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  <identifier identifierType="DOI">10.5061/DRYAD.1HG75</identifier>
  <creators>
    <creator>
      <creatorName nameType="Personal">Brelsford, Alan</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/019whta54" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of Lausanne</affiliation>
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    <creator>
      <creatorName nameType="Personal">Collin, Hélène</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/019whta54" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of Lausanne</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Perrin, Nicolas</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/019whta54" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of Lausanne</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Fumagalli, Luca</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/019whta54" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of Lausanne</affiliation>
    </creator>
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  <titles>
    <title>
      Data from: Nonspecific PCR amplification by high-fidelity polymerases:
      implications for next-generation sequencing of AFLP markers.
    </title>
  </titles>
  <publisher publisherIdentifier="https://ror.org/00x6h5n95" publisherIdentifierScheme="ROR" schemeURI="https://ror.org/">Dryad</publisher>
  <resourceType resourceTypeGeneral="Dataset">dataset</resourceType>
  <publicationYear>2011</publicationYear>
  <subjects>
    <subject>Oryza sativa</subject>
    <subject>proofreading polymerase</subject>
    <subject>high-fidelity polymerase</subject>
    <subject>454</subject>
    <subject>AFLP</subject>
    <subject>Phoxinus phoxinus</subject>
  </subjects>
  <dates>
    <date dateType="Submitted">2011-08-03T13:55:55Z</date>
    <date dateType="Issued">2011-08-03T15:52:32Z</date>
    <date dateType="Available">2011-08-03T15:52:32Z</date>
  </dates>
  <language>en</language>
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    <description descriptionType="Abstract">
      High-fidelity “proofreading” polymerases are often used in library
      construction for next-generation sequencing projects, in an effort to
      minimize errors in the resulting sequence data. The increased template
      fidelity of these polymerases can come at the cost of reduced template
      specificity, and library preparation methods based on the AFLP technique
      may be particularly susceptible. Here, we compare AFLP profiles generated
      with standard Taq and two versions of a high-fidelity polymerase. We find
      that Taq produces fewer and brighter peaks than high-fidelity polymerase,
      suggesting that Taq performs better at selectively amplifying templates
      that exactly match the primer sequences. Because the higher accuracy of
      proofreading polymerases remains important for sequencing applications, we
      suggest that it may be more effective to use alternative library
      preparation methods.
    </description>
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      class="o-heading__level3-file-title"&gt;FishPeakArea&lt;/h4&gt;&lt;div class="o-metadata__file-description"&gt;Peak area calculated by Genemapper from ABI sequencer chromatograms for European minnow samples. Each column labelled "Peak X bp" is an AFLP locus with a fragment length of X base pairs. In the column "Polymerase", "PHU" refers to Phusion, "HOT" to Phusion Hot Start II, and "TAQ" to standard Taq polymerase.&lt;/div&gt;&lt;div class="o-metadata__file-name"&gt;&lt;/div&gt;&lt;/div&gt;&lt;div class="o-metadata__file-usage-entry"&gt;&lt;h4 class="o-heading__level3-file-title"&gt;FishPeakHeight&lt;/h4&gt;&lt;div class="o-metadata__file-description"&gt;Peak height in Relative Fluorescent Units (RFU) calculated by Genemapper from ABI sequencer chromatograms for European minnow samples. Each column labelled "Peak X bp" is an AFLP locus with a fragment length of X base pairs. In the column "Polymerase", "PHU" refers to Phusion, "HOT" to Phusion Hot Start II, and "TAQ" to standard Taq polymerase.&lt;/div&gt;&lt;div class="o-metadata__file-name"&gt;&lt;/div&gt;&lt;/div&gt;&lt;div class="o-metadata__file-usage-entry"&gt;&lt;h4 class="o-heading__level3-file-title"&gt;RicePeakArea&lt;/h4&gt;&lt;div class="o-metadata__file-description"&gt;Peak area calculated by Genemapper from ABI sequencer chromatograms for rice samples. Each column labelled "Peak X bp" is an AFLP locus with a fragment length of X base pairs. In the column "Polymerase", "PHU" refers to Phusion, "HOT" to Phusion Hot Start II, and "TAQ" to standard Taq polymerase.&lt;/div&gt;&lt;div class="o-metadata__file-name"&gt;&lt;/div&gt;&lt;/div&gt;&lt;div class="o-metadata__file-usage-entry"&gt;&lt;h4 class="o-heading__level3-file-title"&gt;RicePeakHeight&lt;/h4&gt;&lt;div class="o-metadata__file-description"&gt;Peak height in Relative Fluorescent Units (RFU) calculated by Genemapper from ABI sequencer chromatograms for rice samples. Each column labelled "Peak X bp" is an AFLP locus with a fragment length of X base pairs. In the column "Polymerase", "PHU" refers to Phusion, "HOT" to Phusion Hot Start II, and "TAQ" to standard Taq polymerase.&lt;/div&gt;&lt;div class="o-metadata__file-name"&gt;&lt;/div&gt;&lt;/div&gt;
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