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At subcytotoxic concentrations, PFOA impacted expression of\n hundreds of genes, including several molecules (CRH, IFIT1, and TNFSF10)\n linked with lipid metabolism and innate immune response pathways.\n Furthermore, in silico analyses suggested that regulatory factors such as\n peroxisome proliferator-activated receptor (PPAR)-mediated pathways may be\n especially important in response to PFOA. In summary, this study provides\n evidence that PFOA alters primary human CTB viability and gene pathways\n that could contribute to placental dysfunction and disease.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[{"schemeUri":"https://ror.org","funderName":"National Institute of Environmental Health Sciences","funderIdentifier":"https://ror.org/00j4k1h63","funderIdentifierType":"ROR"}],"xml":"<?xml version="1.0" encoding="UTF-8"?>
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    <title>
      Perfluorooctanoic acid induces transcriptomic alterations in 2nd trimester
      human cytotrophoblasts
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    <date dateType="Issued">2023-04-20T00:00:00Z</date>
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    <description descriptionType="Abstract">
      Poly- and perfluroroalkylated substances (PFAS) are a major class of
      surfactants used in industry applications and consumer products. Despite
      efforts to reduce the usage of PFAS due to their environmental
      persistence, compounds such as perfluorooctanoic acid (PFOA) are widely
      detected in human blood and tissue. While growing evidence supports that
      prenatal exposures to PFOA and other PFAS are linked to adverse pregnancy
      outcomes, the target organs and pathways remain unclear. Recent
      investigations in mouse and human cell lines suggest that PFAS may impact
      the placenta and impair trophoblast function. In this study, we
      investigated the effects of PFOA on cytotoxicity and the transcriptome in
      cultured 2nd-trimester human CTBs. We show that PFOA significantly reduces
      viability and induces cell death at 24h, in a concentration-dependent
      manner. At subcytotoxic concentrations, PFOA impacted expression of
      hundreds of genes, including several molecules (CRH, IFIT1, and TNFSF10)
      linked with lipid metabolism and innate immune response pathways.
      Furthermore, in silico analyses suggested that regulatory factors such as
      peroxisome proliferator-activated receptor (PPAR)-mediated pathways may be
      especially important in response to PFOA. In summary, this study provides
      evidence that PFOA alters primary human CTB viability and gene pathways
      that could contribute to placental dysfunction and disease.
    </description>
  </descriptions>
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