{"data":{"id":"10.5061/dryad.280gb5mpw","type":"dois","attributes":{"doi":"10.5061/dryad.280gb5mpw","prefix":"10.5061","suffix":"dryad.280gb5mpw","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Pace, Nikolai","nameType":"Personal","givenName":"Nikolai","familyName":"Pace","affiliation":["University of Malta"],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0000-0002-7332-874X","nameIdentifierScheme":"ORCID"}]}],"titles":[{"title":"Acute effects on the human peripheral blood transcriptome of decompression sickness secondary to scuba diving: Supplementary datasets"}],"publisher":"Dryad","container":{},"publicationYear":2021,"subjects":[{"subject":"FOS: Basic medicine","schemeUri":"https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf","subjectScheme":"fos"},{"subject":"FOS: Basic medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Hyperbaric physiology"},{"subject":"decompression sickness"},{"subject":"RNA sequencing","schemeUri":"https://github.com/PLOS/plos-thesaurus","subjectScheme":"PLOS Subject Area Thesaurus"}],"contributors":[],"dates":[{"date":"2021-04-13T12:20:03Z","dateType":"Submitted"},{"date":"2021-06-02T00:00:00Z","dateType":"Issued"},{"date":"2021-06-02T00:00:00Z","dateType":"Available"}],"language":"en","types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsCitedBy","relatedIdentifier":"10.3389/fphys.2021.660402","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["76229173 bytes"],"formats":[],"version":"5","rightsList":[{"rights":"Creative Commons Zero v1.0 Universal","rightsUri":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc0-1.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Decompression sickness (DCS) develops due to inert gas bubble formation in\n the circulation, leading to a wide range of potentially serious clinical\n manifestations. Its pathophysiology remains incompletely understood. In\n this study, we aim to explore changes in the human leukocyte transcriptome\n in divers with cutaneous DCS compared to closely matched unaffected\n controls after uneventful diving. Cases (n = 7) were divers developing the\n typical cutis marmorata rash after diving with a confirmed clinical\n diagnosis of DCS. Controls (n = 6) were healthy divers who surfaced from a\n ≥25 msw dive without decompression violation or evidence of DCS. Blood was\n sampled at two separate time points – within 8 hours of dive completion\n and 40-44 hours later. Transcriptome analysis by RNA-Sequencing followed\n by bioinformatic analysis was carried out to identify differentially\n expressed genes and relate their function to biological pathways. In DCS\n cases, we identified enrichment of transcripts involved in acute\n inflammation, activation of innate immunity and free radical scavenging\n pathways, with specific upregulation of transcripts related to neutrophil\n function and degranulation. DCS-induced transcriptomic events were\n reversed at the second time point following exposure to hyperbaric oxygen.\n The observed changes are consistent with findings from animal models of\n DCS and highlight a continuum between the responses elicited by uneventful\n diving and diving complicated by DCS. This study sheds light on the\n inflammatory pathophysiology of DCS and the associated immune response.\n Such data may potentially be valuable in the search for novel treatments\n targeting this disease.  ","descriptionType":"Abstract"},{"description":"In this study, we aim to explore the evolution of leukocyte gene\n expression in human subjects with DCS compared to closely matched divers\n after uneventful diving using a hypothesis-free RNA sequencing approach\n .Recruitment of DCS cases (n = 7) and controls (n = 6) was carried out\n using specific criteria. For both DCS cases and controls, whole blood was\n sampled at two time points, T1 - within 8 hours of surfacing from diving\n and T2 - at 40-44 hours after surfacing. Prior to sampling at T2, subjects\n were fasted for 10 hours. Specific exclusion criteria included a) age\n \u0026lt; 18 years b) self-reported consumption of alcohol and/or strenuous\n physical activity before T2 c) symptoms suggestive of delayed DCS\n presentation in controls at T2 d) acute life-threatening clinical\n complications or death within 72 hours of surfacing. All cases received\n emergency HBO as per United States Navy Treatment Table Six between T1 and\n T2.For RNA isolation, 2.5mL of whole blood was collected in a PAXgene®\n Blood RNA Tube (PreAnalytiX, Qiagen/BD) from DCS cases and controls at\n both T1 and T2. The quality of RNA was evaluated by RNA Integrity Number\n (RIN) determination using the RNA6000 Nano protocol on an Agilent 2100\n Bioanalyzer system (Agilent, USA). The RIN values for samples undergoing\n transcriptome analysis ranged from 7.8 to 9.3. Depletion of alpha and beta\n globin mRNA was carried out using the GLOBINclear™ kit (ThermoFisher\n Scientific). To minimize batch effects, all samples were processed\n simultaneously by the same investigator. RNA samples were submitted for\n library generation and sequencing by the Beijing Genomics Institute\n (BGI-Shenzhen). Briefly, poly(A) mRNA was enriched using poly(T)\n oligo-attached magnetic beads, followed by fragmentation. First strand\n cDNA synthesis was carried out using random hexamer N6 primers and reverse\n transcriptase. Following adaptor ligation to cDNA fragments, PCR\n amplification and purification, single stranded DNA circles were generated\n in a final library. DNA nanoballs (DNBs) were subsequently generated by\n rolling circle replication, which underwent paired end sequencing (100bp)\n on the BGI DNBseq platform","descriptionType":"Methods"},{"description":"The readme file contains an explanation of each of the files in\n the dataset.","descriptionType":"Other"}],"geoLocations":[],"fundingReferences":[{"schemeUri":"https://ror.org","funderName":"University of Malta","funderIdentifier":"https://ror.org/03a62bv60","funderIdentifierType":"ROR"}],"xml":"<?xml version="1.0" encoding="UTF-8"?>
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  <identifier identifierType="DOI">10.5061/DRYAD.280GB5MPW</identifier>
  <creators>
    <creator>
      <creatorName nameType="Personal">Pace, Nikolai</creatorName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0002-7332-874X</nameIdentifier>
      <affiliation affiliationIdentifier="https://ror.org/03a62bv60" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of Malta</affiliation>
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  <titles>
    <title>
      Acute effects on the human peripheral blood transcriptome of decompression
      sickness secondary to scuba diving: Supplementary datasets
    </title>
  </titles>
  <publisher publisherIdentifier="https://ror.org/00x6h5n95" publisherIdentifierScheme="ROR" schemeURI="https://ror.org/">Dryad</publisher>
  <resourceType resourceTypeGeneral="Dataset">dataset</resourceType>
  <publicationYear>2021</publicationYear>
  <subjects>
    <subject subjectScheme="fos" schemeURI="https://web-archive.oecd.org/2012-06-15/138575-38235147.pdf">FOS: Basic medicine</subject>
    <subject>Hyperbaric physiology</subject>
    <subject>decompression sickness</subject>
    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">RNA sequencing</subject>
  </subjects>
  <fundingReferences>
    <fundingReference>
      <funderName>University of Malta</funderName>
      <funderIdentifier funderIdentifierType="ROR">https://ror.org/03a62bv60</funderIdentifier>
    </fundingReference>
  </fundingReferences>
  <dates>
    <date dateType="Submitted">2021-04-13T12:20:03Z</date>
    <date dateType="Issued">2021-06-02T00:00:00Z</date>
    <date dateType="Available">2021-06-02T00:00:00Z</date>
  </dates>
  <language>en</language>
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  <descriptions>
    <description descriptionType="Abstract">
      Decompression sickness (DCS) develops due to inert gas bubble formation in
      the circulation, leading to a wide range of potentially serious clinical
      manifestations. Its pathophysiology remains incompletely understood. In
      this study, we aim to explore changes in the human leukocyte transcriptome
      in divers with cutaneous DCS compared to closely matched unaffected
      controls after uneventful diving. Cases (n = 7) were divers developing the
      typical cutis marmorata rash after diving with a confirmed clinical
      diagnosis of DCS. Controls (n = 6) were healthy divers who surfaced from a
      ≥25 msw dive without decompression violation or evidence of DCS. Blood was
      sampled at two separate time points – within 8 hours of dive completion
      and 40-44 hours later. Transcriptome analysis by RNA-Sequencing followed
      by bioinformatic analysis was carried out to identify differentially
      expressed genes and relate their function to biological pathways. In DCS
      cases, we identified enrichment of transcripts involved in acute
      inflammation, activation of innate immunity and free radical scavenging
      pathways, with specific upregulation of transcripts related to neutrophil
      function and degranulation. DCS-induced transcriptomic events were
      reversed at the second time point following exposure to hyperbaric oxygen.
      The observed changes are consistent with findings from animal models of
      DCS and highlight a continuum between the responses elicited by uneventful
      diving and diving complicated by DCS. This study sheds light on the
      inflammatory pathophysiology of DCS and the associated immune response.
      Such data may potentially be valuable in the search for novel treatments
      targeting this disease. &amp;nbsp;
    </description>
    <description descriptionType="Methods">
      &lt;p&gt;In this study, we aim to explore the evolution of leukocyte gene
      expression in human subjects with DCS compared to closely matched divers
      after uneventful diving using a hypothesis-free RNA sequencing approach
      .Recruitment of DCS cases (n = 7) and controls (n = 6) was carried out
      using specific criteria. For both DCS cases and controls, whole blood was
      sampled at two time points, T1 - within 8 hours of surfacing from diving
      and T2 - at 40-44 hours after surfacing. Prior to sampling at T2, subjects
      were fasted for 10 hours. Specific exclusion criteria included a) age
      &amp;lt; 18 years b) self-reported consumption of alcohol and/or strenuous
      physical activity before T2 c) symptoms suggestive of delayed DCS
      presentation in controls at T2 d) acute life-threatening clinical
      complications or death within 72 hours of surfacing. All cases received
      emergency HBO as per United States Navy Treatment Table Six between T1 and
      T2.For RNA isolation, 2.5mL of whole blood was collected in a PAXgene®
      Blood RNA Tube (PreAnalytiX, Qiagen/BD) from DCS cases and controls at
      both T1 and T2. The quality of RNA was evaluated by RNA Integrity Number
      (RIN) determination using the RNA6000 Nano protocol on an Agilent 2100
      Bioanalyzer system (Agilent, USA). The RIN values for samples undergoing
      transcriptome analysis ranged from 7.8 to 9.3. Depletion of alpha and beta
      globin mRNA was carried out using the GLOBINclear™ kit (ThermoFisher
      Scientific). To minimize batch effects, all samples were processed
      simultaneously by the same investigator. RNA samples were submitted for
      library generation and sequencing by the Beijing Genomics Institute
      (BGI-Shenzhen). Briefly, poly(A) mRNA was enriched using poly(T)
      oligo-attached magnetic beads, followed by fragmentation. First strand
      cDNA synthesis was carried out using random hexamer N6 primers and reverse
      transcriptase. Following adaptor ligation to cDNA fragments, PCR
      amplification and purification, single stranded DNA circles were generated
      in a final library. DNA nanoballs (DNBs) were subsequently generated by
      rolling circle replication, which underwent paired end sequencing (100bp)
      on the BGI DNBseq platform&lt;/p&gt;
    </description>
    <description descriptionType="Other">
      &lt;p&gt;The readme file contains an explanation of each of the files in
      the dataset.&lt;/p&gt;
    </description>
  </descriptions>
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