{"data":{"id":"10.4121/89ba9ee3-f072-4dda-97ba-683cce975a3b","type":"dois","attributes":{"doi":"10.4121/89ba9ee3-f072-4dda-97ba-683cce975a3b","prefix":"10.4121","suffix":"89ba9ee3-f072-4dda-97ba-683cce975a3b","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Pascale Daran-Lapujade","nameType":"Personal","nameIdentifiers":[{"nameIdentifier":"0000-0002-4097-7831","nameIdentifierScheme":"https://orcid.org/"}],"affiliation":[]},{"name":"Miriam Cammaert","givenName":"Miriam","familyName":"Cammaert","affiliation":[],"nameIdentifiers":[]},{"name":"Martin Pabst","nameType":"Personal","givenName":"Martin","familyName":"Pabst","nameIdentifiers":[{"nameIdentifier":"0000-0001-9897-0723","nameIdentifierScheme":"https://orcid.org/"}],"affiliation":[]},{"name":"Jitske van Ede","nameType":"Personal","givenName":"Jitske","familyName":"van Ede","nameIdentifiers":[{"nameIdentifier":"0009-0000-3503-8939","nameIdentifierScheme":"https://orcid.org/"}],"affiliation":[]},{"name":"Patricia van Dam","givenName":"Patricia","familyName":"van Dam","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Data supporting 'A protocol for lab-scale production of 13C yeast extract as internal standard for metabolomics and quantification of intracellular metabolites'"}],"publisher":"4TU.ResearchData","container":{},"publicationYear":2026,"subjects":[{"subject":"Biochemistry and Cell Biology","subjectScheme":"Australian and New Zealand Standard Research Classification (ANZSRC), 2008","classificationCode":"0601"},{"subject":"Biological Sciences","subjectScheme":"Australian and New Zealand Standard Research Classification (ANZSRC), 2008","classificationCode":"06"},{"subject":"Internal standards"},{"subject":"Fed-batch fermentation"},{"subject":"13C-yeast extract"},{"subject":"Metabolomics"},{"subject":"Mass spectrometry"}],"contributors":[{"name":"TU Delft, Faculty of Applied Sciences, Department of Biotechnology, Industrial Microbiology","nameType":"Organizational","contributorType":"Other","affiliation":[],"nameIdentifiers":[]}],"dates":[{"date":"2026-06-29","dateType":"Issued"}],"language":"en","types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsVersionOf","relatedIdentifier":"10.4121/89ba9ee3-f072-4dda-97ba-683cce975a3b.v1","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":[],"formats":["zipped shape files; spreadsheet/.xlsx; readme/.txt"],"version":"1","rightsList":[{"rights":"Creative Commons Attribution Non Commercial 4.0 International","rightsUri":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-nc-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Metabolomics enables the profiling of small-molecule metabolites and thereby captures the biochemical state of a living organism at a given moment and enables to monitor its cellular responses to stimuli. This technique has become a powerful tool in pharmaceutical research, the food industry, and microbial research. Metabolomics aims to obtain an unbiased metabolic profile; however, this is complicated by compound instability, complex and often extensive sample processing, and nonlinear responses in mass spectrometry. Therefore, correcting for metabolite loss and mass spectrometry-related artifacts is essential, typically achieved through relative quantification against an isotopically labelled internal standard for each metabolite of interest. This article describes how to produce \u003csup\u003e13\u003c/sup\u003eC-labelled yeast extract and its use as internal standard for metabolomics. More specifically, it provides step-by-step protocols for the fed-batch fermentation, quenching, metabolite extraction, and LC-MS and GC-MS characterization of the internal standard. It also includes a protocol explaining how to use the internal standard for the quantification of metabolites in yeast 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