{"data":{"id":"10.25402/fsoa.c.4443026","type":"dois","attributes":{"doi":"10.25402/fsoa.c.4443026","prefix":"10.25402","suffix":"fsoa.c.4443026","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Hardy, John G","nameType":"Personal","givenName":"John G","familyName":"Hardy","affiliation":[],"nameIdentifiers":[]},{"name":"Hetian Li","affiliation":[],"nameIdentifiers":[]},{"name":"Chow, Jacqueline K","nameType":"Personal","givenName":"Jacqueline K","familyName":"Chow","affiliation":[],"nameIdentifiers":[]},{"name":"Geissler, Sydney A","nameType":"Personal","givenName":"Sydney A","familyName":"Geissler","affiliation":[],"nameIdentifiers":[]},{"name":"McElroy, Austin B","nameType":"Personal","givenName":"Austin B","familyName":"McElroy","affiliation":[],"nameIdentifiers":[]},{"name":"Nguy, Lindsey","nameType":"Personal","givenName":"Lindsey","familyName":"Nguy","affiliation":[],"nameIdentifiers":[]},{"name":"Hernandez, Derek S","nameType":"Personal","givenName":"Derek S","familyName":"Hernandez","affiliation":[],"nameIdentifiers":[]},{"name":"Schmidt, Christine E","nameType":"Personal","givenName":"Christine E","familyName":"Schmidt","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Supplementary Information for: Conducting polymer-based multilayer films for instructive biomaterial coatings"}],"publisher":"Figshare","container":{},"publicationYear":2019,"subjects":[{"subject":"60106 Cellular Interactions (incl. Adhesion, Matrix, Cell Wall)","subjectScheme":"FOR"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"111601 Cell Physiology","subjectScheme":"FOR"},{"subject":"FOS: Basic medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Basic medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"60113 Synthetic Biology","subjectScheme":"FOR"}],"contributors":[],"dates":[{"date":"2019-03-21","dateType":"Created"},{"date":"2019-03-21","dateType":"Updated"},{"date":"2019","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.4155/fso.15.79","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"CC BY 4.0","rightsUri":"https://creativecommons.org/licenses/by/4.0"}],"descriptions":[{"description":"\u003cb\u003eAbstract\u003c/b\u003e\n  Cells inhabit environments\n  known as the extracellular matrix (ECM) which consists of a mixture of\n  different biomolecules, and the precise composition and topographical\n  properties are different in different tissues (e.g., bone, brain, muscle,\n  skin). Cells interact intimately with the ECM, not only constructing the\n  biomolecules, but assist its organization in 3D space, and its degradation\n  (which is important for tissue remodeling); reciprocally, cells respond to\n  the ECM (e.g., by modifying their size, shape, etc). Cellular alignment is\n  observed in organs and tissues such as bones, muscles and skin, and this\n  alignment is important for the healthy functioning of the organ/tissue. Here,\n  we present a novel method of aligning cells on biomaterials, simply by applying\n  an electrical current through the biomaterial.\n   \n  \u003cb\u003eSupplementary Figure S1 \u003c/b\u003e- Experimental setup for dip coating\n  multilayer films using a Gilson 223 Sample Changer converted for use as a dip\n  coater.\n   \n  \u003cb\u003eSupplementary Figure S2 \u003c/b\u003e- Scatter plot correlating the relationship\n  between the cell orientation (angle relative to the dipping direction of the\n  multilayer films) and length of the cells on the polymer films without and\n  with electrical stimulation. A) PEDOT-PSS-based films. B) PEDOT-S-based\n  films. Black circles represent cells without electrical stimulation, whereas\n  red circles represent cells with electrical stimulation; black and red lines\n  represent the corresponding trend lines.\n   \n  \u003cb\u003e \u003c/b\u003e\u003cb\u003eSupplementary Figure S3\u003c/b\u003e\n  HUMAN MESENCHYMAL STEM CELL\n  ADHESION STUDIES\n  HMSCs were supplied by Lonza\n  (Walkersville, MD). Samples were prepared as described above. After\n  sterilization, the samples were incubated for 30 minutes in 24 well plates\n  containing HMSC growth medium that was composed of: high glucose Dulbecco’s\n  Modified Eagle Medium (DMEM, 440 mL); fetal bovine serum (50 mL);\n  antibiotic-antimycotic (5 mL); non-essential amino acids (5 mL), and 2 ng\n  mL-1 basic fibroblast growth factor. Medium was aspirated and replaced prior\n  to HMSC seeding. Cell viability before starting the experiment was determined\n  by the Trypan Blue exclusion method, and the measured viability exceeded 95 %\n  in all cases. HMSCs were seeded at 5,000 cells cm-2, and incubated at 37 °C,\n  95 % humidity, and a CO2 content of 5 %. Samples were fixed and stained as\n  decribed above (n = 3).\n  \u003cb\u003e \u003c/b\u003e- HMSCs on multilayer\n  films after 3 days in culture. A) PEDOT-PSS-based multilayer films. B)\n  PEDOT-S-based multilayer films. DAPI-stained nuclei are blue and Alexa Fluor®\n  488-stained actin is green. Scale bars represent 150 µm.\n   \n  \u003cb\u003eSupplementary ZIP file \u003c/b\u003e– LabVIEW script and associated files for\n  control of Gilson 223 Sample Changer as a dip coater","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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