{"data":{"id":"10.25384/sage.c.4095620.v1","type":"dois","attributes":{"doi":"10.25384/sage.c.4095620.v1","prefix":"10.25384","suffix":"sage.c.4095620.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"X. He","affiliation":[],"nameIdentifiers":[]},{"name":"F. Li","affiliation":[],"nameIdentifiers":[]},{"name":"B. Bor","affiliation":[],"nameIdentifiers":[]},{"name":"K. Koyano","affiliation":[],"nameIdentifiers":[]},{"name":"L. Cen","affiliation":[],"nameIdentifiers":[]},{"name":"X. Xiao","affiliation":[],"nameIdentifiers":[]},{"name":"W. Shi","affiliation":[],"nameIdentifiers":[]},{"name":"D.T.W. Wong","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Human tRNA-Derived Small RNAs Modulate Host–Oral Microbial Interactions"}],"publisher":"Figshare","container":{},"publicationYear":2018,"subjects":[{"subject":"110599 Dentistry not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"91299 Materials Engineering not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Materials engineering","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2018-05-09","dateType":"Created"},{"date":"2018-05-09","dateType":"Updated"},{"date":"2018","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1177/0022034518770605","relatedIdentifierType":"DOI"},{"relationType":"IsIdenticalTo","relatedIdentifier":"10.25384/sage.c.4095620","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"CC BY 4.0","rightsUri":"https://creativecommons.org/licenses/by/4.0"}],"descriptions":[{"description":"Coevolution of the human host and its associated microbiota has led to sophisticated interactions to maintain a delicate homeostasis. Emerging evidence suggests that in addition to small molecules, peptides, and proteins, small regulatory noncoding RNAs (sRNAs) might play an important role in cross-domain interactions. In this study, we revealed the presence of diverse host transfer RNA–derived small RNAs (tsRNAs) among human salivary sRNAs. We selected 2 tsRNAs (tsRNA-000794 and tsRNA-020498) for further study based on their high sequence similarity to specific tRNAs from a group of Gram-negative oral bacteria, including \u003ci\u003eFusobacterium nucleatum\u003c/i\u003e, a key oral commensal and opportunistic pathogen. We showed that the presence of \u003ci\u003eF. nucleatum\u003c/i\u003e triggers exosome-mediated release of tsRNA-000794 and tsRNA-020498 by human normal oral keratinocyte cells. Furthermore, both tsRNA candidates exerted a growth inhibition effect on \u003ci\u003eF. nucleatum\u003c/i\u003e, likely through interference with bacterial protein biosynthesis, but did not affect the growth of \u003ci\u003eStreptococcus mitis\u003c/i\u003e, a health-associated oral Gram-positive bacterium whose genome does not carry sequences bearing high similarity to either tsRNA. Our data provide the first line of evidence for the modulatory role of host-derived tsRNAs in the microbial-host interaction.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://figshare.com/collections/Human_tRNA-Derive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