{"data":{"id":"10.25384/sage.c.4127561.v1","type":"dois","attributes":{"doi":"10.25384/sage.c.4127561.v1","prefix":"10.25384","suffix":"sage.c.4127561.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"E.E. Smith","affiliation":[],"nameIdentifiers":[]},{"name":"S. Angstadt","affiliation":[],"nameIdentifiers":[]},{"name":"N. Monteiro","affiliation":[],"nameIdentifiers":[]},{"name":"W. Zhang","affiliation":[],"nameIdentifiers":[]},{"name":"A. Khademhosseini","affiliation":[],"nameIdentifiers":[]},{"name":"P.C. Yelick","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Bioengineered Tooth Buds Exhibit Features of Natural Tooth Buds"}],"publisher":"Figshare","container":{},"publicationYear":2018,"subjects":[{"subject":"110599 Dentistry not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"91299 Materials Engineering not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Materials engineering","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2018-06-08","dateType":"Created"},{"date":"2018-06-08","dateType":"Updated"},{"date":"2018","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1177/0022034518779075","relatedIdentifierType":"DOI"},{"relationType":"IsIdenticalTo","relatedIdentifier":"10.25384/sage.c.4127561","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"CC BY 4.0","rightsUri":"https://creativecommons.org/licenses/by/4.0"}],"descriptions":[{"description":"Tooth loss is a significant health issue currently affecting millions of people worldwide. Artificial dental implants, the current gold standard tooth replacement therapy, do not exhibit many properties of natural teeth and can be associated with complications leading to implant failure. Here we propose bioengineered tooth buds as a superior alternative tooth replacement therapy. We describe improved methods to create highly cellularized bioengineered tooth bud constructs that formed hallmark features that resemble natural tooth buds such as the dental epithelial stem cell niche, enamel knot signaling centers, transient amplifying cells, and mineralized dental tissue formation. These constructs were composed of postnatal dental cells encapsulated within a hydrogel material that were implanted subcutaneously into immunocompromised rats. To our knowledge, this is the first report describing the use of postnatal dental cells to create bioengineered tooth buds that exhibit evidence of these features of natural tooth development. We propose future bioengineered tooth buds as a promising, clinically relevant tooth replacement therapy.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://figshare.com/collections/Bioengineered_Tooth_Buds_Exhibit_Features_of_Natural_Tooth_Buds/4127561/1","contentUrl":null,"metadataVersion":1,"schemaVersion":"http://datacite.org/schema/kernel-3","source":"api","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":1,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2018-06-08T12:01:58.000Z","registered":"2018-06-08T12:01:58.000Z","published":"2018","u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