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While numerous other cancers now have promising\n therapeutic advances with immunotherapies, targeted therapies, and stem\n cells, treatment options for patients with OS have remained unchanged for\n more than four decades since the advent of standard chemotherapeutics and\n offer less than a 25% 5-year survival rate for those with metastatic\n disease. This dearth of clinical progress underscores a lack of\n understanding of OS progression and metastagenesis and necessitates the\n study of this disease in a novel system. Here, we adapt a previously\n described engineered bone marrow (eBM) construct for use as a 3D platform\n to study how microenvironmental and immune factors affect OS tumor\n progression. We form eBM by implanting acellular bone-forming materials in\n mice and explanting the cellularized constructs for study after eight\n weeks. We interrogate the influence of the anatomical implantation site on\n the quality of generated tissue, test ex vivo stability under normoxic (5%\n O2) and standard (21% O2) culture conditions, and culture OS cells within\n these eBM constructs. We show that eBM accurately and stably recapitulates\n the composition of native bone marrow. OS cells exhibit differential\n behavior dependent on metastatic potential when cultured in eBM, thus\n closely mimicking in vivo conditions. Furthermore, we highlight the\n clinical applicability of eBM as a drug-screening platform through\n doxorubicin treatment of OS cultured in monolayer, Matrigel, or eBM, and\n we show that eBM confers a protective effect on OS cells that parallel\n clinical responses. Combined, this work presents eBM as a cellular\n construct that mimics the complex bone marrow environment that may be\n useful for mechanistic bone cancer research and drug screening.","descriptionType":"Abstract"},{"description":"eBM was minced into \u0026lt;= 0.5mm cubes and collected in 500\n micro liter of TRIzol Reagent (Invitrogen). Samples were then homogenized\n with a Tissue-tearor (BioSpec, Bartlesville, OK) at highest speed for 10 s\n intervals totaling 30 s, followed by sonication for another 10 s.\n Following RNA isolation, sequencing libraries were prepared using Illumina\n Stranded mRNA Prep kit using manufacturer's recommendations and then\n sequenced on an Illumina NextSeq 500 sequencer. Conversion of Illumina\n base calls to sequences and sample demultiplexing was performed using\n bcl2fastq.","descriptionType":"Methods"}],"geoLocations":[],"fundingReferences":[{"funderName":"Musculoskeletal Tumor Society*","awardNumber":"A23-0879"}],"xml":"<?xml version="1.0" encoding="UTF-8"?>
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      <creatorName nameType="Personal">Griffin, Katherine</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/05rrcem69" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of California, Davis</affiliation>
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    <creator>
      <creatorName nameType="Personal">Thorpe, Steven</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/05rrcem69" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of California, Davis</affiliation>
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    <creator>
      <creatorName nameType="Personal">Sebastian, Aimy</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/041nk4h53" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Lawrence Livermore National Laboratory</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Hum, Nicholas</creatorName>
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      <affiliation affiliationIdentifier="https://ror.org/041nk4h53" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">Lawrence Livermore National Laboratory</affiliation>
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      <creatorName nameType="Personal">Coonan, Thomas</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/05rrcem69" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of California, Davis</affiliation>
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    <creator>
      <creatorName nameType="Personal">Sagheb, Isabel</creatorName>
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    <creator>
      <creatorName nameType="Personal">Loots, Gabriela</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/05rrcem69" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of California, Davis</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Randall, Lor</creatorName>
      <affiliation affiliationIdentifier="https://ror.org/05rrcem69" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of California, Davis</affiliation>
    </creator>
    <creator>
      <creatorName nameType="Personal">Leach, Kent</creatorName>
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      <affiliation affiliationIdentifier="https://ror.org/05rrcem69" affiliationIdentifierScheme="ROR" schemeURI="https://ror.org">University of California, Davis</affiliation>
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  <titles>
    <title>
      Bulk RNAseq of K7M2 osteosarcoma cells in engineered bone marrow treated
      with Doxorubicin
    </title>
  </titles>
  <publisher publisherIdentifier="https://ror.org/00x6h5n95" publisherIdentifierScheme="ROR" schemeURI="https://ror.org/">Dryad</publisher>
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    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Osteosarcoma</subject>
    <subject>bulk RNA sequencing</subject>
    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Gene expression</subject>
    <subject subjectScheme="PLOS Subject Area Thesaurus" schemeURI="https://github.com/PLOS/plos-thesaurus">Inflammation</subject>
  </subjects>
  <fundingReferences>
    <fundingReference>
      <funderName>Musculoskeletal Tumor Society*</funderName>
      <awardNumber>A23-0879</awardNumber>
    </fundingReference>
  </fundingReferences>
  <dates>
    <date dateType="Created">2023-08-22T22:20:30Z</date>
    <date dateType="Submitted">2023-08-22T22:33:01Z</date>
    <date dateType="Issued">2023-09-01T00:00:00Z</date>
    <date dateType="Available">2023-09-01T00:00:00Z</date>
  </dates>
  <language>en</language>
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  <descriptions>
    <description descriptionType="Abstract">
      Osteosarcoma (OS) is the most common primary malignant bone cancer in
      children and adolescents. While numerous other cancers now have promising
      therapeutic advances with immunotherapies, targeted therapies, and stem
      cells, treatment options for patients with OS have remained unchanged for
      more than four decades since the advent of standard chemotherapeutics and
      offer less than a 25% 5-year survival rate for those with metastatic
      disease. This dearth of clinical progress underscores a lack of
      understanding of OS progression and metastagenesis and necessitates the
      study of this disease in a novel system. Here, we adapt a previously
      described engineered bone marrow (eBM) construct for use as a 3D platform
      to study how microenvironmental and immune factors affect OS tumor
      progression. We form eBM by implanting acellular bone-forming materials in
      mice and explanting the cellularized constructs for study after eight
      weeks. We interrogate the influence of the anatomical implantation site on
      the quality of generated tissue, test ex vivo stability under normoxic (5%
      O2) and standard (21% O2) culture conditions, and culture OS cells within
      these eBM constructs. We show that eBM accurately and stably recapitulates
      the composition of native bone marrow. OS cells exhibit differential
      behavior dependent on metastatic potential when cultured in eBM, thus
      closely mimicking in vivo conditions. Furthermore, we highlight the
      clinical applicability of eBM as a drug-screening platform through
      doxorubicin treatment of OS cultured in monolayer, Matrigel, or eBM, and
      we show that eBM confers a protective effect on OS cells that parallel
      clinical responses. Combined, this work presents eBM as a cellular
      construct that mimics the complex bone marrow environment that may be
      useful for mechanistic bone cancer research and drug screening.
    </description>
    <description descriptionType="Methods">
      &lt;div&gt;eBM was minced into &amp;lt;= 0.5mm cubes and collected in 500
      micro liter of TRIzol Reagent (Invitrogen). Samples were then homogenized
      with a Tissue-tearor (BioSpec, Bartlesville, OK) at highest speed for 10 s
      intervals totaling 30 s, followed by sonication for another 10 s.
      Following RNA isolation, sequencing libraries were prepared using Illumina
      Stranded mRNA Prep kit using manufacturer's recommendations and then
      sequenced on an Illumina NextSeq 500 sequencer. Conversion of Illumina
      base calls to sequences and sample demultiplexing was performed using
      bcl2fastq.&lt;/div&gt;
    </description>
  </descriptions>
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