{"data":{"id":"10.25384/sage.c.4123454","type":"dois","attributes":{"doi":"10.25384/sage.c.4123454","prefix":"10.25384","suffix":"sage.c.4123454","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"N. Odagaki","affiliation":[],"nameIdentifiers":[]},{"name":"Y. Ishihara","affiliation":[],"nameIdentifiers":[]},{"name":"Z. Wang","affiliation":[],"nameIdentifiers":[]},{"name":"E. Ei Hsu Hlaing","affiliation":[],"nameIdentifiers":[]},{"name":"M. Nakamura","affiliation":[],"nameIdentifiers":[]},{"name":"M. Hoshijima","affiliation":[],"nameIdentifiers":[]},{"name":"S. Hayano","affiliation":[],"nameIdentifiers":[]},{"name":"N. Kawanabe","affiliation":[],"nameIdentifiers":[]},{"name":"H. Kamioka","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Role of Osteocyte-PDL Crosstalk in Tooth Movement via SOST/Sclerostin"}],"publisher":"Figshare","container":{},"publicationYear":2018,"subjects":[{"subject":"110599 Dentistry not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"91299 Materials Engineering not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Materials engineering","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2018-06-05","dateType":"Created"},{"date":"2018-06-05","dateType":"Updated"},{"date":"2018","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1177/0022034518771331","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"CC BY 4.0","rightsUri":"https://creativecommons.org/licenses/by/4.0"}],"descriptions":[{"description":"Sclerostin (Scl) negatively regulates bone formation and favors bone resorption. Osteocytes, the cells responsible for mechanosensing, are known as the primary source of Scl and are a key regulator of bone remodeling through the induction of receptor activator of NF-κB ligand (RANKL). However, the spatiotemporal patterns of Scl in response to mechanical stimuli and their regulatory mechanisms remain unknown. We investigated the regulatory dynamics of the \u003ci\u003eSOST\u003c/i\u003e/Scl expression generated by orthodontic tooth movement (OTM) in vivo and in vitro. In 8-wk-old male mice, coil springs were used to move the first molar mesially for 0, 1, 5, or 10 d. A regional histogram and the distribution patterns of the Scl expression showed that the Scl expression in the alveolar bone was increased on the compression side and peaked on day 5, with a gradual increase in the degree of significance. On day 10, the expression around the periodontal ligament (PDL)–alveolar bone boundary returned to the control level. Conversely, the expression of Scl on the tension side was only significantly decreased on day 1. Compressive force biphasically modulated the \u003ci\u003eSOST\u003c/i\u003e/Scl expression in the isolated human PDL and thereby upregulated osteocytic \u003ci\u003eSOST\u003c/i\u003e via paracrine activation in an osteocyte-PDL co-culture system designed to mimic OTM. This system did not affect the \u003ci\u003eRANKL\u003c/i\u003e or \u003ci\u003eOPG\u003c/i\u003e expression in osteocytes, suggesting that the bone resorption pathways are acted upon in a PDL-dependent and osteocyte-independent manner through RANKL/OPG signaling. Moreover, sclerostin neutralizing antibody significantly attenuated the upregulation of \u003ci\u003eSOST\u003c/i\u003e that was induced by compressive force. In conclusion, our results provide evidence to support that factors secreted by the PDL, including \u003ci\u003eSOST\u003c/i\u003e/Scl, control alveolar bone remodeling through osteocytic \u003ci\u003eSOST\u003c/i\u003e/Scl in OTM.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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