique molecular identifiers (UMIs) of the library were extracted from\n read 1. Then, the reads were split according to their cell (barcode) IDs,\n and the UMI sequences from read 2 were simultaneously recorded for each\n cell. Quality control on these raw readings was subsequently performed to\n eliminate adapter contamination, duplicates, and low-quality bases. After\n filtering barcodes and low-quality readings that were not related to\n cells, we mapped the cleaned readings to the human genome (GRCh38) and\n retained the uniquely mapped readings for UMIs counts. Next, we estimated\n the accurate molecular counts and generated a UMI count matrix for each\n cell by counting UMIs for each sample. Finally, we generated a\n gene-barcode matrix that showed the barcoded cells and gene expression\n counts.","descriptionType":"Methods"},{"description":"# Reconstitution of the Multiple Myeloma Microenvironment Following\n Lymphodepletion with BCMA CAR-T Therapy\n [https://doi.org/10.5061/dryad.44j0zpcn7](https://doi.org/10.5061/dryad.44j0zpcn7) The collected paired BM specimens from MM patients before and after BCMA CAR-T therapy were isolated into single cell suspensions, and 3'-scRNA-seq (Chromium Single Cell 3' v3 Libraries) analysis was performed on each sample. ## Description of the data and file structure The dataset includes '.mtx' files, each of which is for creating a Seurat object in R. The naming convention of the '.mtx' files was following the sampling time points of the MM patients receiving BCMA CAR-T therapy. Specifically, the 14 BM specimens that performed scRNA-seq were collected from 7 relapsed or refractory MM patients (i.e. 'P1, P2, P3, P4, P5, P6, and P7') before and after BCMA CAR-T therapy. For these specimens, the baseline (i.e. 'B') specimens (P1_B, P2_B, P3_B, P4_B, P5_B, P6_B, and P7_B) were collected from P1-P7 on day -4 before BCMA CAR-T therapy, and the remission stage (i.e. 'R') specimens (P1_R, P2_R, P3_R, P4_R, P5_R, P6_R, and P7_R) were collected from P1-P7 on day 28 after BCMA CAR-T cell infusion. The '.mtx' files contained gene-barcode matrix that showed the barcoded cells and gene expression counts. In these matrices, each row represents a barcoded cell name, and each column represents a gene name. Thus, the analysis of the expression level of each gene in each cell was available. ## Sharing/Access information Human transcriptome reference used for our analysis is available at 10x Genomics website ([https://cf.10xgenomics.com/supp/cell-exp/refdata-cellranger-GRCh38-3.0.0.tar.gz](https://cf.10xgenomics.com/supp/cell-exp/refdata-cellranger-GRCh38-3.0.0.tar.gz)); the code used for scRNA-seq analysis is available at 10x Genomics website ([https://www.10xgenomics.com/support/software/cell-ranger/latest/analysis/running-pipelines/cr-gex-count](https://www.10xgenomics.com/support/software/cell-ranger/latest/analysis/running-pipelines/cr-gex-count) and at GitHub website ([https://github.com/satijalab/seurat/releases/tag/v4.0.2).](https://github.com/satijalab/seurat/releases/tag/v4.0.2\\).) ## Code/Software The single-cell RNA sequencing data was processed using cellranger (v.6.1.2) and analyzed with the R package Seurat (v.4.0.2). To analyze the data, firslty running the packages that provided in the code under the R Studio operating environment. Then, inputting all of the '.mtx' files for creating the Seurat object, for which the custom storage path of the downloaded data needing to be changed in the provided script to input the data. The downstream analyses can fully followed the provided code.","descriptionType":"TechnicalInfo"}],"geoLocations":[],"fundingReferences":[],"xml":"<?xml version="1.0" encoding="UTF-8"?>
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      <creatorName nameType="Personal">Qin, Sen</creatorName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0002-1356-453X</nameIdentifier>
      <affiliation affiliationIdentifier="https://ror.org/02v51f717" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Peking University</affiliation>
    </creator>
  </creators>
  <titles>
    <title>
      Reconstitution of the multiple myeloma microenvironment following
      lymphodepletion with BCMA CAR-T therapy
    </title>
  </titles>
  <publisher publisherIdentifier="https://ror.org/00x6h5n95" publisherIdentifierScheme="ROR" schemeURI="https://ror.org/">Dryad</publisher>
  <resourceType resourceTypeGeneral="Dataset">dataset</resourceType>
  <publicationYear>2024</publicationYear>
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    <subject subjectScheme="fos" schemeURI="https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf">FOS: Basic medicine</subject>
    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Multiple myeloma</subject>
    <subject>BCMA CAR-T Therapy</subject>
    <subject>Myeloma Microenvironment</subject>
  </subjects>
  <dates>
    <date dateType="Created">2024-03-16T12:19:01Z</date>
    <date dateType="Submitted">2024-05-15T01:57:09Z</date>
    <date dateType="Issued">2024-05-21T00:00:00Z</date>
    <date dateType="Available">2024-05-21T00:00:00Z</date>
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  <language>en</language>
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    <description descriptionType="Abstract">
      BCMA-targeted CAR-T therapy has shown potent treatment outcomes in
      treating multiple myeloma (MM), a disease characterized by malignant bone
      marrow (BM) plasma cells. However, the remodeling of MM microenvironment
      after CAR-T therapy remains poorly understood. Here, we report the
      reconstitution of MM microenvironment by obtaining single-cell
      transcriptomes for paired BM specimens (n = 14) from 7 MM patients before
      (i.e., baseline, ''day −4'') and after (i.e.,
      ''day 28'') post-lymphodepleted BCMA CAR-T therapy.
      Our analysis revealed heterogeneity in driver gene expression among MM
      cells, even those harboring the same cytogenetic abnormalities. The best
      overall responses of patients over the 15-month follow-up are positively
      correlated with the abundance and targeted cytotoxic activity of CD8+
      effector CAR-T cells on day 28 after CAR-T cell infusion. Additionally,
      favorable responses are associated with attenuated immunosuppression
      mediated by regulatory T cells (Tregs), enhanced CD8+ effector T cell
      cytotoxic activity, and elevated type 1 conventional dendritic cell (cDC1)
      antigen presentation ability. DC re-clustering inferred
      intramedullary-originated cDC3s with extramedullary migration. Cell-cell
      communication network analysis indicated BCMA CAR-T therapy mitigates
      BAFF/GALECTIN/MK pathway-mediated immunosuppression and activates MIF
      pathway-mediated anti-MM immunity. Our study sheds light on MM
      microenvironment dynamics after BCMA CAR-T therapy, offering clues for
      predicting treatment responsivity.
    </description>
    <description descriptionType="Methods">
      &lt;p&gt;The collected paired BM specimens from MM patients before and
      after BCMA CAR-T therapy were isolated into single cell suspensions, and
      3'-scRNA-seq (Chromium Single Cell 3′ v3 Libraries) analysis was
      performed on each sample.&lt;/p&gt; &lt;p&gt;The analysis included 7 MM
      patients (P1-P7). We collected baseline BM aspirate specimens (P1_B, P2_B,
      P3_B, P4_B, P5_B, P6_B, and P7_B) from each patient before
      (&lt;em&gt;i.e.&lt;/em&gt;, baseline, ''day −4'') BCMA
      CAR-T cell infusion (&lt;em&gt;i.e.&lt;/em&gt;, ''day
      0''), and the BM aspirate specimens (P1_R, P2_R, P3_R, P4_R,
      P5_R, P6_R, and P7_R) after BCMA CAR-T therapy were collected on day 28.
      These patients had received 2 or 3 previous lines of therapies, including
      two patients (P2 and P6) with extramedullary disease; the other patients
      did not experience extramedullary progression. All patients received
      cyclophosphamide-mediated lymphodepletion on day −3 with the aim of
      potentiating the expansion of CAR-T cells. Efficacy assessments based on
      the International Myeloma Working Group (IMWG) criteria displayed that the
      patients had achieved varying degrees of remission by day 28: P3 achieved
      stringent complete remission (sCR); P1 and P4 achieved very good partial
      remission (VGPR); the other patients achieved partial remission
      (PR).&lt;/p&gt; &lt;p&gt;The BM aspirate samples were collected during the
      diagnostic procedure. Bone marrow mononuclear cells (BMMCs) were isolated
      using Ficoll Paque Plus (Cytiva) and washed three times with Hanks
      Balanced Salt Solution (HBSS, Solarbio). Following centrifugation at 1,000
      rpm for 5 min, the supernatant was discarded, and the sediment was
      resuspended in 1 ml phosphate-buffered saline (PBS, Solarbio). To
      eliminate red blood cells, 1-2 ml red blood cell lysis buffer (BD) was
      added at 4°C for 10 min. After centrifugation at 500 g for 5 min, the
      cells were suspended in PBS. The cell viability was assessed using trypan
      blue (Sigma) and examined under a microscope.&lt;/p&gt; &lt;p&gt;Utilizing
      the 10x Genomics Chromium Single Cell 3′ v3 Library Kit and Chromium
      instrument, approximately 17,500 cells were partitioned into nanoliter
      droplets to achieve single-cell resolution for a maximum of 10,000
      individual cells per sample. The resulting cDNA was tagged with a common
      16 nt cell barcode and 10 nt Unique Molecular Identifier during the RT
      reaction. Full-length cDNA from poly-A mRNA transcripts was enzymatically
      fragmented and size selected to optimize the cDNA amplicon size
      (approximately 400 bp) for library construction (10x Genomics). The
      concentration of the 10x single-cell library was accurately determined
      through qPCR (Kapa Biosystems) to produce cluster counts appropriate for
      the HiSeq4000 or NovaSeq6000 platform (Illumina). In all, 26 × 98 bp (3′
      v3 libraries) sequence data were generated targeting between 25 and 50K
      read pairs/cell, which provided digital gene expression profiles for each
      individual cell.&lt;/p&gt; &lt;p&gt;For single-cell RNA-seq analysis, we
      used CellRanger (10x Genomics, v.6.1.2) to pre-process the single-cell
      RNA-seq data after obtaining the paired-end raw reads. Cell barcodes and
      unique molecular identifiers (UMIs) of the library were extracted from
      read 1. Then, the reads were split according to their cell (barcode) IDs,
      and the UMI sequences from read 2 were simultaneously recorded for each
      cell. Quality control on these raw readings was subsequently performed to
      eliminate adapter contamination, duplicates, and low-quality bases. After
      filtering barcodes and low-quality readings that were not related to
      cells, we mapped the cleaned readings to the human genome (GRCh38) and
      retained the uniquely mapped readings for UMIs counts. Next, we estimated
      the accurate molecular counts and generated a UMI count matrix for each
      cell by counting UMIs for each sample. Finally, we generated a
      gene-barcode matrix that showed the barcoded cells and gene expression
      counts.&lt;/p&gt;
    </description>
    <description descriptionType="TechnicalInfo">
      # Reconstitution of the Multiple Myeloma Microenvironment Following
      Lymphodepletion with BCMA CAR-T Therapy
      [https://doi.org/10.5061/dryad.44j0zpcn7](https://doi.org/10.5061/dryad.44j0zpcn7) The collected paired BM specimens from MM patients before and after BCMA CAR-T therapy were isolated into single cell suspensions, and 3'-scRNA-seq (Chromium Single Cell 3' v3 Libraries) analysis was performed on each sample. ## Description of the data and file structure The dataset includes '.mtx' files, each of which is for creating a Seurat object in R. The naming convention of the '.mtx' files was following the sampling time points of the MM patients receiving BCMA CAR-T therapy. Specifically, the 14 BM specimens that performed scRNA-seq were collected from 7 relapsed or refractory MM patients (i.e. 'P1, P2, P3, P4, P5, P6, and P7') before and after BCMA CAR-T therapy. For these specimens, the baseline (i.e. 'B') specimens (P1_B, P2_B, P3_B, P4_B, P5_B, P6_B, and P7_B) were collected from P1-P7 on day -4 before BCMA CAR-T therapy, and the remission stage (i.e. 'R') specimens (P1_R, P2_R, P3_R, P4_R, P5_R, P6_R, and P7_R) were collected from P1-P7 on day 28 after BCMA CAR-T cell infusion. The '.mtx' files contained gene-barcode matrix that showed the barcoded cells and gene expression counts. In these matrices, each row represents a barcoded cell name, and each column represents a gene name. Thus, the analysis of the expression level of each gene in each cell was available. ## Sharing/Access information Human transcriptome reference used for our analysis is available at 10x Genomics website ([https://cf.10xgenomics.com/supp/cell-exp/refdata-cellranger-GRCh38-3.0.0.tar.gz](https://cf.10xgenomics.com/supp/cell-exp/refdata-cellranger-GRCh38-3.0.0.tar.gz)); the code used for scRNA-seq analysis is available at 10x Genomics website ([https://www.10xgenomics.com/support/software/cell-ranger/latest/analysis/running-pipelines/cr-gex-count](https://www.10xgenomics.com/support/software/cell-ranger/latest/analysis/running-pipelines/cr-gex-count) and at GitHub website ([https://github.com/satijalab/seurat/releases/tag/v4.0.2).](https://github.com/satijalab/seurat/releases/tag/v4.0.2\).) ## Code/Software The single-cell RNA sequencing data was processed using cellranger (v.6.1.2) and analyzed with the R package Seurat (v.4.0.2). To analyze the data, firslty running the packages that provided in the code under the R Studio operating environment. Then, inputting all of the '.mtx' files for creating the Seurat object, for which the custom storage path of the downloaded data needing to be changed in the provided script to input the data. The downstream analyses can fully followed the provided code.
    </description>
  </descriptions>
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