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Here we show that NHA2\n localizes almost exclusively to distal convoluted tubules in the kidney.\n NHA2 knock-out mice displayed reduced blood pressure, normocalcemic\n hypocalciuria and an attenuated response to the thiazide diuretic\n hydrochlorothiazide. Phosphorylation of the thiazide-sensitive\n sodium/chloride cotransporter NCC and its upstream activating kinase\n Ste20/SPS1-related proline/alaninerich kinase (SPAK), as well as the\n abundance of with no lysine [K] kinase 4 (WNK4), were significantly\n reduced in kidneys of NHA2 knock-out mice. In vitro experiments\n recapitulated these findings and revealed increased WNK4 ubiquitylation\n and enhanced proteasomal WNK4 degradation upon loss of NHA2. The effect of\n NHA2 on WNK4 stability was Kelch-like 3 (KLHL3)-dependent. More\n specifically, loss of NHA2 selectively attenuated KLHL3 phosphorylation\n and the physiologically important PKAand PKC-mediated decrease of WNK4\n degradation was blunted upon loss of NHA2. Phenotype analysis of NHA2/NCC\n double knock-out mice supported the notion that NHA2 affects blood\n pressure homeostasis by an intrarenal and NCC-dependent mechanism. In\n summary, our data reveal NHA2 as a critical component of the WNK4-NCC\n pathway and hence as a novel regulator of blood pressure homeostasis in\n the kidney.","descriptionType":"Abstract"},{"description":"Detailed description of methods can be found in the original,\n published manuscript\n (https://pubmed.ncbi.nlm.nih.gov/32956652/). 1.\n Uncropped immunoblots of all immunoblots published in the manuscript\n (incl. Supplemental Information). For details of SDS-PAGE and\n immunoblotting see Materials and Methods section of the published\n manuscript. Developed X-ray films were scanned. Unedited scans were safed\n as TIF files and compiled in a Word Document. 2. Basic\n data of normalized expression (immunoblots or real-time PCR, for details\n of quantification see Materials and Methods section of the published\n manuscript) were imported in Graph Pad Prism (Version 9) for statistical\n analysis and visualization.","descriptionType":"Methods"},{"description":"A readme.txt file with detailed re-use instructions has been\n uploaded. 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      <creatorName nameType="Personal">Anderegg, Manuel</creatorName>
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      <creatorName nameType="Personal">Albano, Giuseppe</creatorName>
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      <creatorName nameType="Personal">Uehlinger, Dominik E.</creatorName>
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    <creator>
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    <creator>
      <creatorName nameType="Personal">Bhardwaj, Rajesh</creatorName>
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    <title>
      The sodium/proton exchanger NHA2 regulates blood pressure through a
      WNK4-NCC dependent pathway in the kidney
    </title>
  </titles>
  <publisher publisherIdentifier="https://ror.org/00x6h5n95" publisherIdentifierScheme="ROR" schemeURI="https://ror.org/">Dryad</publisher>
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  <dates>
    <date dateType="Submitted">2020-12-04T08:19:02Z</date>
    <date dateType="Issued">2020-12-10T00:00:00Z</date>
    <date dateType="Available">2020-12-10T00:00:00Z</date>
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  <descriptions>
    <description descriptionType="Abstract">
      NHA2 is sodium/hydrogen exchanger that was associated with arterial
      hypertension in humans, but the role of NHA2 in kidney function and blood
      pressure homeostasis is currently unknown. Here we show that NHA2
      localizes almost exclusively to distal convoluted tubules in the kidney.
      NHA2 knock-out mice displayed reduced blood pressure, normocalcemic
      hypocalciuria and an attenuated response to the thiazide diuretic
      hydrochlorothiazide. Phosphorylation of the thiazide-sensitive
      sodium/chloride cotransporter NCC and its upstream activating kinase
      Ste20/SPS1-related proline/alaninerich kinase (SPAK), as well as the
      abundance of with no lysine [K] kinase 4 (WNK4), were significantly
      reduced in kidneys of NHA2 knock-out mice. In vitro experiments
      recapitulated these findings and revealed increased WNK4 ubiquitylation
      and enhanced proteasomal WNK4 degradation upon loss of NHA2. The effect of
      NHA2 on WNK4 stability was Kelch-like 3 (KLHL3)-dependent. More
      specifically, loss of NHA2 selectively attenuated KLHL3 phosphorylation
      and the physiologically important PKAand PKC-mediated decrease of WNK4
      degradation was blunted upon loss of NHA2. Phenotype analysis of NHA2/NCC
      double knock-out mice supported the notion that NHA2 affects blood
      pressure homeostasis by an intrarenal and NCC-dependent mechanism. In
      summary, our data reveal NHA2 as a critical component of the WNK4-NCC
      pathway and hence as a novel regulator of blood pressure homeostasis in
      the kidney.
    </description>
    <description descriptionType="Methods">
      &lt;p&gt;Detailed description of methods can be found in the original,
      published manuscript
      (https://pubmed.ncbi.nlm.nih.gov/32956652/).&lt;/p&gt; &lt;p&gt;1.
      Uncropped immunoblots of all immunoblots published in the manuscript
      (incl. Supplemental Information). For details of SDS-PAGE and
      immunoblotting see Materials and Methods section of the published
      manuscript. Developed X-ray films were scanned. Unedited scans were safed
      as TIF files and compiled in a Word Document.&lt;/p&gt; &lt;p&gt;2. Basic
      data of normalized expression (immunoblots or real-time PCR, for details
      of quantification see Materials and Methods section of the published
      manuscript) were imported in Graph Pad Prism (Version 9) for statistical
      analysis and visualization.&lt;/p&gt;
    </description>
    <description descriptionType="Other">
      &lt;p&gt;A readme.txt file with detailed re-use instructions has been
      uploaded.&lt;/p&gt; &lt;p&gt;Uncropped blots can be visualized using Word
      (Version 2003 or higher), derived expression data can be visualized by
      Graph Pad Prism (Version 9 or higher).&lt;/p&gt;
    </description>
  </descriptions>
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