{"data":{"id":"10.25384/sage.c.4056803","type":"dois","attributes":{"doi":"10.25384/sage.c.4056803","prefix":"10.25384","suffix":"sage.c.4056803","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"R.B.P. Miranda","affiliation":[],"nameIdentifiers":[]},{"name":"L. Grenho","affiliation":[],"nameIdentifiers":[]},{"name":"A. Carvalho","affiliation":[],"nameIdentifiers":[]},{"name":"M.H. Fernandes","affiliation":[],"nameIdentifiers":[]},{"name":"F.J. Monteiro","affiliation":[],"nameIdentifiers":[]},{"name":"P.F. Cesar","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Micropatterned Silica Films with Nanohydroxyapatite for Y-TZP Implants"}],"publisher":"Figshare","container":{},"publicationYear":2018,"subjects":[{"subject":"110599 Dentistry not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"91299 Materials Engineering not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Materials engineering","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2018-04-03","dateType":"Created"},{"date":"2018-04-03","dateType":"Updated"},{"date":"2018","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1177/0022034518765762","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"CC BY 4.0","rightsUri":"https://creativecommons.org/licenses/by/4.0"}],"descriptions":[{"description":"This investigation aimed at developing micropatterned silica thin films (MSTFs) containing nanohydroxyapatite (nano-HA) microaggregates that were not completely covered by silica so that they could directly interact with the surrounding cells. The objectives were 1) to evaluate the effect of the presence of 2 films (MSTF with or without nano-HA addition) on the characteristic strength (σ\u003csub\u003e0\u003c/sub\u003e) and Weibull modulus (\u003ci\u003em\u003c/i\u003e) of a yttria-stabilized tetragonal zirconia polycrystal (Y-TZP) and 2) to evaluate the effect of these 2 films, as applied onto the Y-TZP surface, on the morphology, orientation, and proliferation of MG63 cells. Sol-gel process and soft lithography were used to apply the MSTF onto the Y-TZP specimens. Three experimental groups were produced: Y-TZP, Y-TZP + MSTF, and Y-TZP + MSTF + sprayed nano-HA. All surfaces were characterized by scanning electron microscopy and energy-dispersive X-ray spectroscopy and tested for 4-point flexural strength (\u003ci\u003en\u003c/i\u003e = 30) in water at 37 °C. Weibull analysis was used to determine \u003ci\u003em\u003c/i\u003e and σ\u003csub\u003e0\u003c/sub\u003e (maximum likelihood method). In vitro biological behavior was performed with human osteoblast-like cells (MG63). Y-TZP was successfully coated with MSFT and MSFT + nano-HA. Scanning electron microscopy micrographs indicated that the microaggregates of nano-HA were not entirely covered by the silica. There was no statistically significant difference among the experimental groups for σ\u003csub\u003e0\u003c/sub\u003e and \u003ci\u003em\u003c/i\u003e. In the groups containing the films, the cells were elongated and aligned along the lines. The MSFT + nano-HA group showed significantly higher cell metabolic activity than that obtained for the Y-TZP group at day 7. This investigation was successful in producing an MSTF containing nano-HA microaggregates that remained exposed to the environment. The developed films did not jeopardize the structural reliability of a commercial Y-TZP, as confirmed by the Weibull statistics. The MG63 cells seeded over the films became elongated and aligned along the films’ micropatterned lines. Y-TZP specimens coated with MSTF and nano-HA showed a higher cell metabolic activity and proliferation after 7 d of culture when compared with uncoated Y-TZP.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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