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    <title>
      Data from: Gene transcription in sea otters (Enhydra lutris): development
      of a diagnostic tool for sea otter and ecosystem health
    </title>
  </titles>
  <publisher publisherIdentifier="https://ror.org/00x6h5n95" publisherIdentifierScheme="ROR" schemeURI="https://ror.org/">Dryad</publisher>
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    <subject>Disease Biology</subject>
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    <date dateType="Submitted">2011-07-18T22:38:42Z</date>
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    <description descriptionType="Abstract">
      Gene transcription analysis for diagnosing or monitoring wildlife health
      requires the ability to distinguish pathophysiological change from natural
      variation. Herein we describe methodology for the development of
      quantitative real time polymerase chain reaction (qPCR) assays to measure
      differential transcript levels of multiple immune-function genes in the
      sea otter (Enhydra lutris); sea otter specific, qPCR primer sequences for
      the genes of interest are defined. We establish a “reference” range of
      transcripts for each gene in a group of clinically healthy captive and
      free-ranging sea otters. The 10 genes of interest represent multiple
      physiological systems that play a role in immuno-modulation, inflammation,
      cell protection, tumor suppression, cellular stress-response, xenobiotic
      metabolizing enzymes, antioxidant enzymes, and cell-cell adhesion. The
      cycle threshold (CT) measures for most genes were normally distributed;
      the complement cytolysis inhibitor was the exception. The relative
      enumeration of multiple gene transcripts in simple peripheral blood
      samples expands the diagnostic capability currently available to assess
      the health of sea otters in situ and provides a better understanding of
      the state of their environment.
    </description>
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      &lt;div class="o-metadata__file-usage-entry"&gt;&lt;h4
      class="o-heading__level3-file-title"&gt;Figure 1 and Table 4
      71811&lt;/h4&gt;&lt;div
      class="o-metadata__file-name"&gt;&lt;/div&gt;&lt;/div&gt;
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